Abstract:
:Dendritic cells (DCs) are antigen presenting cells that play a role in T-cell activation. Liver-associated natural killer T lymphocytes (NKTs) are a unique subset of lymphocytes that may be important in antitumor immunity. Hepatitis B virus (HBV)-associated hepatocellular carcinoma (HCC) expresses hepatitis B virus surface antigen (HBsAg) on its cell surface and may serve as a tumor-associated antigen. The aim of the study was to evaluate the antitumor effect of DC pulsed with tumor or viral-associated antigens in HBV-expressing HCC in mice and to determine the role of NKT lymphocytes in this process. Balb/c mice were sublethally irradiated and transplanted with Hep3b HCC cell line, followed by transplantation of naive splenocytes. DCs were separated using CD11c beads and pulsed with HBV-enveloped proteins (group A), HCC cell lysate (group B), or BSA (control group C). Mice were followed for survival and tumor size. To determine the mechanism of the antitumor effect, intrasplenic and intrahepatic lymphocyte subpopulations were analyzed by FACS for NKT, CD4 and CD8 markers. Tumor-associated antigens-specific IFNgamma ELISPOT, T-cell proliferation assays and serum cytokine analysis were performed. Treatment with tumor-associated antigen-pulsed DC significantly improved survival (40% and 50% as compared with 0% in groups A, B, and control group C, respectively; p < 0.005). Tumor size decreased to 12.8 +/- 0.4 and 0 from 60.4 +/- 0.9 mm(3) in groups A, B, and control group C, respectively (p < 0.005). Adoptive transfer of HBV or Hep3b-associated antigens-pulsed DC induced a 6-fold increase in peripheral CD8(+) lymphocytes (from 1% in control mice to 6% and 5.5% in groups A and B, respectively), along with a decrease in CD4(+) lymphocytes (from 3.5% in controls to 1.4% and 2.3% in A and B, respectively; p < 0.005). The CD8(+)/CD4(+) ratio increased from 0.28 in controls to 4.28 and 2.39 in groups A and B, respectively (p < 0.005). Intrasplenic NKT cells increased from 7% in control mice to 7.98% and 14.6% in groups A and B, respectively. In contrast, an opposite shift was observed inside the liver. Intrahepatic lymphocyte analysis showed a marked increase in CD4(+) and a decrease in CD8(+) lymphocytes in treated groups. The intrahepatic CD4(+) number increased from 0.5% in controls to 2.15% and 25.8% in groups A and B, respectively (p < 0.005). In contrast, a significant decrease in the intrahepatic CD8(+) numbers was observed (from 7% in controls to 1.0% and 2.4% in groups A and B, respectively; p < 0.005). A significant increase was noted in HBV-specific IFNgamma spot-forming T-cell colonies from 0.0 to 8.8 +/- 1.7 and 1.8 +/- 2.9 in groups C, A, and B, respectively (p < 0.005). Similarly, a significant increase in the HBV-specific T-cell stimulation index, from 0.8 +/- 0.2 to 7.2 +/- 0.4, in groups C and B, respectively, was noted (p < 0.002). IFNgamma and IL12 serum levels increased significantly in treated groups. IFNgamma and IL12 serum levels increased to 380 +/- 30 and 400 +/- 20, and 960 +/- 40 and 760 +/- 60 in groups A and B, compared with 150 +/- 16 and 490 +/- 40 pg/ml in control mice (p < 0.005). Tumor antigen-pulsed DCs effectively suppressed the growth of hepatocellular carcinoma in mice. This effect was associated with enhanced NKT and CD8(+) lymphocyte function and augmentation of the antitumor/antiviral-specific IFNgamma production.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Shibolet O,Alper R,Zlotogarov L,Thalenfeld B,Engelhardt D,Rabbani E,Ilan Ydoi
10.1002/ijc.11201keywords:
subject
Has Abstractpub_date
2003-08-20 00:00:00pages
236-43issue
2eissn
0020-7136issn
1097-0215journal_volume
106pub_type
杂志文章abstract::A novel cell culture system is reported for the growth of ovarian tumors. Two approaches were developed to isolate tumor cells, one for ovarian carcinomas and the other for benign cystomas or borderline cystadenomas, which yield virtually pure tumor-cell clusters. The plating efficiency exceeded 10% in approximately 8...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910410606
更新日期:1988-06-15 00:00:00
abstract::Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal tumors of the digestive tract. These tumors span a wide clinical spectrum from benign to malignant and have long been recognized for their nearly absolute resistance to chemotherapy and radiation treatment. We reviewed the worldwide experience on ...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.11374
更新日期:2003-11-01 00:00:00
abstract::Many recent data indicate that some alleles encoded in the central major histocompatibility complex (MHC) region (Class III) of short arm of chromosome 6 may modify the risk of cancer development. Therefore we determined 4 single nucleotide polymorphisms (SNPs) of this region (TNF-alpha -308 G > A, RAGE -429 T > C, HS...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22922
更新日期:2007-10-15 00:00:00
abstract::Human T-lymphotropic virus type I (HTLV-I) carriers often have abnormal lymphocytes (Ably) that resemble malignant cells of adult T-cell leukemia (ATL). To identify predictors of the level of Ably in a longitudinal study of asymptomatic HTLV-I carriers, we analyzed data from 215 subjects (67 men and 148 women) with mu...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980717)77:2<188::aid-ijc
更新日期:1998-07-17 00:00:00
abstract::The utility of EBV load as a tumor marker in nasopharyngeal carcinoma (NPC) patients suggests that it might also serve as a screening test for individuals who are at high risk for developing NPC. We previously demonstrated that unaffected individuals from high-risk families had elevated anti-EBV antibody levels compar...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1002/ijc.21396
更新日期:2006-02-01 00:00:00
abstract::Caspase recruitment domain-containing protein 9 (CARD9) is an adaptor protein and highly expressed in myeloid cells. Our previous study demonstrates a critical protective effect of CARD9 in the development of colitis-associated colon cancer. Nevertheless, the effect of CARD9 in lung cancer remains unclear. Here, using...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32355
更新日期:2019-10-15 00:00:00
abstract::Cancer survival varies by place of residence, but it remains uncertain whether this reflects differences in tumour, patient and treatment characteristics (including tumour stage, indicators of socioeconomic status (SES), comorbidity and information on received surgery and radiotherapy) or possibly regional differences...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29967
更新日期:2016-05-01 00:00:00
abstract::The peripheral blood monocyte is the reactive cell in the tube LAI assay. The monocyte loses its properties of adherence to glass upon exposure to specific antigen. Two different experiments to determine if lymphocytes, when they reacted with tumour, released mediators that were responsible for inhibiting monocyte gla...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910180108
更新日期:1976-07-15 00:00:00
abstract::Telomerase activity is involved in the maintenance of telomere length and is thought to be required for cellular immortality and oncogenesis. Three major subunits composing telomerase, human telomerase RNA (hTR), telomerase-associated protein (TPI) and human telomerase catalytic subunit (hTERT), have been identified. ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990315)80:6<804::aid-ijc
更新日期:1999-03-15 00:00:00
abstract::Results of epidemiologic studies of physical activity and ovarian cancer risk are inconsistent. Few have attempted to measure physical activity over the lifetime or in specific age windows, which may better capture etiologically relevant exposures. We examined participation in moderate-to-vigorous recreational physica...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32513
更新日期:2020-04-01 00:00:00
abstract::We have induced plasmacytomas (MPC) in BALB/c radiochimeras (RCh) repopulated with syngeneic hemopoietic cells carrying distinctive chromosomal markers. A group of 38 RCh that received 0.5 ml pristane, followed by Abelson virus infection 2-3 weeks later, developed 7 tumors (18.5%) of donor origin after a relatively sh...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910440225
更新日期:1989-08-15 00:00:00
abstract::Corpus uteri cancer is the most common gynecological malignancy in most developed countries. The disease is typically diagnosed at an early stage, is of endometrioid histologic subtype, and has a fairly good prognosis. Here, we describe hysterectomy-corrected mortality rates of corpus uteri cancer, overall and stratif...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.33219
更新日期:2021-02-01 00:00:00
abstract::The c-KIT protooncogene encodes a tyrosine kinase receptor, KIT, that is expressed in many normal and cancerous tissues. In this study, we have examined the expression of c-KIT and its ligand, stem cell factor (SCF), in human epithelial ovarian tumors, in normal ovaries and in cultured ovarian surface epithelium (OSE)...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(20000520)89:3<242::aid-ijc6>3.0.
更新日期:2000-05-20 00:00:00
abstract::People living with lymphohematopoietic neoplasms (LHNs) are known to have increased risks of second cancer; however, the incidence of second cancers after LHNs has not been studied extensively in Australia. The Australian Cancer Database was used to analyze site-specific risk of second primary cancer after LHNs in 127...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25706
更新日期:2011-08-15 00:00:00
abstract::Natural cell-mediated immunity against autologous tumor cells, autologous endometrial epithelium, and allogeneic epidermoid carcinoma cell line HeLa was tested in 8 patients with endometrial carcinoma and one patient with endometrial stromal sarcoma. The average cytotoxicity of unstimulated peripheral blood lymphocyte...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910400408
更新日期:1987-10-15 00:00:00
abstract::We previously determined that human papillomavirus (HPV) virus-like particles (VLPs) and pseudovirions (PsV) did not, respectively, bind to or infect intact epithelium of the cervicovaginal tract. However, they strongly bound heparan sulfate proteoglycans (HSPG) on the basement membrane of disrupted epithelium and inf...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29823
更新日期:2016-02-15 00:00:00
abstract::Although viral sequences closely related to feline leukaemia virus are represented in multiple copies in cellular DNA of all domestic cats, a specific fraction was present only in the virus-infected cells. This fraction was detected by viral cDNA enriched by a prior absorption of a total complementary DNA (cDNA) trans...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910220114
更新日期:1978-07-15 00:00:00
abstract::The site-specific relationship between melanoma and sun exposure was investigated in the high-risk region of New Zealand. Age and latitude of residence were used as biological and geographical proxy measures of exposure for the 16,117 newly incident cases and 3,150 death records reported between 1968 and 1993. Age-sta...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(20000301)85:5<627::aid-ijc
更新日期:2000-03-01 00:00:00
abstract::The aim of this study was to test the utility of AIMP3, an upstream regulator of DNA damage response following genotoxic stress, as a clinical biomarker in muscle-invasive bladder cancer (MIBC). AIMP3 was identified from a meta-analysis of a global gene-expression dataset. AIMP3 protein expression was determined by im...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.29022
更新日期:2015-02-01 00:00:00
abstract::Bcl-2 protein inhibits apoptosis, reduces the requirement for growth factors, and thereby extends the survival of cells. Recent findings of Bcl-2 in several solid tumors suggest that it might contribute to the genesis of some types of cancer. Over-expression of Bcl-2 might play a role in carcinogenesis and malignant p...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980417)79:2<153::aid-ijc
更新日期:1998-04-17 00:00:00
abstract::The variable domain of functional heavy chain antibodies (VHH) devoid of light chains, found in camels, constitute the smallest intact antigen-binding domain fragment. Two camel single-domain fragments, cAb-Lys2 and cAb-Lys3, recognizing an overlapping epitope of lysozyme with a dissociation constant of 2 nM and 65 nM...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10212
更新日期:2002-03-20 00:00:00
abstract::In biliary tract cancer (BTC), tissue biopsies to guide treatment are rarely feasible, thus implementing liquid biopsy approaches to improve patient management represents a priority. So far, studies on circulating tumor cells (CTCs) in BTC are insufficient to promote their use in patient clinical management and are li...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32822
更新日期:2020-06-15 00:00:00
abstract::Cigarette smoking and alcohol consumption were examined as potential risk factors in a case-control study of primary liver cancer (PLC). A total of 165 PLC cases and 465 matched controls from several US hospitals were studied. A weak but statistically significant (p less than 0.05) dose-response relationship was obser...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910420304
更新日期:1988-09-15 00:00:00
abstract::Improved biomarkers for prostate cancer (PC) risk stratification are urgently needed. Here, we aimed to develop a novel multimarker model for prediction of biochemical recurrence (BCR) after curatively intended radical prostatectomy (RP), based on minimally invasive sampling of blood and urine. We initially measured t...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32296
更新日期:2019-11-01 00:00:00
abstract::Nanoparticle-based phototherapies, such as photothermal therapy (PTT) and photodynamic therapy (PDT), exhibit strong efficacy, minimal invasion and negligible side effects in tumor treatment. These phototherapies have received considerable attention and been extensively studied in recent years. In addition to directly...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.31717
更新日期:2018-12-15 00:00:00
abstract::Randomized clinical trials have shown that use of 5α-reductase inhibitors (5-ARIs) lowers overall prostate cancer (PCa) risk compared to placebo, while the proportion of Gleason 8-10 tumors is elevated. It is unknown whether this affects PCa-specific survival. We studied disease-specific survival by 5-ARI usage in a c...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30017
更新日期:2016-06-15 00:00:00
abstract::Resistance to anticancer drugs is the major problem in the treatment of many advanced cancers, including androgen-independent prostate cancer. Recently, increased expression of Id-1, a basic helix-loop-helix protein, is reported in several types of advanced cancer. It is suggested that high expression of Id-1 may prov...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21592
更新日期:2006-04-15 00:00:00
abstract::Some herds of miniature swine are genetically predisposed to cutaneous melanoma. To test if swine melanoma susceptibility could be linked to the CDKN2A gene, which is involved in a proportion of 9p21-linked human familial melanoma, we performed a genetic analysis of miniature pigs of the MeLiM strain. F(1) and backcro...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10871
更新日期:2003-02-20 00:00:00
abstract::Production of interleukin-6 (IL-6) by human pancreatic carcinoma cells inversely correlates with potentials for blood-borne metastasis to the liver in nude mice. IL-6 cDNA was transfected to PCI-43, one of our cultured pancreatic carcinoma cell lines that does not produce IL-6 and generates numerous metastases to the ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980119)75:2<284::aid-ijc
更新日期:1998-01-19 00:00:00
abstract::Liquid-based cytology (LBC) has replaced conventional cytology (CC) for cervical cancer screening in some countries. However, it remains unclear whether LBC is superior to CC. A randomized controlled trial was conducted between August 2007 and March 2009 in Germany to compare LBC, alone and in combination with compute...
journal_title:International journal of cancer
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1002/ijc.27955
更新日期:2013-06-15 00:00:00