Abstract:
:Chromosomal assignment of human transcribed sequences has been done mainly by high throughput genome analysis in specialized genome centres and, in a more classical fashion, by fluorescence in-situ hybridization (FISH) analysis. Not every laboratory has the ability to map cDNAs by FISH analysis. We here report a rapid mapping approach that is based on the hybridization of cDNA probes to high density gridded CEPH-YAC filters followed by subsequent computational analysis by database searches in the internet. Not only transcribed sequences but also genomic DNA could be subjected to this mapping approach. The presented approach allows to map human transcribed and genomic DNAs within 1-3 days and with a high level of resolution that will constantly increase in line with the incorporation of data deriving from high throughput genome mapping.
journal_name
Cancer Lettjournal_title
Cancer lettersauthors
Claas A,Savelyeva L,Pillmann A,Schwab Mdoi
10.1016/s0304-3835(00)00690-xkeywords:
subject
Has Abstractpub_date
2001-01-10 00:00:00pages
125-31issue
1eissn
0304-3835issn
1872-7980pii
S0304-3835(00)00690-Xjournal_volume
162pub_type
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