Abstract:
:Leishmania enter macrophages through receptor-mediated phagocytosis and survive the harsh environment of a phagolysosome. Here, we investigated the interaction between mannose receptor (MR), Toll-like receptor 2 (TLR2), and Leishmania, and the subsequent impact on phagosome maturation. Leishmania parasites are able to delay phagosome maturation, not reaching full maturation until 5 hours post-engulfment. Here, maturation of Leishmania major- and Leishmania donovani-containing phagosomes proceeded as expected in the WT macrophages becoming LAMP1 positive by 6 hours. Interestingly, MR-/- macrophages become LAMP1 positive by ~2 hours and ~4 hours post-infection Leishmania-containing phagosomes lost LAMP1 expression and gained the early marker EEA1. LAMP1 expression was again observed by 6 hours. Leishmania LPG was essential for the delay in both WT and MR-/- macrophages but was not essential for the early maturation (2 hours) observed in MR-/- macrophages. Serum opsonization of Leishmania prior to infection induced identical phagosome maturation patterns in WT and MR-/- macrophages. In the absence of MyD88 or TLR2 on macrophages, Leishmania phagosomes matured significantly faster, becoming LAMP1 positive by ~1-2 hours. These studies add to the knowledge that phagosome maturation is influenced by multiple receptor-ligand interactions and signalling pathways.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Polando RE,Jones BC,Ricardo C,Whitcomb J,Ballhorn W,McDowell MAdoi
10.1111/pim.12521subject
Has Abstractpub_date
2018-04-01 00:00:00pages
e12521issue
4eissn
0141-9838issn
1365-3024journal_volume
40pub_type
杂志文章abstract::The role of MHC class II in the presentation of Heligmosomoides polygyrus antigens has been investigated, using a number of T cell hybridomas produced in A and E positive and negative mice. By using fixed and irradiated antigen presenting cells (APC), further evidence has emerged, to support earlier data, that there c...
journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1996-09-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1994.tb00320.x
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journal_title:Parasite immunology
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1995.tb01018.x
更新日期:1995-03-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1984.tb00790.x
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1990.tb00951.x
更新日期:1990-05-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1998.00127.x
更新日期:1998-02-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1982.tb00439.x
更新日期:1982-07-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:1981-10-01 00:00:00
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journal_title:Parasite immunology
pub_type: 临床试验,杂志文章
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更新日期:2005-04-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2007.00949.x
更新日期:2007-06-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.2002.00461.x
更新日期:2002-05-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1994.tb00299.x
更新日期:1994-01-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1991.tb00548.x
更新日期:1991-09-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12434
更新日期:2017-07-01 00:00:00
abstract::The incidence of both type 1 (T1D) and type 2 diabetes (T2D) is drastically increasing, and it is predicted that the global prevalence of diabetes will reach almost 600 million cases by 2035. Even though the pathogenesis of both types of diabetes is distinct, the immune system is actively involved in both forms of the...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12401
更新日期:2017-05-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1990.tb00936.x
更新日期:1990-01-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1999.00201.x
更新日期:1999-02-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1996.d01-1.x
更新日期:1996-01-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
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journal_title:Parasite immunology
pub_type: 杂志文章
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更新日期:2001-06-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12686
更新日期:2020-02-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1982.tb00437.x
更新日期:1982-07-01 00:00:00
abstract::The C57BL/6 mouse strain is resistant to Leishmania (L.) major infection and, unlike susceptible BALB/c, develops small self-healing cutaneous lesions. The specific antibody responses of C57BL/6 and BALB/c mice were previously characterized by the predominance of IgG2a ('resistant' isotype associated with Th1) and IgG...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12223
更新日期:2015-10-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1996.d01-112.x
更新日期:1996-07-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1996.d01-17.x
更新日期:1996-10-01 00:00:00
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journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12281
更新日期:2015-12-01 00:00:00
abstract::Lymphoid cells from genetically-susceptible BALB/c mice immunized against a glycoconjugate of the protozoan parasite, Leishmania major, promote chronic cutaneous disease in BALB/c nude mice. This cell population therefore differs from cells harvested from non-immunized BALB/c mice that are known to be potent mediators...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1986.tb01037.x
更新日期:1986-05-01 00:00:00
abstract::Low- and high-dose infections with the murine large intestinal nematode Trichuris muris are associated with induction of adaptive Th1 and Th2 responses, respectively, in mesenteric lymph nodes (MLN). Classical dendritic cells (cDC) accumulate in the large intestinal mucosa and MLN upon T. muris infection, yet their ro...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12458
更新日期:2017-10-01 00:00:00
abstract::A recent working group convened by the World Health Organization recommended that time to first or only episode of clinical malaria should be used to evaluate vaccine efficacy in phase III trials. However, calculating vaccine efficacy based on this endpoint misses important aspects of malaria disease and transmission....
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-3024.2009.01144.x
更新日期:2009-09-01 00:00:00