Abstract:
:The surface and intracellular expression of mdrI-P-glycoprotein in parental drug-sensitive human breast cancer cells (MCF-7) and their multidrug-resistant (MDR) variants has been studied by using the monoclonal antibodies (MAbs) MM4.17 and MRK-16, which recognize 2 different epitopes of the drug efflux pump molecule. Fluorescence microscopic observations showed that P-glycoprotein, in addition to being located at the cell surface, can also be found in the Golgi apparatus of resistant cells. To confirm this finding, Golgi apparatus and P-glycoprotein were double-labelled with wheat-germ agglutinin (WGA) and MAb MM4.17. Laser scanning confocal microscopy indicated that, in MDR cells, Adriamycin mainly accumulated cytoplasmically in a perinuclear region. This accumulation proved to be modulated by pre-treatment with verapamil or ATP depletion. Moreover, the vital staining of Adriamycin-treated MDR cells, performed with the fluorescent lipid N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]- 6-aminocaproyl sphingosine (C6-NBD-ceramide), revealed that the anthracyclinic antibiotic was located in the Golgi apparatus. All these results indicate that the drug transporter is located in the Golgi apparatus, in which Adriamycin molecules also accumulate.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Molinari A,Cianfriglia M,Meschini S,Calcabrini A,Arancia Gdoi
10.1002/ijc.2910590614subject
Has Abstractpub_date
1994-12-15 00:00:00pages
789-95issue
6eissn
0020-7136issn
1097-0215journal_volume
59pub_type
杂志文章abstract::In vitro infection of EBV-negative lymphoma cell lines with immortalizing strains of Epstein-Barr virus induces the cell-surface expression of B-cell markers, such as the EBV receptor/CR2 (CD21) and the CD23 antigen. The non-immortalizing EBV variant, P3HRI, which carries a deletion encompassing the EBV nuclear antige...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910460418
更新日期:1990-10-15 00:00:00
abstract::It has been proposed that oxidative stress develops in tumors, with important consequences for growth and progression. To investigate this hypothesis, we measured low m.w. thiols, disulfides, protein-mixed disulfides and a pool of major anti-oxidant enzymes in renal-cortex as well as renal-cell carcinoma (RCC) specime...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/1097-0215(20010101)91:1<55::aid-ijc1006>3.
更新日期:2001-01-01 00:00:00
abstract::Nuclear factor-κB (NF-κB) is constitutively activated in many cancers, including oral squamous cell carcinoma (OSCC), and is involved in the invasive characteristics of OSCC, such as growth, antiapoptotic activity and protease production. However, the cellular mechanism underlying NF-κB's promotion of bone invasion by...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27435
更新日期:2012-09-01 00:00:00
abstract::Experimental and epidemiological data support a role for sex steroid hormones in the pathogenesis of endometrial cancer. The associations of pre-diagnostic blood concentrations of estradiol, estrone, testosterone, androstenedione, DHEAS and SHBG with endometrial cancer risk were investigated. A case-control study was ...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.11529
更新日期:2004-01-20 00:00:00
abstract::Several promising chemopreventive agents have for lung cancer emerged in preclinical models and in retrospective trials. These agents have been shown to modulate pathways altered in carcinogenesis and reduce markers of carcinogenesis in animal and cell culture models. Cancer-prone transgenic mice with oncogenic Kras e...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31152
更新日期:2018-04-01 00:00:00
abstract::To elucidate the individual impacts of insulin and blood glucose on cancer risk, we investigated the association of plasma C-peptide, a surrogated marker of insulin and glycated albumin (GA), a more stable marker of blood glucose, with all-site and site-specific cancer risk by mutually accounting for their confounding...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31847
更新日期:2019-02-15 00:00:00
abstract::The influence of 4 murine monoclonal antibodies (MAbs) directed against surface determinants of a metastasizing rat adenocarcinoma (BSp73ASML) on metastatic spread was evaluated and compared to their in vivo binding as well as to the induction of a humoral anti-MAb response, especially with respect to the development ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910460528
更新日期:1990-11-15 00:00:00
abstract::Gefitinib has shown meaningful antitumor activity with tolerable toxicity in chemotherapy-refractory NSCLC in previous studies. Moreover, EGFR expression failed to show a correlation with response. In an attempt to identify predictive markers of response, we have investigated the tumoral expression of key signaling mo...
journal_title:International journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1002/ijc.20550
更新日期:2005-01-01 00:00:00
abstract::Accurate survival estimates are essential for monitoring cancer survival trends, for health care planning and for resource allocation. To obtain precise estimates of survival, full dates (day, month and year) rather than partial dates (month and year) are required. In some jurisdictions, however, cancer registries are...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.27545
更新日期:2012-10-01 00:00:00
abstract::Due to the large scale use of asbestos (more than 3.5 million tons produced or imported until its definitive banning in 1992), a specific national surveillance system of mesothelioma incident cases is active in Italy, with direct and individual anamnestic etiological investigation. In the period between 1993 and 2004,...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26229
更新日期:2012-05-01 00:00:00
abstract::Human T-lymphotropic virus type I (HTLV-I) carriers often have abnormal lymphocytes (Ably) that resemble malignant cells of adult T-cell leukemia (ATL). To identify predictors of the level of Ably in a longitudinal study of asymptomatic HTLV-I carriers, we analyzed data from 215 subjects (67 men and 148 women) with mu...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980717)77:2<188::aid-ijc
更新日期:1998-07-17 00:00:00
abstract::Nasopharyngeal carcinoma (NPC) is an Epstein-Barr virus (EBV)-associated lymphoepithelioma. The aim of this study was to characterize the homogeneity and distinctness of the T-cell repertoires within and between primary and metastatic NPCs. We used ultra-deep sequencing of the hypervariably rearranged antigen-binding ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31336
更新日期:2018-08-01 00:00:00
abstract::Serum samples from feral populations of African green monkeys (Cercopithecus aethiops) were screened for antibodies to the simian T-lymphotropic virus, type I (STLV-I). Blood samples had been collected from 336 monkeys in 4 regions of central and southern Kenya in 1978 and 1979, from 114 monkeys in central Ethiopia in...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910380412
更新日期:1986-10-15 00:00:00
abstract::Signal transduction pathways controlling tumor cell locomotion are not yet well understood. We have studied the role of protein kinase C (PKC)-dependent protein phosphorylation associated with changes in cell shape and locomotor activity of Walker carcinosarcoma cells in culture. We show that the inhibitory effect of ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960208)65:4<473::AID-IJC
更新日期:1996-02-08 00:00:00
abstract::We examined the biologic properties of a small-cell-lung-carcinoma (SCLC) cell line (designated MN-1112) established from a patient with SCLC who showed paraneoplastic retinopathy syndrome. Morphologic and immunocytochemical analyses showed that MN-1112 cells possess features of the classic type of SCLC. MN-1112 cells...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960301)65:5<671::AID-IJC
更新日期:1996-03-01 00:00:00
abstract::We showed earlier that interferon-gamma is a powerful inducer of differentiation of human neuroblastoma (NB) cells. Although 2',5' oligo-adenylate synthetase (2,5 OAS) may play a role in mediating the anti-proliferative and/or differentiative effects of interferons (IFNs), direct evidence is lacking. We have investiga...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910620219
更新日期:1995-07-17 00:00:00
abstract::By radioimmunoassay (RIA) mammary tumour virus (MTV) antigens were detected in individual milk samples of C3Hf mice, (female BALB/c X male C3Hf)F1 mice and (female C3Hf X male BALB/c)F1 mice; milk samples of BALB/c mice were negative. In the segregating backcross I population, female BALB/c X male (female BALB/c X mal...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910160606
更新日期:1975-12-15 00:00:00
abstract::Spontaneous tumor development and primary oncogenesis were compared in a large number of NK4-deficient, homozygous C57Bl/6-bg/bg mice and their NK normal, heterozygous +/bg littermate controls. In a group of 167 retired breeders followed for spontaneous tumors, the probability of survival for mice eventually dying wit...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910350414
更新日期:1985-04-15 00:00:00
abstract::Adult T-cell leukemia (ATL) is a retrovirus-associated leukemia with poor prognosis and often has deletions of the p16INK4a and p15INK4b genes on chromosome 9p21. The gene for methylthioadenosine phosphorylase (MTAP), a purine and methionine metabolic enzyme, resides approximately 100 Kb telomeric to the p16INK4a gene...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980105)75:1<51::aid-ijc9
更新日期:1998-01-05 00:00:00
abstract::Cancer incidence rates are presented for the Nairobi Cancer Registry, a population-based cancer registry (PBCR) covering the population of the capital city of Kenya (3.2 million inhabitants in 2009). Case finding was by active methods, with standard and checks for accuracy and validity. During the period 2004-2008 a t...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.29674
更新日期:2015-11-01 00:00:00
abstract::Dietary patterns reflect combinations of dietary exposures, and here we examine these in relation to prostate cancer risk. In a case-control study, 80 incident primary prostate cancer cases and 334 urology clinic controls were enrolled from 1997 through 1999 in Kingston, Ontario, Canada. Food-frequency questionnaires ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21112
更新日期:2005-09-10 00:00:00
abstract::Malignant mesothelioma (MM) is an aggressive tumor associated with environmental or occupational exposure to asbestos fibers. Erionite is a fibrous zeolite, morphologically similar to asbestos and it is assumed to be even more carcinogenic. Onset and progression of MM has been suggested as the result of the cooperatio...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22687
更新日期:2007-07-01 00:00:00
abstract::As part of a collaborative study of risk factors for childhood brain tumours, the effects of the mother's smoking and her potential for passive smoking exposure during the pregnancy were assessed in a case-control study. Parents of 91 cases and 321 population controls from Northern Italy, matched for age, sex and resi...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.2910570602
更新日期:1994-06-15 00:00:00
abstract::The early arrest patterns of a methylcholanthrene-induced fibrosarcoma were determined after injection of 125IUdR-labelled cells into the systemic circulation of mice bearing transplanted lymphosarcomas, and of normal animals. Similar experiments were performed using radiolabelled lymphosarcoma cells and normal or fib...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910180608
更新日期:1976-12-15 00:00:00
abstract::Cytotoxic antibodies against glioblastoma-associated antigen(s) have been sought for in glioma patient sera. Complement-dependent cytotoxicity (CDC) I and antibody-dependent cell-mediated cytotoxicity (ADCC) assays were used to test sera from 80 patients using 51 Cr labelled target cells derived from eight different g...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910250209
更新日期:1980-02-15 00:00:00
abstract::The radiosensitivity of three forms of response to injection of C. parvum in mice has been investigated. The increase in phagocytin index evoked by IP or IV injection of 0.7 mg C. parvum (but not that evoked by 1.4 mg) was reduced but not abolished in mice given 350-500 rad whole-body irradiation 4 days before C. parv...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910170515
更新日期:1976-05-15 00:00:00
abstract::Metabolic syndrome (MetS) and its components may link to pancreatic cancer risk; however, current epidemiological evidence is limited, and the potential mechanisms underlying the associations remain unclear. To investigate this, we carried out this prospective cohort study of 474 929 participants without a diagnosis o...
journal_title:International journal of cancer
pub_type: 杂志文章,评审
doi:10.1002/ijc.33172
更新日期:2020-12-15 00:00:00
abstract::A recent clinical trial found a protective role of niacinamide, a derivative of niacin, against skin cancer recurrence. However, there is no epidemiologic study to assess the association between niacin intake and risk of skin cancer [basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and melanoma]. We prospecti...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30630
更新日期:2017-05-01 00:00:00
abstract::A triazene-xenogenized tumor sub-line was derived from the mouse mastocytoma cell line P815 following several transplant generations in vivo on DTIC. The highly immunogenic P815/DTIC variant line expressed new CTL-defined antigens. Novel antigens were also detected by antibodies in immunoprecipitation and by Western b...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910550227
更新日期:1993-09-09 00:00:00
abstract::Tissue factor (TF)-mediated protease-activated receptor (PAR)-2 signaling is associated with a promigratory, invasive and proangiogenic phenotype in experimental models of breast cancer and has been mechanistically coupled to phosphorylation of the TF cytoplasmic domain (pTF). However, the clinical relevance of these ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.24921
更新日期:2010-05-15 00:00:00