Mapping Central Projection of Oxytocin Neurons in Unmated Mice Using Cre and Alkaline Phosphatase Reporter.

Abstract:

:Oxytocin, a neuropeptide and peptide hormone, is produced by neurons in the hypothalamus and released by the posterior pituitary to control breastfeeding and labor. Recent studies have revealed that oxytocin in the central nervous system is also involved in modulating social interaction. To understand the potential role and innervation pattern of oxytocin neurons before sexual interaction, here we used transgenic mice which have the Cre recombinase under the control of an endogenous oxytocin promoter and Cre-dependent human placental alkaline phosphatase (AP) reporter to label the oxytocin neurons in the naive mouse brain. Since AP is located on the membrane of oxytocin neurons, AP histochemistry staining enabled us to observe the fine axonal terminals and the innervation pattern of oxytocin neurons in the thick serial coronal brain slices. Here we show that the number of AP-labeled cells varies with staining reaction time and ranges from 30% of the oxytocin immune-positive cell count to slightly higher than the oxytocin immune-positive cell count. Using AP staining with extended reaction time, which may not label all oxytocin neurons, we confirmed many innervation targets of oxytocin neurons from the anterior olfactory nucleus, some cortex regions, the limbic system, the hypothalamus, and the hindbrain, while the cell bodies were exclusively located in the hypothalamus and the bed nucleus of the stria terminalis. Finally, we observe some individual variance at the olfactory area, isocortex, striatum, paraventricular nucleus of thalamus, locus coeruleus, and Barrington's nucleus.

journal_name

Front Neuroanat

authors

Liao PY,Chiu YM,Yu JH,Chen SK

doi

10.3389/fnana.2020.559402

subject

Has Abstract

pub_date

2020-10-19 00:00:00

pages

559402

issn

1662-5129

journal_volume

14

pub_type

杂志文章
  • Automatic Mitochondria Segmentation for EM Data Using a 3D Supervised Convolutional Network.

    abstract::Recent studies have supported the relation between mitochondrial functions and degenerative disorders related to ageing, such as Alzheimer's and Parkinson's diseases. Since these studies have exposed the need for detailed and high-resolution analysis of physical alterations in mitochondria, it is necessary to be able ...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2018.00092

    authors: Xiao C,Chen X,Li W,Li L,Wang L,Xie Q,Han H

    更新日期:2018-11-02 00:00:00

  • Distribution of vasotocin- and vasoactive intestinal peptide-like immunoreactivity in the brain of blue tit (Cyanistes coeruleus).

    abstract::Blue tits (Cyanistes coeruleus) are songbirds, used as model animals in numerous studies covering a wide field of research. Nevertheless, the distribution of neuropeptides in the brain of this avian species remains largely unknown. Here we present some of the first results on distribution of Vasotocine (AVT) and Vasoa...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2015.00090

    authors: Montagnese CM,Székely T,Csillag A,Zachar G

    更新日期:2015-07-14 00:00:00

  • Brain scaling in mammalian evolution as a consequence of concerted and mosaic changes in numbers of neurons and average neuronal cell size.

    abstract::Enough species have now been subject to systematic quantitative analysis of the relationship between the morphology and cellular composition of their brain that patterns begin to emerge and shed light on the evolutionary path that led to mammalian brain diversity. Based on an analysis of the shared and clade-specific ...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章,评审

    doi:10.3389/fnana.2014.00077

    authors: Herculano-Houzel S,Manger PR,Kaas JH

    更新日期:2014-08-11 00:00:00

  • Fast 3-D Imaging of Brain Organoids With a New Single-Objective Planar-Illumination Two-Photon Microscope.

    abstract::Human inducible pluripotent stem cells (hiPSCs) hold a large potential for disease modeling. hiPSC-derived human astrocyte and neuronal cultures permit investigations of neural signaling pathways with subcellular resolution. Combinatorial cultures, and three-dimensional (3-D) embryonic bodies (EBs) enlarge the scope o...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2019.00077

    authors: Rakotoson I,Delhomme B,Djian P,Deeg A,Brunstein M,Seebacher C,Uhl R,Ricard C,Oheim M

    更新日期:2019-08-20 00:00:00

  • Laminar Pattern of Projections Indicates the Hierarchical Organization of the Anterior Cingulate-Temporal Lobe Emotion System.

    abstract::The anterior cingulate cortex (ACC), surrounding the genu of the corpus callosum, plays important roles in emotional processing and is functionally divided into the dorsal, perigenual, and subgenual subregions (dACC, pgACC, and sgACC, respectively). Previous studies have suggested that the pgACC and sgACC have distinc...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2019.00074

    authors: Sakata H,Kim Y,Nejime M,Konoike N,Miyachi S,Nakamura K

    更新日期:2019-07-31 00:00:00

  • Optimization of 3D-Visualization of Micro-Anatomical Structures of the Human Inner Ear in Osmium Tetroxide Contrast Enhanced Micro-CT Scans.

    abstract::Introduction: Knowledge of the neuro-anatomical architecture of the inner ear contributes to the improvement and development of cochlear and vestibular implants. The present knowledge is mainly based on two-dimensional images (histology) or derived models that simplify the complexity of this architecture. This study i...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2018.00041

    authors: van den Boogert T,van Hoof M,Handschuh S,Glueckert R,Guinand N,Guyot JP,Kingma H,Perez-Fornos A,Seppen B,Johnson Chacko L,Schrott-Fischer A,van de Berg R

    更新日期:2018-05-22 00:00:00

  • Structural and Synaptic Organization of the Adult Reeler Mouse Somatosensory Neocortex: A Comparative Fine-Scale Electron Microscopic Study of Reeler With Wild Type Mice.

    abstract::The reeler mouse has been widely used to study various aspects of cortico- and synaptogenesis, but also as a model for several neurological and neurodegenerative disorders. In contrast to development, comparably little is known about the neuronal composition and synaptic organization of the adult reeler mouse neocorte...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2018.00080

    authors: Prume M,Rollenhagen A,Lübke JHR

    更新日期:2018-10-05 00:00:00

  • Structural Connectivity of the Anterior Cingulate Cortex, Claustrum, and the Anterior Insula of the Mouse.

    abstract::The claustrum is a narrow subcortical brain structure that resides between the striatum and insular cortex. The function of the claustrum is not fully described, and while our previous work supports a role for the claustrum in top-down cognitive control of action, other evidence suggests the claustrum may be involved ...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2018.00100

    authors: Qadir H,Krimmel SR,Mu C,Poulopoulos A,Seminowicz DA,Mathur BN

    更新日期:2018-11-26 00:00:00

  • The spine problem: finding a function for dendritic spines.

    abstract::Why do neurons have dendritic spines? This question-the heart of what Yuste calls "the spine problem"-presupposes that why-questions of this sort have scientific answers: that empirical findings can favor or count against claims about why neurons have spines. Here we show how such questions can receive empirical answe...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2014.00095

    authors: Malanowski S,Craver CF

    更新日期:2014-09-10 00:00:00

  • Neurons in the white matter of the adult human neocortex.

    abstract::The white matter (WM) of the adult human neocortex contains the so-called "interstitial neurons". They are most numerous in the superficial WM underlying the cortical gyri, and decrease in density toward the deep WM. They are morphologically heterogeneous. A subgroup of interstitial neurons display pyramidal-cell like...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/neuro.05.007.2009

    authors: Suárez-Solá ML,González-Delgado FJ,Pueyo-Morlans M,Medina-Bolívar OC,Hernández-Acosta NC,González-Gómez M,Meyer G

    更新日期:2009-06-09 00:00:00

  • Prepatterning and patterning of the thalamus along embryonic development of Xenopus laevis.

    abstract::Previous developmental studies of the thalamus (alar part of the diencephalic prosomere p2) have defined the molecular basis for the acquisition of the thalamic competence (preparttening), the subsequent formation of the secondary organizer in the zona limitans intrathalamica, and the early specification of two antero...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2015.00107

    authors: Bandín S,Morona R,González A

    更新日期:2015-08-10 00:00:00

  • Development of a highly sensitive immunohistochemical method to detect neurochemical molecules in formalin-fixed and paraffin-embedded tissues from autopsied human brains.

    abstract::Immunohistochemistry (IHC) is a valuable method for identifying discrete neurochemical molecules by the interaction of target antigens with validated antibodies tagged with a visible label (e.g., peroxidase). We have developed an immunostaining method that is highly sensitive in detection of neurochemical antigens. Ou...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2015.00022

    authors: Goto S,Morigaki R,Okita S,Nagahiro S,Kaji R

    更新日期:2015-03-03 00:00:00

  • The Golgi Apparatus of Neocortical Glial Cells During Hibernation in the Syrian Hamster.

    abstract::Hibernating mammals undergo torpor periods characterized by a general decrease in body temperature, metabolic rate, and brain activity accompanied by complex adaptive brain changes that appear to protect the brain from extreme conditions of hypoxia and low temperatures. These processes are accompanied by morphological...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2019.00092

    authors: León-Espinosa G,DeFelipe J,Muñoz A

    更新日期:2019-11-19 00:00:00

  • Pío del Río-Hortega: A Pioneer in the Pathology of Central Nervous System Tumors.

    abstract::The last 140 years have seen considerable advances in knowledge of central nervous system tumors. However, the main tumor types had already been described during the early years of the twentieth century. The studies of Dr. Pío del Río Hortega have been ones of the most exhaustive histology and cytology-based studies o...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章,评审

    doi:10.3389/fnana.2016.00013

    authors: Ramon Y Cajal Agüeras S

    更新日期:2016-03-01 00:00:00

  • A comparison of manual neuronal reconstruction from biocytin histology or 2-photon imaging: morphometry and computer modeling.

    abstract::Accurate 3D reconstruction of neurons is vital for applications linking anatomy and physiology. Reconstructions are typically created using Neurolucida after biocytin histology (BH). An alternative inexpensive and fast method is to use freeware such as Neuromantic to reconstruct from fluorescence imaging (FI) stacks a...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2014.00065

    authors: Blackman AV,Grabuschnig S,Legenstein R,Sjöström PJ

    更新日期:2014-07-11 00:00:00

  • Barriers in the brain: resolving dendritic spine morphology and compartmentalization.

    abstract::Dendritic spines are micron-sized protrusions that harbor the majority of excitatory synapses in the central nervous system. The head of the spine is connected to the dendritic shaft by a 50-400 nm thin membrane tube, called the spine neck, which has been hypothesized to confine biochemical and electric signals within...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章,评审

    doi:10.3389/fnana.2014.00142

    authors: Adrian M,Kusters R,Wierenga CJ,Storm C,Hoogenraad CC,Kapitein LC

    更新日期:2014-12-04 00:00:00

  • Whole Brain Imaging with Serial Two-Photon Tomography.

    abstract::Imaging entire mouse brains at submicron resolution has historically been a challenging undertaking and largely confined to the province of dedicated atlasing initiatives. This has limited systematic investigations into important areas of neuroscience, such as neural circuits, brain mapping and neurodegeneration. In t...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2016.00031

    authors: Amato SP,Pan F,Schwartz J,Ragan TM

    更新日期:2016-03-22 00:00:00

  • Septo-temporal distribution and lineage progression of hippocampal neurogenesis in a primate (Callithrix jacchus) in comparison to mice.

    abstract::Adult born neurons in the hippocampus show species-specific differences in their numbers, the pace of their maturation and their spatial distribution. Here, we present quantitative data on adult hippocampal neurogenesis in a New World primate, the common marmoset (Callithrix jacchus) that demonstrate parts of the line...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2015.00085

    authors: Amrein I,Nosswitz M,Slomianka L,van Dijk RM,Engler S,Klaus F,Raineteau O,Azim K

    更新日期:2015-06-29 00:00:00

  • Dogs Have the Most Neurons, Though Not the Largest Brain: Trade-Off between Body Mass and Number of Neurons in the Cerebral Cortex of Large Carnivoran Species.

    abstract::Carnivorans are a diverse group of mammals that includes carnivorous, omnivorous and herbivorous, domesticated and wild species, with a large range of brain sizes. Carnivory is one of several factors expected to be cognitively demanding for carnivorans due to a requirement to outsmart larger prey. On the other hand, l...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2017.00118

    authors: Jardim-Messeder D,Lambert K,Noctor S,Pestana FM,de Castro Leal ME,Bertelsen MF,Alagaili AN,Mohammad OB,Manger PR,Herculano-Houzel S

    更新日期:2017-12-12 00:00:00

  • RING Finger Protein 38 Is a Neuronal Protein in the Brain of Nile Tilapia, Oreochromis niloticus.

    abstract::Really interesting new gene (RING) finger protein is a type of zinc-binding motif found in a large family of functionally distinct proteins. RING finger proteins are involved in diverse cellular processes including apoptosis, DNA repair, cell cycle, signal transduction, tumour suppressor, vesicular transport, and pero...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2017.00072

    authors: Cham KL,Soga T,Parhar IS

    更新日期:2017-08-31 00:00:00

  • Synaptic connectivity of the cholinergic axons in the olfactory bulb of the cynomolgus monkey.

    abstract::The olfactory bulb (OB) of mammals receives cholinergic afferents from the horizontal limb of the diagonal band of Broca (HDB). At present, the synaptic connectivity of the cholinergic axons on the circuits of the OB has only been investigated in the rat. In this report, we analyze the synaptic connectivity of the cho...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2015.00028

    authors: Liberia T,Blasco-Ibáñez JM,Nácher J,Varea E,Lanciego JL,Crespo C

    更新日期:2015-03-17 00:00:00

  • Striatal signal transduction and drug addiction.

    abstract::Drug addiction is a severe neuropsychiatric disorder characterized by loss of control over motivated behavior. The need for effective treatments mandates a greater understanding of the causes and identification of new therapeutic targets for drug development. Drugs of abuse subjugate normal reward-related behavior to ...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2011.00060

    authors: Philibin SD,Hernandez A,Self DW,Bibb JA

    更新日期:2011-09-20 00:00:00

  • Spinogenesis and Pruning in the Anterior Ventral Inferotemporal Cortex of the Macaque Monkey: An Intracellular Injection Study of Layer III Pyramidal Cells.

    abstract::Pyramidal cells grow and mature at different rates among different cortical areas in the macaque monkey. In particular, differences across the areas have been reported in both the timing and magnitude of growth, branching, spinogenesis, and pruning in the basal dendritic trees of cells in layer III. Presently availabl...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2011.00042

    authors: Elston GN,Oga T,Okamoto T,Fujita I

    更新日期:2011-07-21 00:00:00

  • Huntington's Disease and Striatal Signaling.

    abstract::Huntington's Disease (HD) is the most frequent neurodegenerative disease caused by an expansion of polyglutamines (CAG). The main clinical manifestations of HD are chorea, cognitive impairment, and psychiatric disorders. The transmission of HD is autosomal dominant with a complete penetrance. HD has a single genetic c...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2011.00055

    authors: Roze E,Cahill E,Martin E,Bonnet C,Vanhoutte P,Betuing S,Caboche J

    更新日期:2011-08-23 00:00:00

  • Hypothesis on the dual origin of the Mammalian subplate.

    abstract::The development of the mammalian neocortex relies heavily on subplate. The proportion of this cell population varies considerably in different mammalian species. Subplate is almost undetectable in marsupials, forms a thin, but distinct layer in mouse and rat, a larger layer in carnivores and big-brained mammals as pig...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2011.00025

    authors: Montiel JF,Wang WZ,Oeschger FM,Hoerder-Suabedissen A,Tung WL,García-Moreno F,Holm IE,Villalón A,Molnár Z

    更新日期:2011-04-07 00:00:00

  • Congenital hypoplasia of the cerebellum: developmental causes and behavioral consequences.

    abstract::Over the last 60 years, the spotlight of research has periodically returned to the cerebellum as new techniques and insights have emerged. Because of its simple homogeneous structure, limited diversity of cell types and characteristic behavioral pathologies, the cerebellum is a natural home for studies of cell specifi...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章,评审

    doi:10.3389/fnana.2013.00029

    authors: Basson MA,Wingate RJ

    更新日期:2013-09-03 00:00:00

  • Functional connectivity estimation over large networks at cellular resolution based on electrophysiological recordings and structural prior.

    abstract::Despite many structural and functional aspects of the brain organization have been extensively studied in neuroscience, we are still far from a clear understanding of the intricate structure-function interactions occurring in the multi-layered brain architecture, where billions of different neurons are involved. Altho...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2014.00137

    authors: Ullo S,Nieus TR,Sona D,Maccione A,Berdondini L,Murino V

    更新日期:2014-11-20 00:00:00

  • Development of the adult neurogenic niche in the hippocampus of mice.

    abstract::When does adult hippocampal neurogenesis begin? We describe the development of the neurogenic niche in the subgranular zone (SGZ) of the hippocampal dentate gyrus. We did so from the perspective of the situation in the adult. Ontogeny of the dentate gyrus is complex and results in an ectopic neurogenic niche that life...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2015.00053

    authors: Nicola Z,Fabel K,Kempermann G

    更新日期:2015-05-07 00:00:00

  • Comparative Analysis of Nkx2.1 and Islet-1 Expression in Urodele Amphibians and Lungfishes Highlights the Pattern of Forebrain Organization in Early Tetrapods.

    abstract::Expression patterns of Nkx2.1 and Islet-1 (Isl1), which encode transcription factors that are key in the regionalization of the forebrain, were analyzed by combined immunohistochemical methods in young adult specimens of two lungfishes (Neoceratodus forsteri and Protopterus dolloi) and a urodele amphibian (Pleurodeles...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2018.00042

    authors: Moreno N,López JM,Morona R,Lozano D,Jiménez S,González A

    更新日期:2018-05-18 00:00:00

  • All brains are made of this: a fundamental building block of brain matter with matching neuronal and glial masses.

    abstract::How does the size of the glial and neuronal cells that compose brain tissue vary across brain structures and species? Our previous studies indicate that average neuronal size is highly variable, while average glial cell size is more constant. Measuring whole cell sizes in vivo, however, is a daunting task. Here we use...

    journal_title:Frontiers in neuroanatomy

    pub_type: 杂志文章

    doi:10.3389/fnana.2014.00127

    authors: Mota B,Herculano-Houzel S

    更新日期:2014-11-12 00:00:00