Abstract：
:Regulation of cholecystokinin (CCK) expression was studied in the human neuroepithelioma cell line SK-N-MCIXC and the rat medullary thyroid carcinoma cell line WE 4/2. The cells were treated with the beta-adrenergic agonist isoproterenol and retinoic acid, a natural derivative of vitamin A, which plays a role in cell growth and proliferation. Levels of CCK mRNA were determined after 6, 12 and 24 h drug treatments, with Northern blot analysis using human CCK riboprobes. In WE 4/2 cells no differences were observed in CCK mRNA levels, between control and isoproterenol treated cells, after 6, 12 or 24 h treatments. In SK-N-MCIXC cells isoproterenol increased CCK mRNA levels at all time points examined, the beta-adrenergic antagonist propranolol blocked this effect. SK-N-MCIXC cells were also treated with actinomycin D or cycloheximide in combination with isoproterenol. Actinomycin D decreased CCK mRNA levels. Cycloheximide increased CCK mRNA levels when compared to isoproterenol acting alone. Retinoic acid did not affect CCK mRNA levels in WE 4/2 cells. In SK-N-MCIXC cells, retinoic acid consistently decreased CCK mRNA level. CCK mRNA levels in SK-N-MCIXC cells treated with retinoic acid combined with either isoproterenol or phorbol-12-myristate-13 acetate, were not significantly different from cells treated with retinoic acid alone.
journal_name
Neuropeptidesjournal_title
Neuropeptidesauthors
Mania-Farnell BL,Botros IW,Davis TPdoi
10.1016/0143-4179(95)90064-0subject
Has Abstractpub_date
1995-10-01 00:00:00pages
221-7issue
4eissn
0143-4179issn
1532-2785pii
0143-4179(95)90064-0journal_volume
29pub_type
杂志文章相关文献
NEUROPEPTIDES文献大全abstract：:Ghrelin (gh) is a peptide hormone that may affect learning and memory. There is some evidence that ghrelin can have antiepileptic effects. So we decided to investigate the possible effects of ghrelin on spatial memory following PTZ-induced seizures in male rats. Ninety male rats were divided into 9 groups including co...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2013.05.005
更新日期：2013-10-01 00:00:00
abstract：:Using field potential recording in the CA1 region of hippocampal slices from rats injected with cysteamine (200 mg/kg, s.c.), changes in activity and plasticity of Schaffer collateral-CA1 pyramidal cell synapses were examined. Extracellular field potential recording prior to and following either theta-pattern primed b...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/s0143-4179(02)00029-x
更新日期：2002-08-01 00:00:00
abstract：:There is now considerable evidence that putative neuropeptides are involved in epileptogenic processes. Neurotensin (NT), which affects neuronal excitability in many parts of the CNS, is potentially relevant to the kindling phenomenon, but has not previously been studied in the kindling model of epilepsy. This study c...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(88)90014-5
更新日期：1988-02-01 00:00:00
abstract：:After chronic treatment with morphine, the muscular rigidity mediated by opioid receptors in the striatum, was gradually replaced by stereotypic behaviour indicating an increased nigrostriatal dopaminergic activity. These results suggest that one behavioural pattern, originating in the striatum, was replaced by anothe...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(84)90018-0
更新日期：1984-12-01 00:00:00
abstract：:In critical patients, sepsis-induced muscle wasting is considered to be an important contributor to complications and mortality. Previous work mainly focuses on the peripheral molecular mechanism of muscle degradation, however little evidence exists for the role of central nervous system in the process. In the present...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2014.10.002
更新日期：2014-12-01 00:00:00
abstract：:Brain insulin receptors adsorb to and are recoverable from wheat germ agglutinin-agarose (WGA) columns. Similar results are obtained using dissuccinimidyl suberate (DSS)-crosslinked receptors or photo-affinity labeled receptors. WGA can be used for partial purification of brain insulin receptors provided the appropria...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(87)90025-4
更新日期：1987-01-01 00:00:00
abstract：:The role of somatostatin and a stable analogue, sandostatin (Octreotide), on the responses of spinal cord neurones in vivo was investigated in the rat. Electrical C-fibre stimulation was used as a model of acute nociception and the response to subcutaneous formalin was used as a model of longer term events. Intratheca...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(92)90115-d
更新日期：1992-11-01 00:00:00
abstract：:This study investigated the nature of the cholecystokinin-like immunoreactivity (CCKLI) in the dorsal horn of the rat spinal cord. Indeed, using the same antiserum, no variation of radioimmunoassayable CCKLI in the dorsal horn of rats treated neonatally with capsaicin could be seen, whereas the density of immunoreacti...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(90)90033-u
更新日期：1990-12-01 00:00:00
abstract：:The tetra-peptide FMRF-NH(2) is a cardioexcitatory peptide in the clam. Using the antibody against this peptide, FMRF-NH(2)-like immunoreactive material was detected in mammalian CNS. Subsequently, mammalian FMRF-NH(2) immunoreactive peptides were isolated from bovine brain and characterized to be FLFQPQRF-NH(2) (NPFF...
journal_title：Neuropeptides
pub_type： 杂志文章,评审
doi：10.1016/j.npep.2007.06.004
更新日期：2008-02-01 00:00:00
abstract：:Chronic stress induces the activation of hippocampal microglia, which produces many inflammatory mediators and mediates the occurrence of depression. Two phenotypes of microglia polarization, the classical M1 and alternative M2, play important regulatory roles in neuroinflammation and are involved in the occurrence an...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2020.102006
更新日期：2020-06-01 00:00:00
abstract：:We investigated the effect of sulphated cholecystokinin octapeptide (CCK-8S) on free intracellular calcium concentration, phosphatidylinositol metabolism, and protein phosphorylation in C6 cells, a rat glioma cell line which was shown to express CCKB type receptors. Increase in [Ca2+]i by both influx across the cell m...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(95)90033-0
更新日期：1995-11-01 00:00:00
abstract：:Intrathecal administration of VIP to the thoracic spinal cord in the urethane anaesthetized rat provoked a dose-dependent increase in heart rate without any change in arterial pressure. The cardioacceleration observed following administration of 6.5 nmol of VIP at the T9 level (n = 8) occurred within 1-2 min of admini...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(89)90053-x
更新日期：1989-11-01 00:00:00
abstract：:Acid extracts from mouse Neuroblastoma x rat Glioma hybrid cells have been purified by means of Sep-Pak C-18 and fractionated by high performance liquid chromatography. Each fraction has been submitted to a sensitive beta-endorphin radioimmunoassay and an immunoreactivity peak at camel beta-endorphin retention time wa...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(85)90130-1
更新日期：1985-03-01 00:00:00
abstract：:The effects of graded amounts of centrally injected [intracerebroventricular, i.c.v.] oxytocin [OXT] have been studied on the steady-state level and pargyline-induced accumulation of serotonin [5-HT] in the hypothalamus, mesencephalon and dorsal hippocampus of rats. OXT only weakly affected the steady-state level of 5...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(84)90125-2
更新日期：1984-03-01 00:00:00
abstract：:3H-Bradykinin binding sites with the characteristics of receptors were identified in homogenates of bovine hippocampus and PC-12 cells. The characteristics of the binding were similar in both types of tissue and paralleled those of the B2 bradykinin receptor. Following exposure to nerve growth factor for 72 hours, the...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(88)90056-x
更新日期：1988-11-01 00:00:00
abstract：:We have previously shown that one of the major determinants directing the expression of the preprotachykinin-A (TAC1) gene, which encodes the neuropeptide substance P, is the transcription factor Neuronal Restrictive Silencer Factor (NSRF), which is also termed Repressor Element-1 Silencing Factor (REST). In rodent mo...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2009.05.004
更新日期：2009-08-01 00:00:00
abstract：:Male Wistar rats were injected intraperitoneally with chlorpromazine (2 or 10 mg/kg), haloperidol (0.5 or 2 mg/kg), sulpiride (50 or 100 mg/kg) or clozapine (10 or 25 mg/kg) once, for 14 or 28 consecutive days. Hypothalamic neuropeptide Y-like (NPY-like) immunoreactivity (NPY-LI) was determined 24 h after the last dos...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/s0143-4179(96)90012-8
更新日期：1996-10-01 00:00:00
abstract：:In order to study the morphological localization of the endogenous benzodiazepine ligand octadecaneuropeptide (ODN) in rat brain, we have developed antibodies against this peptide. Using a radioimmunoassay for ODN, we have observed that synthetic ODN and serial dilutions of several brain areas gave parallel displaceme...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(90)90155-r
更新日期：1990-01-01 00:00:00
abstract：:To study the effect of septicaemia, the temporal changes in tissue adrenomedullin (AM) and preproAM mRNA levels were studied in the heart and blood vessels after lipopolysaccharide (LPS) injection. Radioimmunoassay and solution hybridization-RNase protection assays were used to follow the changes in AM and its mRNA le...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2004.11.002
更新日期：2005-04-01 00:00:00
abstract：:The effects of acute and chronic treatment with LSD and acute treatment with mescaline, m-CPP, citalopram and fenfluramine on leu-enkephalin (LENK) content in rat brain were examined. The acute LSD treatment was found to decrease and chronic treatment to increase the LENK content in the frontal cortex. In the thalamus...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(86)90028-4
更新日期：1986-05-01 00:00:00
abstract：:HPLC techniques were used to identify peptides that possess vasopressin-like immunoreactivity in the chicken neurohypophysis. The presence of arginine vasotocin (AVT) was confirmed together with arginine vasopressin (AVP). That the presence of AVP may be confined to the chicken, and not other avian species, was conclu...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(86)90045-4
更新日期：1986-08-01 00:00:00
abstract：:We have recently documented the expression of preprothyrotropin-releasing hormone (TRH) gene in murine, human and rat testis. Moreover, we have localized TRH to rat Leydig cells immunohistochemically, and found that both prepro TRH mRNA and TRH levels are developmentally regulated in the rat testis. To investigate the...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(94)90070-1
更新日期：1994-09-01 00:00:00
abstract：:Orexins are neuropeptides that are localized to neurons in the lateral and dorsal hypothalamus but its receptors are distributed to many different regions of the central nervous system. Orexins are implicated in a variety of physiological functions including sleep regulation, energy homeostats, and stress reactions. F...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2016.12.007
更新日期：2017-02-01 00:00:00
abstract：:To find out, whether microheterogeneity of neurophysins is due to aging of neurosecretory granules we investigated neurophysin proteins of porcine origin from newly formed and aged granules, as well as from two different extraction methods from whole posterior pituitary glands by high performance liquid chromatography...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(83)90003-3
更新日期：1983-12-01 00:00:00
abstract：:In our previous study we have identified PACAP, VIP and their receptors in rat malignant peripheral nerve sheath tumor (MPNST) cells, thus showing anti-apoptotic roles. Recently it has been shown that the tumor suppressor neurofibromin, encoded by the Neurofibromatosis type I (NF1) gene, promotes MPNST cells sensitivi...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2009.10.003
更新日期：2010-02-01 00:00:00
abstract：:Neuropeptide Y (NPY) is known to bind to at least two types of receptors (Y1 & Y2). One type (Y2) is able to bind and undergo activation by both NPY and its C-terminal fragments with good potency while the other (Y1) requires the full length of NPY for good potency. For most NPY analogs that have been examined, potenc...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(90)90073-8
更新日期：1990-11-01 00:00:00
abstract：:Kyotorphin (KTP), first isolated in the bovine brain and now having been identified in a variety of species, is known most extensively for its analgesic-like properties. KTP indirectly stimulates opioid receptors by releasing methionine enkephalin (met-enkephalin). Stimulation of opioid receptors is linked to hunger p...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2012.10.007
更新日期：2013-06-01 00:00:00
abstract：:In rat preprogalanin, galanin is C-terminally flanked by a 60 amino acid long peptide: galanin message-associated peptide (GMAP). GMAP sequences in different species show high degree of homology, suggesting a biological role. However, the study of the physiological and pharmacological actions of this peptide have been...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/s0143-4179(98)90037-3
更新日期：1998-04-01 00:00:00
abstract：:Cholecystokinin (CCK) is a widely expressed neuropeptide system originally discovered in the gut. Both cerebral and peripheral neurons as well as endocrine I-cells in the small intestine process proCCK to tyrosyl-O-sulfated and α-carboxyamidated peptides. Recently, we reported that gut endocrine I-cells also synthetiz...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2016.08.003
更新日期：2016-12-01 00:00:00
abstract：:Several lines of evidence indicate a role for neuropeptide Y (NPY) in the modulation of the corticotroph axis. In two separate studies reported here, the concentrations of NPY and noradrenaline (NA), as well as corticotropin-releasing factor (CRF) and arginine vasopressin (AVP), were measured in extracts of individual...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(93)90107-l
更新日期：1993-10-01 00:00:00
