Construction of glutamate biosensor based on covalent immobilization of glutamate oxidase on polypyrrole nanoparticles/polyaniline modified gold electrode.


:A method is described for construction of a highly sensitive electrochemical biosensor for detection of glutamate. The biosensor is based on covalent immobilization of glutamate oxidase (GluOx) onto polypyrrole nanoparticles and polyaniline composite film (PPyNPs/PANI) electrodeposited onto Au electrode. The enzyme electrode was characterized by cyclic voltammetry (CV), scanning electron microscopy (SEM), X-ray diffraction (XRD), transmission electron microscopy (TEM), Fourier transform infra-red spectroscopy (FTIR) and electrochemical impedance spectroscopy (EIS). The biosensor showed optimum response within 3s at pH 7.5 (0.1 M sodium phosphate) and 35 °C, when operated at 50 mV s⁻¹. It exhibited excellent sensitivity (detection limit as 0.1 nM), fast response time and wider linear range (from 0.02 to 400 μM). Analytical recovery of added glutamate (5 mM and 10 mM) was 95.56 and 97%, while within batch and between batch coefficients of variation were 3.2% and 3.35% respectively. The enzyme electrode was used 100 times over a period of 60 days, when stored at 4 °C. The biosensor measured glutamate level in food stuff, which correlated well with a standard colorimetric method (r=0.99).


Enzyme Microb Technol


Batra B,Kumari S,Pundir CS




Has Abstract


2014-04-10 00:00:00












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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Scott SD,Armenta RE,Berryman KT,Norman AW

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    journal_title:Enzyme and microbial technology

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    authors: Völler JS,Thi To TM,Biava H,Koksch B,Budisa N

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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Dagar K,Pundir CS

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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Matsumoto T,Shimada S,Hata Y,Tanaka T,Kondo A

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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Shaughnessy TS,Kargi F

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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


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    journal_title:Enzyme and microbial technology

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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Frampton MB,Zelisko PM

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    pub_type: 杂志文章


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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Monrroy M,Ortega I,Ramírez M,Baeza J,Freer J

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    authors: Fernandez-Lafuente R,Rosell CM,Guisán JM

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    journal_title:Enzyme and microbial technology

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  • Characterization and solvent engineering of wheat β-amylase for enhancing its activity and stability.

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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Xia HQ,Sakai K,Kitazumi Y,Shirai O,Takagi K,Kano K

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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


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    journal_title:Enzyme and microbial technology

    pub_type: 杂志文章


    authors: Lin Y,Jin W,Wang J,Cai Z,Wu S,Zhang G

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