Abstract:
:The mouse macrophage-like cell line RAW264.7, the most commonly used mouse macrophage cell line in medical research, was originally reported to be free of replication-competent murine leukemia virus (MuLV) despite its origin in a tumor induced by Abelson MuLV containing Moloney MuLV as helper virus. As currently available, however, we find that it produces significant levels of ecotropic MuLV with the biologic features of the Moloney isolate and also MuLV of the polytropic or MCF class. Newborn mice developed lymphoma following inoculation with the MuLV mixture expressed by these cells. These findings should be considered in interpretation of increasingly widespread use of these cells for propagation of other viruses, studies of biological responses to virus infection and use in RNA interference and cell signalling studies.
journal_name
Retrovirologyjournal_title
Retrovirologyauthors
Hartley JW,Evans LH,Green KY,Naghashfar Z,Macias AR,Zerfas PM,Ward JMdoi
10.1186/1742-4690-5-1subject
Has Abstractpub_date
2008-01-04 00:00:00pages
1issn
1742-4690pii
1742-4690-5-1journal_volume
5pub_type
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