Abstract:
BACKGROUND:HIV infection results in inhibited cholesterol efflux by apolipoprotein A-I (apoA-I) in macrophages, and this impairment involves Nef mediated down-regulation and redistribution of ATP-binding cassette transporter A1 (ABCA-1). We investigated the effect of caveolin-1 (Cav-1) on the cholesterol efflux by apoA-I in HIV infected primary and THP-1 cell-differentiated macrophages as well as astrocyte derived glioblastoma U87 cells. RESULTS:Our results reveal that Cav-1 restores the Nef -mediated impairment of cholesterol efflux by apoA-I in both cell types. Co-immunoprecipitation studies indicate a physical association of Cav-1 and Nef. The level of ABCA-1 expression remains the same whether Cav-1 is over-expressed or not. In addition, we examined the cholesterol composition of HIV particles released from Cav-1 treated cells and identified that the cholesterol content is dramatically reduced. The infectivity level of these virus particles is also significantly decreased. CONCLUSIONS:These observations suggest that the interplay of Cav-1 with Nef and cholesterol subsequently counters Nef induced impairment of cholesterol efflux by apoA-l. The findings provide a cellular mechanism by which Cav-1 has an ability to restore HIV mediated impairment of cholesterol efflux in macrophages. This subsequently influences the cholesterol content incorporated into virus particles thereby inhibiting HIV infectivity and contributing to HIV's persistent infection of macrophages.
journal_name
Retrovirologyjournal_title
Retrovirologyauthors
Lin S,Nadeau PE,Wang X,Mergia Adoi
10.1186/1742-4690-9-85subject
Has Abstractpub_date
2012-10-15 00:00:00pages
85issn
1742-4690pii
1742-4690-9-85journal_volume
9pub_type
杂志文章相关文献
Retrovirology文献大全abstract:BACKGROUND:MicroRNA (miRNA)-mediated RNA silencing is integral to virtually every cellular process including cell cycle progression and response to virus infection. The interplay between RNA silencing and HIV-1 is multifaceted, and accumulating evidence posits a strike-counterstrike interface that alters the cellular e...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/1742-4690-8-36
更新日期:2011-05-13 00:00:00
abstract:BACKGROUND:Extra-cellular roles of Tat might be the main cause of maintenance of HIV-1 infected CD4 T cells or reservoir cells. We developed a synthetic vaccine based on a Tat variant of 101 residues called Tat Oyi, which was identified in HIV infected patients in Africa who did not progress to AIDS. We compared, using...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/1742-4690-3-8
更新日期:2006-01-27 00:00:00
abstract:BACKGROUND:Adult T-cell leukemia lymphoma (ATLL) is a chemotherapy-resistant malignancy with a median survival of less than one year that will afflict between one hundred thousand and one million individuals worldwide who are currently infected with human T-cell leukemia virus type 1. Recurrent somatic mutations in hos...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/s12977-020-00535-z
更新日期:2020-08-28 00:00:00
abstract::This editorial represents a plea to retrovirologists to attend the XVI International Conference on AIDS that will take place in Toronto, Canada between August 13-18, 2006. In short, it is vital that politicians and opinion leaders understand that basic scientists are no less committed to the worldwide battle against H...
journal_title:Retrovirology
pub_type: 社论
doi:10.1186/1742-4690-3-9
更新日期:2006-02-03 00:00:00
abstract::Friends and colleagues remember John N. Brady, Ph.D., Chief of the Virus Tumor Biology Section of the Laboratory of Cellular Oncology, who died much too young at the age of 57 on April 27, 2009 of colon cancer. John grew up in Illinois and received his Ph.D. with Dr. Richard Consigli at Kansas State University studyin...
journal_title:Retrovirology
pub_type: 传,社论,历史文章
doi:10.1186/1742-4690-6-48
更新日期:2009-05-19 00:00:00
abstract::Correlates of protection (CoPs) against infection by primate lentiviruses remain undefined. Modest protection against HIV-1 was observed in one human vaccine trial, whereas previous trials and vaccine-challenge experiments in non-human primates have yielded inconsistent but intriguing results. Although high levels of ...
journal_title:Retrovirology
pub_type: 杂志文章,评审
doi:10.1186/1742-4690-9-80
更新日期:2012-10-01 00:00:00
abstract:BACKGROUND:The foamy viral genome encodes four central purine-rich elements localized in the integrase-coding region of pol. Previously, we have shown that the first two of these RNA elements (A and B) are required for protease dimerization and activation. The D element functions as internal polypurine tract during rev...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/s12977-017-0337-6
更新日期:2017-02-06 00:00:00
abstract:BACKGROUND:The Tax proteins encoded by human T lymphotropic virus type 1 (HTLV-1) and type 2 (HTLV-2) are transcriptional activators of both the viral long terminal repeat (LTR) and cellular promoters via the CREB and NFkB pathways. In contrast to HTLV-1, HTLV-2 has been classified into four distinct genetic subtypes A...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/1742-4690-3-20
更新日期:2006-03-21 00:00:00
abstract:BACKGROUND:We have previously reported that CD4 T cells from some exposed uninfected (EU) Vietnamese intravenous drug users are relatively resistant to HIV infection in vitro. Here, we further characterized the restriction of viral replication in CD4 T cells from five EUs and assessed its persistence in serial samples....
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/1742-4690-3-81
更新日期:2006-11-08 00:00:00
abstract:BACKGROUND:Despite intensive investigation the mechanism by which HIV-1 reaches the host cell nucleus is unknown. TNPO3, a karyopherin mediating nuclear entry of SR-proteins, was shown to be required for HIV-1 infectivity. Some investigators have reported that TNPO3 promotes HIV-1 nuclear import, as would be expected f...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/1742-4690-10-20
更新日期:2013-02-15 00:00:00
abstract:BACKGROUND:HIV-1 capsid influences viral uncoating and nuclear import. Some capsid is detected in the nucleus but it is unclear if it has any function. We reported that the antibiotic Coumermycin-A1 (C-A1) inhibits HIV-1 integration and that a capsid mutation confers resistance to C-A1, suggesting that capsid might aff...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/s12977-016-0262-0
更新日期:2016-04-23 00:00:00
abstract::Sidaction's appeal regarding the sentencing of medical personnels in the Libyan-HIV infection cases. ...
journal_title:Retrovirology
pub_type: 社论
doi:10.1186/1742-4690-3-98
更新日期:2006-12-27 00:00:00
abstract:BACKGROUND:The N-heptad repeat region of the HIV-1 Transmembrane Envelope protein is a trimerization domain that forms part of a "six helix bundle" crucial to Envelope-mediated membrane fusion. N-heptad repeat peptides have been used as extracellular reagents to inhibit virus fusion. RESULTS:When expressed intracellul...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/1742-4690-2-51
更新日期:2005-08-10 00:00:00
abstract:BACKGROUND:In the acute phase of infection with feline immunodeficiency virus (FIV), the virus targets activated CD4+ T cells by utilising CD134 (OX40) as a primary attachment receptor and CXCR4 as a co-receptor. The nature of the virus-receptor interaction varies between isolates; strains such as GL8 and CPGammer reco...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/1742-4690-7-38
更新日期:2010-04-26 00:00:00
abstract:BACKGROUND:HIV-1 Vif interacts with the cellular core-binding factor β (CBFβ) and counteracts the protective roles of certain human APOBEC3 (A3) proteins by targeting them for proteasomal degradation. Previous studies have identified some amino acids important for Vif-CBFβ interactions, and recently a co-crystal struct...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/s12977-017-0346-5
更新日期:2017-03-17 00:00:00
abstract::In the mid-1990's, researchers hypothesized, based on new viral load data, that HIV-1 causes CD4+ T-cell depletion by direct cytopathic effect. New data from non-human primate studies has raised doubts about this model of HIV-1 pathogenesis. Despite having high levels of viremia, most SIV infections are well tolerated...
journal_title:Retrovirology
pub_type: 社论
doi:10.1186/1742-4690-3-60
更新日期:2006-09-08 00:00:00
abstract::The identification of the most appropriate marker to measure reservoir size has been a great challenge for the HIV field. Quantitative viral outgrowth assay (QVOA), the reference standard to quantify the amount of replication-competent virus, has several limitations, as it is laborious, expensive, and unable to robust...
journal_title:Retrovirology
pub_type: 杂志文章,评审
doi:10.1186/s12977-018-0396-3
更新日期:2018-02-17 00:00:00
abstract:BACKGROUND:The 18 residue tail abutting the SH3 fold that comprises the heart of the C-terminal domain is the only part of HIV-1 integrase yet to be visualized by structural biology. To ascertain the role of the tail region in integrase function and HIV-1 replication, a set of deletion mutants that successively lacked ...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/1742-4690-6-94
更新日期:2009-10-19 00:00:00
abstract:BACKGROUND:HIV-1 Nef is a viral accessory protein critical for AIDS progression. Nef lacks intrinsic catalytic activity and binds multiple host cell signaling proteins, including Hck and other Src-family tyrosine kinases. Nef binding induces constitutive Hck activation that may contribute to HIV pathogenesis by promoti...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/1742-4690-10-135
更新日期:2013-11-14 00:00:00
abstract::Susceptibility to HIV-1 and the clinical course after infection show a substantial heterogeneity between individuals. Part of this variability can be attributed to host genetic variation. Initial candidate gene studies have revealed interesting host factors that influence HIV infection, replication and pathogenesis. R...
journal_title:Retrovirology
pub_type: 杂志文章,评审
doi:10.1186/1742-4690-9-70
更新日期:2012-08-24 00:00:00
abstract::An amendment to this paper has been published and can be accessed via the original article. ...
journal_title:Retrovirology
pub_type: 杂志文章,已发布勘误
doi:10.1186/s12977-020-00531-3
更新日期:2020-07-29 00:00:00
abstract:BACKGROUND:The human T-cell leukemia virus type 1 (HTLV-1) Tax protein indirectly influences transcriptional activation, signal transduction, cell cycle control, and apoptosis. The function of Tax primarily relies on protein-protein interactions. We have previously shown that Tax upregulates the cell cycle checkpoint p...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/1742-4690-1-6
更新日期:2004-04-13 00:00:00
abstract:BACKGROUND:The continued persistence of HIV-1 as a public health concern due to the lack of a cure calls for the development of new tools for studying replication of the virus. Here, we used NanoLuc, a small and extremely bright luciferase protein, to develop an HIV-1 bioluminescent reporter virus that simplifies funct...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/s12977-020-00521-5
更新日期:2020-05-19 00:00:00
abstract:BACKGROUND:Protein arginine methyltransferase 6 (PRMT6) is a nuclear enzyme that methylates arginine residues on histones and transcription factors. In addition, PRMT6 inhibits HIV-1 replication in cell culture by directly methylating and interfering with the functions of several HIV-1 proteins, i.e. Tat, Rev and nucle...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/1742-4690-10-73
更新日期:2013-07-17 00:00:00
abstract:BACKGROUND:The positive transcription elongation factor b (P-TEFb) composed by CDK9/CyclinT1 subunits is a dedicated co-factor of HIV transcriptional transactivator Tat protein. Transcription driven by the long terminal repeat (LTR) of HIV involves formation of a quaternary complex between P-TEFb, Tat and the TAR eleme...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/1742-4690-2-42
更新日期:2005-07-02 00:00:00
abstract:BACKGROUND:Host SAM domain and HD domain-containing protein 1 (SAMHD1) suppresses reverse transcription kinetics of HIV-1 in nondividing cells such as macrophages by hydrolyzing and nearly depleting cellular dNTPs, which are the substrates of viral reverse transcriptase (RT). However, unlike HIV-1, HIV-2 and SIVsm enco...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/s12977-014-0111-y
更新日期:2014-12-19 00:00:00
abstract:BACKGROUND:Human T-cell lymphotropic virus type 1 (HTLV-1) is the etiological agent of HTLV associated myelopathy/ Tropical Spastic Paraparesis (HAM/TSP) and Adult T cell leukemia/lymphoma (ATLL), in around 2-5% of the infected individuals. Host genetic background might play a role in disease progression. Several previ...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/s12977-020-00536-y
更新日期:2020-09-03 00:00:00
abstract::In HIV-infected people, resting CD4+ T cells are the main reservoir of latent virus and the reason for the failure of drug therapy to cure HIV infection. Still, we do not have a complete understanding of the factors regulating HIV replication in these cells. A recent paper in Cell describes a new trick that the virus ...
journal_title:Retrovirology
pub_type: 杂志文章,评审
doi:10.1186/1742-4690-5-85
更新日期:2008-09-22 00:00:00
abstract:BACKGROUND:Unlike CD4+ T cells, HIV-1 infected macrophages exhibit extended life span even upon stress, consistent with their in vivo role as long-lived HIV-1 reservoirs. RESULTS:Here, we demonstrate that PI3K/Akt inhibitors, including clinically available Miltefosine, dramatically reduced HIV-1 production from long-l...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/1742-4690-5-11
更新日期:2008-01-31 00:00:00
abstract::Efforts to assess the prevalence of xenotropic murine leukemia virus-related virus (XMRV) in patients with prostate cancer and chronic fatigue syndrome have relied heavily on PCR-based testing of clinical samples and have yielded widely divergent findings. This week in Retrovirology, reports from four independent rese...
journal_title:Retrovirology
pub_type: 杂志文章
doi:10.1186/1742-4690-7-112
更新日期:2010-12-20 00:00:00