Abstract：
:Brain extracts from 3 to 4 day old final (eighth) instar larvae of Opisina arenosella (Lepidoptera) stimulate amylase release from midgut preparations maintained in vitro. This effect of the brain extract was both time and dose dependent. The brain factor stimulating enzyme release may be a peptide as it is heat stable and susceptible to treatment with proteolytic enzymes. For purification of the brain factor, a head extract prepared in 2% NaCl was first precipitated in 80% aqueous acetone and then fractionated by DEAE cellulose ion exchange chromatography. The fraction OCF(2), from ion exchange chromatography was further purified on a Sephadex G25 column. The fraction designated as OCF(2.3) obtained by gel filtration showed maximum activity and it was selected for HPLC analysis. HPLC elution profiles of OCF(2.3) showed two major peaks separated by a time interval of 0.107 min. The two overlapping peaks of OCF(2.3) may represent either different forms of a peptide or different peptides of a family. The molecular weight OCF(2.3) was estimated to be 1070 Da.
journal_name
Neuropeptidesjournal_title
Neuropeptidesauthors
Harshini S,Reshmi V,Sreekumar Sdoi
10.1016/s0143-4179(03)00025-8keywords:
subject
Has Abstractpub_date
2003-06-01 00:00:00pages
133-9issue
3eissn
0143-4179issn
1532-2785pii
S0143417903000258journal_volume
37pub_type
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