The ProteinChip Biomarker System from Ciphergen Biosystems: a novel proteomics platform for rapid biomarker discovery and validation.


:Protein biochips are emerging in two distinct formats. The first involves high-density immobilized arrays of recognition molecules (e.g. antibodies), where target binding is monitored indirectly (e.g. via fluorescence). This technology is in its infancy, being limited by the availability of suitable binding molecules that can cope effectively with protein diversity. The second format involves the capture of proteins by biochemical or intermolecular interaction, coupled with direct detection by MS. This technology is available as the ProteinChip Biomarker System. ProteinChip technology uses surface-enhanced laser desorption/ionization processes to analyse proteins directly from biological samples. Chromatographic surfaces are placed on to ProteinChip Arrays and used to capture subclasses of proteins, dependent on their physical properties. Time-of-flight MS then assigns native molecular masses to the captured proteins. Reproducible protein profiles can be generated from crude biological fluids (e.g. cell lysates, urine or serum). The technology is being applied to a wide range of disciplines, from plant sciences to cancer research, and will be reviewed here.


Biochem Soc Trans


Chapman K





Has Abstract


2002-04-01 00:00:00












  • Ion translocation by the Escherichia coli NADH:ubiquinone oxidoreductase (complex I).

    abstract::The energy-converting NADH:ubiquinone oxidoreductase, also known as respiratory complex I, couples the transfer of electrons from NADH to ubiquinone with the translocation of ions across the membrane. It was assumed that the complex exclusively works as a proton pump. Recently, it has been proposed that complex I from...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Friedrich T,Stolpe S,Schneider D,Barquera B,Hellwig P

    更新日期:2005-08-01 00:00:00

  • Structural implications for the transformation of the Bacillus thuringiensis delta-endotoxins from water-soluble to membrane-inserted forms.

    abstract::Crystal structures combined with biochemical data show that the delta-endotoxins from Bacillus thuringiensis are structurally poised towards large-scale, irreversible conformational changes that transform them from the soluble protein bound at the cell surface into a membrane-embedded form causing lysis of susceptible...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Li J,Derbyshire DJ,Promdonkoy B,Ellar DJ

    更新日期:2001-08-01 00:00:00

  • Beta galactosidases in Arabidopsis and tomato - a mini review.

    abstract::Beta galactosidases (BGALs) are glycosyl hydrolases that remove terminal β-D-galactosyl residues from β-D-galactosides. There are 17 predicted BGAL genes in the genomes of both Arabidopsis (BGAL1-17) and tomato (TBG1-17). All tested BGALs have BGAL activity but their distinct expression profiles and ancient phylogenet...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Chandrasekar B,van der Hoorn RA

    更新日期:2016-02-01 00:00:00

  • Role of GABAA receptors in cognition.

    abstract::Complex brains have developed specialized mechanisms for the grouping of principal cells into temporal coalitions of local or distant networks: the inhibitory interneuron 'clocking' networks. They consist of GABAergic (where GABA is gamma-aminobutyric acid) interneurons of a rich diversity. In cortical circuits, these...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Möhler H

    更新日期:2009-12-01 00:00:00

  • Mesophyll conductance: the leaf corridors for photosynthesis.

    abstract::Besides stomata, the photosynthetic CO2 pathway also involves the transport of CO2 from the sub-stomatal air spaces inside to the carboxylation sites in the chloroplast stroma, where Rubisco is located. This pathway is far to be a simple and direct way, formed by series of consecutive barriers that the CO2 should cros...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Gago J,Daloso DM,Carriquí M,Nadal M,Morales M,Araújo WL,Nunes-Nesi A,Flexas J

    更新日期:2020-04-29 00:00:00

  • Plant copper chaperones.

    abstract::Copper chaperones, soluble copper-binding proteins, are essential for ensuring proper distribution of copper to cellular compartments and to proteins requiring copper prosthetic groups. They are found in all eukaryotic organisms. Orthologues of the three copper chaperones characterized in yeast, ATX1, CCS and COX17, a...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Wintz H,Vulpe C

    更新日期:2002-08-01 00:00:00

  • The third extracellular loop of G-protein-coupled receptors: more than just a linker between two important transmembrane helices.

    abstract::GPCRs (G-protein-coupled receptors) are a large family of structurally related proteins, which mediate their effects by coupling with G-proteins. Despite responding to a range of very diverse stimuli, these receptors exhibit a conserved tertiary structure comprising a bundle of seven TM (transmembrane) helices linked ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Lawson Z,Wheatley M

    更新日期:2004-12-01 00:00:00

  • Mechanisms of microbial escape from phagocyte killing.

    abstract::Phagocytosis and phagosome maturation are crucial processes in biology. Phagocytosis and the subsequent digestion of phagocytosed particles occur across a huge diversity of eukaryotes and can be achieved by many different cells within one organism. In parallel, diverse groups of pathogens have evolved mechanisms to av...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Smith LM,May RC

    更新日期:2013-04-01 00:00:00

  • Prelamin A-mediated nuclear envelope dynamics in normal and laminopathic cells.

    abstract::Prelamin A is the precursor protein of lamin A, a major constituent of the nuclear lamina in higher eukaryotes. Increasing attention to prelamin A processing and function has been given after the discovery, from 2002 to 2004, of diseases caused by prelamin A accumulation. These diseases, belonging to the group of lami...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Lattanzi G

    更新日期:2011-12-01 00:00:00

  • Rho-ROCK signaling regulates tumor-microenvironment interactions.

    abstract::Reciprocal biochemical and biophysical interactions between tumor cells, stromal cells and the extracellular matrix (ECM) result in a unique tumor microenvironment that determines disease outcome. The cellular component of the tumor microenvironment contributes to tumor growth by providing nutrients, assisting in the ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Johan MZ,Samuel MS

    更新日期:2019-02-28 00:00:00

  • Structural predictions of the functions of membrane proteins from HDX-MS.

    abstract::HDX-MS has emerged as a powerful tool to interrogate the structure and dynamics of proteins and their complexes. Recent advances in the methodology and instrumentation have enabled the application of HDX-MS to membrane proteins. Such targets are challenging to investigate with conventional strategies. Developing new t...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Lau AM,Jia R,Bradshaw RT,Politis A

    更新日期:2020-06-29 00:00:00

  • Polyunsaturated fatty acid-specific elongation enzymes.

    abstract::We have isolated a novel gene (GLELO) from Mortierella alpina and its homologue (CEELO1) from Caenorhabditis elegans and demonstrate the involvement of their encoded proteins in the elongation of C(18) polyunsaturated fatty acids. ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Das T,Thurmond JM,Bobik E,Leonard AE,Parker-Barnes JM,Huang YS,Mukerji P

    更新日期:2000-12-01 00:00:00

  • Molecular characterization of substrate-binding sites in the glutamate transporter family.

    abstract::Glutamate transporters are essential for terminating synaptic excitation and for maintaining extracellular glutamate concentrations below neurotoxic levels. These transporters also mediate a thermodynamically uncoupled chloride flux that is activated by two of the molecules that they transport - sodium and glutamate. ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Kanner BI,Kavanaugh MP,Bendahan A

    更新日期:2001-11-01 00:00:00

  • Solution structures of complement components by X-ray and neutron scattering and analytical ultracentrifugation.

    abstract::The short consensus/complement repeat (SCR) domain (also known as the complement control protein domain) is the most abundant domain type in the complement system. Crystal and NMR structures for proteins that contain single and multiple SCR domains have now been published. These contain inter-SCR linkers of between th...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Perkins SJ,Gilbert HE,Aslam M,Hannan J,Holers VM,Goodship TH

    更新日期:2002-11-01 00:00:00

  • Alkalithermophiles.

    abstract::Alkalithermophiles are an exciting subset of extremophilic organisms and represent extremophiles that are adapted to two extreme conditions, i.e. to a combination of alkaline and thermobiotic growth conditions. Among the anaerobic alkalithermophiles are representatives of both Bacteria and Archaea within a wide variet...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Wiegel J,Kevbrin VV

    更新日期:2004-04-01 00:00:00

  • Opportunities for bioprocess monitoring using FRET biosensors.

    abstract::Bioprocess monitoring is used to track the progress of a cell culture and ensure that the product quality is maintained. Current schemes for monitoring metabolism rely on offline measurements of samples of the extracellular medium. However, in the era of synthetic biology, it is now possible to design and implement bi...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Constantinou A,Polizzi KM

    更新日期:2013-10-01 00:00:00

  • Lipoxygenase pathway in tulip: biosynthesis of ketols.

    abstract::The metabolism in vitro of [1-(14)C]linoleate, [1-(14)C]linolenate and their 9(S)-hydroperoxides in tulip (Tulipa gesneriana) was found to be under the control of 9-lipoxygenase and allene oxide synthase, and directed towards alpha-ketol, gamma-ketol and the novel compound (12Z)-10-oxo-11-hydroxy-12-octadecadienoic ac...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Grechkin AN,Mukhtarova LS,Hamberg M

    更新日期:2000-12-01 00:00:00

  • Recycling domains in plant cell morphogenesis: small GTPase effectors, plasma membrane signalling and the exocyst.

    abstract::The Rho/Rop small GTPase regulatory module is central for initiating exocytotically ACDs (active cortical domains) in plant cell cortex, and a growing array of Rop regulators and effectors are being discovered in plants. Structural membrane phospholipids are important constituents of cells as well as signals, and phos...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Zárský V,Potocký M

    更新日期:2010-04-01 00:00:00

  • Role of the phosphoinositide 3-kinase pathway in mouse embryonic stem (ES) cells.

    abstract::Mouse ES (embryonic stem) cells maintain pluripotency with robust proliferation in vitro. ES cells share some similarities with cancer cells, such as anchorage-independent growth, loss of contact inhibition and tumour formation. After differentiation, ES cells lose pluripotency and tumorigenicity. Recent studies showe...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Takahashi K,Murakami M,Yamanaka S

    更新日期:2005-12-01 00:00:00

  • LRRK2 links genetic and sporadic Parkinson's disease.

    abstract::The past two decades in research has revealed the importance of leucine-rich repeat kinase 2 (LRRK2) in both monogenic and sporadic forms of Parkinson's disease (PD). In families, mutations in LRRK2 can cause PD with age-dependent but variable penetrance and genome-wide association studies have found variants of the g...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Kluss JH,Mamais A,Cookson MR

    更新日期:2019-04-30 00:00:00

  • The quest for Factor VIIa exosite inhibitors.

    abstract::Coagulation proteases are involved in a highly orchestrated proteolytic cascade which is essential for haemostasis and blood clotting. In particular, the initiator of the coagulation cascade, Factor VIIa, binds to its cofactor, tissue factor, and its substrate, Factor X, via exosite interactions to form a ternary cata...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Amour A,Hutchinson J,Ruiz Avendaño AM,Ratcliffe S,Alvarez E,Martin J,Toomey JR,Senger S,Wolfendale M,Mooney C

    更新日期:2007-06-01 00:00:00

  • Expression, purification and properties of multidrug efflux proteins.

    abstract::A general strategy is described for the amplified expression, purification and characterization in Escherichia coli of multidrug efflux proteins from Staphylococcus aureus, Bacillus subtilis, Methanococcus janaschii and E. coli. They all catalyse drug/H(+) antiport of substrates such as quinolones and ethidium and exe...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Henderson PJ,Hoyle CK,Ward A

    更新日期:2000-01-01 00:00:00

  • Biomolecular complex viewed by dynamic nuclear polarization solid-state NMR spectroscopy.

    abstract::Solid-state nuclear magnetic resonance (ssNMR) is an indispensable tool for elucidating the structure and dynamics of insoluble and non-crystalline biomolecules. The recent advances in the sensitivity-enhancing technique magic-angle spinning dynamic nuclear polarization (MAS-DNP) have substantially expanded the territ...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Chakraborty A,Deligey F,Quach J,Mentink-Vigier F,Wang P,Wang T

    更新日期:2020-06-30 00:00:00

  • Perspectives on neuroprotective stroke therapy.

    abstract::After years of setbacks, the perspective of neuroprotective stroke therapy has revived in light of recent study results. We outline in this review how a neuroprotective candidate drug should be developed, beginning with a thorough preclinical evaluation according to the STAIR (Stroke Therapy Academic Industry Roundtab...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Schäbitz WR,Fisher M

    更新日期:2006-12-01 00:00:00

  • Presenilins: how much more than γ-secretase?!

    abstract::AD (Alzheimer's disease) is a neurodegenerative disease characterized by a gradual loss of neurons and the accumulation of neurotoxic Aβ (amyloid β-peptide) and hyperphosphorylated tau. The discovery of mutations in three genes, PSEN1 (presenilin 1), PSEN2 (presenilin 2) and APP (amyloid precursor protein), in patient...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Coen K,Annaert W

    更新日期:2010-12-01 00:00:00

  • Relating SMCHD1 structure to its function in epigenetic silencing.

    abstract::The structural maintenance of chromosomes hinge domain containing protein 1 (SMCHD1) is a large multidomain protein involved in epigenetic gene silencing. Variations in the SMCHD1 gene are associated with two debilitating human disorders, facioscapulohumeral muscular dystrophy (FSHD) and Bosma arhinia microphthalmia s...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Gurzau AD,Blewitt ME,Czabotar PE,Murphy JM,Birkinshaw RW

    更新日期:2020-08-28 00:00:00

  • Structural dynamics of P-type ATPase ion pumps.

    abstract::P-type ATPases transport ions across biological membranes against concentration gradients and are essential for all cells. They use the energy from ATP hydrolysis to propel large intramolecular movements, which drive vectorial transport of ions. Tight coordination of the motions of the pump is required to couple the t...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Dyla M,Basse Hansen S,Nissen P,Kjaergaard M

    更新日期:2019-10-31 00:00:00

  • Coupling the dynamics of two actin networks--new views on the mechanics of cell protrusion.

    abstract::We study how mechanical forces integrate spatially and temporally with regulatory signals at the leading edge of migrating cells. To probe the dynamics of this system, we developed quantitative fluorescent speckle microscopy, which maps out actin cytoskeleton transport, assembly and disassembly with high spatial resol...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Danuser G

    更新日期:2005-12-01 00:00:00

  • TPC: the NAADP discovery channel?

    abstract::The Ca2+-mobilizing second messenger, NAADP (nicotinic acid adenine dinucleotide phosphate), has been with us for nearly 20 years and yet we still cannot fully agree on the identity of its target Ca2+-release channel. In spite of some recent robust challenges to the idea that two-pore channels (TPCs) represent the elu...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章,评审


    authors: Morgan AJ,Davis LC,Ruas M,Galione A

    更新日期:2015-06-01 00:00:00

  • A new approach for the investigation of reperfusion-related brain injury.

    abstract::Effective stroke therapies require recanalization of occluded cerebral blood vessels; however, early reperfusion can cause BBB (blood-brain barrier) injury, leading to cerebral oedema and/or devastating brain haemorrhage. These complications of early reperfusion, which result from excess production of ROS (reactive ox...

    journal_title:Biochemical Society transactions

    pub_type: 杂志文章


    authors: Maier CM,Hsieh L,Crandall T,Narasimhan P,Chan PH

    更新日期:2006-12-01 00:00:00