Abstract:
:The zona pellucida surrounding ovulated mouse eggs contains three glycoproteins, two of which (ZP2 and ZP3) are reported sperm receptors. After fertilization, the zona pellucida is modified ad minimus by cleavage of ZP2, and sperm no longer bind. Crosstaxa sperm binding is limited among mammals, and human sperm do not bind to mouse eggs. Using transgenesis to replace mouse ZP2 and/or ZP3 with human homologs, mouse lines with human-mouse chimeric zonae pellucidae have been established. Unexpectedly, mouse, but not human, sperm bind to huZP2 and huZP2/huZP3 rescue eggs, eggs fertilized in vitro with mouse sperm progress to two-cell embryos, and rescue mice are fertile. Also unanticipated, human ZP2 remains uncleaved after fertilization, and mouse sperm continue to bind early rescue embryos. These observations are consistent with a model in which the supramolecular structure of the zona pellucida necessary for sperm binding is modulated by the cleavage status of ZP2.
journal_name
Dev Celljournal_title
Developmental cellauthors
Rankin TL,Coleman JS,Epifano O,Hoodbhoy T,Turner SG,Castle PE,Lee E,Gore-Langton R,Dean Jdoi
10.1016/s1534-5807(03)00195-3keywords:
subject
Has Abstractpub_date
2003-07-01 00:00:00pages
33-43issue
1eissn
1534-5807issn
1878-1551pii
S1534-5807(03)00195-3journal_volume
5pub_type
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