Abstract:
:Human inducible pluripotent stem cells (hiPSCs) hold a large potential for disease modeling. hiPSC-derived human astrocyte and neuronal cultures permit investigations of neural signaling pathways with subcellular resolution. Combinatorial cultures, and three-dimensional (3-D) embryonic bodies (EBs) enlarge the scope of investigations to multi-cellular phenomena. The highest level of complexity, brain organoids that-in many aspects-recapitulate anatomical and functional features of the developing brain permit the study of developmental and morphological aspects of human disease. An ideal microscope for 3-D tissue imaging at these different scales would combine features from both confocal laser-scanning and light-sheet microscopes: a micrometric optical sectioning capacity and sub-micrometric spatial resolution, a large field of view and high frame rate, and a low degree of invasiveness, i.e., ideally, a better photon efficiency than that of a confocal microscope. In the present work, we describe such an instrument that uses planar two-photon (2P) excitation. Its particularity is that-unlike two- or three-lens light-sheet microscopes-it uses a single, low-magnification, high-numerical aperture objective for the generation and scanning of a virtual light sheet. The microscope builds on a modified Nipkow-Petráň spinning-disk scheme for achieving wide-field excitation. However, unlike the Yokogawa design that uses a tandem disk, our concept combines micro lenses, dichroic mirrors and detection pinholes on a single disk. This new design, advantageous for 2P excitation, circumvents problems arising with the tandem disk from the large wavelength difference between the infrared excitation light and visible fluorescence. 2P fluorescence excited by the light sheet is collected with the same objective and imaged onto a fast sCMOS camera. We demonstrate 3-D imaging of TO-PRO3-stained EBs and of brain organoids, uncleared and after rapid partial transparisation with triethanolamine formamide (RTF) and we compare the performance of our instrument to that of a confocal laser-scanning microscope (CLSM) having a similar numerical aperture. Our large-field 2P-spinning disk microscope permits one order of magnitude faster imaging, affords less photobleaching and permits better depth penetration than a confocal microscope with similar spatial resolution.
journal_name
Front Neuroanatjournal_title
Frontiers in neuroanatomyauthors
Rakotoson I,Delhomme B,Djian P,Deeg A,Brunstein M,Seebacher C,Uhl R,Ricard C,Oheim Mdoi
10.3389/fnana.2019.00077subject
Has Abstractpub_date
2019-08-20 00:00:00pages
77issn
1662-5129journal_volume
13pub_type
杂志文章abstract::Oxytocin, a neuropeptide and peptide hormone, is produced by neurons in the hypothalamus and released by the posterior pituitary to control breastfeeding and labor. Recent studies have revealed that oxytocin in the central nervous system is also involved in modulating social interaction. To understand the potential ro...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2020.559402
更新日期:2020-10-19 00:00:00
abstract::The development of the mammalian neocortex relies heavily on subplate. The proportion of this cell population varies considerably in different mammalian species. Subplate is almost undetectable in marsupials, forms a thin, but distinct layer in mouse and rat, a larger layer in carnivores and big-brained mammals as pig...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2011.00025
更新日期:2011-04-07 00:00:00
abstract::A60, the mouse monoclonal antibody against the neuronal nuclear protein (NeuN), is the most widely used neuronal marker in neuroscience research and neuropathological assays. Previous studies identified fragments of A60-immunoprecipitated protein as Synapsin I (Syn I), suggesting the antibody will demonstrate cross im...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2016.00054
更新日期:2016-05-13 00:00:00
abstract::Calcium stores in neurons are heterogeneous in compartmentalization and molecular composition. Danio rerio (zebrafish) is an animal model with a simply folded cerebellum similar in cellular organization to that of mammals. The aim of the study was to identify new endoplasmic reticulum (ER) calcium store markers in zeb...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2020.00015
更新日期:2020-04-21 00:00:00
abstract::Traditionally, the impact of evolution on the central nervous system has been studied by comparing the sizes of brain regions between species. However, more recent work has demonstrated that environmental factors, such as sensory experience, modulate brain region sizes intraspecifically, clouding the distinction betwe...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2020.00010
更新日期:2020-03-18 00:00:00
abstract::Larger mammalian cerebral cortices tend to have increasingly folded surfaces, often considered to result from the lateral expansion of the gray matter (GM), which, in a volume constrained by the cranium, causes mechanical compression that is relieved by inward folding of the white matter (WM), or to result from differ...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2012.00003
更新日期:2012-02-02 00:00:00
abstract::Enough species have now been subject to systematic quantitative analysis of the relationship between the morphology and cellular composition of their brain that patterns begin to emerge and shed light on the evolutionary path that led to mammalian brain diversity. Based on an analysis of the shared and clade-specific ...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章,评审
doi:10.3389/fnana.2014.00077
更新日期:2014-08-11 00:00:00
abstract::Polysialic acid (polySia) is a complex sugar that in the nervous system appears mainly as a posttranslational modification of the neural cell adhesion molecule (NCAM). PolySia plays important roles during brain development, but also in its plasticity during adulthood. Two polysialyltransferases (polyST), ST8SIA2 and S...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2019.00006
更新日期:2019-02-06 00:00:00
abstract::Dopamine (DA) modulates the effects of amino acid neurotransmitters (AANs), including GABA and glutamate, in motor, visual, olfactory, and reward systems (Hnasko et al., 2010; Stuber et al., 2010; Hnasko and Edwards, 2012). The results suggest that DA may play a similar modulatory role in the auditory pathways. Previo...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2015.00088
更新日期:2015-07-22 00:00:00
abstract::Ketamine, a known antagonist of N-methyl-D-aspartic (NMDA) glutamate receptors, had been used as an anesthetic particularly for pediatric or for cardiac patients. Unfortunately, ketamine has become an abusive drug in many parts of the world while chronic and prolonged usage led to damages of many organs including the ...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2013.00023
更新日期:2013-07-17 00:00:00
abstract::Although the olfactory sense has always been considered with less interest than the visual, auditive or somatic senses, it does plays a major role in our ordinary life, with important implication in dangerous situations or in social and emotional behaviors. Traditional Diffusion Tensor signal model and related tractog...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2017.00032
更新日期:2017-04-10 00:00:00
abstract::Expression patterns of Nkx2.1 and Islet-1 (Isl1), which encode transcription factors that are key in the regionalization of the forebrain, were analyzed by combined immunohistochemical methods in young adult specimens of two lungfishes (Neoceratodus forsteri and Protopterus dolloi) and a urodele amphibian (Pleurodeles...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2018.00042
更新日期:2018-05-18 00:00:00
abstract::Cortical networks are shaped by sensory experience and are most susceptible to modifications during critical periods characterized by enhanced plasticity at the structural and functional level. A system particularly well-studied in this context is the mammalian visual system. Plasticity has been documented for the som...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2014.00011
更新日期:2014-03-11 00:00:00
abstract::Rafael Lorente de Nó, the youngest of Santiago Ramón y Cajal disciples, was one of the last Century's more influential researches in neuroscience. This assay highlights two fundamental contributions of Rafael Lorente de Nó to neurobiology: the intrinsic organization of the mammalian cerebral cortex and the basic physi...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章,评审
doi:10.3389/fnana.2014.00147
更新日期:2014-12-03 00:00:00
abstract::Involuntary movements or dyskinesia, represent a debilitating complication of levodopa therapy for Parkinson's disease. Dyskinesia is, ultimately, experienced by the vast majority of the patients. Despite the importance of this problem, little was known about the cause of dyskinesia, a situation that has dramatically ...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2010.00131
更新日期:2010-09-14 00:00:00
abstract::How does the size of the glial and neuronal cells that compose brain tissue vary across brain structures and species? Our previous studies indicate that average neuronal size is highly variable, while average glial cell size is more constant. Measuring whole cell sizes in vivo, however, is a daunting task. Here we use...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2014.00127
更新日期:2014-11-12 00:00:00
abstract::Expansion microscopy and light sheet imaging (ExLSM) provide a viable alternative to existing tissue clearing and large volume imaging approaches. The analysis of intact volumes of brain tissue presents a distinct challenge in neuroscience. Recent advances in tissue clearing and light sheet microscopy have re-addresse...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2019.00002
更新日期:2019-01-31 00:00:00
abstract::Species-typical patterns of grouping have profound impacts on many aspects of physiology and behavior. However, prior to our recent studies in estrildid finches, neural mechanisms that titrate species-typical group-size preferences, independent of other aspects of social organization (e.g., mating system and parental ...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2011.00013
更新日期:2011-03-02 00:00:00
abstract::Sevoflurane is widely used in adult and pediatric patients during clinical surgeries. Although studies have shown that exposure to sevoflurane impairs solfactory memory after an operation, the neuropathological changes underlying this effect are not clear. This study detected the effect of sevoflurane exposure on the ...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2016.00072
更新日期:2016-06-24 00:00:00
abstract::In mammalian cerebrum there exist two distinct types of interneurons expressing nitric oxide synthase (NOS). Type I neurons are large in size and exhibit heavy nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) histochemical reaction, while type II cells are small with light NADPH-d reactivity. The time ...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2015.00011
更新日期:2015-02-16 00:00:00
abstract::The claustrum is a narrow subcortical brain structure that resides between the striatum and insular cortex. The function of the claustrum is not fully described, and while our previous work supports a role for the claustrum in top-down cognitive control of action, other evidence suggests the claustrum may be involved ...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2018.00100
更新日期:2018-11-26 00:00:00
abstract::The white matter of the central nervous system (CNS) is difficult to represent in anatomy because it is located predominantly "between" other anatomical entities. In a classic presentation, like a cross section of a brain segment, white matter is present and can be labeled adequately. Several appearances of the same e...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2018.00102
更新日期:2018-12-13 00:00:00
abstract::Early telencephalic development involves the migration of diverse cell types that can be identified by specific molecular markers. Most prominent among them are Cajal-Retzius (CR) cells that emanate mainly from the cortical hem and to a lesser extent from rostrolateral, septal and caudo-medial regions. One additional ...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2017.00097
更新日期:2017-11-01 00:00:00
abstract::Adult born neurons in the hippocampus show species-specific differences in their numbers, the pace of their maturation and their spatial distribution. Here, we present quantitative data on adult hippocampal neurogenesis in a New World primate, the common marmoset (Callithrix jacchus) that demonstrate parts of the line...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2015.00085
更新日期:2015-06-29 00:00:00
abstract::Imaging entire mouse brains at submicron resolution has historically been a challenging undertaking and largely confined to the province of dedicated atlasing initiatives. This has limited systematic investigations into important areas of neuroscience, such as neural circuits, brain mapping and neurodegeneration. In t...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2016.00031
更新日期:2016-03-22 00:00:00
abstract::Why do neurons have dendritic spines? This question-the heart of what Yuste calls "the spine problem"-presupposes that why-questions of this sort have scientific answers: that empirical findings can favor or count against claims about why neurons have spines. Here we show how such questions can receive empirical answe...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2014.00095
更新日期:2014-09-10 00:00:00
abstract::In the olfactory bulb (OB) a sophisticated neuronal network mediates the primary processing of sensory information and extensive investigations over the past decades have greatly improved our understanding of the morphology and neuronal organization of the OB. However, efforts have mostly been focused on the different...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2017.00125
更新日期:2017-12-18 00:00:00
abstract::In rodents, sexual behavior depends on the adequate detection of sexually relevant stimuli. The olfactory bulb (OB) is a region of the adult mammalian brain undergoing constant cell renewal by continuous integration of new granular and periglomerular neurons in the accessory (AOB) and main (MOB) olfactory bulbs. The p...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2012.00025
更新日期:2012-07-06 00:00:00
abstract::Drug addiction is a severe neuropsychiatric disorder characterized by loss of control over motivated behavior. The need for effective treatments mandates a greater understanding of the causes and identification of new therapeutic targets for drug development. Drugs of abuse subjugate normal reward-related behavior to ...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2011.00060
更新日期:2011-09-20 00:00:00
abstract::Pathways arising from the ventral tegmental area (VTA) release dopamine and other neurotransmitters during the expectation and achievement of reward, and are regarded as central links of the brain networks that create drive, pleasure, and addiction. While the global pattern of VTA projections is well-known, the actual...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2015.00059
更新日期:2015-05-19 00:00:00