Abstract:
:Many promoters in eukaryotes have nucleosome-depleted regions (NDRs) containing transcription factor binding sites. However, the functional significance of NDRs is not well understood. Here, we examine NDR function in two cell cycle-regulated promoters, CLN2pr and HOpr, by varying nucleosomal coverage of the binding sites of their activator, Swi4/Swi6 cell-cycle box (SCB)-binding factor (SBF), and probing the corresponding transcriptional activity in individual cells with time-lapse microscopy. Nucleosome-embedded SCBs do not significantly alter peak expression levels. Instead, they induce bimodal, "on/off" activation in individual cell cycles, which displays short-term memory, or epigenetic inheritance, from the mother cycle. In striking contrast, the same SCBs localized in NDR lead to highly reliable activation, once in every cell cycle. We further demonstrate that the high variability in Cln2p expression induced by the nucleosomal SCBs reduces cell fitness. Therefore, we propose that the NDR function in limiting stochasticity in gene expression promotes the ubiquity and conservation of promoter NDR. PAPERCLIP:
journal_name
Dev Celljournal_title
Developmental cellauthors
Bai L,Charvin G,Siggia ED,Cross FRdoi
10.1016/j.devcel.2010.02.007subject
Has Abstractpub_date
2010-04-20 00:00:00pages
544-55issue
4eissn
1534-5807issn
1878-1551pii
S1534-5807(10)00105-Xjournal_volume
18pub_type
杂志文章abstract::Adult neurogenic niches are present in both vertebrates and invertebrates. Where do stem cells populating these niches originate, and what are the mechanisms maintaining their self-renewal? In this issue of Developmental Cell, Benton et al. (2014) show that in crayfish, hemolymph-derived cells enter a neurogenic niche...
journal_title:Developmental cell
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abstract::Endoplasmic reticulum-plasma membrane (ER-PM) junctions are conserved structures defined as regions of the ER that tightly associate with the plasma membrane. However, little is known about the mechanisms that tether these organelles together and why such connections are maintained. Using a quantitative proteomic appr...
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journal_title:Developmental cell
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journal_title:Developmental cell
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journal_title:Developmental cell
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journal_title:Developmental cell
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journal_title:Developmental cell
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journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2009.10.024
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journal_title:Developmental cell
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journal_title:Developmental cell
pub_type: 评论,杂志文章
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journal_title:Developmental cell
pub_type: 评论,杂志文章
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journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2012.04.010
更新日期:2012-05-15 00:00:00
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journal_title:Developmental cell
pub_type: 杂志文章
doi:10.1016/j.devcel.2006.10.007
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journal_title:Developmental cell
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journal_title:Developmental cell
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