Abstract：
:Cholecystokinin (CCK) is a widely expressed neuropeptide system originally discovered in the gut. Both cerebral and peripheral neurons as well as endocrine I-cells in the small intestine process proCCK to tyrosyl-O-sulfated and α-carboxyamidated peptides. Recently, we reported that gut endocrine I-cells also synthetize nonsulfated CCK in significant amounts. Accordingly, we have now examined whether porcine and rat cerebral tissues (four cortical regions, hypothalamus and cerebellum) also synthesize nonsulfated CCK. A new, specific radioimmunoassay showed that all brain samples from pigs (n=15) and rats (n=6) contained nonsulfated CCK. The highest concentrations were measured in the neocortex; 4.7±0.25pmol/g (7.4%) in the rat and 4.3±1.88pmol/g (2.3%) in the pig. Chromatography of porcine cortical extracts revealed that 96.4% of the CCK was O-sulfated CCK-8. A higher fraction of the larger peptides (CCK-58 and CCK-33) was nonsulfated in comparison with the shorter forms (CCK-22 and CCK-8), i.e., 8.1% and 4.3% versus 0.9% and 1.5%. Immunohistochemical analysis of the rat brain showed an overall similar distribution pattern in selected regions when comparing the antibody specific for nonsulfated CCK-8 with an antibody recognizing both sulfated and nonsulfated CCK. However, nonsulfated CCK immunoreactivity was stronger than that of sulfated CCK in cell bodies and weaker in nerve terminals. We conclude that only a small fraction of neuronal CCK is nonsulfated. The intracellular distribution of nonsulfated CCK in neurons suggests that they contribute only modestly to the CCK transmitter activity.
journal_name
Neuropeptidesjournal_title
Neuropeptidesauthors
Agersnap M,Zhang MD,Harkany T,Hökfelt T,Rehfeld JFdoi
10.1016/j.npep.2016.08.003subject
Has Abstractpub_date
2016-12-01 00:00:00pages
37-44eissn
0143-4179issn
1532-2785pii
S0143-4179(16)30023-3journal_volume
60pub_type
杂志文章相关文献
NEUROPEPTIDES文献大全abstract：:The neuropeptide galanin is widely expressed in both the central and peripheral nervous systems. However there is limited understanding of how individual galanin receptor (GalR1, 2, and 3) subtypes mediate the physiological activity of galanin in vivo. To address this issue we utilized NAX-5055, a systemically availab...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2015.01.001
更新日期：2015-04-01 00:00:00
abstract：:The role of somatostatin and a stable analogue, sandostatin (Octreotide), on the responses of spinal cord neurones in vivo was investigated in the rat. Electrical C-fibre stimulation was used as a model of acute nociception and the response to subcutaneous formalin was used as a model of longer term events. Intratheca...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(92)90115-d
更新日期：1992-11-01 00:00:00
abstract：:We have previously shown that one of the major determinants directing the expression of the preprotachykinin-A (TAC1) gene, which encodes the neuropeptide substance P, is the transcription factor Neuronal Restrictive Silencer Factor (NSRF), which is also termed Repressor Element-1 Silencing Factor (REST). In rodent mo...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2009.05.004
更新日期：2009-08-01 00:00:00
abstract：:Our previous studies showed the modulation of cocaine and amphetamine regulated transcript (CART) positive neurons and CART mRNA by adrenalectomy and corticosterone replacement in hypothalamic nuclei of male rat brain. More recently, we have shown by CART immunohistochemistry that restraint and forced swim (FS) stress...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2006.02.001
更新日期：2006-06-01 00:00:00
abstract：:Pyroglutamyl diazomethyl ketone and N-benzyloxycarbonyl prolyl prolinal, specific inhibitors of pyroglutamyl peptidase I and prolyl endopeptidase respectively, were used to study the possible role of these enzymes in the regulation of thyrotropin releasing hormone turnover. In vitro thyrotropin releasing hormone relea...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(87)90010-2
更新日期：1987-05-01 00:00:00
abstract：:In the present study, electrophysiological recordings were made from hippocampal slices obtained from mice overexpressing galanin under the promoter for the platelet-derived growth factor-B (GalOE mice). In these mice, a particularly strong galanin expression is seen in the granule cell layer/mossy fibers. Paired-puls...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2005.02.005
更新日期：2005-06-01 00:00:00
abstract：:We have assessed the influence of acute bilateral nephrectomy, of captopril and saralasin, on the hypotensive activity of neurotensin (NT) and of various hypotensive drugs in pentobarbital-anesthetized rats. The results show that the hypotensive activity of NT and of compound 48/80 (C48/80), in contrast to that of his...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(83)90047-1
更新日期：1983-04-01 00:00:00
abstract：:Possible positive reinforcing and aversive effects of intraperitoneally (ip) administered substance P (SP) and its C-terminal heptapeptide analog [pGlu5, MePhe8, Sar9]-SP5-11 (DIME-C7) were investigated in rats. The 'conditioned corral preference and avoidance procedure' was used for assessing positive and negative re...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(90)90037-y
更新日期：1990-12-01 00:00:00
abstract：:The effects of thyroliberin (TRH), a TRH-like tripeptide colon mitosis inhibitor (CMI), a somatostatin analog SMS 201-995 and metoclopramide (dopamine receptor antagonist enhancing prolactin secretion) on rat thymus cell proliferation were investigated. The incorporation of bromodeoxyuridine (BrDU) into thymic cell nu...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(92)90123-e
更新日期：1992-11-01 00:00:00
abstract：:To study the effect of septicaemia, the temporal changes in tissue adrenomedullin (AM) and preproAM mRNA levels were studied in the heart and blood vessels after lipopolysaccharide (LPS) injection. Radioimmunoassay and solution hybridization-RNase protection assays were used to follow the changes in AM and its mRNA le...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2004.11.002
更新日期：2005-04-01 00:00:00
abstract：:Lumbar disc herniation is a common cause of radicular pain, but the mechanism remains ambiguous and the treatment stays unsatisfied. Many studies revealed a traditional Chinese medicine puerarin may moderate chronic pain from diabetes and nerve injury. Thus far, the role and mechanism of puerarin in radicular pain is ...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2018.10.001
更新日期：2018-12-01 00:00:00
abstract：:The hypocretins/orexins (hcrts) are two recently described neuropeptides derived from the same precursor and expressed in a few thousand neurons in the perifornical area of the lateral hypothalamus, which project throughout the brain. The hypocretins bind to two G-protein coupled receptors with different selective aff...
journal_title：Neuropeptides
pub_type： 杂志文章,评审
doi：10.1054/npep.2002.0892
更新日期：2002-04-01 00:00:00
abstract：:Available data concerning the effect of oxytocin on memory are often inconsistent. In the present study it was found that oxytocin, intracerebroventricularly injected to adult male rats in a dose range of 1 fg-10 ng/rat immediately after a 5-minute encounter with a juvenile, significantly reduces the social investigat...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(95)90029-2
更新日期：1995-04-01 00:00:00
abstract：:Social recognition is the sensitive domains of complex behavior critical for identification, interpretation and storage of socially meaningful information. Social recognition develops throughout childhood and adolescent, and is affected in a wide variety of psychiatric disorders. Recently, new data appeared on the mol...
journal_title：Neuropeptides
pub_type： 杂志文章,评审
doi：10.1016/j.npep.2018.09.003
更新日期：2018-12-01 00:00:00
abstract：:Ghrelin (gh) is a peptide hormone that may affect learning and memory. There is some evidence that ghrelin can have antiepileptic effects. So we decided to investigate the possible effects of ghrelin on spatial memory following PTZ-induced seizures in male rats. Ninety male rats were divided into 9 groups including co...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2013.05.005
更新日期：2013-10-01 00:00:00
abstract：:Pituitary adenylate cyclase activating peptide and vasoactive intestinal peptide belong to the same neuropeptide family. Both peptides are present in nerve fibers in the gastric wall and are thought to be involved in the regulation of inflammatory processes. Experimental ulcers were induced in the rat gastric mucosa b...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/s0143-4179(98)90081-6
更新日期：1998-12-01 00:00:00
abstract：:In vivo microdialysis was employed to explore the effects of different selective non-peptides NK(1),NK(2) and NK(3) receptor antagonists on the corticotropin releasing factor (CRF)-induced release of acetylcholine (ACh) in the hippocampus of rats and guinea-pigs. In both species, the intracerebroventricular (i.c.v.) a...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/s0143-4179(03)00019-2
更新日期：2003-04-01 00:00:00
abstract：:The effects of several doses of porcine brain natriuretic peptide-32 (pBNP-32) administered into the lateral brain ventricle were tested as regards the consolidation of passive avoidance learning in rats. The peptide was found to increase the passive avoidance latency in a dose-dependent manner. In order to clarify wh...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(92)90064-4
更新日期：1992-06-01 00:00:00
abstract：:The biological and immunological properties of insulin-like material produced by brain cells from 12 day old mice, cultured in serum-free media were examined. Differences were seen in the rate of appearance, relative amounts and behaviour on HPLC between immunoreactive and biologically active insulin-like material. Ex...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(87)90006-0
更新日期：1987-05-01 00:00:00
abstract：:Alzheimer's disease (AD) is a progressive neurodegenerative disease that deeply affects patients, their family and society. Although scientists have made intense efforts in seeking the cure for AD, no drug available today is able to stop AD progression. In this context, compounds isolated from animal venom are potenti...
journal_title：Neuropeptides
pub_type： 杂志文章,评审
doi：10.1016/j.npep.2017.11.010
更新日期：2018-02-01 00:00:00
abstract：:The selectivity of a series of opioid peptides towards the mu-, delta- and kappa-opioid receptors mediating differential inhibition of electrically-induced neurotransmitter release from rat brain slices was studied, viz. cortical [3H]noradrenaline release (inhibited via mu-receptors), striatal [3H]dopamine release (in...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(89)90065-6
更新日期：1989-08-01 00:00:00
abstract：:The human placenta contains membrane bound opioid receptors. The binding of the antagonist naloxone and the fairly kappa-selective agonist EKC were studied in the microvillous membrane fraction. In both cases high affinity binding sites were detected with Kd values in the nanomolar range. A series of kappa-selective l...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(88)90050-9
更新日期：1988-10-01 00:00:00
abstract：:Highly purified porcine neurophysin-II, prepared from pig posterior pituitary lobe tissue was injected into fifteen rabbits in the preparation of anti-neurophysin sera. All antisera, when used in association with immunohistochemical procedures, gave an immunoreaction in structures of the rat hypothalamo-neurohypophysi...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(83)90038-0
更新日期：1983-10-01 00:00:00
abstract：:The orally active, α-hemoglobin derived hemopressin (PVNFKFLSH, Hp(1-9)) and its truncated (PVNFKFL, Hp(1-7) and PVNFKF, Hp(1-6)) and extended ((R)VDPVNFKFLSH, VD-Hp(1-9) and RVD-Hp(1-9)) derivatives have been postulated to be the endogenous peptide ligands of the cannabinoid receptor type 1 (CB1). In an attempt to cr...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2016.02.001
更新日期：2016-08-01 00:00:00
abstract：:Hippocampal neurogenesis is important for modulating the behavioural responses to stress and for certain forms of learning and memory. The mechanisms underlying the necessary coupling of neuronal activity to neural stem/progenitor cell (NSPC) function remain poorly understood. Within the dentate subgranular stem cell ...
journal_title：Neuropeptides
pub_type： 杂志文章,评审
doi：10.1016/j.npep.2013.10.002
更新日期：2013-12-01 00:00:00
abstract：:Male Wistar rats were injected intraperitoneally with chlorpromazine (2 or 10 mg/kg), haloperidol (0.5 or 2 mg/kg), sulpiride (50 or 100 mg/kg) or clozapine (10 or 25 mg/kg) once, for 14 or 28 consecutive days. Hypothalamic neuropeptide Y-like (NPY-like) immunoreactivity (NPY-LI) was determined 24 h after the last dos...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/s0143-4179(96)90012-8
更新日期：1996-10-01 00:00:00
abstract：:Alarin is a newly identified member of the galanin family of neuropeptides that includes galanin-like peptide (GALP) and galanin. Alarin was discovered as an alternate transcript of the GALP gene in neuroblastoma cells, and subsequently alarin mRNA was detected in the brain of rodents. GALP and galanin are important c...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2010.04.001
更新日期：2010-08-01 00:00:00
abstract：:Endothelins (ET) are a family of highly active neuropeptides with manifold influences via ET receptors (ETR) in both the peripheral and central nervous systems. We have shown previously that satellite glial cells (SGCs) in mouse trigeminal ganglia (TG) are extremely sensitive to ET-1 in evoking [Ca2+]in increase, appa...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2017.03.002
更新日期：2017-06-01 00:00:00
abstract：:We have evaluated the peripheral blood natural killer (NK) cell activity and the in vitro effect of recombinant gamma-interferon (r gamma-IFN) on NK cell activity in 23 patients with a neuroendocrine tumour of the pancreas, small intestine or liver, and 23 healthy controls. Patients with a gastrinoma showed a NK cell ...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/0143-4179(91)90033-f
更新日期：1991-09-01 00:00:00
abstract：:Bone cancer pain (BCP) is a common source of pain in patients with advanced stage and metastatic cancer; however, existing treatment for this kind of pain remains deficient. Being closely related to sensory change and inflammatory pain in both the central and peripheral nervous systems, endothelin A receptor (ETAR) pl...
journal_title：Neuropeptides
pub_type： 杂志文章
doi：10.1016/j.npep.2018.01.003
更新日期：2018-04-01 00:00:00
