当前位置： SCI文献检索 > Retrovirology期刊下所有文献 > Stabilization of the β-hairpin in Mason-Pfizer monkey virus capsid protein- a critical step for infectivity.

Stabilization of the β-hairpin in Mason-Pfizer monkey virus capsid protein- a critical step for infectivity.

Abstract：

BACKGROUND:Formation of a mature core is a crucial event for infectivity of retroviruses such as Mason-Pfizer monkey virus (M-PMV). The process is triggered by proteolytic cleavage of the polyprotein precursor Gag, which releases matrix, capsid (CA), and nucleocapsid proteins. Once released, CA assembles to form a mature core - a hexameric lattice protein shell that protects retroviral genomic RNA. Subtle conformational changes within CA induce the transition from the immature lattice to the mature lattice. Upon release from the precursor, the initially unstructured N-terminus of CA is refolded to form a β-hairpin stabilized by a salt bridge between the N-terminal proline and conserved aspartate. Although the crucial role of the β-hairpin in the mature core assembly has been confirmed, its precise structural function remains poorly understood. RESULTS:Based on a previous NMR analysis of the N-terminal part of M-PMV CA, which suggested the role of additional interactions besides the proline-aspartate salt bridge in stabilization of the β-hairpin, we introduced a series of mutations into the CA sequence. The effect of the mutations on virus assembly and infectivity was analyzed. In addition, the structural consequences of selected mutations were determined by NMR spectroscopy. We identified a network of interactions critical for proper formation of the M-PMV core. This network involves residue R14, located in the N-terminal β-hairpin; residue W52 in the loop connecting helices 2 and 3; and residues Q113, Q115, and Y116 in helix 5. CONCLUSION:Combining functional and structural analyses, we identified a network of supportive interactions that stabilize the β-hairpin in mature M-PMV CA.

journal_name

Retrovirology

journal_title

Retrovirology

authors

Obr M,Hadravová R,DoleŽal M,KříŽová I,Papoušková V,Zídek L,Hrabal R,Ruml T,Rumlová M

doi

10.1186/s12977-014-0094-8

subject

Has Abstract

pub_date

2014-10-30 00:00:00

pages

94

issn

1742-4690

pii

s12977-014-0094-8

journal_volume

11

pub_type

杂志文章

相关文献

Retrovirology文献大全
  • Evolution and gene capture in ancient endogenous retroviruses - insights from the crocodilian genomes.

    abstract：BACKGROUND:Crocodilians are thought to be hosts to a diverse and divergent complement of endogenous retroviruses (ERVs) but a comprehensive investigation is yet to be performed. The recent sequencing of three crocodilian genomes provides an opportunity for a more detailed and accurate representation of the ERV diversit...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-014-0071-2

    authors： Chong AY,Kojima KK,Jurka J,Ray DA,Smit AF,Isberg SR,Gongora J

    更新日期：2014-12-12 00:00:00

  • Enhanced antibody-mediated neutralization of HIV-1 variants that are resistant to fusion inhibitors.

    abstract：BACKGROUND:HIV-1 typically develops resistance to any single antiretroviral agent. Combined anti-retroviral therapy to reduce drug-resistance development is necessary to control HIV-1 infection. Here, to assess the utility of a combination of antibody and fusion inhibitor treatments, we investigated the potency of mono...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-016-0304-7

    authors： Alam M,Kuwata T,Shimura K,Yokoyama M,Ramirez Valdez KP,Tanaka K,Maruta Y,Oishi S,Fujii N,Sato H,Matsuoka M,Matsushita S

    更新日期：2016-09-27 00:00:00

  • Incorporation of podoplanin into HIV released from HEK-293T cells, but not PBMC, is required for efficient binding to the attachment factor CLEC-2.

    abstract：BACKGROUND:Platelets are associated with HIV in the blood of infected individuals and might modulate viral dissemination, particularly if the virus is directly transmitted into the bloodstream. The C-type lectin DC-SIGN and the novel HIV attachment factor CLEC-2 are expressed by platelets and facilitate HIV transmissio...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-7-47

    authors： Chaipan C,Steffen I,Tsegaye TS,Bertram S,Glowacka I,Kato Y,Schmökel J,Münch J,Simmons G,Gerardy-Schahn R,Pöhlmann S

    更新日期：2010-05-19 00:00:00

  • Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line.

    abstract：:The mouse macrophage-like cell line RAW264.7, the most commonly used mouse macrophage cell line in medical research, was originally reported to be free of replication-competent murine leukemia virus (MuLV) despite its origin in a tumor induced by Abelson MuLV containing Moloney MuLV as helper virus. As currently avail...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-5-1

    authors： Hartley JW,Evans LH,Green KY,Naghashfar Z,Macias AR,Zerfas PM,Ward JM

    更新日期：2008-01-04 00:00:00

  • PCR mediated recombination impacts the analysis of hepatitis B Virus covalently closed circular DNA.

    abstract：BACKGROUND:The replication of HBV involves the production of covalently closed circular DNA (cccDNA) from the HBV genome through the repair of virion relaxed circular DNA (rcDNA) in the virion. As cccDNA is the transcription template for HBV genomes, it needs to be eliminated from hepatocytes if the eradication of chro...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-016-0318-1

    authors： Suspène R,Thiers V,Vartanian JP,Wain-Hobson S

    更新日期：2016-12-20 00:00:00

  • HIV-1 Rev oligomerization is not obligatory in the presence of an extra basic domain.

    abstract：BACKGROUND:The HIV-1 Rev regulatory protein binds as an oligomeric complex to viral RNA mediating nuclear export of incompletely spliced and non-spliced viral mRNAs encoding the viral structural proteins. However, the biological significance of the obligatory complex formation of Rev upon the viral RNA is unclear. RES...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-2-39

    authors： Furnes C,Arnesen T,Askjaer P,Kjems J,Szilvay AM

    更新日期：2005-06-10 00:00:00

  • Primate lentiviral Nef proteins deregulate T-cell development by multiple mechanisms.

    abstract：BACKGROUND:A nef gene is present in all primate lentiviral genomes and is important for high viral loads and progression to AIDS in human or experimental macaque hosts of HIV or SIV, respectively. In these hosts, infection of the thymus results in a decreased output of naive T cells that may contribute to the developme...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-10-137

    authors： Van Nuffel A,Ariën KK,Stove V,Schindler M,O'Neill E,Schmökel J,Van de Walle I,Naessens E,Vanderstraeten H,Van Landeghem K,Taghon T,Pulkkinen K,Saksela K,Garcia JV,Fackler OT,Kirchhoff F,Verhasselt B

    更新日期：2013-11-15 00:00:00

  • NHEJ pathway is involved in post-integrational DNA repair due to Ku70 binding to HIV-1 integrase.

    abstract：BACKGROUND:HIV-1 integration results in genomic DNA gaps that are repaired by cellular DNA repair pathways. This step of the lentiviral life cycle remains poorly understood despite its crucial importance for successful replication. We and others reported that Ku70 protein of the non-homologous end joining pathway (NHEJ...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-019-0492-z

    authors： Knyazhanskaya E,Anisenko A,Shadrina O,Kalinina A,Zatsepin T,Zalevsky A,Mazurov D,Gottikh M

    更新日期：2019-11-06 00:00:00

  • Genome-wide association studies on HIV susceptibility, pathogenesis and pharmacogenomics.

    abstract：:Susceptibility to HIV-1 and the clinical course after infection show a substantial heterogeneity between individuals. Part of this variability can be attributed to host genetic variation. Initial candidate gene studies have revealed interesting host factors that influence HIV infection, replication and pathogenesis. R...

    journal_title：Retrovirology

    pub_type： 杂志文章,评审

    doi：10.1186/1742-4690-9-70

    authors： van Manen D,van 't Wout AB,Schuitemaker H

    更新日期：2012-08-24 00:00:00

  • microRNAs in viral oncogenesis.

    abstract：:MicroRNAs are a recently discovered class of small noncoding functional RNAs. These molecules mediate post-transcriptional regulation of gene expression in a sequence specific manner. MicroRNAs are now known to be key players in a variety of biological processes and have been shown to be deregulated in a number of can...

    journal_title：Retrovirology

    pub_type： 社论,评审

    doi：10.1186/1742-4690-4-82

    authors： Scaria V,Jadhav V

    更新日期：2007-11-24 00:00:00

  • Interferon regulatory factor 4 as a therapeutic target in adult T-cell leukemia lymphoma.

    abstract：BACKGROUND:Adult T-cell leukemia lymphoma (ATLL) is a chemotherapy-resistant malignancy with a median survival of less than one year that will afflict between one hundred thousand and one million individuals worldwide who are currently infected with human T-cell leukemia virus type 1. Recurrent somatic mutations in hos...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-020-00535-z

    authors： Rauch DA,Olson SL,Harding JC,Sundaramoorthi H,Kim Y,Zhou T,MacLeod AR,Challen G,Ratner L

    更新日期：2020-08-28 00:00:00

  • Digital PCR as a tool to measure HIV persistence.

    abstract：:Although antiretroviral therapy is able to suppress HIV replication in infected patients, the virus persists and rebounds when treatment is stopped. In order to find a cure that can eradicate the latent reservoir, one must be able to quantify the persisting virus. Traditionally, HIV persistence studies have used real-...

    journal_title：Retrovirology

    pub_type： 杂志文章,评审

    doi：10.1186/s12977-018-0399-0

    authors： Rutsaert S,Bosman K,Trypsteen W,Nijhuis M,Vandekerckhove L

    更新日期：2018-01-30 00:00:00

  • Gene expression profiling of microglia infected by a highly neurovirulent murine leukemia virus: implications for neuropathogenesis.

    abstract：BACKGROUND:Certain murine leukemia viruses (MLVs) are capable of inducing progressive spongiform motor neuron disease in susceptible mice upon infection of the central nervous system (CNS). The major CNS parenchymal target of these neurovirulent retroviruses (NVs) are the microglia, whose infection is largely coinciden...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-3-26

    authors： Dimcheff DE,Volkert LG,Li Y,DeLucia AL,Lynch WP

    更新日期：2006-05-12 00:00:00

  • Inhibition of Tat activity by the HEXIM1 protein.

    abstract：BACKGROUND:The positive transcription elongation factor b (P-TEFb) composed by CDK9/CyclinT1 subunits is a dedicated co-factor of HIV transcriptional transactivator Tat protein. Transcription driven by the long terminal repeat (LTR) of HIV involves formation of a quaternary complex between P-TEFb, Tat and the TAR eleme...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-2-42

    authors： Fraldi A,Varrone F,Napolitano G,Michels AA,Majello B,Bensaude O,Lania L

    更新日期：2005-07-02 00:00:00

  • Natural APOBEC3C variants can elicit differential HIV-1 restriction activity.

    abstract：BACKGROUND:The APOBEC3 (A3) family of DNA cytosine deaminases provides an innate barrier to infection by retroviruses including HIV-1. A total of five enzymes, A3C, A3D, A3F, A3G and A3H, are degraded by the viral accessory protein Vif and expressed at high levels in CD4+ T cells, the primary reservoir for HIV-1 replic...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-018-0459-5

    authors： Anderson BD,Ikeda T,Moghadasi SA,Martin AS,Brown WL,Harris RS

    更新日期：2018-12-17 00:00:00

  • Longitudinal analysis of subtype C envelope tropism for memory CD4+ T cell subsets over the first 3 years of untreated HIV-1 infection.

    abstract：BACKGROUND:HIV-1 infects a wide range of CD4+ T cells with different phenotypic properties and differing expression levels of entry coreceptors. We sought to determine the viral tropism of subtype C (C-HIV) Envelope (Env) clones for different CD4+ T cell subsets and whether tropism changes during acute to chronic disea...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-020-00532-2

    authors： Gartner MJ,Gorry PR,Tumpach C,Zhou J,Dantanarayana A,Chang JJ,Angelovich TA,Ellenberg P,Laumaea AE,Nonyane M,Moore PL,Lewin SR,Churchill MJ,Flynn JK,Roche M

    更新日期：2020-08-06 00:00:00

  • The challenge of describing the epidemiology of HTLV in the Amazon region of Brazil.

    abstract：:HTLV-1 was the first described human retrovirus and was soon found to be associated with severe clinical diseases, including a devastating lymphoma/leukemia and other inflammatory diseases. Although HTLV-2 is not usually pathogenic, it is widely distributed among native Indian populations in Brazil, particularly in th...

    journal_title：Retrovirology

    pub_type： 杂志文章,评审

    doi：10.1186/s12977-020-0512-z

    authors： Ishak R,de Oliveira Guimarães Ishak M,Vallinoto ACR

    更新日期：2020-02-14 00:00:00

  • Are anti-HIV IgAs good guys or bad guys?

    abstract：:An estimated 90% of all HIV transmissions occur mucosally. Immunoglobulin A (IgA) molecules are important components of mucosal fluids. In a vaccine efficacy study, in which virosomes displaying HIV gp41 antigens protected most rhesus monkeys (RMs) against simian-human immunodeficiency virus (SHIV), protection correla...

    journal_title：Retrovirology

    pub_type： 杂志文章,评审

    doi：10.1186/s12977-014-0109-5

    authors： Zhou M,Ruprecht RM

    更新日期：2014-12-14 00:00:00

  • Evidence against a role for jaagsiekte sheep retrovirus in human lung cancer.

    abstract：BACKGROUND:Jaagsiekte sheep retrovirus (JSRV) causes a contagious lung cancer in sheep and goats that can be transmitted by aerosols produced by infected animals. Virus entry into cells is initiated by binding of the viral envelope (Env) protein to a specific cell-surface receptor, Hyal2. Unlike almost all other retrov...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-017-0329-6

    authors： Miller AD,De Las Heras M,Yu J,Zhang F,Liu SL,Vaughan AE,Vaughan TL,Rosadio R,Rocca S,Palmieri G,Goedert JJ,Fujimoto J,Wistuba II

    更新日期：2017-01-20 00:00:00

  • Distinct gene expression signatures induced by viral transactivators of different HTLV-1 subgroups that confer a different risk of HAM/TSP.

    abstract：BACKGROUND:Among human T cell leukemia virus type 1 (HTLV-1)-infected individuals, there is an association between HTLV-1 tax subgroups (subgroup-A or subgroup-B) and the risk of HAM/TSP in the Japanese population. To investigate the role of HTLV-1 subgroups in viral pathogenesis, we studied the functional difference i...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-018-0454-x

    authors： Naito T,Yasunaga JI,Mitobe Y,Shirai K,Sejima H,Ushirogawa H,Tanaka Y,Nakamura T,Hanada K,Fujii M,Matsuoka M,Saito M

    更新日期：2018-11-06 00:00:00

  • SIVdrl detection in captive mandrills: are mandrills infected with a third strain of simian immunodeficiency virus?

    abstract：:A pol-fragment of simian immunodeficiency virus (SIV) that is highly related to SIVdrl-pol from drill monkeys (Mandrillus leucophaeus) was detected in two mandrills (Mandrillus sphinx) from Amsterdam Zoo. These captivity-born mandrills had never been in contact with drill monkeys, and were unlikely to be hybrids. Thei...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-1-36

    authors： van der Kuyl AC,van den Burg R,Hoyer MJ,Gruters RA,Osterhaus AD,Berkhout B

    更新日期：2004-11-01 00:00:00

  • Akt inhibitors as an HIV-1 infected macrophage-specific anti-viral therapy.

    abstract：BACKGROUND:Unlike CD4+ T cells, HIV-1 infected macrophages exhibit extended life span even upon stress, consistent with their in vivo role as long-lived HIV-1 reservoirs. RESULTS:Here, we demonstrate that PI3K/Akt inhibitors, including clinically available Miltefosine, dramatically reduced HIV-1 production from long-l...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-5-11

    authors： Chugh P,Bradel-Tretheway B,Monteiro-Filho CM,Planelles V,Maggirwar SB,Dewhurst S,Kim B

    更新日期：2008-01-31 00:00:00

  • Ubiquitination and sumoylation of the HTLV-2 Tax-2B protein regulate its NF-κB activity: a comparative study with the HTLV-1 Tax-1 protein.

    abstract：BACKGROUND:Retroviruses HTLV-1 and HTLV-2 have homologous genomic structures but differ significantly in pathogenicity. HTLV-1 is associated with Adult T cell Leukemia (ATL), whereas infection by HTLV-2 has no association with neoplasia. Transformation of T lymphocytes by HTLV-1 is linked to the capacity of its oncopro...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-9-102

    authors： Turci M,Lodewick J,Di Gennaro G,Rinaldi AS,Marin O,Diani E,Sampaio C,Bex F,Bertazzoni U,Romanelli MG

    更新日期：2012-12-07 00:00:00

  • Memories of John N. Brady: scientist, mentor and friend.

    abstract：:Friends and colleagues remember John N. Brady, Ph.D., Chief of the Virus Tumor Biology Section of the Laboratory of Cellular Oncology, who died much too young at the age of 57 on April 27, 2009 of colon cancer. John grew up in Illinois and received his Ph.D. with Dr. Richard Consigli at Kansas State University studyin...

    journal_title：Retrovirology

    pub_type： 传,社论,历史文章

    doi：10.1186/1742-4690-6-48

    authors： Pise-Masison CA,Marriott SJ

    更新日期：2009-05-19 00:00:00

  • Generation and validation of a highly sensitive bioluminescent HIV-1 reporter vector that simplifies measurement of virus release.

    abstract：BACKGROUND:The continued persistence of HIV-1 as a public health concern due to the lack of a cure calls for the development of new tools for studying replication of the virus. Here, we used NanoLuc, a small and extremely bright luciferase protein, to develop an HIV-1 bioluminescent reporter virus that simplifies funct...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-020-00521-5

    authors： Kirui J,Freed EO

    更新日期：2020-05-19 00:00:00

  • The distribution of insertionally polymorphic endogenous retroviruses in breast cancer patients and cancer-free controls.

    abstract：BACKGROUND:Integration of retroviral DNA into a germ cell can result in a provirus that is transmitted vertically to the host's offspring. In humans, such endogenous retroviruses (HERVs) comprise >8% of the genome. The HERV-K(HML-2) proviruses consist of ~90 elements related to mouse mammary tumor virus, which causes b...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/s12977-014-0062-3

    authors： Wildschutte JH,Ram D,Subramanian R,Stevens VL,Coffin JM

    更新日期：2014-08-12 00:00:00

  • A comparison of the ability of rilpivirine (TMC278) and selected analogues to inhibit clinically relevant HIV-1 reverse transcriptase mutants.

    abstract：BACKGROUND:The recently approved anti-AIDS drug rilpivirine (TMC278, Edurant) is a nonnucleoside inhibitor (NNRTI) that binds to reverse transcriptase (RT) and allosterically blocks the chemical step of DNA synthesis. In contrast to earlier NNRTIs, rilpivirine retains potency against well-characterized, clinically rele...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-9-99

    authors： Johnson BC,Pauly GT,Rai G,Patel D,Bauman JD,Baker HL,Das K,Schneider JP,Maloney DJ,Arnold E,Thomas CJ,Hughes SH

    更新日期：2012-12-05 00:00:00

  • Identification of novel subgroup A variants with enhanced receptor binding and replicative capacity in primary isolates of anaemogenic strains of feline leukaemia virus.

    abstract：BACKGROUND:The development of anaemia in feline leukaemia virus (FeLV)-infected cats is associated with the emergence of a novel viral subgroup, FeLV-C. FeLV-C arises from the subgroup that is transmitted, FeLV-A, through alterations in the amino acid sequence of the receptor binding domain (RBD) of the envelope glycop...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-9-48

    authors： Stewart H,Adema KW,McMonagle EL,Hosie MJ,Willett BJ

    更新日期：2012-05-31 00:00:00

  • Efficient trapping of HIV-1 envelope protein by hetero-oligomerization with an N-helix chimera.

    abstract：BACKGROUND:The N-heptad repeat region of the HIV-1 Transmembrane Envelope protein is a trimerization domain that forms part of a "six helix bundle" crucial to Envelope-mediated membrane fusion. N-heptad repeat peptides have been used as extracellular reagents to inhibit virus fusion. RESULTS:When expressed intracellul...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-2-51

    authors： Ou W,Silver J

    更新日期：2005-08-10 00:00:00

  • Higher levels of Zidovudine resistant HIV in the colon compared to blood and other gastrointestinal compartments in HIV infection.

    abstract：BACKGROUND:The gut-associated lymphoid tissue (GALT) is the largest lymphoid organ infected by human immunodeficiency virus type 1 (HIV-1). It serves as a viral reservoir and host-pathogen interface in infection. This study examined whether different parts of the gut and peripheral blood lymphocytes (PBL) contain diffe...

    journal_title：Retrovirology

    pub_type： 杂志文章

    doi：10.1186/1742-4690-7-74

    authors： van Marle G,Church DL,Nunweiler KD,Cannon K,Wainberg MA,Gill MJ

    更新日期：2010-09-13 00:00:00