Abstract：
:This work describes for the first time the green synthesis of neopentyl glycol diheptanoate in a solvent-free medium via an enzymatic pathway. The process has been carried out in an open-air reactor in order to ease water removal through evaporation and shift the chemical equilibrium towards product formation. The inhibiting effect of high concentrations of heptanoic acid has been put into evidence by a reduction of initial reaction rate when esterification was performed with stoichiometric amounts of substrates. Therefore, in this work different strategies for the stepwise addition of heptanoic acid are proposed, and best results were obtained when stoichiometric quantities of acid were divided in four equal amounts and added when previous batch was consumed. Biocatalyst Novozym® 435 concentration and temperature were optimised, giving yields of 90% in neopentyl glycol diheptanoate when 7.5% (w/w) and 70 °C were used. With a remaining 7% of heptanoic acid (probably caused by the alcohol evaporation) the addition of neopentyl glycol led to a conversion of 95%. Thus, product can be used in cosmetics without further purification and can be labelled as environmentally-friendly synthesized because of its enzymatic origin.
journal_name
Enzyme Microb Technoljournal_title
Enzyme and microbial technologyauthors
Serrano-Arnaldos M,García-Martínez JJ,Ortega-Requena S,Bastida J,Máximo F,Montiel MCdoi
10.1016/j.enzmictec.2019.109400subject
Has Abstractpub_date
2020-01-01 00:00:00pages
109400eissn
0141-0229issn
1879-0909pii
S0141-0229(19)30138-3journal_volume
132pub_type
杂志文章abstract：:The xylanase R8 gene (xynR8) from uncultured rumen fungi was cloned and successfully expressed in Lactobacillus reuteri. A xylanase activity of 132.1 U/mL was found in the broth of L. reuteri R8, the transformant containing pNZ3004 vector with xynR8 gene insertion. Two distinct forms of recombinant xylanase with diffe...
journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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更新日期：2020-03-01 00:00:00
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
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更新日期：2000-03-01 00:00:00
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2013.01.005
更新日期：2013-04-10 00:00:00
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/j.enzmictec.2011.04.018
更新日期：2011-07-10 00:00:00
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journal_title：Enzyme and microbial technology
pub_type： 杂志文章
doi：10.1016/0141-0229(93)90065-a
更新日期：1993-08-01 00:00:00
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journal_title：Enzyme and microbial technology
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更新日期：2011-06-10 00:00:00
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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更新日期：2015-02-01 00:00:00
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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journal_title：Enzyme and microbial technology
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更新日期：1990-09-01 00:00:00
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journal_title：Enzyme and microbial technology
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更新日期：2019-03-01 00:00:00
