Abstract：
:The present study was designed to quantify the major cellular metabolites in human placentae and their changes due to the confounding effect of time and subsequent hypoxia during sample collection using magnetic resonance spectroscopy ((1)H-and (31)P-MRS). The absolute placental concentrations of lactate, glucose, major amino acids and cellular volume/osmo-regulators, glutathione, high-energy phosphates, fatty acids, phospholipids, triglycerols, and cholesterol are reported. There were no spatial differences in metabolism or protein expression throughout the placenta. The most significant temporal changes, due to the collection time (from 1 to 25 min after delivery), were increased concentrations of lactate (r=0.996, statistically significant P< 0.01 after 11 min) and decreased concentration of glucose and ATP (r=-0.963 and -0.97, respectively, P< 0.01 after 11 min). The placental samples from the later collection groups (16-24 min) had also significantly lower level of NAD(+) (r=-0.95, P< 0.01). Only the latest collection group (21-24 min) had increased lipid peroxidation and changes in lipid metabolites (P< 0.01). We conclude that the optimal window for collecting placental tissue without incurring metabolic artifacts due to hypoxic conditions is within 9 min of placental delivery.
journal_name
Placentajournal_title
Placentaauthors
Serkova N,Bendrick-Peart J,Alexander B,Tissot van Patot MCdoi
10.1053/plac.2002.0908keywords:
subject
Has Abstractpub_date
2003-02-01 00:00:00pages
227-35issue
2-3eissn
0143-4004issn
1532-3102pii
S0143400402909082journal_volume
24pub_type
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