Modulation of CCK mRNA in cell lines in response to isoproterenol and retinoic acid.


:Regulation of cholecystokinin (CCK) expression was studied in the human neuroepithelioma cell line SK-N-MCIXC and the rat medullary thyroid carcinoma cell line WE 4/2. The cells were treated with the beta-adrenergic agonist isoproterenol and retinoic acid, a natural derivative of vitamin A, which plays a role in cell growth and proliferation. Levels of CCK mRNA were determined after 6, 12 and 24 h drug treatments, with Northern blot analysis using human CCK riboprobes. In WE 4/2 cells no differences were observed in CCK mRNA levels, between control and isoproterenol treated cells, after 6, 12 or 24 h treatments. In SK-N-MCIXC cells isoproterenol increased CCK mRNA levels at all time points examined, the beta-adrenergic antagonist propranolol blocked this effect. SK-N-MCIXC cells were also treated with actinomycin D or cycloheximide in combination with isoproterenol. Actinomycin D decreased CCK mRNA levels. Cycloheximide increased CCK mRNA levels when compared to isoproterenol acting alone. Retinoic acid did not affect CCK mRNA levels in WE 4/2 cells. In SK-N-MCIXC cells, retinoic acid consistently decreased CCK mRNA level. CCK mRNA levels in SK-N-MCIXC cells treated with retinoic acid combined with either isoproterenol or phorbol-12-myristate-13 acetate, were not significantly different from cells treated with retinoic acid alone.






Mania-Farnell BL,Botros IW,Davis TP




Has Abstract


1995-10-01 00:00:00














  • Striatal lesions and transplants demonstrate that cholecystokinin receptors are localized on intrinsic striatal neurones: a quantitative autoradiographic study.

    abstract::Considerable evidence supports the existence of modulatory interactions between cholecystokinin and dopamine in the striatum. In order to explore further the nature of such interactions, the anatomical localization of CCK receptors in rat striatum was investigated autoradiographically following selective lesions. Infu...


    pub_type: 杂志文章


    authors: Beresford IJ,Hall MD,Clark CR,Hill RG,Hughes J,Sirinathsinghji DJ

    更新日期:1987-08-01 00:00:00

  • Involvement of vasopressin and corticotropin-releasing hormone in VIP- and PHI-induced secretion of ACTH and corticosterone.

    abstract::The relative roles of hypothalamic corticotropin-releasing-hormone (CRH) and vasopressin (AVP) as mediators of the stimulant effect of vasoactive intestinal peptide (VIP) and peptide histidine isoleucine (PHI) on ACTH and corticosterone (CORT) secretion, were examined using receptor blockade of endogenous CRH and AVP....


    pub_type: 杂志文章


    authors: Alexander LD,Sander LD

    更新日期:1995-03-01 00:00:00

  • Thyroliberin (TRH) increases thymus cell proliferation in rats.

    abstract::The effects of thyroliberin (TRH), a TRH-like tripeptide colon mitosis inhibitor (CMI), a somatostatin analog SMS 201-995 and metoclopramide (dopamine receptor antagonist enhancing prolactin secretion) on rat thymus cell proliferation were investigated. The incorporation of bromodeoxyuridine (BrDU) into thymic cell nu...


    pub_type: 杂志文章


    authors: Pawlikowski M,Zerek-Mełeń G,Winczyk K

    更新日期:1992-11-01 00:00:00

  • Neuropeptide Y-like immunoreactivity in lumbar cerebrospinal fluid of patients after severe head trauma.

    abstract::We studied head-injured patients assessed after admission to the hospital according to the Glasgow coma scale (3-8 points). All of them underwent surgical procedure after neuroradiological examination. Due to therapeutic problems, some of these patients had lumbar puncture on the fourth day following surgery. The cont...


    pub_type: 杂志文章


    authors: Stachura Z,Obuchowicz E,Herman ZS

    更新日期:1997-02-01 00:00:00

  • Microinjection of CART peptide 55-102 into the nucleus accumbens blocks amphetamine-induced locomotion.

    abstract::The role played by the biologically active CART 55-102 peptide in NAcc AMPH induced locomotion was investigated. When microinjected alone into this site, none of the doses of CART 55-102 tested (0.1, 0.4, 1.0 or 2.5 microg/0.5 microl/side) produced effects on locomotion during a two-h test. However, when co-injected w...


    pub_type: 杂志文章


    authors: Kim JH,Creekmore E,Vezina P

    更新日期:2003-12-01 00:00:00

  • Acute intragastric application of capsaicin inhibits 2-deoxy-D-glucose--but not histamine-induced gastric acid secretion in the dog.

    abstract::In this study the influence of acute exposure of gastric mucosa to the sensory neurotoxin capsaicin on basal gastric acid secretion and on secretion induced by 2-deoxy-D-glucose or histamine in conscious dogs with gastric fistulae has been investigated. Under basal conditions intragastric capsaicin (160 microM, 50 ml ...


    pub_type: 杂志文章


    authors: Soldani G,Mengozzi G,Intorre L,Pacini F,Evangelista S

    更新日期:1992-12-01 00:00:00

  • The effect of C-terminus and N-terminus iodination on avian pancreatic polypeptide (APP) binding to its chicken brain receptor.

    abstract::In previous studies, membranes from chicken gastrointestinal tissues failed to bind appreciable levels of 125I-APP labeled at the C-terminus. In order to address the suggestion that this was due to steric hindrance of the critical C-terminus, N-terminally labeled 125I-APP was utilized in in vitro membrane binding assa...


    pub_type: 杂志文章


    authors: Adamo ML,Hazelwood RL

    更新日期:1992-04-01 00:00:00

  • Galanin and its receptors: introduction to the Third International Symposium, San Diego, California, USA, 21-22 October 2004.

    abstract::The Third Galanin Symposium presented many different and exciting results on galanin research reflecting a major progress since the previous symposium in 1998. A major impression was the many possible relationships of galaninergic mechanisms to important brain functions such as development, cognition and ageing as wel...




    authors: Hökfelt T

    更新日期:2005-06-01 00:00:00

  • Influence of oxytocin on nociception and morphine antinociception.

    abstract::In the hot plate test the intracerebroventricular (i.c.v.) injection of oxytocin produced a significant decrease in nociception, starting from the dose of 1 microgram/rat. A comparable effect was obtained with 10-200 times higher intraperitoneal (i.p.) doses. The i.c.v. injection of the oxytocin antagonist d(CH2)5-Tyr...


    pub_type: 杂志文章


    authors: Arletti R,Benelli A,Bertolini A

    更新日期:1993-03-01 00:00:00

  • Effect of alpha-difluoromethylornithine (DFMO) on the behavioral syndrome induced by intracerebroventricular injection of ACTH 1-24, in rats.

    abstract::Stretching-yawning syndrome (SYS) and penile erections (PE) are typical components of the behavioral picture induced by intracerebroventricular (i.c.v.) injection of ACTH 1-24. The present study shows that pretreatment with alpha-difluoromethylornithine (DFMO), and irreversible inhibitor of mammalian ornithine decarbo...


    pub_type: 杂志文章


    authors: Genedani S,Bernardi M,Bertolini A

    更新日期:1984-05-01 00:00:00

  • Dermorphin and related peptides in rat tissues.

    abstract::Dermorphin (Tyr-D-Ala-Phe-Gly-Tyr-Pro-Ser-NH2), a naturally occurring peptide isolated from arboreal frog skin, is endowed with outstanding structural and biological features. It has no structural community with the sequence of mammalian opioid peptides and is a unique example of a D-aminoacid containing peptide which...


    pub_type: 杂志文章


    authors: Mor A,Delfour A,Amiche M,Sagan S,Nicolas P,Grassi J,Pradelles P

    更新日期:1989-01-01 00:00:00

  • Prodynorphin-derived peptide expression in primate cortex and striatum.

    abstract::The distributions of four prodynorphin-derived peptides, dynorphin A (1-17), dynorphin A (1-8), dynorphin B, and alpha-neo-endorphin were determined in 10 cortical regions and the striatum of the old world monkey (Macaca nemestrina). alpha-neo-endorphin was the most abundant peptide in both cortex and striatum. The co...


    pub_type: 杂志文章


    authors: Healy DJ,Meador-Woodruff JH

    更新日期:1994-11-01 00:00:00

  • Short N-terminal galanin fragments are occurring naturally in vivo.

    abstract::The galanin family currently consists of four peptides, namely galanin, galanin-message associated peptide, galanin-like peptide and alarin. Unlike galanin that signals through three different G protein-coupled receptors; GAL1, GAL2, and GAL3, binding at its N-terminal end, the cognate receptors for other members of t...


    pub_type: 杂志文章


    authors: Ihnatko R,Theodorsson E

    更新日期:2017-06-01 00:00:00

  • Effects of intrahippocampal injection of ghrelin on spatial memory in PTZ-induced seizures in male rats.

    abstract::Ghrelin (gh) is a peptide hormone that may affect learning and memory. There is some evidence that ghrelin can have antiepileptic effects. So we decided to investigate the possible effects of ghrelin on spatial memory following PTZ-induced seizures in male rats. Ninety male rats were divided into 9 groups including co...


    pub_type: 杂志文章


    authors: Babri S,Amani M,Mohaddes G,Mirzaei F,Mahmoudi F

    更新日期:2013-10-01 00:00:00

  • Influence of typical and atypical antipsychotics on neuropeptide Y-like immunoreactivity and NPY mRNA expression in rat striatum.

    abstract::Striatal neuropeptide Y-like immunoreactivity (NPY-LI) levels were investigated in naive rats after acute, subchronic (14 days) or chronic (28 days) intraperitoneal (i.p.) treatment with chlorpromazine (2 or 10mg/kg), haloperidol (0.5 or 2 mg/kg), (+/-)sulpiride (50 or 100 mg/kg) or clozapine (10 or 25 mg/kg), and in ...


    pub_type: 杂志文章


    authors: Obuchowicz E,Turchan J

    更新日期:1998-10-01 00:00:00

  • Levels of CCK immunoreactivity and dopamine in the nucleus accumbens after 6-hydroxydopamine lesions of the ventral tegmental area and the nucleus accumbens.

    abstract::The effects of 6-hydroxydopamine (6-OHDA) injected into either the ventral tegmental area (VTA) or the nucleus accumbens on dopamine and its metabolite DOPAC and CCK-immunoreactivity in the nucleus accumbens were studied. Measurements were made 1, 3 and 6 weeks after the lesion in both the anterior and posterior part ...


    pub_type: 杂志文章


    authors: Igarashi Y,Wiegant VM,Van Ree JM

    更新日期:1989-08-01 00:00:00

  • The role of nociceptin and dynorphin in chronic pain: implications of neuro-glial interaction.

    abstract::Nociceptin-opioid peptide (NOP) receptor, also known as opioid receptor like-1 (ORL1), was identified following the cloning of the kappa-opioid peptide (KOP) receptor, and the characterization of these receptors revealed high homology. The endogenous ligand of NOP, nociceptin (NOC), which shares high homology to dynor...


    pub_type: 杂志文章,评审


    authors: Mika J,Obara I,Przewlocka B

    更新日期:2011-08-01 00:00:00

  • Evidence for the involvement of an opioid system in sciatic nerve of Rana ridibunda.

    abstract::The effect of opioid peptide, D-alanine2-leucine-enkephalin and opioid homolog peptide, des-tyrosine-methionine-enkephalin in concentrations of 1 x 10(-6) and 1 x 10(-5) M was investigated on the action potential parameters of frog sciatic nerve. Des-tyrosine-methionine-enkephalin was used as the control to prove the ...


    pub_type: 杂志文章


    authors: Camlica Y,Aşkin A,Cömelekoğlu U

    更新日期:2004-04-01 00:00:00

  • Some projections of dynorphin-immunoreactive neurons in the rat central nervous system.

    abstract::Dynorphin 1-8 (DYN 1-8) and Dynorphin B (DYN B) have been immunocytochemically localized to neuronal perikarya throughout the adult rat central nervous system (CNS). In order to determine if any DYN 1-8 and DYN B immunoreactive (DYN-I) cell bodies have long projections, indirect immunofluorescence was used in combinat...


    pub_type: 杂志文章


    authors: Code RA,Fallon JH

    更新日期:1986-08-01 00:00:00

  • The gamma(2)-MSH peptide mediates a central analgesic effect via a GABA-ergic mechanism that is independent from activation of melanocortin receptors.

    abstract::Using the latency for tail-flick after thermal stimulation we have assessed the effects of alpha-, gamma(1)- and gamma(2)-MSH on nociceptive threshold in the mice. Intracisternal injections of gamma(2)-MSH induced a distinct analgesia, while gamma(1)-MSH in the same doses gave only a minor analgesia. Intracisternal al...


    pub_type: 杂志文章


    authors: Klusa V,Germane S,Svirskis S,Opmane B,Wikberg JE

    更新日期:2001-02-01 00:00:00

  • Association study of two functional single nucleotide polymorphisms of neuropeptide y gene with multiple sclerosis.

    abstract::Multiple sclerosis (MS) is an autoimmune disease of the central nervous system characterized by brain inflammation, demyelination and axonal loss. Neuropeptide Y (NPY) has a critical role in the maintenance of homeostasis in the immune system and coping of stress condition. In the current study we analyzed 188 patient...


    pub_type: 杂志文章


    authors: Mohammadi SM,Shirvani Farsani Z,Dosti R,Sahraian MA,Behmanesh M

    更新日期:2016-12-01 00:00:00

  • Peripheral neurotensin participates in the modulation of pre- and postprandial intestinal motility in rats.

    abstract::The present study was undertaken to determine whether neurotensin is involved in the regulation of the intestinal postprandial motor response and, if so, whether the regulatory pathway depends upon peripheral or central neurotensin secretion. Neurotensin, injected by the i.v. route (5 micrograms/kg) during the fasting...


    pub_type: 杂志文章


    authors: Pellissier S,Eribon O,Chabert J,Gully D,Roche M

    更新日期:1996-10-01 00:00:00

  • Neural and non-neural origin of calcitonin gene-related peptide (CGRP) in the gastric mucosa.

    abstract::We have used immunohistochemistry and in situ hybridization histochemistry to visualize CGRP and the mRNA encoding the CGRP precursor in the stomach. CGRP is present in nerve fibers in the mucosa. CGRP mRNA and CGRP itself are also found in non-neural cells in the lamina propria. These cells are likely to be macrophag...


    pub_type: 杂志文章


    authors: Jakab G,Webster HD,Salamon I,Mezey E

    更新日期:1993-02-01 00:00:00

  • Influences of calcitonin gene-related peptide on mu opioid receptors in nucleus accumbens neurons of rats.

    abstract::The Mu opioid receptor (MOR) has been shown to participate in the analgesic effect of the calcitonin gene-related peptide (CGRP) in the nucleus accumbens (NAc) of adult rats. However, it is not clear whether and how CGRP regulates the MOR at the molecular levels. In the present study, it is found that the level of MOR...


    pub_type: 杂志文章


    authors: Yan H,Yu LC

    更新日期:2013-04-01 00:00:00

  • Changes in thymus size, cellularity and relation between thymocyte subpopulations in young adult rats induced by somatostatin-14.

    abstract::The role of somatostatin on inhibition of both normal and tumor cell cycle, secretion of endocrine and exocrine cells, as well as induction apoptosis is well documented. However, its effect on T cell development and thymic structure is not fully clarified. In order to investigate the influence of somatostatin in vivo ...


    pub_type: 杂志文章


    authors: Petrović-Dergović DM,Rakin AK,Dimitrijević LA,Ristovski JS,Kustrimović NZ,Mićić MV

    更新日期:2007-12-01 00:00:00

  • Somatostatin binding to a fresh rat astrocyte-enriched suspension.

    abstract::Since there are conflicting reports regarding the effects of somatostatin (SS) on cyclic AMP levels in astrocytes derived from rat cerebral cortex and, to date, the SS binding to mature astrocytes is unknown, the present study has determined SS binding and its effect on cyclic AMP accumulation in a fresh astrocyte-ric...


    pub_type: 杂志文章


    authors: Colás B,Arilla E,Prieto JP

    更新日期:1992-09-01 00:00:00

  • Neuropeptide Y associated with asthma in young adults.

    abstract:OBJECTIVE:Neuropeptide Y, a widely circulating neurotransmitter, plays a pivotal role in energy balance, immunomodulation and asthma, and several NPY polymorphisms are promising genetic risk factors for asthma and obesity. We explored the associations of candidate NPY gene polymorphisms with prevalent asthma and its re...


    pub_type: 杂志文章


    authors: Lu Y,Andiappan AK,Lee B,Ho R,Lim TK,Kuan WS,Goh DY,Mahadevan M,Sim TB,Wang Y,Van Bever HP,Rotzschke O,Larbi A,Ng TP

    更新日期:2016-10-01 00:00:00

  • Changes in pituitary adenylate cyclase activating peptide and vasoactive intestinal peptide innervation of rat oxyntic mucosa during ulcer healing.

    abstract::Pituitary adenylate cyclase activating peptide and vasoactive intestinal peptide belong to the same neuropeptide family. Both peptides are present in nerve fibers in the gastric wall and are thought to be involved in the regulation of inflammatory processes. Experimental ulcers were induced in the rat gastric mucosa b...


    pub_type: 杂志文章


    authors: Mei Q,Sundler F

    更新日期:1998-12-01 00:00:00

  • Central and peripheral type benzodiazepine ligands displace [3H][3-ME-HIS2]TRH from its binding sites in the brain and the anterior pituitary and antagonize the effect of TRH in the rat duodenum.

    abstract::The effects of central (clonazepam, an agonist, and FG 7142, an inverse agonist), mixed (diazepam) or peripheral type (Ro 5-4864) benzodiazepine receptor ligands on the action of TRH on the transmurally stimulated rat duodenum and binding of [3H][3-Me-His2] TRH in the rat anterior pituitary, hypothalamus, cortex and b...


    pub_type: 杂志文章


    authors: Järvinen A,Paakkari I,Männistö PT

    更新日期:1991-07-01 00:00:00

  • Cardioacceleration provoked by intrathecal administration of vasoactive intestinal peptide (VIP): mediation by a non-central nervous system mechanism.

    abstract::Intrathecal administration of VIP to the thoracic spinal cord in the urethane anaesthetized rat provoked a dose-dependent increase in heart rate without any change in arterial pressure. The cardioacceleration observed following administration of 6.5 nmol of VIP at the T9 level (n = 8) occurred within 1-2 min of admini...


    pub_type: 杂志文章


    authors: Rochford J,Henry JL

    更新日期:1989-11-01 00:00:00