Abstract:
:14-3-3 proteins are involved in signalling processes in neuronal cells. Using isoform-specific antibodies we have examined the variation in 14-3-3 isoform neurolocation in normal and scrapie-infected murine brain and show that in defined areas of the brain there are significant changes associated with the pathology of the disease process. The appearance of 14-3-3 proteins in the cerebrospinal fluid (CSF) is a consequence of neuronal disease and the detection of specific isoforms of the 14-3-3 proteins in the CSF is characteristic of some neurodegenerative diseases. In this study, monitoring specifically for the gamma 14-3-3 isoform in the CSF by both Western-blot analysis and ELISA we can show a level of correlation between the assays.
journal_name
Biochem Soc Transjournal_title
Biochemical Society transactionsauthors
Baxter HC,Fraser JR,Liu WG,Forster JL,Clokie S,Steinacker P,Otto M,Bahn E,Wiltfang J,Aitken Adoi
10.1042/bst0300387keywords:
subject
Has Abstractpub_date
2002-08-01 00:00:00pages
387-91issue
4eissn
0300-5127issn
1470-8752journal_volume
30pub_type
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