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Dynamic polarization of the microtubule cytoskeleton during CTL-mediated killing.

Abstract：

:Efficient unidirectional killing by cytotoxic T lymphocytes (CTL) requires translocation of the microtubule organizing center (MTOC) to the target cell contact site. Here we utilize modulated polarization microscopy and computerized 3D reconstruction of tubulin and LFA-1 immunofluorescence images to investigate how this is accomplished. The results show that the MTOC is drawn vectorially to the contact site by a microtubule sliding mechanism. Once the MTOC arrives at the contact site, it oscillates laterally. Microtubules loop through and anchor to a ring-shaped zone (pSMAC) defined by the dense clustering of LFA-1 at the target contact site. Microtubules that run straight between the MTOC and pSMAC and then turn sharply may indicate the action of a microtubule motor such as dynein.

journal_name

Immunity

journal_title

Immunity

authors

Kuhn JR,Poenie M

doi

10.1016/s1074-7613(02)00262-5

keywords:

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

111-21

issue

1

eissn

1074-7613

issn

1097-4180

pii

S1074-7613(02)00262-5

journal_volume

16

pub_type

杂志文章

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