Abstract：
:Improved biomarkers for prostate cancer (PC) risk stratification are urgently needed. Here, we aimed to develop a novel multimarker model for prediction of biochemical recurrence (BCR) after curatively intended radical prostatectomy (RP), based on minimally invasive sampling of blood and urine. We initially measured the levels of 45 selected miRNAs by RT-qPCR in exosome enriched cell-free urine samples collected prior to RP from 215 PC patients (Cohort 1, training). We trained a novel logistic regression model (pCaP), comprising five urine miRNAs (miR-151a-5p, miR-204-5p, miR-222-3p, miR-23b-3p and miR-331-3p) and serum prostate-specific antigen (PSA), which significantly predicted time to BCR in Cohort 1 (univariate Cox regression analysis: HR = 3.12, p < 0.001). Next, using the same exact numeric cutoff for dichotomization as trained in Cohort 1, we tested and successfully validated the prognostic potential of pCaP in two additional cohorts, including 199 (Cohort 2, HR = 2.24, p = 0.002) and 205 (Cohort 3, HR = 2.15, p = 0.004) RP patients, respectively. pCaP remained a significant predictor of BCR, also after adjustment for pathological T-stage, surgical margin status and Gleason grade group (p < 0.05 in multivariate Cox regression analysis: HR = 2.72, 1.94 and 1.83 for Cohorts 1, 2 and 3, respectively). Additionally, pCaP scores correlated positively with the established clinical risk stratification nomogram CAPRA in all three PC cohorts (Pearson's rho: 0.45, 0.39 and 0.44). Together, our results suggest that the minimally invasive pCaP model could potentially be used in the future to improve PC risk stratification and to guide more personalized treatment decisions. Further clinical validation studies are warranted.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Fredsøe J,Rasmussen AKI,Mouritzen P,Borre M,Ørntoft T,Sørensen KDdoi
10.1002/ijc.32296subject
Has Abstractpub_date
2019-11-01 00:00:00pages
2558-2567issue
9eissn
0020-7136issn
1097-0215journal_volume
145pub_type
杂志文章abstract：:Chemotherapy with taxanes such as paclitaxel (PTX) is a key component of triple negative breast cancer (TNBC) treatment. PTX is used in combination with other drugs in both the adjuvant setting and in advanced breast cancer. Because a proportion of patients respond poorly to PTX or relapse after its use, a greater und...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.30137
更新日期：2016-09-01 00:00:00
abstract：:Serum and tumor tissue from breast cancer patients were evaluated for the presence of alpha subunit of hCG, by means of the specific radioimmunoassay. Elevated serum levels occurred in 0/50 (0%) of normal female controls, 1/17 (6%) of patients with benign breast disease, 3/53 (6%) of patients with primary breast cance...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910250508
更新日期：1980-05-15 00:00:00
abstract：:In childhood acute lymphoblastic leukaemia there are large interpatient variations in levels of the apoptosis-regulating proteins Bax and Bcl-2, but the molecular basis for this variation is unknown. Point-mutations in bax have been reported in cell lines derived from haematological malignancies. Frameshift mutations,...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/(sici)1097-0215(19980619)79:3<273::aid-ijc
更新日期：1998-06-19 00:00:00
abstract：:Withaferin A (WFA) is purified from the plant Withania somnifera and inhibits the vimentin cytoskeleton. Vimentin overexpression in cancer correlates with metastatic disease, induction of epithelial to mesenchymal transition and reduced patient survival. As vimentin functions in cell motility, we wanted to test the hy...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.25938
更新日期：2011-12-01 00:00:00
abstract：:In a prospective cohort study of the health effects associated with prenatal Diethylstilbestrol (DES) exposure, DES was associated with an increased breast cancer risk after 40 years of age. It is unknown whether it is associated with greater mammographic density, which strongly predicts breast cancer risk. A cohort o...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.31524
更新日期：2018-09-15 00:00:00
abstract：:The sialosyl Lewis a antigen was identified in a ganglioside extract from a malignant human urothelial cell line (Hu 1703He) by fast atom bombardment mass spectrometry. The presence of this antigen in urothelial cell lines with varying tumorigenic properties was further studied using the 19-9 monoclonal antibody (MAb)...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910450635
更新日期：1990-06-15 00:00:00
abstract：:Female residents of six counties in Washington and Utah in whom a diagnosis of ovarian cancer was made during 1975-79 were interviewed concerning prior use of oral contraceptives. Interviews with a random sample of women drawn from these same counties were obtained for comparison. A smaller proportion of women with ep...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910280602
更新日期：1981-12-01 00:00:00
abstract：:Various angiogenesis-related self-molecules have been considered to be therapeutic targets. However, the direct use of self-molecules as vaccines is not recommended because of the inherent ability of the host to develop immune tolerance. Antigen 43 (Ag43) is a surface protein found in E. coli and contains an α and a β...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.28511
更新日期：2014-04-15 00:00:00
abstract：:Condylomata acuminata have been shown to increase the risk of anogenital cancers. However, previous studies have been of limited sample size and/or short follow-up duration, which prevent precise estimates of long-term excess risk, especially for specific cancer sites. We estimated the risk of specific cancers in a la...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.21892
更新日期：2006-08-15 00:00:00
abstract：:Smoking is indisputably linked to lung cancer, yet only a small fraction of smokers develops this disease. Although previously tobacco-derived carcinogens and enzyme polymorphisms have been identified to increase the risk for smokers, recent epidemiological data suggest even sex-specificity as a new and additional fac...
journal_title：International journal of cancer
pub_type： 杂志文章,评审
doi：10.1002/ijc.32716
更新日期：2020-05-01 00:00:00
abstract：:LAF-4, which encodes a nuclear protein with transactivation potential, is fused to the MLL gene in acute lymphoblastic leukemia (ALL). We identified LAF-4 as a gene that is transcriptionally deregulated in breast tumors and thus may have a pathological role in mammary tumorigenesis. In line with the previous finding t...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.20881
更新日期：2005-07-01 00:00:00
abstract：:Human colon cancer cells produce and secrete a variety of polypeptide growth factors. The functional role of these growth factors, however, is poorly understood. Though the secretion of epidermal growth factor (EGF)-like activity and EGF-related molecules by human colon cancer cells in culture has been reported, it is...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910520625
更新日期：1992-12-02 00:00:00
abstract：:Tumor cell motility is one of the rate-limiting steps of invasion, which defines progression toward a more malignant phenotype. Elevated expression of epidermal growth factor receptor (EGFR) in many cancers is associated with progression of superficial to invasive forms of the disease. The naturally occuring type III ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.11566
更新日期：2004-02-20 00:00:00
abstract：:Cervical carcinogenesis is a consequence of persistent infection with high-risk human papillomaviruses (HPVs). Recent studies indicate that HPV-transformed cells actively instruct their microenvironment to promote carcinogenesis. Here, we demonstrate that cervical cancer cells activate monocytes to produce their own C...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.28549
更新日期：2014-05-01 00:00:00
abstract：:The 26S proteasome, consisting of the 20S core and 19S regulatory complexes, regulates intracellular protein concentration through proteolytic degradation of targeted substrates. Composition of the 19S regulatory complex as well as posttranslational modifications of the 19S subunits can effectively regulate the activi...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.20261
更新日期：2004-09-01 00:00:00
abstract：:A recent clinical trial found a protective role of niacinamide, a derivative of niacin, against skin cancer recurrence. However, there is no epidemiologic study to assess the association between niacin intake and risk of skin cancer [basal cell carcinoma (BCC), squamous cell carcinoma (SCC) and melanoma]. We prospecti...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.30630
更新日期：2017-05-01 00:00:00
abstract：:Resistance to cisplatin-based chemotherapy is a major cause of treatment failure in human ovarian cancer. Wild-type TP53 status is often, but not always, associated with cisplatin sensitivity, suggesting that additional factors may be involved. Overexpression/activation of the phosphatidylinositol-3-kinase/Akt pathway...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.23086
更新日期：2008-02-01 00:00:00
abstract：:Understanding the reasons for nonparticipation in cancer screening may give clues about how to improve compliance. However, limited cooperation has hampered research on nonparticipant profiles. We took advantage of Sweden's comprehensive demographic and health care registers to investigate characteristics of all parti...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.23208
更新日期：2008-04-01 00:00:00
abstract：:The aim of this study was to assess the diagnostic performance of breast imaging in the diagnosis of breast cancer in a non-screening population. In a consecutive set of patients referred for mammography in one year, the results of palpation and radiological breast imaging were scored on a 5-point grading scale and li...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.1235
更新日期：2001-05-15 00:00:00
abstract：:We have analyzed the BRAF locus on chromosome 7q34 with microsatellites for allelic changes and exons 11 and 15 of the BRAF with sequencing for mutations in 50 kidney cancers including 20 papillary, 15 conventional and 15 chromophobe renal cell carcinomas (RCC). Allelic changes at the BRAF locus were seen in 16 of the...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.11305
更新日期：2003-10-10 00:00:00
abstract：:The identification of (gamma-glutamyl)polyamines in proteolytic digest of proteins from the cytosolic and particulate fractions of B16-F10 and B16-F10Lr6 cell lines, originating from a spontaneous tumor in C57BL/6 mice, indicates that polyamines are incorporated into melanoma cell proteins by transglutaminases (TGases...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910530515
更新日期：1993-03-12 00:00:00
abstract：:Ocular adnexal MALT lymphoma (OAML) is linked to Chlamydophila psittaci (Cp) infection. Viability and infectivity of Cp, demonstrated by growth in culture, has not been yet investigated in these patients. We conducted a single-center prospective case-control study to assess the prevalence, viability and infectivity of...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.23596
更新日期：2008-09-01 00:00:00
abstract：:Knowledge about the prevalence of human papillomavirus (HPV) on a population level is important. We conducted a large population-based study in Denmark to determine the overall and age-specific HPV prevalence, and HPV type distribution in women. Liquid-based cytology samples (SurePath) were collected consecutively. HP...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.23712
更新日期：2008-10-15 00:00:00
abstract：:With an increasing cancer rate worldwide, there is an urgent quest for the improvement of anticancer drugs. One of the main problems of present chemotherapy in treatment of tumor patients is the toxicity of drugs. Most of the existent anticancer drugs, unfortunately, attack also proliferating normal cells. In recent y...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.22883
更新日期：2007-10-15 00:00:00
abstract：:A specific chromosomal aberration was observed in 14 of 15 avian lymphoblastoid cell lines transformed with Marek's disease herpesvirus. This aberration, designated dup(1p)(p22-p23), appeared as an extra G-positive band and interband on the short arm of one chromosome I homolog. Using fluorescent in situ hybridization...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910540426
更新日期：1993-06-19 00:00:00
abstract：:We have examined, in proliferating neoplastic cells, the effect on DNA of EGTA (a chelator of Ca2+) and W7 (an inhibitor of calmodulin). The treatment results in release of single-stranded DNA fragments (2-10 kb) from pre-labelled HMW DNA. When DNA from synchronized neoplastic cells in S phase is examined, almost all ...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910370615
更新日期：1986-06-15 00:00:00
abstract：:Limited penetration of anticancer drugs through tumour tissue is an important factor that may limit therapeutic effects against solid tumours. Here we report studies of the penetration of radiolabelled methotrexate through multicellular layers (MCL) of murine EMT-6 and human MCF-7 cells grown on semiporous teflon memb...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/1097-0215(20010101)91:1<120::aid-ijc1021>3
更新日期：2001-01-01 00:00:00
abstract：:The incidence of testicular cancer was examined in the Nordic and Baltic countries, Poland and Germany by collaboration among 10 cancer registries. Population-based registers were used to analyze a total of 34,309 cases, diagnosed from the start of registration (varying from 1943 in Denmark to 1980 in Latvia and Lithu...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.2910590108
更新日期：1994-10-01 00:00:00
abstract：:Treatment of lung cancer is often performed with cone-down oblique beams to spare spinal cord and normal structures. However, there is no optimum technique to determine oblique beam angles when a CT simulation is not available. Impact of oblique beam angle was investigated in this study. Fifteen patients with centrall...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/1097-0215(20001220)90:6<359::aid-ijc8>3.0.
更新日期：2000-12-20 00:00:00
abstract：:It has become axiomatic that critical windows of susceptibility to genotoxins exist and that genetic damage in utero may be a trigger for later life cancers. Data supporting this critical window hypothesis are remarkably few. This study provides a quantitative bridge between DNA damage by the liver carcinogen aflatoxi...
journal_title：International journal of cancer
pub_type： 杂志文章
doi：10.1002/ijc.29102
更新日期：2015-03-15 00:00:00
