Whole Brain Imaging with Serial Two-Photon Tomography.

Abstract:

:Imaging entire mouse brains at submicron resolution has historically been a challenging undertaking and largely confined to the province of dedicated atlasing initiatives. This has limited systematic investigations into important areas of neuroscience, such as neural circuits, brain mapping and neurodegeneration. In this article, we describe in detail Serial Two-Photon (STP) tomography, a robust, reliable method for imaging entire brains with histological detail. We provide examples of how the basic methodology can be extended to other imaging modalities, such as Optical Coherence Tomography (OCT), in order to provide unique contrast mechanisms. Furthermore, we provide a survey of the research that STP tomography has enabled in the field of neuroscience, provide examples of how this technology enables quantitative whole brain studies, and discuss the current limitations of STP tomography-based approaches.

journal_name

Front Neuroanat

authors

Amato SP,Pan F,Schwartz J,Ragan TM

doi

10.3389/fnana.2016.00031

subject

Has Abstract

pub_date

2016-03-22 00:00:00

pages

31

issn

1662-5129

journal_volume

10

pub_type

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