Abstract:
:Imaging entire mouse brains at submicron resolution has historically been a challenging undertaking and largely confined to the province of dedicated atlasing initiatives. This has limited systematic investigations into important areas of neuroscience, such as neural circuits, brain mapping and neurodegeneration. In this article, we describe in detail Serial Two-Photon (STP) tomography, a robust, reliable method for imaging entire brains with histological detail. We provide examples of how the basic methodology can be extended to other imaging modalities, such as Optical Coherence Tomography (OCT), in order to provide unique contrast mechanisms. Furthermore, we provide a survey of the research that STP tomography has enabled in the field of neuroscience, provide examples of how this technology enables quantitative whole brain studies, and discuss the current limitations of STP tomography-based approaches.
journal_name
Front Neuroanatjournal_title
Frontiers in neuroanatomyauthors
Amato SP,Pan F,Schwartz J,Ragan TMdoi
10.3389/fnana.2016.00031subject
Has Abstractpub_date
2016-03-22 00:00:00pages
31issn
1662-5129journal_volume
10pub_type
杂志文章abstract::The white matter (WM) of the adult human neocortex contains the so-called "interstitial neurons". They are most numerous in the superficial WM underlying the cortical gyri, and decrease in density toward the deep WM. They are morphologically heterogeneous. A subgroup of interstitial neurons display pyramidal-cell like...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/neuro.05.007.2009
更新日期:2009-06-09 00:00:00
abstract::Sevoflurane is widely used in adult and pediatric patients during clinical surgeries. Although studies have shown that exposure to sevoflurane impairs solfactory memory after an operation, the neuropathological changes underlying this effect are not clear. This study detected the effect of sevoflurane exposure on the ...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2016.00072
更新日期:2016-06-24 00:00:00
abstract::Dual transneuronal tracing is a novel viral tracing methodology which employs two recombinant viruses, each expressing a different reporter protein. Peripheral injection of recombinant pseudorabies viruses has been used as a powerful method to define neurons that coordinate outputs to various peripheral targets of mot...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/neuro.05.001.2009
更新日期:2009-01-19 00:00:00
abstract::Introduction: Knowledge of the neuro-anatomical architecture of the inner ear contributes to the improvement and development of cochlear and vestibular implants. The present knowledge is mainly based on two-dimensional images (histology) or derived models that simplify the complexity of this architecture. This study i...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2018.00041
更新日期:2018-05-22 00:00:00
abstract::In human prefrontal cortex (PFC), ~85% of γ-aminobutyric acid (GABA)-expressing neurons can be subdivided into non-overlapping groups by the presence of calbindin (CB), calretinin (CR) or parvalbumin (PV). Substantial research has focused on the differences in the laminar locations of the cells bodies of these neurons...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2018.00009
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abstract::Dendrite and dendritic spine formation are crucial for proper brain function. DISC1-binding zinc finger protein (DBZ) was first identified as a Disrupted-In-Schizophrenia1 (DISC1) binding partner. DBZ is highly expressed in the cerebral cortex of developing and adult rodents and is involved in neurite formation, cell ...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2015.00052
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abstract::Non-invasive in vivo neuroimaging techniques provide a wide array of possibilities to study human brain function. A number of approaches are available that improve our understanding of the anatomical location of brain activation patterns, including the development of probabilistic conversion tools to register individu...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2018.00110
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journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2011.00060
更新日期:2011-09-20 00:00:00
abstract::In rodents, sexual behavior depends on the adequate detection of sexually relevant stimuli. The olfactory bulb (OB) is a region of the adult mammalian brain undergoing constant cell renewal by continuous integration of new granular and periglomerular neurons in the accessory (AOB) and main (MOB) olfactory bulbs. The p...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2012.00025
更新日期:2012-07-06 00:00:00
abstract::[This corrects the article on p. 99 in vol. 9, PMID: 26300740.]. ...
journal_title:Frontiers in neuroanatomy
pub_type: 已发布勘误
doi:10.3389/fnana.2015.00146
更新日期:2015-11-17 00:00:00
abstract::Ketamine, a known antagonist of N-methyl-D-aspartic (NMDA) glutamate receptors, had been used as an anesthetic particularly for pediatric or for cardiac patients. Unfortunately, ketamine has become an abusive drug in many parts of the world while chronic and prolonged usage led to damages of many organs including the ...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2013.00023
更新日期:2013-07-17 00:00:00
abstract::Experiments in several species have identified direct projections to the medial geniculate nucleus (MG) from cells in subcollicular auditory nuclei. Moreover, many cochlear nucleus cells that project to the MG send collateral projections to the inferior colliculus (IC) (Schofield et al., 2014). We conducted three expe...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2014.00070
更新日期:2014-07-18 00:00:00
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journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2013.00015
更新日期:2013-06-03 00:00:00
abstract::Embryonic development in mammals has evolved a platform for genomic conflict between mothers and embryos and, by extension, between maternal and paternal genomes. The evolutionary interests of the mother and embryo may be maximized through the promotion of sex-chromosome genes and imprinted alleles, resulting in the r...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2013.00002
更新日期:2013-04-09 00:00:00
abstract::A60, the mouse monoclonal antibody against the neuronal nuclear protein (NeuN), is the most widely used neuronal marker in neuroscience research and neuropathological assays. Previous studies identified fragments of A60-immunoprecipitated protein as Synapsin I (Syn I), suggesting the antibody will demonstrate cross im...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2016.00054
更新日期:2016-05-13 00:00:00
abstract::Immunohistochemistry (IHC) is a valuable method for identifying discrete neurochemical molecules by the interaction of target antigens with validated antibodies tagged with a visible label (e.g., peroxidase). We have developed an immunostaining method that is highly sensitive in detection of neurochemical antigens. Ou...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2015.00022
更新日期:2015-03-03 00:00:00
abstract::The mammalian oculomotor nucleus receives a strong γ-aminobutyric acid (GABA)ergic synaptic input, whereas such projections have rarely been reported in fish. In order to determine whether this synaptic organization is preserved across vertebrates, we investigated the GABAergic projections to the oculomotor nucleus in...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2011.00007
更新日期:2011-02-04 00:00:00
abstract::The auditory system contains a diverse array of interconnected anatomical structures that mediate the perception of sound. The cochlear nucleus of the hindbrain serves as the initial site of convergence for auditory stimuli, while the inferior colliculus of the midbrain serves as an integration and relay station for a...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2017.00067
更新日期:2017-08-22 00:00:00
abstract::Dendritic spines are key components of a variety of microcircuits and they represent the majority of postsynaptic targets of glutamatergic axon terminals in the brain. The present article will focus on the discovery of dendritic spines, which was possible thanks to the application of the Golgi technique to the study o...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章,评审
doi:10.3389/fnana.2015.00014
更新日期:2015-03-05 00:00:00
abstract::Why do neurons have dendritic spines? This question-the heart of what Yuste calls "the spine problem"-presupposes that why-questions of this sort have scientific answers: that empirical findings can favor or count against claims about why neurons have spines. Here we show how such questions can receive empirical answe...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2014.00095
更新日期:2014-09-10 00:00:00
abstract::Polysialic acid (polySia) is a complex sugar that in the nervous system appears mainly as a posttranslational modification of the neural cell adhesion molecule (NCAM). PolySia plays important roles during brain development, but also in its plasticity during adulthood. Two polysialyltransferases (polyST), ST8SIA2 and S...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2019.00006
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journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2015.00069
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journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章,评审
doi:10.3389/fnana.2014.00147
更新日期:2014-12-03 00:00:00
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journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2013.00018
更新日期:2013-06-26 00:00:00
abstract::Cortical calretinin (CR)-expressing interneurons represent a heterogeneous subpopulation of about 10-30% of GABAergic interneurons, which altogether total ca. 12-20% of all cortical neurons. In the rodent neocortex, CR cells display different somatodendritic morphologies ranging from bipolar to multipolar but the bipo...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章,评审
doi:10.3389/fnana.2014.00052
更新日期:2014-06-24 00:00:00
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journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2014.00127
更新日期:2014-11-12 00:00:00
abstract::The study of the links and interactions between development and motor learning has noticeable implications for the understanding and management of neurodevelopmental disorders. This is particularly relevant for the cerebellum which is critical for sensorimotor learning. The olivocerebellar pathway is a key pathway con...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2012.00001
更新日期:2012-01-23 00:00:00
abstract::We evaluated the neuropil distribution of the enzymes NADPH diaphorase (NADPH-d) and cytochrome oxidase (CO) in the spinal cord of the agouti, a medium-sized diurnal rodent, together with the distribution pattern and morphometrical characteristics of NADPH-d reactive neurons across different spinal segments. Neuropil ...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/neuro.05.002.2008
更新日期:2008-05-26 00:00:00
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journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章,评审
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abstract::A subset of monoamine neurons releases glutamate as a cotransmitter due to presence of the vesicular glutamate transporters VGLUT2 or VGLUT3. In addition to mediating vesicular loading of glutamate, it has been proposed that VGLUT3 enhances serotonin (5-HT) vesicular loading by the vesicular monoamine transporter (VMA...
journal_title:Frontiers in neuroanatomy
pub_type: 杂志文章
doi:10.3389/fnana.2016.00039
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