当前位置： SCI文献检索 > Retrovirology期刊下所有文献 > A purine-rich element in foamy virus pol regulates env splicing and gag/pol expression.

A purine-rich element in foamy virus pol regulates env splicing and gag/pol expression.

Abstract：

BACKGROUND:The foamy viral genome encodes four central purine-rich elements localized in the integrase-coding region of pol. Previously, we have shown that the first two of these RNA elements (A and B) are required for protease dimerization and activation. The D element functions as internal polypurine tract during reverse transcription. Peters et al., described the third element (C) as essential for gag expression suggesting that it might serve as an RNA export element for the unspliced genomic transcript. RESULTS:Here, we analysed env splicing and demonstrate that the described C element composed of three GAA repeats known to bind SR proteins regulates env splicing, thus balancing the amount of gag/pol mRNAs. Deletion of the C element effectively promotes a splice site switch from a newly identified env splice acceptor to the intrinsically strong downstream localised env 3' splice acceptor permitting complete splicing of almost all LTR derived transcripts. We provide evidence that repression of this env splice acceptor is a prerequisite for gag expression. This repression is achieved by the C element, resulting in impaired branch point recognition and SF1/mBBP binding. Separating the branch point from the overlapping purine-rich C element, by insertion of only 20 nucleotides, liberated repression and fully restored splicing to the intrinsically strong env 3' splice site. This indicated that the cis-acting element might repress splicing by blocking the recognition of essential splice site signals. CONCLUSIONS:The foamy viral purine-rich C element regulates splicing by suppressing the branch point recognition of the strongest env splice acceptor. It is essential for the formation of unspliced gag and singly spliced pol transcripts.

journal_name

Retrovirology

journal_title

Retrovirology

authors

Moschall R,Denk S,Erkelenz S,Schenk C,Schaal H,Bodem J

doi

10.1186/s12977-017-0337-6

subject

Has Abstract

pub_date

2017-02-06 00:00:00

pages

10

issue

1

issn

1742-4690

pii

10.1186/s12977-017-0337-6

journal_volume

14

pub_type

杂志文章

相关文献

Retrovirology文献大全
  • Clinical, virological and biochemical evidence supporting the association of HIV-1 reverse transcriptase polymorphism R284K and thymidine analogue resistance mutations M41L, L210W and T215Y in patients failing tenofovir/emtricitabine therapy.

    abstract：BACKGROUND:Thymidine analogue resistance mutations (TAMs) selected under treatment with nucleoside analogues generate two distinct genotypic profiles in the HIV-1 reverse transcriptase (RT): (i) TAM1: M41L, L210W and T215Y, and (ii) TAM2: D67N, K70R and K219E/Q, and sometimes T215F. Secondary mutations, including thumb...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-9-68

    authors： Betancor G,Garriga C,Puertas MC,Nevot M,Anta L,Blanco JL,Pérez-Elías MJ,de Mendoza C,Martínez MA,Martinez-Picado J,Menéndez-Arias L,Resistance Platform of the Spanish AIDS Research Network (ResRIS).,Iribarren JA,Caballero E,R

    更新日期：2012-08-13 00:00:00

  • HIV-1 Vif, APOBEC, and intrinsic immunity.

    abstract：:Members of the APOBEC family of cellular cytidine deaminases represent a recently identified group of proteins that provide immunity to infection by retroviruses and protect the cell from endogenous mobile retroelements. Yet, HIV-1 is largely immune to the intrinsic antiviral effects of APOBEC proteins because it enco...

    journal_title：Retrovirology

    pub_type： 杂志文章,评审

    doi：10.1186/1742-4690-5-51

    authors： Goila-Gaur R,Strebel K

    更新日期：2008-06-24 00:00:00

  • Modulation of HIV-1 infectivity and cyclophilin A-dependence by Gag sequence and target cell type.

    abstract：BACKGROUND:HIV-1 Gag proteins are essential for virion assembly and viral replication in newly infected cells. Gag proteins are also strong determinants of viral infectivity; immune escape mutations in the Gag capsid (CA) protein can markedly reduce viral fitness, and interactions of CA with host proteins such as cyclo...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-6-21

    authors： Matsuoka S,Dam E,Lecossier D,Clavel F,Hance AJ

    更新日期：2009-03-02 00:00:00

  • The co-receptor signaling model of HIV-1 pathogenesis in peripheral CD4 T cells.

    abstract：:HIV-mediated CD4 depletion is the hallmark of AIDS and is the most reliable predictor of disease progression. While HIV replication is associated with CD4 depletion in general, plasma viremia by itself predicts the rate of CD4 loss only minimally in untreated patients. To resolve this paradox, I hypothesize the existe...

    journal_title：Retrovirology

    pub_type： 社论

    doi：10.1186/1742-4690-6-41

    authors： Wu Y

    更新日期：2009-05-01 00:00:00

  • The silent defense: micro-RNA directed defense against HIV-1 replication.

    abstract：:MicroRNAs play critical role in regulating gene expression. MicroRNA profile of particular cell type bears the signature of cell type specific gene expression. Given that viral pathogens replicate by evading host defenses, research is now focused on the miRNA-regulated genes that critically regulate HIV-1 propagation ...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-4-26

    authors： Kumar A

    更新日期：2007-04-12 00:00:00

  • Molecular mechanisms by which HERV-K Gag interferes with HIV-1 Gag assembly and particle infectivity.

    abstract：BACKGROUND:Human endogenous retroviruses (HERVs), the remnants of ancient retroviral infections, constitute approximately 8% of human genomic DNA. Since HERV-K Gag expression is induced by HIV-1 Tat in T cells, induced HERV-K proteins could affect HIV-1 replication. Indeed, previously we showed that HERV-K Gag and HIV-...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-017-0351-8

    authors： Monde K,Terasawa H,Nakano Y,Soheilian F,Nagashima K,Maeda Y,Ono A

    更新日期：2017-04-26 00:00:00

  • An expanded model of HIV cell entry phenotype based on multi-parameter single-cell data.

    abstract：BACKGROUND:Entry of human immunodeficiency virus type 1 (HIV-1) into the host cell involves interactions between the viral envelope glycoproteins (Env) and the cellular receptor CD4 as well as a coreceptor molecule (most importantly CCR5 or CXCR4). Viral preference for a specific coreceptor (tropism) is in particular d...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-9-60

    authors： Bozek K,Eckhardt M,Sierra S,Anders M,Kaiser R,Kräusslich HG,Müller B,Lengauer T

    更新日期：2012-07-25 00:00:00

  • Identification of novel subgroup A variants with enhanced receptor binding and replicative capacity in primary isolates of anaemogenic strains of feline leukaemia virus.

    abstract：BACKGROUND:The development of anaemia in feline leukaemia virus (FeLV)-infected cats is associated with the emergence of a novel viral subgroup, FeLV-C. FeLV-C arises from the subgroup that is transmitted, FeLV-A, through alterations in the amino acid sequence of the receptor binding domain (RBD) of the envelope glycop...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-9-48

    authors： Stewart H,Adema KW,McMonagle EL,Hosie MJ,Willett BJ

    更新日期：2012-05-31 00:00:00

  • Tenofovir treatment augments anti-viral immunity against drug-resistant SIV challenge in chronically infected rhesus macaques.

    abstract：BACKGROUND:Emergence of drug-resistant strains of human immunodeficiency virus type 1 (HIV-1) is a major obstacle to successful antiretroviral therapy (ART) in HIV-infected patients. Whether antiviral immunity can augment ART by suppressing replication of drug-resistant HIV-1 in humans is not well understood, but can b...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-3-97

    authors： Metzner KJ,Binley JM,Gettie A,Marx P,Nixon DF,Connor RI

    更新日期：2006-12-21 00:00:00

  • Modulation of HIV-1 Gag NC/p1 cleavage efficiency affects protease inhibitor resistance and viral replicative capacity.

    abstract：BACKGROUND:Mutations in the substrate of HIV-1 protease, especially changes in the NC/p1 cleavage site, can directly contribute to protease inhibitor (PI) resistance and also compensate for defects in viral replicative capacity (RC) due to a drug resistant protease. These NC/p1 changes are known to enhance processing o...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-9-29

    authors： van Maarseveen NM,Andersson D,Lepšík M,Fun A,Schipper PJ,de Jong D,Boucher CA,Nijhuis M

    更新日期：2012-04-01 00:00:00

  • Small non-coding RNAs, mammalian cells, and viruses: regulatory interactions?

    abstract：:Recent findings suggest that mammalian cells can use small non-coding RNAs (ncRNA) to regulate physiological viral infections. Here, we comment on several lines of evidence that support this concept. We discuss how viruses may in turn protect, suppress, evade, modulate, or adapt to the host cell's ncRNA regulatory sch...

    journal_title：Retrovirology

    pub_type： 社论,评审

    doi：10.1186/1742-4690-4-74

    authors： Yeung ML,Benkirane M,Jeang KT

    更新日期：2007-10-15 00:00:00

  • Avian myeoloblastosis virus (AMV): only one side of the coin.

    abstract：:For many years, scientists and suppliers have refered to AMV-RT as the reverse transcriptase produced by the Avian Myelobalstosis Virus. This manuscript briefly reviews the molecular basis for biological dependence of AMV for the envelope and RT proteins that are produced by its natural helper the Myeloblastosis Assoc...

    journal_title：Retrovirology

    pub_type： 杂志文章,评审

    doi：10.1186/1742-4690-5-49

    authors： Perbal B

    更新日期：2008-06-16 00:00:00

  • Human TRIM5alpha mediated restriction of different HIV-1 subtypes and Lv2 sensitive and insensitive HIV-2 variants.

    abstract：:In order to characterize the antiviral activity of human TRIM5alpha in more detail human derived indicator cell lines over expressing wild type human TRIM5alpha were generated and challenged with HIV-1 and HIV-2 viruses pseudotyped with HIV envelope proteins in comparison to VSV-G pseudotyped particles. HIV envelope p...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-3-79

    authors： Kaumanns P,Hagmann I,Dittmar MT

    更新日期：2006-11-06 00:00:00

  • Incorporation of podoplanin into HIV released from HEK-293T cells, but not PBMC, is required for efficient binding to the attachment factor CLEC-2.

    abstract：BACKGROUND:Platelets are associated with HIV in the blood of infected individuals and might modulate viral dissemination, particularly if the virus is directly transmitted into the bloodstream. The C-type lectin DC-SIGN and the novel HIV attachment factor CLEC-2 are expressed by platelets and facilitate HIV transmissio...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-7-47

    authors： Chaipan C,Steffen I,Tsegaye TS,Bertram S,Glowacka I,Kato Y,Schmökel J,Münch J,Simmons G,Gerardy-Schahn R,Pöhlmann S

    更新日期：2010-05-19 00:00:00

  • Preferences for the selection of unique tRNA primers revealed from analysis of HIV-1 replication in peripheral blood mononuclear cells.

    abstract：BACKGROUND:All human immunodeficiency virus (HIV-1) uses a host tRNALys,3 as the primer for reverse transcription. The tRNALys,3 is bound to a region on the HIV-1 genome, the primer-binding site (PBS), that is complementary to the 18 terminal nucleotides of tRNALys,3. How HIV-1 selects the tRNA from the intracellular m...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-2-21

    authors： Moore-Rigdon KL,Kosloff BR,Kirkman RL,Morrow CD

    更新日期：2005-03-24 00:00:00

  • Host mRNA decay proteins influence HIV-1 replication and viral gene expression in primary monocyte-derived macrophages.

    abstract：BACKGROUND:Mammalian cells harbour RNA quality control and degradative machineries such as nonsense-mediated mRNA decay that target cellular mRNAs for clearance from the cell to avoid aberrant gene expression. The role of the host mRNA decay pathways in macrophages in the context of human immunodeficiency virus type 1 ...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-019-0465-2

    authors： Rao S,Amorim R,Niu M,Breton Y,Tremblay MJ,Mouland AJ

    更新日期：2019-02-07 00:00:00

  • Stabilization of the β-hairpin in Mason-Pfizer monkey virus capsid protein- a critical step for infectivity.

    abstract：BACKGROUND:Formation of a mature core is a crucial event for infectivity of retroviruses such as Mason-Pfizer monkey virus (M-PMV). The process is triggered by proteolytic cleavage of the polyprotein precursor Gag, which releases matrix, capsid (CA), and nucleocapsid proteins. Once released, CA assembles to form a matu...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-014-0094-8

    authors： Obr M,Hadravová R,DoleŽal M,KříŽová I,Papoušková V,Zídek L,Hrabal R,Ruml T,Rumlová M

    更新日期：2014-10-30 00:00:00

  • The Infectious Diseases BioBank at King's College London: archiving samples from patients infected with HIV to facilitate translational research.

    abstract：:The King's College London (KCL) Infectious Diseases BioBank opened in 2007 and collects peripheral venous blood (PVB) from individuals infected with pathogens including human immunodeficiency virus (HIV). PVBs are fractionated into plasmas, lymphocytes and DNA and are then frozen. All donations are from subjects who h...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-6-98

    authors： Williams R,Mant C,Cason J

    更新日期：2009-11-03 00:00:00

  • Caveolin-1 reduces HIV-1 infectivity by restoration of HIV Nef mediated impairment of cholesterol efflux by apoA-I.

    abstract：BACKGROUND:HIV infection results in inhibited cholesterol efflux by apolipoprotein A-I (apoA-I) in macrophages, and this impairment involves Nef mediated down-regulation and redistribution of ATP-binding cassette transporter A1 (ABCA-1). We investigated the effect of caveolin-1 (Cav-1) on the cholesterol efflux by apoA...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-9-85

    authors： Lin S,Nadeau PE,Wang X,Mergia A

    更新日期：2012-10-15 00:00:00

  • Crystal structure of an FIV/HIV chimeric protease complexed with the broad-based inhibitor, TL-3.

    abstract：:We have obtained the 1.7 A crystal structure of FIV protease (PR) in which 12 critical residues around the active site have been substituted with the structurally equivalent residues of HIV PR (12X FIV PR). The chimeric PR was crystallized in complex with the broad-based inhibitor TL-3, which inhibits wild type FIV an...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-4-1

    authors： Heaslet H,Lin YC,Tam K,Torbett BE,Elder JH,Stout CD

    更新日期：2007-01-09 00:00:00

  • Discovery of a diaminoquinoxaline benzenesulfonamide antagonist of HIV-1 Nef function using a yeast-based phenotypic screen.

    abstract：BACKGROUND:HIV-1 Nef is a viral accessory protein critical for AIDS progression. Nef lacks intrinsic catalytic activity and binds multiple host cell signaling proteins, including Hck and other Src-family tyrosine kinases. Nef binding induces constitutive Hck activation that may contribute to HIV pathogenesis by promoti...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-10-135

    authors： Trible RP,Narute P,Emert-Sedlak LA,Alvarado JJ,Atkins K,Thomas L,Kodama T,Yanamala N,Korotchenko V,Day BW,Thomas G,Smithgall TE

    更新日期：2013-11-14 00:00:00

  • The S40 residue in HIV-1 Gag p6 impacts local and distal budding determinants, revealing additional late domain activities.

    abstract：BACKGROUND:HIV-1 budding is directed primarily by two motifs in Gag p6 designated as late domain-1 and -2 that recruit ESCRT machinery by binding Tsg101 and Alix, respectively, and by poorly characterized determinants in the capsid (CA) domain. Here, we report that a conserved Gag p6 residue, S40, impacts budding media...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-10-143

    authors： Watanabe SM,Chen MH,Khan M,Ehrlich L,Kemal KS,Weiser B,Shi B,Chen C,Powell M,Anastos K,Burger H,Carter CA

    更新日期：2013-11-21 00:00:00

  • HLA-B*35 as a new marker for susceptibility to human T-cell lymphotropic virus type 1 (HTLV-1) Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP) in patients living in Argentina.

    abstract：BACKGROUND:Human T-cell lymphotropic virus type 1 (HTLV-1) is the etiological agent of HTLV associated myelopathy/ Tropical Spastic Paraparesis (HAM/TSP) and Adult T cell leukemia/lymphoma (ATLL), in around 2-5% of the infected individuals. Host genetic background might play a role in disease progression. Several previ...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-020-00536-y

    authors： Benencio P,Fraile Gonzalez SA,Ducasa N,Page K,Berini CA,Biglione MM

    更新日期：2020-09-03 00:00:00

  • The role of integration and clonal expansion in HIV infection: live long and prosper.

    abstract：:Integration of viral DNA into the host genome is a central event in the replication cycle and the pathogenesis of retroviruses, including HIV. Although most cells infected with HIV are rapidly eliminated in vivo, HIV also infects long-lived cells that persist during combination antiretroviral therapy (cART). Cells wit...

    journal_title：Retrovirology

    pub_type： 杂志文章,评审

    doi：10.1186/s12977-018-0448-8

    authors： Anderson EM,Maldarelli F

    更新日期：2018-10-23 00:00:00

  • Persistent resistance to HIV-1 infection in CD4 T cells from exposed uninfected Vietnamese individuals is mediated by entry and post-entry blocks.

    abstract：BACKGROUND:We have previously reported that CD4 T cells from some exposed uninfected (EU) Vietnamese intravenous drug users are relatively resistant to HIV infection in vitro. Here, we further characterized the restriction of viral replication in CD4 T cells from five EUs and assessed its persistence in serial samples....

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-3-81

    authors： Sáez-Cirión A,Versmisse P,Truong LX,Chakrabarti LA,Carpentier W,Barré-Sinoussi F,Scott-Algara D,Pancino G

    更新日期：2006-11-08 00:00:00

  • Induction of p21(CIP1/WAF1) expression by human T-lymphotropic virus type 1 Tax requires transcriptional activation and mRNA stabilization.

    abstract：:HTLV-1 Tax can induce senescence by up-regulating the levels of cyclin-dependent kinase inhibitors p21(CIP1/WAF1) and p27(KIP1). Tax increases p27(KIP1) protein stability by activating the anaphase promoting complex/cyclosome (APC/C) precociously, causing degradation of Skp2 and inactivation of SCF(Skp2), the E3 ligas...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-6-35

    authors： Zhang L,Zhi H,Liu M,Kuo YL,Giam CZ

    更新日期：2009-04-08 00:00:00

  • Analysis of herpes simplex type 1 gB, gD, and gH/gL on production of infectious HIV-1: HSV-1 gD restricts HIV-1 by exclusion of HIV-1 Env from maturing viral particles.

    abstract：BACKGROUND:We previously showed that the gM of HSV-1 could restrict the release of infectious HIV-1 from cells. In this study, we analyzed if the four HSV-1 glycoproteins (gD, gB, and gH/gL), which are the minimum glycoproteins required for HSV-1 entry, restricted the release of infectious HIV-1. RESULTS:Of these four...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-019-0470-5

    authors： Polpitiya Arachchige S,Henke W,Kalamvoki M,Stephens EB

    更新日期：2019-04-02 00:00:00

  • IFITM proteins are incorporated onto HIV-1 virion particles and negatively imprint their infectivity.

    abstract：BACKGROUND:Interferon induced transmembrane proteins 1, 2 and 3 (IFITMs) belong to a family of highly related antiviral factors that have been shown to interfere with a large spectrum of viruses including Filoviruses, Coronaviruses, Influenza virus, Dengue virus and HIV-1. In all these cases, the reported mechanism of ...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-014-0103-y

    authors： Tartour K,Appourchaux R,Gaillard J,Nguyen XN,Durand S,Turpin J,Beaumont E,Roch E,Berger G,Mahieux R,Brand D,Roingeard P,Cimarelli A

    更新日期：2014-11-25 00:00:00

  • Correction to: HIV-1 IN/Pol recruits LEDGF/p75 into viral particles.

    abstract：:An amendment to this paper has been published and can be accessed via the original article. ...

    journal_title：Retrovirology

    pub_type： 杂志文章,已发布勘误

    doi：10.1186/s12977-020-00531-3

    authors： Desimmie BA,Weydert C,Schrijvers R,Vets S,Demeulemeester J,Proost P,Paron I,De Rijck J,Mast J,Bannert N,Gijsbers R,Christ F,Debyser Z

    更新日期：2020-07-29 00:00:00

  • Perinatal HIV-1 transmission: Fc gamma receptor variability associates with maternal infectiousness and infant susceptibility.

    abstract：BACKGROUND:Accumulating data suggest that immune effector functions mediated through the Fc portion of HIV-1-specific immunoglobulin G (IgG) are a key component of HIV-1 protective immunity, affecting both disease progression and HIV-1 acquisition. Through studying Fc gamma receptor (FcγR) variants known to alter IgG F...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-016-0272-y

    authors： Lassaunière R,Musekiwa A,Gray GE,Kuhn L,Tiemessen CT

    更新日期：2016-06-10 00:00:00