Cholecystokinin induced signaling in rat glioma C6 cells.


:We investigated the effect of sulphated cholecystokinin octapeptide (CCK-8S) on free intracellular calcium concentration, phosphatidylinositol metabolism, and protein phosphorylation in C6 cells, a rat glioma cell line which was shown to express CCKB type receptors. Increase in [Ca2+]i by both influx across the cell membrane and release from internal stores was demonstrated by utilizing a laser confocal imaging system. Because CCK-8S produced a transient elevation of inositol triphosphate level participation of InsP3 in calcium signaling in C6 cells is very likely. Protein kinase C seems to be involved in CCK-8S induced signaling in rat glioma C6 cells as demonstrated by using in vivo phosphorylation experiments.






Kaufmann R,Schöneberg T,Lindschau C,Haller H,Ott T




Has Abstract


1995-11-01 00:00:00














  • Age-related impairments of synaptic plasticity in the lateral perforant path input to the dentate gyrus of galanin overexpressing mice.

    abstract::In the present study, electrophysiological recordings were made from hippocampal slices obtained from mice overexpressing galanin under the promoter for the platelet-derived growth factor-B (GalOE mice). In these mice, a particularly strong galanin expression is seen in the granule cell layer/mossy fibers. Paired-puls...


    pub_type: 杂志文章


    authors: Zheng K,Kuteeva E,Xia S,Bartfai T,Hökfelt T,Xu ZQ

    更新日期:2005-06-01 00:00:00

  • Tritiated-6-beta-fluoro-6-desoxy-oxymorphone ([3H]FOXY): a new ligand and photoaffinity probe for the mu opioid receptors.

    abstract::6-Beta-fluoro-6-desoxy-oxymorphone (FOXY) is a fluorinated derivative of oxymorphone originally developed as a potential PET scanning ligand. Preliminary work (Rothman et al., Neuropeptides 4: 311-317, 1984) demonstrated that [3H]FOXY selectively labeled mu opioid binding sites with low levels of nonspecific binding. ...


    pub_type: 杂志文章


    authors: Rothman RB,Bykov V,Rice KC,Jacobson AE,Kooper GN,Bowen WD

    更新日期:1988-01-01 00:00:00

  • Alarin stimulates food intake in male rats and LH secretion in castrated male rats.

    abstract::Alarin is a newly identified member of the galanin family of neuropeptides that includes galanin-like peptide (GALP) and galanin. Alarin was discovered as an alternate transcript of the GALP gene in neuroblastoma cells, and subsequently alarin mRNA was detected in the brain of rodents. GALP and galanin are important c...


    pub_type: 杂志文章


    authors: Van Der Kolk N,Madison FN,Mohr M,Eberhard N,Kofler B,Fraley GS

    更新日期:2010-08-01 00:00:00

  • The effects of corticotropin-releasing hormone on peptidergic neurons in the rat forebrain.

    abstract::Our previous study showed that intracerebroventricular (ICV) administration of corticotropin-releasing hormone (CRH) produced a significant increase in locomotor activity at a dose of 1 microgram and slow stereotypy with prominent grooming at a dose of 10 micrograms. In addition, the ICV administration of CRH caused a...


    pub_type: 杂志文章


    authors: Takamatsu Y,Yamamoto H,Ogunremi OO,Matsuzaki I,Moroji T

    更新日期:1991-12-01 00:00:00

  • A putative morphological substrate of the catecholamine-influenced neuropeptide Y (NPY) release in the human hypothalamus.

    abstract::Neuropeptide Y (NPY) is a 36 amino acid peptide, which among others, plays a pivotal role in stress response. Although previous studies confirmed that NPY release is increased by stress in several species, the exact mechanism of the stress-induced NPY release has not been elucidated yet. In the present study, we exami...


    pub_type: 杂志文章


    authors: Ko L,Rotoli G,Grignol G,Hu W,Merchenthaler I,Dudas B

    更新日期:2011-06-01 00:00:00

  • Effects of vasoactive intestinal peptide (VIP) on scopolamine-induced amnesia in the rat.

    abstract::The effects of vasoactive intestinal peptide (VIP) on spatial cognitive deficits induced in the rat by injections of scopolamine were examined in a radial arm maze. A single intraperitoneal (i.p.), subcutaneous (s.c.) or intracerebroventricular (i.c.v.) injection of VIP inhibited the reduction in the number of initial...


    pub_type: 杂志文章


    authors: Yamaguchi Y,Kobayashi H

    更新日期:1994-03-01 00:00:00

  • Investigation of receptor binding and functional characteristics of hemopressin(1-7).

    abstract::The orally active, α-hemoglobin derived hemopressin (PVNFKFLSH, Hp(1-9)) and its truncated (PVNFKFL, Hp(1-7) and PVNFKF, Hp(1-6)) and extended ((R)VDPVNFKFLSH, VD-Hp(1-9) and RVD-Hp(1-9)) derivatives have been postulated to be the endogenous peptide ligands of the cannabinoid receptor type 1 (CB1). In an attempt to cr...


    pub_type: 杂志文章


    authors: Dvorácskó S,Tömböly C,Berkecz R,Keresztes A

    更新日期:2016-08-01 00:00:00

  • Association study of two functional single nucleotide polymorphisms of neuropeptide y gene with multiple sclerosis.

    abstract::Multiple sclerosis (MS) is an autoimmune disease of the central nervous system characterized by brain inflammation, demyelination and axonal loss. Neuropeptide Y (NPY) has a critical role in the maintenance of homeostasis in the immune system and coping of stress condition. In the current study we analyzed 188 patient...


    pub_type: 杂志文章


    authors: Mohammadi SM,Shirvani Farsani Z,Dosti R,Sahraian MA,Behmanesh M

    更新日期:2016-12-01 00:00:00

  • Galanin induced in sympathetic neurons after axotomy is anterogradely transported toward regenerating nerve endings.

    abstract::Peripheral neurons begin to express galanin after axotomy. When neurons in the superior cervical ganglion were axotomized near (about 2 mm) from the ganglion, galanin-like immunoreactivity (IR) was maximal within 72 h. Axotomy of neurons in the middle and inferior cervical ganglion complex (MICG), which could be perfo...


    pub_type: 杂志文章


    authors: Shadiack AM,Zigmond RE

    更新日期:1998-06-01 00:00:00

  • BDNF expression with functional improvement in transected spinal cord treated with neural stem cells in adult rats.

    abstract::Neural stem cells (NSC) could promote the repair after spinal cord transection (SCT), the underlying mechanism, however, still keeps to be defined. This study reported that NSC grafts significantly improved sensory and locomotor functions in adult rats with SCT in acute stage after injury. NSC could survive; different...


    pub_type: 杂志文章


    authors: He BL,Ba YC,Wang XY,Liu SJ,Liu GD,Ou S,Gu YL,Pan XH,Wang TH

    更新日期:2013-02-01 00:00:00

  • Buspirone and related compounds as alternative anxiolytics.

    abstract::The desire of the pharmaceutical industry to obtain more selective agents for the treatment of anxiety with fewer or diminished side effects and a profile consistent with safety during long-term use resulted in a search which has identified the azapirones as a new class of anxiolytics which lack structural or biochemi...


    pub_type: 临床试验,杂志文章,评审


    authors: Taylor DP,Moon SL

    更新日期:1991-07-01 00:00:00

  • Long-term treatment with chlorpromazine and haloperidol but not with sulpiride and clozapine markedly elevates neuropeptide Y-like immunoreactivity in the rat hypothalamus.

    abstract::Male Wistar rats were injected intraperitoneally with chlorpromazine (2 or 10 mg/kg), haloperidol (0.5 or 2 mg/kg), sulpiride (50 or 100 mg/kg) or clozapine (10 or 25 mg/kg) once, for 14 or 28 consecutive days. Hypothalamic neuropeptide Y-like (NPY-like) immunoreactivity (NPY-LI) was determined 24 h after the last dos...


    pub_type: 杂志文章


    authors: Obuchowicz E

    更新日期:1996-10-01 00:00:00

  • Structure-activity studies of C- and N-terminal fragments of cholecystokinin 26-33 in guinea pig isolated tissues.

    abstract::In vitro structure-activity studies with cholecystokinin (CCK)/gastrin-related peptides, including C- and N-terminal fragments of CCK 26-33, were undertaken in guinea pig gallbladder and ileum. The general order of potency in both smooth muscle preparations is CCK 26-33 greater than CCK 1-33 greater than 27-33 much mu...


    pub_type: 杂志文章


    authors: Gaudreau P,St-Pierre S,Pert CB,Quirion R

    更新日期:1987-07-01 00:00:00

  • Effects of the peripherally selective kappa-opioid asimadoline, on substance P and CGRP mRNA expression in chronic arthritis of the rat.

    abstract::We have previously shown that the kappa-opioid agonist, asimadoline, produces time-dependent changes in neuropeptide concentrations in the joints of rats with chronic arthritis. We hypothesized that asimadoline acts on peripheral terminals to modulate substance P (SP) release. To address this hypothesis, here we have ...


    pub_type: 杂志文章


    authors: Walker JS,Scott C,Bush KA,Kirkham BW

    更新日期:2000-06-01 00:00:00

  • Embryonic ablation of neuronal VGF increases energy expenditure and reduces body weight.

    abstract::Germline ablation of VGF, a secreted neuronal, neuroendocrine, and endocrine peptide precursor, results in lean, hypermetabolic, and infertile adult mice that are resistant to diet-, lesion-, and genetically-induced obesity and diabetes (Hahm et al., 1999, 2002). To assess whether this phenotype is predominantly drive...


    pub_type: 杂志文章


    authors: Jiang C,Lin WJ,Sadahiro M,Shin AC,Buettner C,Salton SR

    更新日期:2017-08-01 00:00:00

  • Thyroliberin (TRH) increases thymus cell proliferation in rats.

    abstract::The effects of thyroliberin (TRH), a TRH-like tripeptide colon mitosis inhibitor (CMI), a somatostatin analog SMS 201-995 and metoclopramide (dopamine receptor antagonist enhancing prolactin secretion) on rat thymus cell proliferation were investigated. The incorporation of bromodeoxyuridine (BrDU) into thymic cell nu...


    pub_type: 杂志文章


    authors: Pawlikowski M,Zerek-Mełeń G,Winczyk K

    更新日期:1992-11-01 00:00:00

  • Differential effects of forskolin and phorbol-13-myristate injected intrathecally or intracerebroventricularly on antinociception induced by morphine or beta-endorphin administered intracerebroventricularly in the mouse.

    abstract::The effects of forskolin or phorbol-13-myristate (PMA) injected intrathecally (i.t.) or intracerebroventricularly (i.c.v.) on the inhibition of the tail-flick and hotplate responses induced by morphine or beta-endorphin administered i.c.v. were studied. Animals pretreated with forskolin (20 micrograms) i.t. for 10 min...


    pub_type: 杂志文章


    authors: Suh HW,Song DK,Sim YB,Chung KM,Kim YH

    更新日期:1996-04-01 00:00:00

  • CART expression in limbic regions of rat brain following forced swim stress: sex differences.

    abstract::Our previous studies showed the modulation of cocaine and amphetamine regulated transcript (CART) positive neurons and CART mRNA by adrenalectomy and corticosterone replacement in hypothalamic nuclei of male rat brain. More recently, we have shown by CART immunohistochemistry that restraint and forced swim (FS) stress...


    pub_type: 杂志文章


    authors: Balkan B,Gozen O,Yararbas G,Koylu EO,Akinturk S,Kuhar MJ,Pogun S

    更新日期:2006-06-01 00:00:00

  • Neural and non-neural origin of calcitonin gene-related peptide (CGRP) in the gastric mucosa.

    abstract::We have used immunohistochemistry and in situ hybridization histochemistry to visualize CGRP and the mRNA encoding the CGRP precursor in the stomach. CGRP is present in nerve fibers in the mucosa. CGRP mRNA and CGRP itself are also found in non-neural cells in the lamina propria. These cells are likely to be macrophag...


    pub_type: 杂志文章


    authors: Jakab G,Webster HD,Salamon I,Mezey E

    更新日期:1993-02-01 00:00:00

  • The effects of chronic glucocorticoid excess, adrenalectomy and stress on neuropeptide Y in individual rat hypothalamic nuclei.

    abstract::Several lines of evidence indicate a role for neuropeptide Y (NPY) in the modulation of the corticotroph axis. In two separate studies reported here, the concentrations of NPY and noradrenaline (NA), as well as corticotropin-releasing factor (CRF) and arginine vasopressin (AVP), were measured in extracts of individual...


    pub_type: 杂志文章


    authors: Pralong FP,Corder R,Gaillard RC

    更新日期:1993-10-01 00:00:00

  • Characterization of NK-3 binding sites in rat and guinea pig cortical membranes by the selective ligand [3H]Senktide.

    abstract::We have used [3H]Senktide, a selective Neurokinin B receptor ligand, for the characterization of NK-3 receptors in rat and guinea pig CNS membranes. Scatchard analysis of saturation binding studies in cerebral cortex membranes indicated that this ligand bound to a single site with apparent high affinity (KD = 4.6 +/- ...


    pub_type: 杂志文章


    authors: Renzetti AR,Barsacchi P,Criscuoli M,Lucacchini A

    更新日期:1991-03-01 00:00:00

  • Effect of chronic morphine administration on measured levels and chromatographic characterization of six neuropeptides in guinea-pig ileum.

    abstract::Guinea-pigs were chronically treated with morphine by subcutaneous implantation of a compounded morphine pellet. After 7 days, the animals were sacrificed, and the small intestine was removed and extracted for the assay of 6 neuropeptides (substance P, vasoactive intestinal peptide, galanin, dynorphin A (1-8), dynorph...


    pub_type: 杂志文章


    authors: Shuttleworth CW,Murphy R

    更新日期:1988-11-01 00:00:00

  • The patterns of exercise-induced β-endorphin expression in the central nervous system of rats.

    abstract::Exercise at different intensities is able to induce different physical and psychological statuses of the subjects. The β-endorphin (β-EP) in central nervous system is thought to play an important role in physical exercise. However, its expression patterns and physiological effects in the central nuclei under different...


    pub_type: 杂志文章


    authors: Xue L,Sun J,Zhu J,Ding Y,Chen S,Ding M,Pei H

    更新日期:2020-08-01 00:00:00

  • Modulating Aβ Fibrillogenesis with 'Trojan' peptides.

    abstract::Abnormal aggregation of beta-amyloid (Aβ) peptide into amyloid plaques in the brain has been identified as one of the key factors in instigating AD pathogenesis. Inhibition of Aβ aggregation can be an important therapeutic strategy in disease management. In this work, we demonstrate the application of structure-based ...


    pub_type: 杂志文章


    authors: Pandey G,Morla S,Kumar S,Ramakrishnan V

    更新日期:2020-06-01 00:00:00

  • Characterization of human placental opioid receptors by 3H-ethylketocyclazocine and 3H-naloxone binding.

    abstract::The human placenta contains membrane bound opioid receptors. The binding of the antagonist naloxone and the fairly kappa-selective agonist EKC were studied in the microvillous membrane fraction. In both cases high affinity binding sites were detected with Kd values in the nanomolar range. A series of kappa-selective l...


    pub_type: 杂志文章


    authors: Agbas A,Simon J,Avni Oktem H,Varga E,Borsodi A

    更新日期:1988-10-01 00:00:00

  • Microinjection of CART peptide 55-102 into the nucleus accumbens blocks amphetamine-induced locomotion.

    abstract::The role played by the biologically active CART 55-102 peptide in NAcc AMPH induced locomotion was investigated. When microinjected alone into this site, none of the doses of CART 55-102 tested (0.1, 0.4, 1.0 or 2.5 microg/0.5 microl/side) produced effects on locomotion during a two-h test. However, when co-injected w...


    pub_type: 杂志文章


    authors: Kim JH,Creekmore E,Vezina P

    更新日期:2003-12-01 00:00:00

  • The ability of hippocampal CA1 area for induction of long-term potentiation is persistently reduced by prior treatment with cysteamine: an in vitro study.

    abstract::Using field potential recording in the CA1 region of hippocampal slices from rats injected with cysteamine (200 mg/kg, s.c.), changes in activity and plasticity of Schaffer collateral-CA1 pyramidal cell synapses were examined. Extracellular field potential recording prior to and following either theta-pattern primed b...


    pub_type: 杂志文章


    authors: Rostampour M,Fathollahi Y,Semnanian S,Hajizadeh S,Mirnajafizadeh J,Shafizadeh M

    更新日期:2002-08-01 00:00:00

  • The gamma(2)-MSH peptide mediates a central analgesic effect via a GABA-ergic mechanism that is independent from activation of melanocortin receptors.

    abstract::Using the latency for tail-flick after thermal stimulation we have assessed the effects of alpha-, gamma(1)- and gamma(2)-MSH on nociceptive threshold in the mice. Intracisternal injections of gamma(2)-MSH induced a distinct analgesia, while gamma(1)-MSH in the same doses gave only a minor analgesia. Intracisternal al...


    pub_type: 杂志文章


    authors: Klusa V,Germane S,Svirskis S,Opmane B,Wikberg JE

    更新日期:2001-02-01 00:00:00

  • The influence of oxytocin on the steady-state level and accumulation of serotonin in rat brain regions.

    abstract::The effects of graded amounts of centrally injected [intracerebroventricular, i.c.v.] oxytocin [OXT] have been studied on the steady-state level and pargyline-induced accumulation of serotonin [5-HT] in the hypothalamus, mesencephalon and dorsal hippocampus of rats. OXT only weakly affected the steady-state level of 5...


    pub_type: 杂志文章


    authors: Schwarzberg H,Kovács GL,Telegdy G

    更新日期:1984-03-01 00:00:00

  • Altered serum stress neuropeptide levels in critically ill individuals and associations with lymphocyte populations.

    abstract:OBJECTIVE:Potential physiological correlates of stress and the role of stress neuropeptides, other than those of the hypothalamic-pituitary-adrenal axis, in critical illness have not been addressed. We investigated: (a) serum levels of stress neuropeptides (ACTH, substance P (SP), neuropeptide Y (NPY), cortisol, prolac...


    pub_type: 杂志文章


    authors: Mpouzika MD,Papathanassoglou ED,Giannakopoulou M,Bozas E,Middleton N,Boti S,Patiraki EI,Karabinis A

    更新日期:2013-02-01 00:00:00