Modulation of CCK mRNA in cell lines in response to isoproterenol and retinoic acid.


:Regulation of cholecystokinin (CCK) expression was studied in the human neuroepithelioma cell line SK-N-MCIXC and the rat medullary thyroid carcinoma cell line WE 4/2. The cells were treated with the beta-adrenergic agonist isoproterenol and retinoic acid, a natural derivative of vitamin A, which plays a role in cell growth and proliferation. Levels of CCK mRNA were determined after 6, 12 and 24 h drug treatments, with Northern blot analysis using human CCK riboprobes. In WE 4/2 cells no differences were observed in CCK mRNA levels, between control and isoproterenol treated cells, after 6, 12 or 24 h treatments. In SK-N-MCIXC cells isoproterenol increased CCK mRNA levels at all time points examined, the beta-adrenergic antagonist propranolol blocked this effect. SK-N-MCIXC cells were also treated with actinomycin D or cycloheximide in combination with isoproterenol. Actinomycin D decreased CCK mRNA levels. Cycloheximide increased CCK mRNA levels when compared to isoproterenol acting alone. Retinoic acid did not affect CCK mRNA levels in WE 4/2 cells. In SK-N-MCIXC cells, retinoic acid consistently decreased CCK mRNA level. CCK mRNA levels in SK-N-MCIXC cells treated with retinoic acid combined with either isoproterenol or phorbol-12-myristate-13 acetate, were not significantly different from cells treated with retinoic acid alone.






Mania-Farnell BL,Botros IW,Davis TP




Has Abstract


1995-10-01 00:00:00














  • Effect of intraperitoneal mRNA antisense-oligodeoxynucleotides to cholecystokinin on anxiety-like and learning behaviors in rats: association with pre-experimental stress.

    abstract:RATIONALE:Cholecystokinin and its analogs generate anxiety in humans and measurable anxiety-like behaviors in rats. Cholecystokinin receptor blockers have been reported to have variable effects in the treatment of anxiety disorders. We demonstrated that intracerebroventricular administration of Cholecystokinin-antisens...


    pub_type: 杂志文章


    authors: Cohen H,Matar MA,Buriakovsky I,Zeev K,Kotler M,Bourin M

    更新日期:2002-10-01 00:00:00

  • Effects of renal denervation on renin release induced by intracerebroventricular administration of biological active peptides in conscious rats.

    abstract::Experiments were performed to determine whether the renal nerve mediates the renin release induced by intracerebroventricular administration of angiotensin II (ANG II), bradykinin (BK), leucine-enkephalin (Leu-ENK) and neurotensin (NT). In sham-operated rats, both ANG II and NT suppressed plasma renin activity (PRA), ...


    pub_type: 杂志文章


    authors: Iwata T,Hiwada K,Kokubu T

    更新日期:1985-09-01 00:00:00

  • [3H]-Dynorphin A (1-8): degradation profile and binding characteristics in brain homogenates.

    abstract::[3H]-Dynorphin A (1-8) was degraded in brain homogenates at 25 degrees and even at 0 degree C. The peptidase inhibitors bestatin and captopril almost completely protected[3H]-dynorphin A (1-8) from degradation at 0 degree C but had only little effect on binding at this temperature. At 25 degrees C, the binding of [3H]...


    pub_type: 杂志文章


    authors: Gillan MG,Robson LE,McKnight AT,Kosterlitz HW

    更新日期:1985-02-01 00:00:00

  • Nerve growth factor induces a succession of increases in isoprenylated methylated small GTP-binding proteins of PC-12 pheochromocytoma cells.

    abstract::Pheochromocytoma (PC-12) cells exposed to nerve growth factor (NGF) acquire a sympathetic neuron-like phenotype. This NGF-response is blocked by methylation inhibitors and can be mimicked by the farnesylated methylated small GTP-binding protein p21ras. The implicated involvement of prenylation, methylation and a small...


    pub_type: 杂志文章


    authors: Haklai R,Lerner S,Kloog Y

    更新日期:1993-01-01 00:00:00

  • Neuropeptide Y-like immunoreactivity in lumbar cerebrospinal fluid of patients after severe head trauma.

    abstract::We studied head-injured patients assessed after admission to the hospital according to the Glasgow coma scale (3-8 points). All of them underwent surgical procedure after neuroradiological examination. Due to therapeutic problems, some of these patients had lumbar puncture on the fourth day following surgery. The cont...


    pub_type: 杂志文章


    authors: Stachura Z,Obuchowicz E,Herman ZS

    更新日期:1997-02-01 00:00:00

  • Cardioacceleration provoked by intrathecal administration of vasoactive intestinal peptide (VIP): mediation by a non-central nervous system mechanism.

    abstract::Intrathecal administration of VIP to the thoracic spinal cord in the urethane anaesthetized rat provoked a dose-dependent increase in heart rate without any change in arterial pressure. The cardioacceleration observed following administration of 6.5 nmol of VIP at the T9 level (n = 8) occurred within 1-2 min of admini...


    pub_type: 杂志文章


    authors: Rochford J,Henry JL

    更新日期:1989-11-01 00:00:00

  • Role of tachykinins and neurokinin receptor subtypes in the regulation of motility of the forestomach and abomasum in conscious sheep.

    abstract::The present study was planned to evaluate role of tachykinins (TKs) and neurokinin (NK) receptors in the regulation of gastric motility in sheep. We examined the effects of intravenous (i.v.) injection of neurokinin A (NKA) and substance P (SP) on motility of the rumen, omasum, and abomasum in conscious sheep and the ...


    pub_type: 杂志文章


    authors: Oh-ishi T,Shimoda T,Hayashi H,Onaga T

    更新日期:2013-02-01 00:00:00

  • Modulatory role of neuropeptide FF system in nociception and opiate analgesia.

    abstract::The tetra-peptide FMRF-NH(2) is a cardioexcitatory peptide in the clam. Using the antibody against this peptide, FMRF-NH(2)-like immunoreactive material was detected in mammalian CNS. Subsequently, mammalian FMRF-NH(2) immunoreactive peptides were isolated from bovine brain and characterized to be FLFQPQRF-NH(2) (NPFF...


    pub_type: 杂志文章,评审


    authors: Yang HY,Tao T,Iadarola MJ

    更新日期:2008-02-01 00:00:00

  • A putative morphological substrate of the catecholamine-influenced neuropeptide Y (NPY) release in the human hypothalamus.

    abstract::Neuropeptide Y (NPY) is a 36 amino acid peptide, which among others, plays a pivotal role in stress response. Although previous studies confirmed that NPY release is increased by stress in several species, the exact mechanism of the stress-induced NPY release has not been elucidated yet. In the present study, we exami...


    pub_type: 杂志文章


    authors: Ko L,Rotoli G,Grignol G,Hu W,Merchenthaler I,Dudas B

    更新日期:2011-06-01 00:00:00

  • Electroencephalographic assessment of the role of delta receptors in opioid peptide - induced seizures.

    abstract::DPDPE ([D-Pen2, D-Pen5]-Enkephalin) and DPLPE ([D-Pen2, L-Pen5]-Enkephalin) are conformationally-constrained cyclic analogs of enkephalin with high selectivity for delta opioid receptors. Intracerebroventricular (i.c.v.) administration of each analog acutely produces a complex EEG response in rats characterized by a d...


    pub_type: 杂志文章


    authors: Tortella FC,Robles L,Mosberg HI,Holaday JW

    更新日期:1984-12-01 00:00:00

  • Effect of vasopressin, oxytocin and peptides derived from these hormones on field potential induced in lateral septum of rats by stimulation of the fimbria fornix.

    abstract::Superfusion of the dorsal surface of the septum with artificial cerebrospinal fluid containing 10(-10) M [Arg8]-vasopressin (VP) significantly increased the negative (N) wave in field potentials (FPs) elicited in the lateral septum (LS) of rats by stimulation of the fimbria-fornix. A similar increase in the FPs negati...


    pub_type: 杂志文章


    authors: Urban IJ,De Wied D

    更新日期:1986-01-01 00:00:00

  • The analgesic effect of orexin-A in a murine model of chemotherapy-induced neuropathic pain.

    abstract::Orexins are neuropeptides that are localized to neurons in the lateral and dorsal hypothalamus but its receptors are distributed to many different regions of the central nervous system. Orexins are implicated in a variety of physiological functions including sleep regulation, energy homeostats, and stress reactions. F...


    pub_type: 杂志文章


    authors: Toyama S,Shimoyama N,Shimoyama M

    更新日期:2017-02-01 00:00:00

  • Vascular neuropeptide Y Y1-receptors in the rat kidney: vasoconstrictor effects and expression of Y1-receptor mRNA.

    abstract::Neuropeptide Y (NPY) -receptor subtypes were studied in the rat kidney in vivo by systemic administration of NPY, the two agonists [Leu(31), Pro(34)]NPY (Y1-receptor agonist) and NPY (13-36) (Y2-receptor agonist), or the Y1-receptor antagonist BIBP 3226. Effects on mean arterial blood pressure (MAP) and renal arterial...


    pub_type: 杂志文章


    authors: Modin A,Malmström RE,Meister B

    更新日期:1999-08-01 00:00:00

  • Obestatin improve spatial memory impairment in a rat model of fetal alcohol spectrum disorders via inhibiting apoptosis and neuroinflammation.

    abstract::Clinical and experimental evidence have demonstrated that, use of alcohol during pregnancy can interrupt brain development. Alcohol-induced neurocognitive deficits in offspring's are involved with activation of oxidative-inflammatory cascade joined with extensive apoptotic neurodegeneration in different brain regions ...


    pub_type: 杂志文章


    authors: Toosi A,Shajiee H,Khaksari M,Vaezi G,Hojati V

    更新日期:2019-04-01 00:00:00

  • Effects of the peripherally selective kappa-opioid asimadoline, on substance P and CGRP mRNA expression in chronic arthritis of the rat.

    abstract::We have previously shown that the kappa-opioid agonist, asimadoline, produces time-dependent changes in neuropeptide concentrations in the joints of rats with chronic arthritis. We hypothesized that asimadoline acts on peripheral terminals to modulate substance P (SP) release. To address this hypothesis, here we have ...


    pub_type: 杂志文章


    authors: Walker JS,Scott C,Bush KA,Kirkham BW

    更新日期:2000-06-01 00:00:00

  • Chronic pain in the rat: selective alterations in CNS and pituitary pools of dynorphin as compared to vasopressin.

    abstract::Inoculation of rats at the tail-base with Mycobacterium led to arthritic swelling and inflammation of all four limbs. Immunoreactive (ir)-dynorphin (DYN) increased in anterior but not neurointermediate pituitary. In the brain, only thalamus showed a rise and, in spinal cord, a large elevation was seen. Ir-vasopressin ...


    pub_type: 杂志文章


    authors: Millan MJ,Millan MH,Pilcher CW,Colpaert FC,Herz A

    更新日期:1985-02-01 00:00:00

  • Opioid systems in the amygdala can serve as substrate for the behavioral effects of the ACTH-(4-9) analog ORG 2766.

    abstract::Rats housed individually for 7 days showed a marked decrease in motor activity when tested under intense light conditions in a novel environment as compared to group-housed rats tested under low light conditions. The ACTH analogue ORG 2766 administered into the amygdala decreased the motor activity of group-housed rat...


    pub_type: 杂志文章


    authors: Wolterink G,Van Ree JM

    更新日期:1989-08-01 00:00:00

  • Pharmacological and mechanistic studies of cholecystokinin-facilitated [3H]dopamine efflux from rat nucleus accumbens.

    abstract::Cholecystokinin (CCK) is co-localized with dopamine (DA) in mesolimbic neurons of the CNS and appears to selectively regulate the output of this system. In an attempt to characterize the nature of CCK interactions with mesolimbic DA-containing nerve terminals, we have investigated CCK regulation of [3H]DA overflow fro...


    pub_type: 杂志文章


    authors: Vickroy TW,Bianchi BR

    更新日期:1989-01-01 00:00:00

  • Buspirone and related compounds as alternative anxiolytics.

    abstract::The desire of the pharmaceutical industry to obtain more selective agents for the treatment of anxiety with fewer or diminished side effects and a profile consistent with safety during long-term use resulted in a search which has identified the azapirones as a new class of anxiolytics which lack structural or biochemi...


    pub_type: 临床试验,杂志文章,评审


    authors: Taylor DP,Moon SL

    更新日期:1991-07-01 00:00:00

  • CNTFR alpha alone or in combination with CNTF promotes macrophage chemotaxis in vitro.

    abstract::Microchemotaxis chambers were used to investigate whether one aspect of ciliary neurotrophic factor CNTF's role as a lesion factor might be to promote the initial early recruitment of macrophages, which express the signal transducing receptor components, gp130 and LIFRbeta. CNTFRalpha alone, or in combination with CNT...


    pub_type: 杂志文章


    authors: Kobayashi H,Mizisin AP

    更新日期:2000-12-01 00:00:00

  • Identification of endothelin receptors in cultured cerebellar neurons.

    abstract::We have described the binding of [125I]endothelin-1 (ET-1) to cultured rat cerebellar granule cells. Binding of [125I]ET-1 was specific, saturable, and time-dependent. Scatchard analysis of saturation binding data indicated a single class of high affinity binding site with a KD of 95 pM, and a Bmax of 8110 receptors/c...


    pub_type: 杂志文章


    authors: Lysko PG,Feuerstein G,Pullen M,Wu HL,Nambi P

    更新日期:1991-02-01 00:00:00

  • Neural and non-neural origin of calcitonin gene-related peptide (CGRP) in the gastric mucosa.

    abstract::We have used immunohistochemistry and in situ hybridization histochemistry to visualize CGRP and the mRNA encoding the CGRP precursor in the stomach. CGRP is present in nerve fibers in the mucosa. CGRP mRNA and CGRP itself are also found in non-neural cells in the lamina propria. These cells are likely to be macrophag...


    pub_type: 杂志文章


    authors: Jakab G,Webster HD,Salamon I,Mezey E

    更新日期:1993-02-01 00:00:00

  • Lack of involvement of the cholinergic mechanism in vasoactive intestinal peptide- and peptide-histidine methionine-induced growth hormone (GH) responses in acromegaly: comparison with the GH responses to thyrotropin-releasing hormone and GH-releasing hor

    abstract::We examined whether the cholinergic mechanism is involved in the paradoxical GH responses to vasoactive intestinal peptide (VIP) and peptide histidine methionine (PHM) in acromegaly. 28 patients with active acromegaly underwent i.v. bolus injections of thyrotropin-releasing hormone (TRH, 500 micrograms), GH-releasing ...


    pub_type: 临床试验,杂志文章


    authors: Watanobe H,Habu S,Nasushita R,Takebe K

    更新日期:1994-08-01 00:00:00

  • A brain peptide stimulates release of amylase from the midgut tissue of larvae of Opisina arenosella Walk. (Lepidoptera: Cryptophasidae).

    abstract::Brain extracts from 3 to 4 day old final (eighth) instar larvae of Opisina arenosella (Lepidoptera) stimulate amylase release from midgut preparations maintained in vitro. This effect of the brain extract was both time and dose dependent. The brain factor stimulating enzyme release may be a peptide as it is heat stabl...


    pub_type: 杂志文章


    authors: Harshini S,Reshmi V,Sreekumar S

    更新日期:2003-06-01 00:00:00

  • Levels of CCK immunoreactivity and dopamine in the nucleus accumbens after 6-hydroxydopamine lesions of the ventral tegmental area and the nucleus accumbens.

    abstract::The effects of 6-hydroxydopamine (6-OHDA) injected into either the ventral tegmental area (VTA) or the nucleus accumbens on dopamine and its metabolite DOPAC and CCK-immunoreactivity in the nucleus accumbens were studied. Measurements were made 1, 3 and 6 weeks after the lesion in both the anterior and posterior part ...


    pub_type: 杂志文章


    authors: Igarashi Y,Wiegant VM,Van Ree JM

    更新日期:1989-08-01 00:00:00

  • Neuropeptide Y attenuates the effect of locus coeruleus denervation by DSP-4 treatment on social behaviour in the rat.

    abstract::Noradrenaline (NA) has been implicated in both increase and reduction of anxiety. Selective destruction of nerve endings of the locus coeruleus projections by DSP-4 has been shown to reduce active behaviour in novel situations by enhancing anxiety. In the present study, DSP-4 (50 mg/kg) treatment reduced locomotor act...


    pub_type: 杂志文章


    authors: Kask A,Eller M,Oreland L,Harro J

    更新日期:2000-02-01 00:00:00

  • Calcitonin gene-related peptide (CGRP) and its role in hypertension.

    abstract::Hypertension is still presently the number one "silent killer" in the Western World, and a major risk factor for the development of secondary diseases contributing to cardiovascular disease (CVD). However, despite a broad range of therapies, the mechanisms involved in the onset of hypertension remains unclear, therefo...


    pub_type: 杂志文章,评审


    authors: Smillie SJ,Brain SD

    更新日期:2011-04-01 00:00:00

  • Specific inhibitors of pyroglutamyl peptidase I and prolyl endopeptidase do not change the in vitro release of TRH or its content in rodent brain.

    abstract::Pyroglutamyl diazomethyl ketone and N-benzyloxycarbonyl prolyl prolinal, specific inhibitors of pyroglutamyl peptidase I and prolyl endopeptidase respectively, were used to study the possible role of these enzymes in the regulation of thyrotropin releasing hormone turnover. In vitro thyrotropin releasing hormone relea...


    pub_type: 杂志文章


    authors: Charli JL,Mendez M,Joseph-Bravo P,Wilk S

    更新日期:1987-05-01 00:00:00

  • The interaction of brain insulin receptors with wheat germ agglutinin.

    abstract::Brain insulin receptors adsorb to and are recoverable from wheat germ agglutinin-agarose (WGA) columns. Similar results are obtained using dissuccinimidyl suberate (DSS)-crosslinked receptors or photo-affinity labeled receptors. WGA can be used for partial purification of brain insulin receptors provided the appropria...


    pub_type: 杂志文章


    authors: Shemer J,LeRoith D

    更新日期:1987-01-01 00:00:00

  • Oxytocin selectively increases ERalpha mRNA in the neonatal hypothalamus and hippocampus of female prairie voles.

    abstract::During neonatal development exogenous oxytocin increases ERalpha immunoreactivity in the hypothalamus of female prairie voles. The purpose of this study was to determine if the increase in ERalpha is associated with an increase in ERalpha mRNA expression and to determine if the effect is specific to ER subtype or if o...


    pub_type: 杂志文章


    authors: Pournajafi-Nazarloo H,Carr MS,Papademeteriou E,Schmidt JV,Cushing BS

    更新日期:2007-02-01 00:00:00