Abstract:
:A novel cell culture system is reported for the growth of ovarian tumors. Two approaches were developed to isolate tumor cells, one for ovarian carcinomas and the other for benign cystomas or borderline cystadenomas, which yield virtually pure tumor-cell clusters. The plating efficiency exceeded 10% in approximately 80% of the processed surgical specimens. Cells grown in a newly developed KOV medium (a modification of MCDB 151 supplemented with 6 defined growth factors and a moderate amount of FBS) had an average growth rate of 0.23 population doublings/day. Primary tumor-derived cultures, including those derived from cystomas, were analyzed by flow cytometry demonstrating a DNA heteroploid content in 55% of the cases. The neoplastic origin of the cells in culture was further confirmed by 3 monoclonal antibodies (OC125; MOv2; MOv19) with high specificity against epithelial ovarian malignancies. Cultures were tested with cis-DDP to determine their suitability for pharmacological studies. Exposure to the drug (from 10 to 80 microM for 1 hr) resulted in variable cell-killing responses, and the prominent effect on cell-cycle progression in primary cultures was a prolonged arrest in S phase. The formation and persistence of DNA-ISC caused by an exposure to 40 microM cis-DDP for 1 hr was studied by alkaline elution in 6 different tumor-derived cultures. DNA-ISC equivalents were highest between 9 and 24 hr after treatment and were repaired only to a limited extent within 48 hr of recovery time. The present study confirms the usefulness of this culture system for pharmacological studies of active chemotherapeutic agents against human ovarian tumors.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Balconi G,Broggini M,Erba E,D'Incalci M,Colombo N,Mangioni C,Bertolero Fdoi
10.1002/ijc.2910410606subject
Has Abstractpub_date
1988-06-15 00:00:00pages
809-18issue
6eissn
0020-7136issn
1097-0215journal_volume
41pub_type
杂志文章abstract::Ninety-seven Japanese patients with so-called primary non-Hodgkin's lymphoma of the central nervous system (CNS-NHL), unrelated to the acquired immunodeficiency syndrome (AIDS) or organ transplantation, were reviewed. The patients' ages ranged from 1 to 87 years (median: 58 years) with a male to female ratio of 1.77:1...
journal_title:International journal of cancer
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doi:10.1002/ijc.2910450410
更新日期:1990-04-15 00:00:00
abstract::Human papilloma virus (HPV) infection is considered as an important aetiological factor for anal squamous cell carcinoma (ASCC) but is not sufficient for tumour progression. This carcinoma is poorly understood at the molecular level. Using the largest cohort of cases to date we investigated the molecular mechanisms un...
journal_title:International journal of cancer
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journal_title:International journal of cancer
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abstract::Tumorigenic clonal lines derived from soft agar colonies induced by DMBA-transformed feline embryo cells were isolated and characterized. The morphologically altered clonal cells formed large aggregates, growing in this aggregate form when suspended in liquid growth medium above an agar base and forming colonies in so...
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journal_title:International journal of cancer
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journal_title:International journal of cancer
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doi:
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abstract::In order to study the mechanisms responsible for resistance to CDDP, 5 human tumor cell lines were made resistant to CDDP by repeated in vitro exposures. After cloning it was found that the cell lines developed were between 3.3-fold and 17-fold more resistant to CDDP than the parental cell lines at the IC90. These lin...
journal_title:International journal of cancer
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journal_title:International journal of cancer
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doi:10.1002/ijc.2910570421
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journal_title:International journal of cancer
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更新日期:1986-08-15 00:00:00
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journal_title:International journal of cancer
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doi:10.1002/ijc.20931
更新日期:2005-07-10 00:00:00
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journal_title:International journal of cancer
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