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Evaluation and modelling the utility of SCCO2 to support efficient lipase mediated esterification.

Abstract：

:Supercritical fluids offer environmental advantages over chemical solvents, while providing enhanced separation and chemical selectivity. The use of supercritical fluids for the recovery of products from biomass and the transformation of selected molecules (to add value) was studied. Free fatty acids were bio-catalytically transformed to fatty acid esters using lipase within a supercritical fluid environment. A central composite rotatable design was used to evaluate the influence of operating conditions on the enzymatic esterification process and a response surface equation was optimized to identify the most favourable process conditions for maximum free fatty acid conversion. Based on the model equation the process conditions under which it was predicted a yield of 100% esters could be obtained were: pressure 200 bar, temperature 60 °C, ethanol concentration 2.0 M, enzyme concentration 11 wt.% and time 60 min. Experiments conducted under these conditions gave an ester yield of 94.3% (close to predicted results). The activity per unit mass of biocatalyst was found to be 1585 μmol/min/g(cat). The results support the use of supercritical fluids for process integration.

journal_name

Enzyme Microb Technol

journal_title

Enzyme and microbial technology

authors

Baig MN,Santos RC,Zetzl C,King J,Pioch D,Bowra S

doi

10.1016/j.enzmictec.2011.07.004

subject

Has Abstract

pub_date

2011-09-10 00:00:00

pages

420-6

issue

4

eissn

0141-0229

issn

1879-0909

pii

S0141-0229(11)00164-5

journal_volume

49

pub_type

杂志文章

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