Abstract：
:Biomass and secondary metabolite accumulation were assayed on a wide range of auxins, cytokinins and their combinations using Ecballium elaterium callus tissue. The best combination was that consisting of alpha-naphthalene acetic acid (NAA) and benzylamino purine (BAP) (1 mg/l of medium, each). NAA and BAP were set up in a grid to determine concentrations that produced the best callus as regards fresh and dry weights and yield of cucurbitacins, particularly cucurbitacin E. The best combination for callus proliferation consisted of 5 mg/l NAA and 5 mg/l BAP, reaching a peak at week 5. For cucurbitacin production, an optimum yield was obtained at week 3 with 5 mg/l NAA.
journal_name
Fitoterapiajournal_title
Fitoterapiaauthors
Attard EG,Scicluna-Spiteri Adoi
10.1016/s0367-326x(00)00256-2keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
46-53issue
1eissn
0367-326Xissn
1873-6971pii
S0367326X00002562journal_volume
72pub_type
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