Abstract:
:Previous work has shown that a protein extract enriched for cysteine protease activity (TSBP) prepared from adult Haemonchus contortus using thiol sepharose affinity chromatography confers substantial protection against a single challenge infection. TSBP comprised proteases and other proteins. Here, TSBP were further fractionated using anion-exchange chromatography and fractions pooled on the basis of containing (1) protease activity, (2) a prominent non-protease peptide and (3) material which did not bind to the column. A protection trial showed that only the protease-enriched material conferred protective immunity and this was comparable to that observed in a TSBP-immunized positive control group. Immunization stimulated a marked IgG response with the IgG2 isotype predominating.
journal_name
Parasite Immunoljournal_title
Parasite immunologyauthors
Knox DP,Smith SK,Redmond DL,Smith WDdoi
10.1111/j.1365-3024.2005.00750.xkeywords:
subject
Has Abstractpub_date
2005-04-01 00:00:00pages
121-6issue
4eissn
0141-9838issn
1365-3024pii
PIM750journal_volume
27pub_type
临床试验,杂志文章abstract::Immunity to Schistosoma mansoni in the mouse was induced by vaccination with adult worm surface membrane (mb-S). Of several adjuvants tested, including Freund's, BCG and alum, 50 micrograms of saponin per mouse given subcutaneously with the antigen was the easiest to administer, and gave consistent protection, approac...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1989.tb00669.x
更新日期:1989-07-01 00:00:00
abstract::An essential element for continuing transmission of Plasmodium falciparum is the availability of mature gametocytes in human peripheral circulation for uptake by mosquitoes. Natural immune responses to circulating gametocytes may play a role in reducing transmission from humans to mosquitoes. Here, antibody recognitio...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12323
更新日期:2016-06-01 00:00:00
abstract::A monoclonal antibody (NIM-M7) has been prepared which reacts with a major surface antigen of adult males and females of Trichinella spiralis. This specificity is only demonstrable when the antigen is liberated by detergent solubilization of surface-labelled worms. When reacted with living adults, on the other hand, N...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1986.tb00874.x
更新日期:1986-11-01 00:00:00
abstract::Malaria parasites have a complex, multistage life cycle and there is a widely held view that each stage displays a distinct set of antigens presented to the immune system. Yet, molecular analysis of malaria parasites suggests that many putative antigenic targets are shared amongst the different stages. The specificiti...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12434
更新日期:2017-07-01 00:00:00
abstract::Visceral leishmaniasis (VL) or Kala azar (KA) is a systemic disease caused by the parasites of the Leishmania donovani complex. Control measures rely on treatment with antileishmanial agents, however, fraught with problems such as toxicity or drug resistance. The incidence rate is on the rise for children, for reasons...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2008.01036.x
更新日期:2008-08-01 00:00:00
abstract::A model for the in vitro infection of ovine cells with Toxoplasma gondii tachyzoites has been developed and used to investigate the effect of treatment with ovine recombinant interferon-gamma (ov.rIFN gamma) on parasite replication. Treatment of both alveolar macrophages and fibroblast cells either 24 h pre-infection ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1993.tb00641.x
更新日期:1993-09-01 00:00:00
abstract::TSOL18 is a recombinant protein that has been shown in repeated experimental trials to be capable of protecting pigs against challenge infection with the cestode parasite Taenia solium. Antibodies raised by the vaccine are capable of killing the parasite in an in vitro culture and it is believed that antibody and comp...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2009.01197.x
更新日期:2010-06-01 00:00:00
abstract::A comparison of Plasmodium chabaudi AS infection in BALB/c and BALB/c IgM-deficient mice demonstrated a protective role for IgM during infection. IgM-/- mice, unlike microMT mice, display competent B cell humoral immune responses. Increased susceptibility of IgM-/- mice was demonstrated by increased mortality, an adva...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.2005.00760.x
更新日期:2005-05-01 00:00:00
abstract::We have cloned the cDNA of TBA-1, the Nematode polyprotein allergen (NPA) of Toxocara canis and found it to be most similar to ABA-1, the Ascaris NPA, on the basis of amino acid sequence. We could study the antigenic properties of an E-coli synthesized fusion protein prepared with the cloned gene since no glycosylatio...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1998.00160.x
更新日期:1998-08-01 00:00:00
abstract::The variant surface glycoprotein (VSG) coat of African trypanosomes exhibits immunobiological functions distinct from its prominent role as a variant surface antigen. In order to address questions regarding immune stealth effects of VSG switch-variant coats, and the innate immune system activating effects of shed VSG ...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-3024.2007.01003.x
更新日期:2008-04-01 00:00:00
abstract::The effect of the humoral immune response on the weight and fecundity of the hookworm Necator americanus was examined in an endemically-infected human population. There was a highly significant negative correlation between total IgE levels and parasite weight and fecundity, after controlling for any effects of host ag...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1995.tb00968.x
更新日期:1995-02-01 00:00:00
abstract::The protein-energy malnutrition (PEM) and visceral leishmaniasis (VL) are important problems of public health, which affect millions of people worldwide. Currently, it has been accepted that the immunity or susceptibility to infect-parasitic diseases are directly related to the nutritional status of the host. However,...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-3024.2009.01117.x
更新日期:2009-10-01 00:00:00
abstract::Two monoclonal antibodies against the surface of S. mansoni schistosomula were found to confer significant passive protection to mice (M7B3A, range 28-70%; M22H12C, range 14-58%). No additive effect was observed when both were transferred together. Neither McAb bound to the cercarial surface but both bound to the surf...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1986.tb00836.x
更新日期:1986-01-01 00:00:00
abstract::The Bacillus thuringiensis Cry1Ac protoxin (pCry1Ac) is a promising mucosal immunogen and adjuvant that induces protective immunity against Naegleria fowleri and malaria infection models. We determined whether pCry1Ac acted as a protective adjuvant against infection with Taenia crassiceps. BALB/C mice were thrice i.p....
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12103
更新日期:2014-06-01 00:00:00
abstract::Serum from cats (Felis catus) that were repeatedly infected with Brugia pahangi and had become amicrofilaraemic (mf-ve) was injected intravenously into microfilaraemic (mf+ve) cats. If more than 1 microliter of immune serum per 1000 mf was injected, microfilarial counts fell dramatically within minutes and, in some ca...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1996.d01-50.x
更新日期:1996-02-01 00:00:00
abstract::There is good evidence for costs to both the uses of immune defences and their development and maintenance. The optimal defence will be a balance of these costs with the risk of infection and the virulence of the disease. It is therefore clear that the life-history characteristics of both host and parasite will impact...
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/pim.12055
更新日期:2013-11-01 00:00:00
abstract::After several in vitro treatments of a virulent population of Leishmania major with the mutagen, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), five clones (vir-) were obtained that did not produce cutaneous lesions after subcutaneous injection of 10(6) promastigotes. All the control clones (vir+) obtained from the non-...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1987.tb00490.x
更新日期:1987-01-01 00:00:00
abstract::A pilot safety and immunogenicity trial of the malaria vaccine SPf66 was undertaken in The Gambia in 1993. One hundred and fifty infants aged 6-11 months were immunized with either 0.5 mg or 1.0 mg of SPf66 produced either in Colombia or in the USA or with a control vaccine. Children who received SPf66 experienced mor...
journal_title:Parasite immunology
pub_type: 临床试验,杂志文章
doi:10.1046/j.1365-3024.1997.d01-171.x
更新日期:1997-12-01 00:00:00
abstract::The previously observed MHC-restriction of the antibody response to an 86kDa S. mansoni antigen has been investigated in more detail. The I-A locus of the H-2 complex has been implicated as conferring responder or non-responder status on mice expressing the k and b alleles respectively. Inheritance of responsiveness w...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1991.tb00281.x
更新日期:1991-05-01 00:00:00
abstract::Helper T cell cytokine and antibody responses were investigated in mice after infection with Babesia microti (King strain). Infection of CBA mice with 106 parasitized erythrocytes resulted in the development of a transitory high parasitaemia which peaked 14 days post infection (DPI), and was resolved at 24 DPI. Th1 re...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.2000.00279.x
更新日期:2000-02-01 00:00:00
abstract::We have previously reported the usefulness of a 26-28 kDa coproantigen of Fasciola hepatica for diagnosis of infection. In this study, the 26-28 kDa coproantigen was biochemically characterized with the aid of monoclonal antibodies (MoAb) in an effort to better understand the biology of the antigen. Differential stain...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1999.00191.x
更新日期:1999-06-01 00:00:00
abstract::Humoral immunity develops in the spleen during blood-stage Plasmodium infection. This elicits parasite-specific IgM and IgG, which control parasites and protect against malaria. Studies in mice have elucidated cells and molecules driving humoral immunity to Plasmodium, including CD4+ T cells, B cells, interleukin (IL)...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12455
更新日期:2017-10-01 00:00:00
abstract::Haemonchus contortus is an economically important parasite that survives the host defense system by modulating the immune response. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is secreted by the parasite and the host responds by producing anti-enzyme antibodies. The enzyme inhibits complement cascade, an arm of t...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12611
更新日期:2019-02-01 00:00:00
abstract::Immunization of mammals with irradiated malaria sporozoites protects from a subsequent contact with the parasite. Protective immunity is directed against the pre-erythrocytic stages of the parasite, sporozoites and liver stages. Specific antibodies neutralize part of the infectious sporozoites infected by the mosquito...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.1365-3024.1997.d01-190.x
更新日期:1997-03-01 00:00:00
abstract::A recent working group convened by the World Health Organization recommended that time to first or only episode of clinical malaria should be used to evaluate vaccine efficacy in phase III trials. However, calculating vaccine efficacy based on this endpoint misses important aspects of malaria disease and transmission....
journal_title:Parasite immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-3024.2009.01144.x
更新日期:2009-09-01 00:00:00
abstract::The development of a malaria vaccine is a priority for improved and sustained malaria control. Optimal use of a vaccine in Africa will only be achieved if it can be delivered through the Expanded Programme of Immunization (EPI). We have completed a trial of the peptide vaccine SPf66 in Tanzanian infants, given alongsi...
journal_title:Parasite immunology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1046/j.1365-3024.2000.00322.x
更新日期:2000-09-01 00:00:00
abstract::The p190 protein (also called MSA1 or MSP1) of the asexual blood stage forms of Plasmodium falciparum, a human malaria vaccine candidate, shows polymorphism between different isolates. Mice were immunized with p190-3, a recombinant protein which contains mostly conserved sequences derived from the p190 protein of the ...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1993.tb00592.x
更新日期:1993-03-01 00:00:00
abstract::Low- and high-dose infections with the murine large intestinal nematode Trichuris muris are associated with induction of adaptive Th1 and Th2 responses, respectively, in mesenteric lymph nodes (MLN). Classical dendritic cells (cDC) accumulate in the large intestinal mucosa and MLN upon T. muris infection, yet their ro...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/pim.12458
更新日期:2017-10-01 00:00:00
abstract::Fasciola hepatica infection has been shown to suppress sheep lymphocyte proliferation in vitro and this is at least partially attributable to excretory/secretory products (ES) released by F. hepatica parasites. We identified a suppressive component in ES by analysing the effect of ES fractions, separated by gel filtra...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1046/j.0141-9838.2001.00438.x
更新日期:2002-02-01 00:00:00
abstract::The role of the spleen in amoebic infection was examined in mice, using strains selected as being either genetically-susceptible (C57BL/6) or genetically-resistant (A/J) to amoebiasis. Splenectomized and sham-operated animals were inoculated intracaecally with 2.5 X 10(5) polyxenic trophozoites of E. histolytica at 6,...
journal_title:Parasite immunology
pub_type: 杂志文章
doi:10.1111/j.1365-3024.1985.tb00093.x
更新日期:1985-09-01 00:00:00