Inhibition of protein kinase C-dependent protein phosphorylation correlates with increased polarity and locomotion in Walker 256 carcinosarcoma cells.


:Signal transduction pathways controlling tumor cell locomotion are not yet well understood. We have studied the role of protein kinase C (PKC)-dependent protein phosphorylation associated with changes in cell shape and locomotor activity of Walker carcinosarcoma cells in culture. We show that the inhibitory effect of phorbol-12-myristate-13-acetate (PMA), an activator of PKC, on cell polarity and locomotion can be suppressed by the PKC-selective inhibitor Ro 31-8220. PMA induces increased phosphorylation of at least 2 proteins, of 65 and 80 kDa, in intact Walker carcinosarcoma cells. These bands are enriched in cytosolic fractions isolated from cells incubated with 32PO4. Pre-incubation with Ro 31-8220 inhibits the PMA-induced phosphorylation of both bands in a concentration-dependent manner. This effect is very likely not due to inhibition of translocation of PKC to the membrane as Ro 31-8220 enhances, rather than inhibits, PMA-induced transfer of PKC beta(II) to the particulate fraction. We have carried out a quantitative analysis of phosphorylation of the 80-kDa band. Ro 31-8220 reverses both PMA-induced phosphorylation of this band and PMA-induced suppression of cell polarity in parallel. Increased phosphorylation of proteins via PKC may thus be a stop signal for locomoting Walker carcinosarcoma cells.


Int J Cancer


Niggli V,Zimmermann A,Keller H




Has Abstract


1996-02-08 00:00:00














  • Application of differential display to identify genes for lung cancer detection in peripheral blood.

    abstract::A blood assay for detection of lung cancer biomarkers could significantly improve cancer patient prognosis and survival rates. Amplified fragment length polymorphism-differential display (AFLP-DD) was used to identify gene transcripts found in lung cancer tissue and the peripheral blood of lung cancer patients. The cl...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Matsunaga H,Hangai N,Aso Y,Okano K,Kawamura M,Kobayashi K,Kambara H,Hoger JH,Mitsuhashi M

    更新日期:2002-08-10 00:00:00

  • Subtypes of Epstein-Barr virus in HIV-1-associated and HIV-1-unrelated Hodgkin's disease cases.

    abstract::Epstein-Barr virus (EBV) type 2 is considered to be a much less potent transformer of lymphocytes than type 1. However, type-2 EBV may be involved in the pathogenesis of non-Hodgkin's lymphomas (NHLs) arising in immunocompromised patients, i.e., subjects with malaria or HIV-1 infection. To determine whether type-2 EBV...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: De Re V,Boiocchi M,De Vita S,Dolcetti R,Gloghini A,Uccini S,Baroni C,Scarpa A,Cattoretti G,Carbone A

    更新日期:1993-07-30 00:00:00

  • Transcription of latent and replicative Epstein-Barr-virus genes in bone-marrow and peripheral-blood mononuclear cells of healthy donors.

    abstract::Reverse-transcriptase polymerase chain reaction has been used to analyze the expression of 2 latent genes (EBNA-1 and LMP-1) and one replicative gene (BZLF-1) of Epstein-Barr virus in mononuclear cells from bone marrow and peripheral blood of healthy donors. EBV-gene transcription was detected in 8 out of 15 bone-marr...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Gonnella R,Angeloni A,Calogero A,Farina A,Santarelli R,Gentile G,Arcese W,Martino P,Mandelli F,Frati L,Faggioni A,Ragona G

    更新日期:1997-03-04 00:00:00

  • Nasopharyngeal carcinoma-susceptibility locus is localized to a 132 kb segment containing HLA-A using high-resolution microsatellite mapping.

    abstract::Nasopharyngeal carcinoma (NPC) is an epithelial tumor uniquely prevalent in southern Chinese. HLA-A2 is associated with NPC. In a previous study, we showed that the genes associated with susceptibility to NPC are primarily located within the HLA-A locus in Taiwanese NPC patients. However, the pathogenic genes causing ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lu CC,Chen JC,Tsai ST,Jin YT,Tsai JC,Chan SH,Su IJ

    更新日期:2005-07-10 00:00:00

  • No excess risk for colorectal cancer among subjects seropositive for the JC polyomavirus.

    abstract::The human polyomaviruses JC virus (JCV) and BK virus (BKV) are oncogenic in experimental systems and commonly infect humans. JCV DNA has been reported to be present in human colon mucosa and in colorectal cancers. To investigate whether the risk for colorectal cancer is associated with JCV or BKV infection, we perform...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lundstig A,Stattin P,Persson K,Sasnauskas K,Viscidi RP,Gislefoss RE,Dillner J

    更新日期:2007-09-01 00:00:00

  • The expression of hematopoietic progenitor cell antigen CD34 is regulated by DNA methylation in a site-dependent manner in gastrointestinal stromal tumours.

    abstract::The anatomic site-dependent expression of hematopoietic progenitor cell antigen CD34 is a feature of gastrointestinal stromal tumours (GISTs). The basis for the differential CD34 expression is only incompletely understood. This study aimed at understanding the regulation of CD34 in GISTs and clarification of its site-...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Bure I,Braun A,Kayser C,Geddert H,Schaefer IM,Cameron S,Ghadimi MB,Ströbel P,Werner M,Hartmann A,Wiemann S,Agaimy A,Haller F,Moskalev EA

    更新日期:2017-12-01 00:00:00

  • Combined oral contraceptives and liver cancer. The WHO Collaborative Study of Neoplasia and Steroid Contraceptives.

    abstract::A multi-national, hospital-based, case-control study was conducted to evaluate the possible relationships of steroid contraceptives to 6 neoplasms. Based on data from 122 newly diagnosed cases of primary liver cancer and 802 matched controls, the relative risk of liver cancer in women who had ever used combined oral c...

    journal_title:International journal of cancer

    pub_type: 临床试验,杂志文章,多中心研究



    更新日期:1989-02-15 00:00:00

  • Evidence of heterogeneity within colorectal liver metastases for allelic losses, mRNA level expression and in vitro response to chemotherapeutic agents.

    abstract::A goal of oncology is to predict chemosensitivity of tumors. This approach assumes that in a patient all tumor deposits are homogeneous. We have tested the heterogeneity between several samples of the same liver metastasis (LM; intrametastatic heterogeneity) or between multiple LM (intermetastatic heterogeneity) from ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Goasguen N,de Chaisemartin C,Brouquet A,Julié C,Prevost GP,Laurent-Puig P,Penna C

    更新日期:2010-09-01 00:00:00

  • Presence of human papillomavirus type-16 and type-18 DNA sequences and their expression in cervical cancers and cell lines from Japanese patients.

    abstract::Southern blot analyses of surgical specimens of cervical carcinoma from Japanese patients showed that 3/9 samples contained human papillomavirus (HPV) type-16 DNA sequences, and 2 contained HPV type-18 DNA sequences. By Northern blot analyses, RNA transcripts of HPV DNA sequences were demonstrated in some of the tissu...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Tsunokawa Y,Takebe N,Nozawa S,Kasamatsu T,Gissmann L,zur Hausen H,Terada M,Sugimura T

    更新日期:1986-04-15 00:00:00

  • Chromosome-9 loss detected by fluorescence in situ hybridization in bladder cancer.

    abstract::A loss of chromosome-9 material is one of the most frequent genomic aberrations known in bladder cancer. In order to better understand the role of chromosome-9 losses in bladder cancer, 125 formalin-fixed and 37 unfixed bladder tumors were examined using fluorescence in situ hybridization (FISH). A repetitive probe fo...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Sauter G,Moch H,Carroll P,Kerschmann R,Mihatsch MJ,Waldman FM

    更新日期:1995-04-21 00:00:00

  • Gene expression profiling reveals novel biomarkers in nonsmall cell lung cancer.

    abstract::The development of reliable gene expression profiling technology is having an increasing impact on our understanding of lung cancer biology. Our study aimed to determine any correlation between the phenotypic heterogeneity and genetic diversity of lung cancer. Microarray analysis was performed on a set of 46 tumor sam...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Sanchez-Palencia A,Gomez-Morales M,Gomez-Capilla JA,Pedraza V,Boyero L,Rosell R,Fárez-Vidal ME

    更新日期:2011-07-15 00:00:00

  • Concentration and time-dependent inter-relationships for antitumour drug cytotoxicities against tumour cells in vitro.

    abstract::Cryopreserved tumour cells obtained from the ascitic fluid of a patient with an ovarian carcinoma were employed to determine the effect on in vitro drug cytotoxicities of varying both drug concentration and exposure time. Four antitumour drugs, in common clinical usage, were selected for study. Tumour-cell survival fo...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Rupniak HT,Whelan RD,Hill BT

    更新日期:1983-07-15 00:00:00

  • Frequent deletion in chromosome 4 and duplication of chromosome 15 in liver epithelial cells derived from long-term culture of C3H mouse hepatocytes.

    abstract::Prolonged culture of hepatocytes isolated from mouse liver results in the spontaneous development of colonies of liver epithelial cells that can proliferate indefinitely in vitro. We established 5 such cell lines from C3H/HeJ mice (C3H) and 22 cell lines from C3H/HeJ x C57BL/6J F1 mice (C3B6F1) to investigate whether ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Nishimori H,Ogawa K,Tateno H

    更新日期:1994-10-01 00:00:00

  • Correlation of numerical and structural status of chromosome 16 with histological type and grade of non-invasive and invasive breast carcinomas.

    abstract::Loss of heterozygosity on chromosome arm 16q frequently occurs in human breast carcinomas regardless of the histological grade or type. To reveal whether the status of chromosome 16 corresponds to the histology of breast carcinomas, we examined the signal number of 16cen, 16q breakpoints and 1;16 fusions in the interp...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Tsuda H,Takarabe T,Hirohashi S

    更新日期:1999-08-20 00:00:00

  • Increasing risk of uterine cervical cancer among young Japanese women: Comparison of incidence trends in Japan, South Korea and Japanese-Americans between 1985 and 2012.

    abstract::In Japan, cervical cancer incidence has increased since the late 1990s especially among young women, despite a decreasing trend in most developed countries. Here, we examined age, period and birth cohort trends in cervical cancer incidence rates from 1985 to 2012. Incidence rates were ascertained using three populatio...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Utada M,Chernyavskiy P,Lee WJ,Franceschi S,Sauvaget C,de Gonzalez AB,Withrow DR

    更新日期:2019-05-01 00:00:00

  • CpG methylation of viral DNA in EBV-associated tumours.

    abstract::In EBV-immortalized lymphoblastoid cell lines (LCLs) a small number of "latent" proteins are expressed. These are the EBV nuclear antigens, EBNAs 1-6, and a latent membrane protein, LMP. We have investigated the expression of these proteins in a variety of EBV-associated tumours and cell lines. Whereas transplant and ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Allday MJ,Kundu D,Finerty S,Griffin BE

    更新日期:1990-06-15 00:00:00

  • Leukemic progenitor cells are susceptible to targeting by stimulated cytotoxic T cells against immunogenic leukemia-associated antigens.

    abstract::Leukemic stem cells (LSC) might be the source for leukemic disease self-renewal and account for disease relapse after treatment, which makes them a critical target for further therapeutic options. We investigated the role of cytotoxic T-lymphocytes (CTL) counteracting and recognizing LSC. Leukemia-associated antigens ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Schneider V,Zhang L,Rojewski M,Fekete N,Schrezenmeier H,Erle A,Bullinger L,Hofmann S,Götz M,Döhner K,Ihme S,Döhner H,Buske C,Feuring-Buske M,Greiner J

    更新日期:2015-11-01 00:00:00

  • Sucrase-isomaltase expression and enterocytic ultrastructure of human colorectal tumors.

    abstract::We report the relative frequency of sucrase-isomaltase (SI) antigen expression in human colonic adenocarcinoma (22/57), in peritumoral mucosa taken next to the tumor (31/41) or distant from it (29/42) as well as in 21/23 polyps. Our results are based on indirect immunofluorescence with a monoclonal antibody (MAb) spec...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Czernichow B,Simon-Assmann P,Kedinger M,Arnold C,Parache M,Marescaux J,Zweibaum A,Haffen K

    更新日期:1989-08-15 00:00:00

  • Cell-specific targeting of a thymidine kinase/ganciclovir gene therapy system using a recombinant Sindbis virus vector.

    abstract::Transfer of the herpes simplex virus type I thymidine kinase (HSV-TK) gene into tumor cells using virus-based vectors in conjunction with ganciclovir (GCV) exposure provides a potential gene therapy strategy for the treatment of cancer. The possibility of using a novel targetable Sindbis virus expression vector contai...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Iijima Y,Ohno K,Ikeda H,Sawai K,Levin B,Meruelo D

    更新日期:1999-01-05 00:00:00

  • Ganglioside GM2 on the K562 cell line is recognized as a target structure by human natural killer cells.

    abstract::Although human NK cells lyse a wide spectrum of target cells, the precise target structure recognized by NK cells has not yet been elucidated. In order to define a possible relationship between gangliosides on human target cells and susceptibility to NK lysis, 14 human leukemia and lymphoma cell lines were studied. A ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Ando I,Hoon DS,Suzuki Y,Saxton RE,Golub SH,Irie RF

    更新日期:1987-07-15 00:00:00

  • Evidence of enhancement of the ras oncogene protein product (p21) in a spectrum of human tumors.

    abstract::Using a direct binding liquid competition radioimmunoassay, the amount of the ras oncogene protein product, p21, was quantitated in a variety of human tumors and adjacent apparently normal tissues. In 48 of 50 matched tumor and normal tissue biopsy specimens from 50 patients, more ras p21 was detected in the tumor tha...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: De Biasi F,Del Sal G,Hand PH

    更新日期:1989-03-15 00:00:00

  • Establishment of a continuous tumor-cell line (panc-1) from a human carcinoma of the exocrine pancreas.

    abstract::An epithelioid cell line, started from a human pancreatic carcinoma of ductal cell origin, has been maintained in culture for over 2 years and has been subcultured more than 40 times. The PANC-1 cell line has a doubling time of 52 h and G6PD activity of the slow mobility of B type. Chromosome studies show a modal numb...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Lieber M,Mazzetta J,Nelson-Rees W,Kaplan M,Todaro G

    更新日期:1975-05-15 00:00:00

  • Identification of TDE2 gene and its expression in non-small cell lung cancer.

    abstract::TDE2, a gene with sequence similarity to the mouse testicular tumor-differentially-expressed (Tde1/MUSTETU) gene, was identified by serial analysis of gene expression (SAGE) in nonsmall cell lung cancers (NSCLC). Here we characterized the TDE2 gene and determined its transcript levels in a panel of lung tumors, adjace...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Player A,Gillespie J,Fujii T,Fukuoka J,Dracheva T,Meerzaman D,Hong KM,Curran J,Attoh G,Travis W,Jen J

    更新日期:2003-11-01 00:00:00

  • FOXO transcription factors at the interface of metabolism and cancer.

    abstract::Diabetes refers to a group of metabolic diseases characterized by impaired insulin signalling and high blood glucose. A growing body of epidemiological evidence links diabetes to several types of cancer but the underlying molecular mechanisms are poorly understood. The signalling cascade connecting insulin and FOXO pr...

    journal_title:International journal of cancer

    pub_type: 杂志文章,评审


    authors: Link W,Fernandez-Marcos PJ

    更新日期:2017-12-15 00:00:00

  • Investigating Black-White differences in prostate cancer prognosis: A systematic review and meta-analysis.

    abstract::The case-fatality rate following a diagnosis of prostate cancer is higher for Black men compared to White men. How this elevated rate arises is uncertain, with differences in disease biology, presentation, treatment and comorbidity having been suggested. A systematic search was conducted for articles that reported eth...

    journal_title:International journal of cancer

    pub_type: 杂志文章,meta分析,评审


    authors: Evans S,Metcalfe C,Ibrahim F,Persad R,Ben-Shlomo Y

    更新日期:2008-07-15 00:00:00

  • Site-selective cyclic AMP analogues are antagonistic to estrogen stimulation of growth and proto-oncogene expression in human breast-cancer cells.

    abstract::Cyclic AMP (cAMP) analogues that selectively bind to either one of the two binding sites of cAMP-dependent protein kinase demonstrate a potent inhibition of the growth stimulated by estrogen in MCF-7 human breast-cancer cells in culture. The site-selective analogues, which are more potent activators of protein kinase ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Katsaros D,Ally S,Cho-Chung YS

    更新日期:1988-06-15 00:00:00

  • Trilaciclib prior to chemotherapy and atezolizumab in patients with newly diagnosed extensive-stage small cell lung cancer: A multicentre, randomised, double-blind, placebo-controlled Phase II trial.

    abstract::Trilaciclib is an intravenous CDK4/6 inhibitor administered prior to chemotherapy to preserve haematopoietic stem and progenitor cells and immune system function from chemotherapy-induced damage (myelopreservation). The effects of administering trilaciclib prior to carboplatin, etoposide and atezolizumab (E/P/A) were ...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Daniel D,Kuchava V,Bondarenko I,Ivashchuk O,Reddy S,Jaal J,Kudaba I,Hart L,Matitashvili A,Pritchett Y,Morris SR,Sorrentino JA,Antal JM,Goldschmidt J

    更新日期:2020-12-21 00:00:00

  • Nutrients and gastric cancer risk. A population-based case-control study in Sweden.

    abstract::A population-based case-control study of gastric cancer was conducted in areas with contrasting incidence rates in Sweden. Face-to-face interviews were carried out with 338 (74.1%) of all eligible cases and 679 (77.3%) of the selected controls. Consumption of selected nutrients during adolescence and 20 years prior to...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Hansson LE,Nyrén O,Bergström R,Wolk A,Lindgren A,Baron J,Adami HO

    更新日期:1994-06-01 00:00:00

  • CARD9 prevents lung cancer development by suppressing the expansion of myeloid-derived suppressor cells and IDO production.

    abstract::Caspase recruitment domain-containing protein 9 (CARD9) is an adaptor protein and highly expressed in myeloid cells. Our previous study demonstrates a critical protective effect of CARD9 in the development of colitis-associated colon cancer. Nevertheless, the effect of CARD9 in lung cancer remains unclear. Here, using...

    journal_title:International journal of cancer

    pub_type: 杂志文章


    authors: Qu J,Liu L,Xu Q,Ren J,Xu Z,Dou H,Shen S,Hou Y,Mou Y,Wang T

    更新日期:2019-10-15 00:00:00

  • Leukemia stem cells.

    abstract::Leukemia stem cells (LSCs) might originate from malignant transformation of normal hematopoietic stem cells (HSCs), or alternatively, of progenitors in which the acquired mutations have re-installed a dysregulated self-renewal program. LSCs are on top of a hierarchy and generate leukemia cells with more differentiated...

    journal_title:International journal of cancer

    pub_type: 杂志文章,评审


    authors: Buss EC,Ho AD

    更新日期:2011-11-15 00:00:00