Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line.


:The mouse macrophage-like cell line RAW264.7, the most commonly used mouse macrophage cell line in medical research, was originally reported to be free of replication-competent murine leukemia virus (MuLV) despite its origin in a tumor induced by Abelson MuLV containing Moloney MuLV as helper virus. As currently available, however, we find that it produces significant levels of ecotropic MuLV with the biologic features of the Moloney isolate and also MuLV of the polytropic or MCF class. Newborn mice developed lymphoma following inoculation with the MuLV mixture expressed by these cells. These findings should be considered in interpretation of increasingly widespread use of these cells for propagation of other viruses, studies of biological responses to virus infection and use in RNA interference and cell signalling studies.






Hartley JW,Evans LH,Green KY,Naghashfar Z,Macias AR,Zerfas PM,Ward JM




Has Abstract


2008-01-04 00:00:00










  • How to engage Cofilin.

    abstract::In HIV-infected people, resting CD4+ T cells are the main reservoir of latent virus and the reason for the failure of drug therapy to cure HIV infection. Still, we do not have a complete understanding of the factors regulating HIV replication in these cells. A recent paper in Cell describes a new trick that the virus ...


    pub_type: 杂志文章,评审


    authors: Bukrinsky M

    更新日期:2008-09-22 00:00:00

  • Limelight on two HIV/SIV accessory proteins in macrophage infection: is Vpx overshadowing Vpr?

    abstract::HIV viruses encode a set of accessory proteins, which are important determinants of virulence due to their ability to manipulate the host cell physiology for the benefit of the virus. Although these viral proteins are dispensable for viral growth in many in vitro cell culture systems, they influence the efficiency of ...


    pub_type: 杂志文章,评审


    authors: Ayinde D,Maudet C,Transy C,Margottin-Goguet F

    更新日期:2010-04-09 00:00:00

  • Trypanosoma cruzi (Chagas' disease agent) reduces HIV-1 replication in human placenta.

    abstract:BACKGROUND:Several factors determine the risk of HIV mother-to-child transmission (MTCT), such as coinfections in placentas from HIV-1 positive mothers with other pathogens. Chagas' disease is one of the most endemic zoonoses in Latin America, caused by the protozoan Trypanosoma cruzi. The purpose of the study was to d...


    pub_type: 杂志文章


    authors: Dolcini GL,Solana ME,Andreani G,Celentano AM,Parodi LM,Donato AM,Elissondo N,González Cappa SM,Giavedoni LD,Martínez Peralta L

    更新日期:2008-07-01 00:00:00

  • Biochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase.

    abstract:BACKGROUND:The 18 residue tail abutting the SH3 fold that comprises the heart of the C-terminal domain is the only part of HIV-1 integrase yet to be visualized by structural biology. To ascertain the role of the tail region in integrase function and HIV-1 replication, a set of deletion mutants that successively lacked ...


    pub_type: 杂志文章


    authors: Dar MJ,Monel B,Krishnan L,Shun MC,Di Nunzio F,Helland DE,Engelman A

    更新日期:2009-10-19 00:00:00

  • Moving beyond the mousetrap: current and emerging humanized mouse and rat models for investigating prevention and cure strategies against HIV infection and associated pathologies.

    abstract::The development of safe and effective combination antiretroviral therapies for human immunodeficiency virus (HIV) infection over the past several decades has significantly reduced HIV-associated morbidity and mortality. Additionally, antiretroviral drugs have provided an effective means of protection against HIV trans...


    pub_type: 杂志文章,评审


    authors: Agarwal Y,Beatty C,Biradar S,Castronova I,Ho S,Melody K,Bility MT

    更新日期:2020-04-10 00:00:00

  • Memories of John N. Brady: scientist, mentor and friend.

    abstract::Friends and colleagues remember John N. Brady, Ph.D., Chief of the Virus Tumor Biology Section of the Laboratory of Cellular Oncology, who died much too young at the age of 57 on April 27, 2009 of colon cancer. John grew up in Illinois and received his Ph.D. with Dr. Richard Consigli at Kansas State University studyin...


    pub_type: 传,社论,历史文章


    authors: Pise-Masison CA,Marriott SJ

    更新日期:2009-05-19 00:00:00

  • Utilization of HIV-1 envelope V3 to identify X4- and R5-specific Tat and LTR sequence signatures.

    abstract:BACKGROUND:HIV-1 entry is a receptor-mediated process directed by the interaction of the viral envelope with the host cell CD4 molecule and one of two co-receptors, CCR5 or CXCR4. The amino acid sequence of the third variable (V3) loop of the HIV-1 envelope is highly predictive of co-receptor utilization preference dur...


    pub_type: 杂志文章


    authors: Antell GC,Dampier W,Aiamkitsumrit B,Nonnemacher MR,Jacobson JM,Pirrone V,Zhong W,Kercher K,Passic S,Williams JW,Schwartz G,Hershberg U,Krebs FC,Wigdahl B

    更新日期:2016-05-03 00:00:00

  • Evolution and gene capture in ancient endogenous retroviruses - insights from the crocodilian genomes.

    abstract:BACKGROUND:Crocodilians are thought to be hosts to a diverse and divergent complement of endogenous retroviruses (ERVs) but a comprehensive investigation is yet to be performed. The recent sequencing of three crocodilian genomes provides an opportunity for a more detailed and accurate representation of the ERV diversit...


    pub_type: 杂志文章


    authors: Chong AY,Kojima KK,Jurka J,Ray DA,Smit AF,Isberg SR,Gongora J

    更新日期:2014-12-12 00:00:00

  • Identification of small molecule modulators of HIV-1 Tat and Rev protein accumulation.

    abstract:BACKGROUND:HIV-1 replication is critically dependent upon controlled processing of its RNA and the activities provided by its encoded regulatory factors Tat and Rev. A screen of small molecule modulators of RNA processing identified several which inhibited virus gene expression, affecting both relative abundance of spe...


    pub_type: 杂志文章


    authors: Balachandran A,Wong R,Stoilov P,Pan S,Blencowe B,Cheung P,Harrigan PR,Cochrane A

    更新日期:2017-01-26 00:00:00

  • NHEJ pathway is involved in post-integrational DNA repair due to Ku70 binding to HIV-1 integrase.

    abstract:BACKGROUND:HIV-1 integration results in genomic DNA gaps that are repaired by cellular DNA repair pathways. This step of the lentiviral life cycle remains poorly understood despite its crucial importance for successful replication. We and others reported that Ku70 protein of the non-homologous end joining pathway (NHEJ...


    pub_type: 杂志文章


    authors: Knyazhanskaya E,Anisenko A,Shadrina O,Kalinina A,Zatsepin T,Zalevsky A,Mazurov D,Gottikh M

    更新日期:2019-11-06 00:00:00

  • Digital PCR as a tool to measure HIV persistence.

    abstract::Although antiretroviral therapy is able to suppress HIV replication in infected patients, the virus persists and rebounds when treatment is stopped. In order to find a cure that can eradicate the latent reservoir, one must be able to quantify the persisting virus. Traditionally, HIV persistence studies have used real-...


    pub_type: 杂志文章,评审


    authors: Rutsaert S,Bosman K,Trypsteen W,Nijhuis M,Vandekerckhove L

    更新日期:2018-01-30 00:00:00

  • Primate lentiviral Nef proteins deregulate T-cell development by multiple mechanisms.

    abstract:BACKGROUND:A nef gene is present in all primate lentiviral genomes and is important for high viral loads and progression to AIDS in human or experimental macaque hosts of HIV or SIV, respectively. In these hosts, infection of the thymus results in a decreased output of naive T cells that may contribute to the developme...


    pub_type: 杂志文章


    authors: Van Nuffel A,Ariën KK,Stove V,Schindler M,O'Neill E,Schmökel J,Van de Walle I,Naessens E,Vanderstraeten H,Van Landeghem K,Taghon T,Pulkkinen K,Saksela K,Garcia JV,Fackler OT,Kirchhoff F,Verhasselt B

    更新日期:2013-11-15 00:00:00

  • Preferences for the selection of unique tRNA primers revealed from analysis of HIV-1 replication in peripheral blood mononuclear cells.

    abstract:BACKGROUND:All human immunodeficiency virus (HIV-1) uses a host tRNALys,3 as the primer for reverse transcription. The tRNALys,3 is bound to a region on the HIV-1 genome, the primer-binding site (PBS), that is complementary to the 18 terminal nucleotides of tRNALys,3. How HIV-1 selects the tRNA from the intracellular m...


    pub_type: 杂志文章


    authors: Moore-Rigdon KL,Kosloff BR,Kirkman RL,Morrow CD

    更新日期:2005-03-24 00:00:00

  • Reservoir cells no longer detectable after a heterologous SHIV challenge with the synthetic HIV-1 Tat Oyi vaccine.

    abstract:BACKGROUND:Extra-cellular roles of Tat might be the main cause of maintenance of HIV-1 infected CD4 T cells or reservoir cells. We developed a synthetic vaccine based on a Tat variant of 101 residues called Tat Oyi, which was identified in HIV infected patients in Africa who did not progress to AIDS. We compared, using...


    pub_type: 杂志文章


    authors: Watkins JD,Lancelot S,Campbell GR,Esquieu D,de Mareuil J,Opi S,Annappa S,Salles JP,Loret EP

    更新日期:2006-01-27 00:00:00

  • The HIV-1 Rev/RRE system is required for HIV-1 5' UTR cis elements to augment encapsidation of heterologous RNA into HIV-1 viral particles.

    abstract:BACKGROUND:The process of HIV-1 genomic RNA (gRNA) encapsidation is governed by a number of viral encoded components, most notably the Gag protein and gRNA cis elements in the canonical packaging signal (ψ). Also implicated in encapsidation are cis determinants in the R, U5, and PBS (primer binding site) from the 5' un...


    pub_type: 杂志文章


    authors: Cockrell AS,van Praag H,Santistevan N,Ma H,Kafri T

    更新日期:2011-06-24 00:00:00

  • Efficient trapping of HIV-1 envelope protein by hetero-oligomerization with an N-helix chimera.

    abstract:BACKGROUND:The N-heptad repeat region of the HIV-1 Transmembrane Envelope protein is a trimerization domain that forms part of a "six helix bundle" crucial to Envelope-mediated membrane fusion. N-heptad repeat peptides have been used as extracellular reagents to inhibit virus fusion. RESULTS:When expressed intracellul...


    pub_type: 杂志文章


    authors: Ou W,Silver J

    更新日期:2005-08-10 00:00:00

  • Identification of a tripartite interaction between the N-terminus of HIV-1 Vif and CBFβ that is critical for Vif function.

    abstract:BACKGROUND:HIV-1 Vif interacts with the cellular core-binding factor β (CBFβ) and counteracts the protective roles of certain human APOBEC3 (A3) proteins by targeting them for proteasomal degradation. Previous studies have identified some amino acids important for Vif-CBFβ interactions, and recently a co-crystal struct...


    pub_type: 杂志文章


    authors: Desimmie BA,Smith JL,Matsuo H,Hu WS,Pathak VK

    更新日期:2017-03-17 00:00:00

  • DBR1 siRNA inhibition of HIV-1 replication.

    abstract:BACKGROUND:HIV-1 and all retroviruses are related to retroelements of simpler organisms such as the yeast Ty elements. Recent work has suggested that the yeast retroelement Ty1 replicates via an unexpected RNA lariat intermediate in cDNA synthesis. The putative genomic RNA lariat intermediate is formed by a 2'-5' phosp...


    pub_type: 杂志文章


    authors: Ye Y,De Leon J,Yokoyama N,Naidu Y,Camerini D

    更新日期:2005-10-18 00:00:00

  • Modulation of HIV-1-host interaction: role of the Vpu accessory protein.

    abstract::Viral protein U (Vpu) is a type 1 membrane-associated accessory protein that is unique to human immunodeficiency virus type 1 (HIV-1) and a subset of related simian immunodeficiency virus (SIV). The Vpu protein encoded by HIV-1 is associated with two primary functions during the viral life cycle. First, it contributes...


    pub_type: 杂志文章,评审


    authors: Dubé M,Bego MG,Paquay C,Cohen ÉA

    更新日期:2010-12-22 00:00:00

  • Discovery of a diaminoquinoxaline benzenesulfonamide antagonist of HIV-1 Nef function using a yeast-based phenotypic screen.

    abstract:BACKGROUND:HIV-1 Nef is a viral accessory protein critical for AIDS progression. Nef lacks intrinsic catalytic activity and binds multiple host cell signaling proteins, including Hck and other Src-family tyrosine kinases. Nef binding induces constitutive Hck activation that may contribute to HIV pathogenesis by promoti...


    pub_type: 杂志文章


    authors: Trible RP,Narute P,Emert-Sedlak LA,Alvarado JJ,Atkins K,Thomas L,Kodama T,Yanamala N,Korotchenko V,Day BW,Thomas G,Smithgall TE

    更新日期:2013-11-14 00:00:00

  • Caveolin-1 reduces HIV-1 infectivity by restoration of HIV Nef mediated impairment of cholesterol efflux by apoA-I.

    abstract:BACKGROUND:HIV infection results in inhibited cholesterol efflux by apolipoprotein A-I (apoA-I) in macrophages, and this impairment involves Nef mediated down-regulation and redistribution of ATP-binding cassette transporter A1 (ABCA-1). We investigated the effect of caveolin-1 (Cav-1) on the cholesterol efflux by apoA...


    pub_type: 杂志文章


    authors: Lin S,Nadeau PE,Wang X,Mergia A

    更新日期:2012-10-15 00:00:00

  • Low-level alternative tRNA priming of reverse transcription of HIV-1 and SIV in vivo.

    abstract:BACKGROUND:Reverse transcription (RT) of HIV and SIV is initiated by the binding of the acceptor stem of tRNALys3 to the primer binding site (PBS) of the viral RNA genome. Previous studies have suggested that this tRNALys3 is not the only molecule capable of priming reverse transcription, and that at least one other ly...


    pub_type: 杂志文章


    authors: Fennessey CM,Camus C,Immonen TT,Reid C,Maldarelli F,Lifson JD,Keele BF

    更新日期:2019-04-04 00:00:00

  • Induction of p21(CIP1/WAF1) expression by human T-lymphotropic virus type 1 Tax requires transcriptional activation and mRNA stabilization.

    abstract::HTLV-1 Tax can induce senescence by up-regulating the levels of cyclin-dependent kinase inhibitors p21(CIP1/WAF1) and p27(KIP1). Tax increases p27(KIP1) protein stability by activating the anaphase promoting complex/cyclosome (APC/C) precociously, causing degradation of Skp2 and inactivation of SCF(Skp2), the E3 ligas...


    pub_type: 杂志文章


    authors: Zhang L,Zhi H,Liu M,Kuo YL,Giam CZ

    更新日期:2009-04-08 00:00:00

  • Lipid levels, insulin resistance and cardiovascular risk over 96 weeks of antiretroviral therapy: a randomised controlled trial comparing low-dose stavudine and tenofovir.

    abstract:BACKGROUND:HIV infection and antiretroviral treatment are associated with changes in lipid levels, insulin resistance and risk of cardiovascular disease (CVD). We investigated these changes in the first 96 weeks of treatment with low-dose stavudine or tenofovir regimens. METHODS:This is a secondary analysis of a doubl...


    pub_type: 杂志文章,随机对照试验


    authors: Vos AG,Chersich MF,Klipstein-Grobusch K,Zuithoff P,Moorhouse MA,Lalla-Edward ST,Kambugu A,Kumarasamy N,Grobbee DE,Barth RE,Venter WD

    更新日期:2018-12-14 00:00:00

  • Inhibition of Tat activity by the HEXIM1 protein.

    abstract:BACKGROUND:The positive transcription elongation factor b (P-TEFb) composed by CDK9/CyclinT1 subunits is a dedicated co-factor of HIV transcriptional transactivator Tat protein. Transcription driven by the long terminal repeat (LTR) of HIV involves formation of a quaternary complex between P-TEFb, Tat and the TAR eleme...


    pub_type: 杂志文章


    authors: Fraldi A,Varrone F,Napolitano G,Michels AA,Majello B,Bensaude O,Lania L

    更新日期:2005-07-02 00:00:00

  • The co-receptor signaling model of HIV-1 pathogenesis in peripheral CD4 T cells.

    abstract::HIV-mediated CD4 depletion is the hallmark of AIDS and is the most reliable predictor of disease progression. While HIV replication is associated with CD4 depletion in general, plasma viremia by itself predicts the rate of CD4 loss only minimally in untreated patients. To resolve this paradox, I hypothesize the existe...


    pub_type: 社论


    authors: Wu Y

    更新日期:2009-05-01 00:00:00

  • HIV-1 envelope replication and α4β7 utilization among newly infected subjects and their corresponding heterosexual partners.

    abstract:BACKGROUND:Previous studies suggest that active selection limits the number of HIV-1 variants acquired by a newly infected individual from the diverse variants circulating in the transmitting partner. We compared HIV-1 envelopes from 9 newly infected subjects and their linked transmitting partner to explore potential m...


    pub_type: 杂志文章


    authors: Pena-Cruz V,Etemad B,Chatziandreou N,Nyein PH,Stock S,Reynolds SJ,Laeyendecker O,Gray RH,Serwadda D,Lee SJ,Quinn TC,Sagar M

    更新日期:2013-12-26 00:00:00

  • The HIV-1 integrase-LEDGF allosteric inhibitor MUT-A: resistance profile, impairment of virus maturation and infectivity but without influence on RNA packaging or virus immunoreactivity.

    abstract:BACKGROUND:HIV-1 Integrase (IN) interacts with the cellular co-factor LEDGF/p75 and tethers the HIV preintegration complex to the host genome enabling integration. Recently a new class of IN inhibitors was described, the IN-LEDGF allosteric inhibitors (INLAIs). Designed to interfere with the IN-LEDGF interaction during...


    pub_type: 杂志文章


    authors: Amadori C,van der Velden YU,Bonnard D,Orlov I,van Bel N,Le Rouzic E,Miralles L,Brias J,Chevreuil F,Spehner D,Chasset S,Ledoussal B,Mayr L,Moreau F,García F,Gatell J,Zamborlini A,Emiliani S,Ruff M,Klaholz BP,Moog C

    更新日期:2017-11-09 00:00:00

  • CD45 immunoaffinity depletion of vesicles from Jurkat T cells demonstrates that exosomes contain CD45: no evidence for a distinct exosome/HIV-1 budding pathway.

    abstract::The presence of relatively high levels of cellular protein contamination in density-purified virion preparations is a confounding factor in biochemical analyses of HIV and SIV produced from hematopoietic cells. A major source of this contamination is from vesicles, either microvesicles or exosomes, that have similar p...


    pub_type: 杂志文章


    authors: Coren LV,Shatzer T,Ott DE

    更新日期:2008-07-16 00:00:00

  • Natural APOBEC3C variants can elicit differential HIV-1 restriction activity.

    abstract:BACKGROUND:The APOBEC3 (A3) family of DNA cytosine deaminases provides an innate barrier to infection by retroviruses including HIV-1. A total of five enzymes, A3C, A3D, A3F, A3G and A3H, are degraded by the viral accessory protein Vif and expressed at high levels in CD4+ T cells, the primary reservoir for HIV-1 replic...


    pub_type: 杂志文章


    authors: Anderson BD,Ikeda T,Moghadasi SA,Martin AS,Brown WL,Harris RS

    更新日期:2018-12-17 00:00:00