Components of a Stat recognition code: evidence for two layers of molecular selectivity.

Abstract:

:Latent and activated forms of Stat1 and Stat6 have been expressed and purified, enabling biochemical experiments relating to their functional activities. Stat1 bound to a phosphotyrosine peptide derived from the IFN gamma receptor with a KD of 50 nM, whereas Stat6 bound to an IL-4 receptor peptide with a KD of 300 nM. Stat-receptor peptide interactions were specific and dependent upon tyrosine phosphorylation. Activated forms of Stat1 and Stat6 were used to select their optimal DNA binding sites. Stat1 selected a recognition site having dyad half-sites separated by 3 bp. Stat6 selected a recognition site composed of the same dyad half-sites, yet separated by 4 bp. Chimeric Stat1-Stat6 recombinants were expressed, purified, and assayed for receptor coupling and DNA binding specificity. Such studies led to the identification of polypeptide domains that specify these activities. These observations provide a framework for understanding how different cytokines elicit distinctive patterns of gene expression.

journal_name

Immunity

journal_title

Immunity

authors

Schindler U,Wu P,Rothe M,Brasseur M,McKnight SL

doi

10.1016/1074-7613(95)90013-6

subject

Has Abstract

pub_date

1995-06-01 00:00:00

pages

689-97

issue

6

eissn

1074-7613

issn

1097-4180

pii

1074-7613(95)90013-6

journal_volume

2

pub_type

杂志文章

相关文献

IMMUNITY文献大全
  • Feeling a little SYK after mixing BAFF with BCR.

    abstract::In this issue of Immunity, Schweighoffer et al. (2013) report that full BAFF responsiveness in B cells requires the tyrosine kinase Syk and that BAFF may co-opt components of the B cell receptor in transmitting its survival signal. ...

    journal_title:Immunity

    pub_type: 评论,杂志文章

    doi:10.1016/j.immuni.2013.03.001

    authors: Infantino S,Tarlinton DM

    更新日期:2013-03-21 00:00:00

  • An interleukin-2 signal relieves BSAP (Pax5)-mediated repression of the immunoglobulin J chain gene.

    abstract::Cytokine regulation of B cell development was analyzed using interleukin-2 (IL-2)-induced transcription of the J chain gene as a model system. A nuclear target of the IL-2 signal was identified as the Pax5 transcription factor, BSAP, which recognizes a negative regulatory motif in the J chain promoter. Functional assa...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/s1074-7613(00)80263-0

    authors: Rinkenberger JL,Wallin JJ,Johnson KW,Koshland ME

    更新日期:1996-10-01 00:00:00

  • Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals.

    abstract::The World Health Organization has declared SARS-CoV-2 virus outbreak a worldwide pandemic. However, there is very limited understanding on the immune responses, especially adaptive immune responses to SARS-CoV-2 infection. Here, we collected blood from COVID-19 patients who have recently become virus-free, and therefo...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2020.04.023

    authors: Ni L,Ye F,Cheng ML,Feng Y,Deng YQ,Zhao H,Wei P,Ge J,Gou M,Li X,Sun L,Cao T,Wang P,Zhou C,Zhang R,Liang P,Guo H,Wang X,Qin CF,Chen F,Dong C

    更新日期:2020-06-16 00:00:00

  • Regulatory T cells sequentially migrate from inflamed tissues to draining lymph nodes to suppress the alloimmune response.

    abstract::To determine the site and mechanism of suppression by regulatory T (Treg) cells, we investigated their migration and function in an islet allograft model. Treg cells first migrated from blood to the inflamed allograft where they were essential for the suppression of alloimmunity. This process was dependent on the chem...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2008.12.022

    authors: Zhang N,Schröppel B,Lal G,Jakubzick C,Mao X,Chen D,Yin N,Jessberger R,Ochando JC,Ding Y,Bromberg JS

    更新日期:2009-03-20 00:00:00

  • Follicular Dendritic Cell Activation by TLR Ligands Promotes Autoreactive B Cell Responses.

    abstract::A hallmark of autoimmunity in murine models of lupus is the formation of germinal centers (GCs) in lymphoid tissues where self-reactive B cells expand and differentiate. In the host response to foreign antigens, follicular dendritic cells (FDCs) maintain GCs through the uptake and cycling of complement-opsonized immun...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2016.12.014

    authors: Das A,Heesters BA,Bialas A,O'Flynn J,Rifkin IR,Ochando J,Mittereder N,Carlesso G,Herbst R,Carroll MC

    更新日期:2017-01-17 00:00:00

  • The distinct roles of TRAF2 and RIP in IKK activation by TNF-R1: TRAF2 recruits IKK to TNF-R1 while RIP mediates IKK activation.

    abstract::The death domain kinase RIP and the TNF receptor-associated factor 2 (TRAF2) are essential effectors in TNF signaling. To understand the mechanism by which RIP and TRAF2 regulate TNF-induced activation of the transcription factor NF-kappaB, we investigated their respective roles in TNF-R1-mediated IKK activation using...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/s1074-7613(00)80194-6

    authors: Devin A,Cook A,Lin Y,Rodriguez Y,Kelliher M,Liu Z

    更新日期:2000-04-01 00:00:00

  • Dynamic polarization of the microtubule cytoskeleton during CTL-mediated killing.

    abstract::Efficient unidirectional killing by cytotoxic T lymphocytes (CTL) requires translocation of the microtubule organizing center (MTOC) to the target cell contact site. Here we utilize modulated polarization microscopy and computerized 3D reconstruction of tubulin and LFA-1 immunofluorescence images to investigate how th...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/s1074-7613(02)00262-5

    authors: Kuhn JR,Poenie M

    更新日期:2002-01-01 00:00:00

  • In vivo identification of novel regulators and conserved pathways of phagocytosis in A. gambiae.

    abstract::Anopheles gambiae uses effective immune responses, including phagocytosis, to fight microbial infection. We have developed a semiquantitative phagocytosis test and used it in conjunction with dsRNA gene silencing to test the in vivo roles of 71 candidate genes in phagocytosis of Escherichia coli and Staphylococcus aur...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2005.05.006

    authors: Moita LF,Wang-Sattler R,Michel K,Zimmermann T,Blandin S,Levashina EA,Kafatos FC

    更新日期:2005-07-01 00:00:00

  • Cell-Type-Specific Interleukin 1 Receptor 1 Signaling in the Brain Regulates Distinct Neuroimmune Activities.

    abstract::Interleukin-1 (IL-1) signaling is important for multiple potentially pathogenic processes in the central nervous system (CNS), but the cell-type-specific roles of IL-1 signaling are unclear. We used a genetic knockin reporter system in mice to track and reciprocally delete or express IL-1 receptor 1 (IL-1R1) in specif...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2018.12.012

    authors: Liu X,Nemeth DP,McKim DB,Zhu L,DiSabato DJ,Berdysz O,Gorantla G,Oliver B,Witcher KG,Wang Y,Negray CE,Vegesna RS,Sheridan JF,Godbout JP,Robson MJ,Blakely RD,Popovich PG,Bilbo SD,Quan N

    更新日期:2019-02-19 00:00:00

  • A Little Fiber Goes a Long Way.

    abstract::In this issue of Immunity, Trompette et al. (2018) show that dietary fiber and short chain fatty acids reduce influenza A virus-associated immunopathology by enhancing CD8+ T cell effector function and by promoting the differentiation of alternatively activated macrophages. ...

    journal_title:Immunity

    pub_type: 评论,杂志文章

    doi:10.1016/j.immuni.2018.05.001

    authors: Castillo PAC,Hand TW

    更新日期:2018-05-15 00:00:00

  • Immune-cell lineage commitment: translation from mice to humans.

    abstract::Much of the current understanding in hematopoietic stem cell differentiation into immune-cell lineages comes from mouse studies, but how well does it translate to the human system? ...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2007.05.011

    authors: Payne KJ,Crooks GM

    更新日期:2007-06-01 00:00:00

  • T cell receptor delta gene rearrangement and T early alpha (TEA) expression in immature alpha beta lineage thymocytes: implications for alpha beta/gamma delta lineage commitment.

    abstract::Mature T cells comprise two mutually exclusive lineages expressing heterodimeric alpha beta or gamma delta antigen receptors. During development, beta, gamma, and delta genes rearrange before alpha, and mature gamma delta cells arise in the thymus prior to alpha beta cells. The mechanism underlying commitment of immat...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/s1074-7613(00)80296-4

    authors: Wilson A,de Villartay JP,MacDonald HR

    更新日期:1996-01-01 00:00:00

  • The HVEM-BTLA Axis Restrains T Cell Help to Germinal Center B Cells and Functions as a Cell-Extrinsic Suppressor in Lymphomagenesis.

    abstract::The tumor necrosis factor receptor superfamily member HVEM is one of the most frequently mutated surface proteins in germinal center (GC)-derived B cell lymphomas. We found that HVEM deficiency increased B cell competitiveness during pre-GC and GC responses. The immunoglobulin (Ig) superfamily protein BTLA regulated H...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2019.05.022

    authors: Mintz MA,Felce JH,Chou MY,Mayya V,Xu Y,Shui JW,An J,Li Z,Marson A,Okada T,Ware CF,Kronenberg M,Dustin ML,Cyster JG

    更新日期:2019-08-20 00:00:00

  • Plague's partners in crime.

    abstract::The hallmark of bubonic plague is the presence of grotesquely swollen lymph nodes, called buboes. This frenzied inflammatory response to Yersinia pestis is poorly understood. In this issue of Immunity, St. John et al. (2014) explore the mechanism by which Y. pestis spreads and thus leads to this striking lymphadenopat...

    journal_title:Immunity

    pub_type: 评论,杂志文章

    doi:10.1016/j.immuni.2014.09.003

    authors: Davis KM,Isberg RR

    更新日期:2014-09-18 00:00:00

  • Alternative activation of macrophages: mechanism and functions.

    abstract::The concept of an alternative pathway of macrophage activation has stimulated interest in its definition, mechanism, and functional significance in homeostasis and disease. We assess recent research in this field, argue for a restricted definition, and explore pathways by which the T helper 2 (Th2) cell cytokines inte...

    journal_title:Immunity

    pub_type: 杂志文章,评审

    doi:10.1016/j.immuni.2010.05.007

    authors: Gordon S,Martinez FO

    更新日期:2010-05-28 00:00:00

  • Clec2d Joins the Cell Death Sensor Ranks.

    abstract::Sensing tissue damage is an ancient function of immune cells that is central to the regulation of inflammation, tissue repair, and immunity. In this issue of Immunity, Lai et al. (2020) uncover a role for the C-type lectin receptor Clec2d as a sensor of cell death, which directly detects histones released during necro...

    journal_title:Immunity

    pub_type: 评论,杂志文章

    doi:10.1016/j.immuni.2019.12.015

    authors: Del Fresno C,Sancho D

    更新日期:2020-01-14 00:00:00

  • A single subset of dendritic cells controls the cytokine bias of natural killer T cell responses to diverse glycolipid antigens.

    abstract::Many hematopoietic cell types express CD1d and are capable of presenting glycolipid antigens to invariant natural killer T cells (iNKT cells). However, the question of which cells are the principal presenters of glycolipid antigens in vivo remains controversial, and it has been suggested that this might vary depending...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2013.12.004

    authors: Arora P,Baena A,Yu KO,Saini NK,Kharkwal SS,Goldberg MF,Kunnath-Velayudhan S,Carreño LJ,Venkataswamy MM,Kim J,Lazar-Molnar E,Lauvau G,Chang YT,Liu Z,Bittman R,Al-Shamkhani A,Cox LR,Jervis PJ,Veerapen N,Besra GS,Por

    更新日期:2014-01-16 00:00:00

  • Group 3 innate lymphoid cells inhibit T-cell-mediated intestinal inflammation through aryl hydrocarbon receptor signaling and regulation of microflora.

    abstract::Aryl hydrocarbon receptor (Ahr) is crucial for the maintenance and function of group 3 innate lymphoid cells (ILCs), which are important in gut immunity. Because Ahr promotes T helper 17 (Th17) cell differentiation in vitro, it is reasonable to expect that Ahr would enhance Th17 cells in vivo. Instead, we show that Ah...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2013.08.002

    authors: Qiu J,Guo X,Chen ZM,He L,Sonnenberg GF,Artis D,Fu YX,Zhou L

    更新日期:2013-08-22 00:00:00

  • Monocyte subsets responsible for immunoglobulin G-dependent effector functions in vivo.

    abstract::Immunoglobulin G (IgG) antibodies confer protection against pathogenic microorganisms, serve as therapeutics in tumor therapy, and are involved in destruction of healthy tissues during autoimmune diseases. Understanding the molecular pathways and effector cell types involved in antibody-mediated effector functions is ...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2011.11.009

    authors: Biburger M,Aschermann S,Schwab I,Lux A,Albert H,Danzer H,Woigk M,Dudziak D,Nimmerjahn F

    更新日期:2011-12-23 00:00:00

  • The Miami Monkey: A Sunny Alternative to the Berlin Patient.

    abstract::Curing HIV infection has been impossible, with the exception of the "Berlin Patient." Martinez-Navio et al. (2019) in Miami herein present a rare monkey whose virus was controlled for >3 years after a single genetic intervention that led to persistent production of HIV-neutralizing antibodies in vivo. ...

    journal_title:Immunity

    pub_type: 评论,杂志文章

    doi:10.1016/j.immuni.2019.02.010

    authors: Liberatore RA,Ho DD

    更新日期:2019-03-19 00:00:00

  • Processing of some antigens by the standard proteasome but not by the immunoproteasome results in poor presentation by dendritic cells.

    abstract::By stimulating human lymphocytes with an autologous renal carcinoma, we obtained CTL recognizing an antigen derived from a novel, ubiquitous protein. The CTL failed to lyse autologous EBV-transformed B cells, even though the latter express the protein. This is due to the presence in these cells of immunoproteasomes, w...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/s1074-7613(00)80163-6

    authors: Morel S,Lévy F,Burlet-Schiltz O,Brasseur F,Probst-Kepper M,Peitrequin AL,Monsarrat B,Van Velthoven R,Cerottini JC,Boon T,Gairin JE,Van den Eynde BJ

    更新日期:2000-01-01 00:00:00

  • Memory B cells from human tonsils colonize mucosal epithelium and directly present antigen to T cells by rapid up-regulation of B7-1 and B7-2.

    abstract::Human memory B cells that carry mutated IgV region genes were isolated from tonsils by negative selection of IgD+ naive B cells and CD38+ germinal center B cells and plasma cells. They were mainly found within the intraepithelial areas, but not in the B cell follicles of human tonsils. Memory B cells but not naive B c...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/1074-7613(95)90048-9

    authors: Liu YJ,Barthélémy C,de Bouteiller O,Arpin C,Durand I,Banchereau J

    更新日期:1995-03-01 00:00:00

  • The costimulatory molecule CD27 maintains clonally diverse CD8(+) T cell responses of low antigen affinity to protect against viral variants.

    abstract::CD70 and CD27 are costimulatory molecules that provide essential signals for the expansion and differentiation of CD8(+) T cells. Here, we show that CD27-driven costimulation lowered the threshold of T cell receptor activation on CD8(+) T cells and enabled responses against low-affinity antigens. Using influenza infec...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2011.04.020

    authors: van Gisbergen KP,Klarenbeek PL,Kragten NA,Unger PP,Nieuwenhuis MB,Wensveen FM,ten Brinke A,Tak PP,Eldering E,Nolte MA,van Lier RA

    更新日期:2011-07-22 00:00:00

  • Epstein-Barr virus coopts lipid rafts to block the signaling and antigen transport functions of the BCR.

    abstract::The B cell antigen receptor (BCR) functions to initiate signaling and to internalize antigen for processing from within Lyn kinase-enriched membrane lipid rafts. The signaling function of the BCR is blocked by Epstein-Barr Virus (EBV) latent membrane protein 2A (LMP2A), which is constitutively phosphorylated by Lyn. H...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/s1074-7613(01)00089-9

    authors: Dykstra ML,Longnecker R,Pierce SK

    更新日期:2001-01-01 00:00:00

  • Complement is a central mediator of radiotherapy-induced tumor-specific immunity and clinical response.

    abstract::Radiotherapy induces DNA damage and cell death, but recent data suggest that concomitant immune stimulation is an integral part of the therapeutic action of ionizing radiation. It is poorly understood how radiotherapy supports tumor-specific immunity. Here we report that radiotherapy induced tumor cell death and trans...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2015.03.009

    authors: Surace L,Lysenko V,Fontana AO,Cecconi V,Janssen H,Bicvic A,Okoniewski M,Pruschy M,Dummer R,Neefjes J,Knuth A,Gupta A,van den Broek M

    更新日期:2015-04-21 00:00:00

  • TIM-1 glycoprotein binds the adhesion receptor P-selectin and mediates T cell trafficking during inflammation and autoimmunity.

    abstract::Selectins play a central role in leukocyte trafficking by mediating tethering and rolling on vascular surfaces. Here we have reported that T cell immunoglobulin and mucin domain 1 (TIM-1) is a P-selectin ligand. We have shown that human and murine TIM-1 binds to P-selectin, and that TIM-1 mediates tethering and rollin...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2014.03.004

    authors: Angiari S,Donnarumma T,Rossi B,Dusi S,Pietronigro E,Zenaro E,Della Bianca V,Toffali L,Piacentino G,Budui S,Rennert P,Xiao S,Laudanna C,Casasnovas JM,Kuchroo VK,Constantin G

    更新日期:2014-04-17 00:00:00

  • Regulatory T cell lineage specification by the forkhead transcription factor foxp3.

    abstract::Regulatory T cell-mediated dominant tolerance has been demonstrated to play an important role in the prevention of autoimmunity. Here, we present data arguing that the forkhead transcription factor Foxp3 acts as the regulatory T cell lineage specification factor and mediator of the genetic mechanism of dominant tolera...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2005.01.016

    authors: Fontenot JD,Rasmussen JP,Williams LM,Dooley JL,Farr AG,Rudensky AY

    更新日期:2005-03-01 00:00:00

  • Pulmonary Epithelial Cell-Derived Cytokine TGF-β1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.

    abstract::Epithelial cells orchestrate pulmonary homeostasis and pathogen defense and play a crucial role in the initiation of allergic immune responses. Maintaining the balance between homeostasis and inappropriate immune activation and associated pathology is particularly complex at mucosal sites that are exposed to billions ...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2015.10.012

    authors: Denney L,Byrne AJ,Shea TJ,Buckley JS,Pease JE,Herledan GM,Walker SA,Gregory LG,Lloyd CM

    更新日期:2015-11-17 00:00:00

  • A range of CD4 T cell tolerance: partial inactivation to organ-specific antigen allows nondestructive thyroiditis or insulitis.

    abstract::T cell receptor (TCR) transgenic mice specific for hen egg lysozyme (HEL) were crossed with mice expressing HEL on the thyroid epithelium, on pancreatic islet beta cells, or systemically. Depending on the pattern of HEL expression, deletion of double-positive thymocytes ranged from minimal to complete, and peripheral ...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/s1074-7613(00)80528-2

    authors: Akkaraju S,Ho WY,Leong D,Canaan K,Davis MM,Goodnow CC

    更新日期:1997-08-01 00:00:00

  • The transcription factor GATA3 is essential for the function of human type 2 innate lymphoid cells.

    abstract::Type 2 innate lymphoid cells (ILC2s) are part of a large family of ILCs that are important effectors in innate immunity, lymphoid organogenesis, and tissue remodeling. ILC2s mediate parasite expulsion but also contribute to airway inflammation, emphasizing the functional similarity between these cells and Th2 cells. C...

    journal_title:Immunity

    pub_type: 杂志文章

    doi:10.1016/j.immuni.2012.08.015

    authors: Mjösberg J,Bernink J,Golebski K,Karrich JJ,Peters CP,Blom B,te Velde AA,Fokkens WJ,van Drunen CM,Spits H

    更新日期:2012-10-19 00:00:00