Contamination of clinical specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retroviruses.

Abstract:

:Efforts to assess the prevalence of xenotropic murine leukemia virus-related virus (XMRV) in patients with prostate cancer and chronic fatigue syndrome have relied heavily on PCR-based testing of clinical samples and have yielded widely divergent findings. This week in Retrovirology, reports from four independent research groups illustrate the extreme care needed to exclude DNA or RNA contamination in PCR analyses of XMRV. In addition, phylogenetic evidence suggesting that previously-published XMRV sequences originated from a commonly-used prostate carcinoma cell line (22Rv1) is presented. These findings raise important questions regarding the provenance of XMRV and its potential connection to human disease.

journal_name

Retrovirology

journal_title

Retrovirology

authors

Smith RA

doi

10.1186/1742-4690-7-112

subject

Has Abstract

pub_date

2010-12-20 00:00:00

pages

112

issn

1742-4690

pii

1742-4690-7-112

journal_volume

7

pub_type

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