Abstract：
:The non-receptor protein tyrosine kinase Fyn, which is a member of the Src family of kinases, has been shown to be essential for normal myelination and has been suggested to play a role in oligodendrocyte development. However, oligodendrocyte development has not been studied directly in cells lacking Fyn. Additionally, because Fyn is expressed in neurons as well as oligodendrocytes, it is possible that normal myelination requires Fyn expression in neurons but not in oligodendrocytes. To address these issues, we analyzed the development of oligodendrocytes in neuron-free glial cell cultures from fyn(-/-) mice that express no Fyn protein. We observed that oligodendrocytes develop to the stage where they elaborate an extensive network of membranous processes and express the antigenic components of mature oligodendrocytes in the complete absence of Fyn. However, as compared with fyn(+/+) controls, fewer oligodendroglia developed in fyn(-/-) cell cultures, and a smaller proportion of them matured to the stage characterized by a high degree of morphological complexity. In addition, we found that insulin-like growth factor-I, a potent stimulator of oligodendrocyte development, failed to stimulate morphological maturation of fyn(-/-) oligodendroglia. The pyrazolopyrimidine PP2, believed to be a selective inhibitor of Fyn, did not prevent the development of morphologically complex oligodendrocytes. Unexpectedly, however, it was toxic to both fyn(+/+) and fyn(-/-) glial cells, indicating that this class of inhibitors can have significant effects that are independent of Fyn.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Sperber BR,McMorris FAdoi
10.1002/1097-4547(20010215)63:4<303::AID-JNR1024>3keywords:
subject
Has Abstractpub_date
2001-02-15 00:00:00pages
303-12issue
4eissn
0360-4012issn
1097-4547pii
10.1002/1097-4547(20010215)63:4<303::AID-JNR1024>3journal_volume
63pub_type
杂志文章abstract：:A-type K+ channels (IA channels) contribute to learning and memory mechanisms by regulating neuronal excitabilities in the CNS, and their expression level is targeted by Ca2+ influx via synaptic NMDA receptors (NMDARs) during long-term potentiation (LTP). However, it is not clear how local synaptic Ca2+ changes induce...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.24076
更新日期：2017-12-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章,评审
doi：
更新日期：2000-02-01 00:00:00
abstract：:Organotypic slices are used frequently in studies of central nervous system development and function because they provide excellent experimental access with significant preservation of cellular context and relationships. Within a slice, however, a variety of factors may cause individual classes of neurons to respond d...
journal_title：Journal of neuroscience research
pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2018-07-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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更新日期：1990-06-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20105
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pub_type： 杂志文章
doi：10.1002/jnr.490080407
更新日期：1982-01-01 00:00:00
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pub_type： 杂志文章
doi：10.1002/jnr.490170108
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pub_type： 杂志文章,评审
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22563
更新日期：2011-03-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20131
更新日期：2004-06-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490370512
更新日期：1994-04-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22132
更新日期：2009-10-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.22037
更新日期：2009-07-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490370117
更新日期：1994-01-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/(SICI)1097-4547(19980615)52:6<709::AID-JNR
更新日期：1998-06-15 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1997-07-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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更新日期：2011-02-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490100407
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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更新日期：2003-12-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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更新日期：1983-01-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.490270318
更新日期：1990-11-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
doi：10.1002/jnr.20166
更新日期：2004-08-01 00:00:00
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journal_title：Journal of neuroscience research
pub_type： 杂志文章
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更新日期：2001-11-15 00:00:00
