Effect of different carbon sources on lipase production by Candida rugosa.

Abstract:

:Different carbon sources affecting growth and lipase production in Candida rugosa were studied by using batch cultures on defined medium. Carbohydrates and acids non-related to fats did not induce lipase production. The highest yields of enzyme were obtained with lipids or fatty acids as carbon sources. Tween 80 stimulated lipase biosynthesis and secretion outside the cell. Combinations of two types of substrates, carbohydrates and fatty acids, did not improve lipase production, and in some cases, their consumption was produced in a sequential pattern. Glucose presented a repressing effect on lipase production. Moreover, glucose was found to be effective in stimulating lipase secretion by cells with a high level of cell-bound lipase activity because of their previous growth in oleic acid.

journal_name

Enzyme Microb Technol

authors

Dalmau E,Montesinos JL,Lotti M,Casas C

doi

10.1016/s0141-0229(00)00156-3

keywords:

subject

Has Abstract

pub_date

2000-06-01 00:00:00

pages

657-663

issue

9-10

eissn

0141-0229

issn

1879-0909

pii

S0141022900001563

journal_volume

26

pub_type

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