Interaction of pentobarbital with gabaergic drugs acting on the Cl(-)-ATPase activity of the plasma membranes from bream brain (Abramis brama L.).

Abstract:

:The present study was designed to investigate the role of the interaction of pentobarbital with gamma-aminobutyric acid (GABA)ergic drugs acting on the Cl(-)-adenosine triphosphate (ATP)ase activity of the plasma membranes fraction of bream brain. The preincubation and then incubation of the membranes with pentobarbital as well as with other GABAergic ligands was conducted at physiologic pH (7.4), i.e. at the condition where the Cl(-)-ATPase activity is not detected. Pentobarbital (1-100 microM) induces Cl(-)-ATPase activity, however at high concentration (1,000 microM) no effect of the ligand was found. In addition pentobarbital (50 microM) enhances the effect of low concentration of GABA (1 microM) on the Cl(-)-ATPase activity, but inhibits the action of high concentration of GABA (100 microM) on the enzyme. Whereas no activating effect of pentobarbital in the presence of baclofen (1 microM) was found. The blocker of GABA(A)-receptors, picrotoxin (50 microM) and bicuculline (5 microM) eliminated the action of pentobarbital on the enzyme. The present results provide evidence for the first time that at physiologic pH in incubation medium the interaction of pentobarbital with GABAergic drugs on the Cl(-)-ATPase activity is similar to the effects of these ligands on the GABA(A)-receptor.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Menzikov S,Menzikova O

doi

10.1016/s0304-3940(02)01076-5

keywords:

subject

Has Abstract

pub_date

2002-12-16 00:00:00

pages

161-4

issue

3

eissn

0304-3940

issn

1872-7972

pii

S0304394002010765

journal_volume

334

pub_type

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