Abstract:
:Studies to clarify the mechanisms of apoptosis in host cells, A31 (BALB/3T3 clone A31 fibroblasts), caused by two intracellular protozoan parasites, Toxoplasma gondii and Neospora caninum, were carried out in an in vitro system. The viability of N. caninum-infected cells was significantly reduced following treatment with mouse interferon (IFN)-gamma. By contrast, mouse IFN-gamma treatment had no significant effect on the induction of apoptosis in T. gondii-infected cells. Apoptosis of N. caninum-infected and mouse IFN-gamma-treated cells was shown to be associated with increased DNA fragmentation, and increased caspase-3 and -8 activity, and the administration of caspase-3 and -8 inhibitors inhibited cell death. FasL expression was clearly induced by N. caninum-infection and IFN-gamma treatment compared to the T. gondii-infected cells and the uninfected control with or without IFN-gamma treatment. The reduction in host-cell viability was prevented with the addition of antimouse FasL monoclonal antibody (mAb). Moreover, TUNEL analyses indicated that apoptosis was induced by the treatment with Fas mAb in both T. gondii and N. caninum-infected cells. These results suggest that the Fas/FasL pathway may play a crucial role in the induction of apoptosis in N. caninum- and T. gondii-infected cells mediated by IFN-gamma.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Nishikawa Y,Makala L,Otsuka H,Mikami T,Nagasawa Hdoi
10.1046/j.1365-3024.2002.00476.xkeywords:
subject
Has Abstractpub_date
2002-07-01 00:00:00pages
347-54issue
7eissn
0141-9838issn
1365-3024pii
476journal_volume
24pub_type
杂志文章abstract::Impairment of T cell proliferation in mice infected with the pleomorphic Trypanosoma brucei AnTat 1.1 E clone was found not to be related to a depletion of T cells or to an absence of functional accessory cells, but rather to an active suppression of interleukin 2 (IL-2) production. Lymph node cells derived from infec...
journal_title:Parasite immunology
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journal_title:Parasite immunology
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pub_type: 杂志文章
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更新日期:1988-01-01 00:00:00
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pub_type: 杂志文章,评审
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pub_type: 杂志文章
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1993-03-01 00:00:00
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更新日期:2012-12-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1991-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2015-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-05-01 00:00:00