Abstract：
:We have obtained the 1.7 A crystal structure of FIV protease (PR) in which 12 critical residues around the active site have been substituted with the structurally equivalent residues of HIV PR (12X FIV PR). The chimeric PR was crystallized in complex with the broad-based inhibitor TL-3, which inhibits wild type FIV and HIV PRs, as well as 12X FIV PR and several drug-resistant HIV mutants 1234. Biochemical analyses have demonstrated that TL-3 inhibits these PRs in the order HIV PR > 12X FIV PR > FIV PR, with Ki values of 1.5 nM, 10 nM, and 41 nM, respectively 234. Comparison of the crystal structures of the TL-3 complexes of 12X FIV and wild-typeFIV PR revealed theformation of additinal van der Waals interactions between the enzyme inhibitor in the mutant PR. The 12X FIV PR retained the hydrogen bonding interactions between residues in the flap regions and active site involving the enzyme and the TL-3 inhibitor in comparison to both FIV PR and HIV PR. However, the flap regions of the 12X FIV PR more closely resemble those of HIV PR, having gained several stabilizing intra-flap interactions not present in wild type FIV PR. These findings offer a structural explanation for the observed inhibitor/substrate binding properties of the chimeric PR.
journal_name
Retrovirologyjournal_title
Retrovirologyauthors
Heaslet H,Lin YC,Tam K,Torbett BE,Elder JH,Stout CDdoi
10.1186/1742-4690-4-1subject
Has Abstractpub_date
2007-01-09 00:00:00pages
1issn
1742-4690pii
1742-4690-4-1journal_volume
4pub_type
杂志文章相关文献
Retrovirology文献大全abstract：:Recent findings suggest that mammalian cells can use small non-coding RNAs (ncRNA) to regulate physiological viral infections. Here, we comment on several lines of evidence that support this concept. We discuss how viruses may in turn protect, suppress, evade, modulate, or adapt to the host cell's ncRNA regulatory sch...
journal_title：Retrovirology
pub_type： 社论,评审
doi：10.1186/1742-4690-4-74
更新日期：2007-10-15 00:00:00
abstract：BACKGROUND:HIV-1 budding is directed primarily by two motifs in Gag p6 designated as late domain-1 and -2 that recruit ESCRT machinery by binding Tsg101 and Alix, respectively, and by poorly characterized determinants in the capsid (CA) domain. Here, we report that a conserved Gag p6 residue, S40, impacts budding media...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-10-143
更新日期：2013-11-21 00:00:00
abstract：BACKGROUND:The HIV-1 Rev regulatory protein binds as an oligomeric complex to viral RNA mediating nuclear export of incompletely spliced and non-spliced viral mRNAs encoding the viral structural proteins. However, the biological significance of the obligatory complex formation of Rev upon the viral RNA is unclear. RES...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-2-39
更新日期：2005-06-10 00:00:00
abstract：BACKGROUND:The development of anaemia in feline leukaemia virus (FeLV)-infected cats is associated with the emergence of a novel viral subgroup, FeLV-C. FeLV-C arises from the subgroup that is transmitted, FeLV-A, through alterations in the amino acid sequence of the receptor binding domain (RBD) of the envelope glycop...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-9-48
更新日期：2012-05-31 00:00:00
abstract：:The King's College London (KCL) Infectious Diseases BioBank opened in 2007 and collects peripheral venous blood (PVB) from individuals infected with pathogens including human immunodeficiency virus (HIV). PVBs are fractionated into plasmas, lymphocytes and DNA and are then frozen. All donations are from subjects who h...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-6-98
更新日期：2009-11-03 00:00:00
abstract：BACKGROUND:We have previously reported that CD4 T cells from some exposed uninfected (EU) Vietnamese intravenous drug users are relatively resistant to HIV infection in vitro. Here, we further characterized the restriction of viral replication in CD4 T cells from five EUs and assessed its persistence in serial samples....
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-3-81
更新日期：2006-11-08 00:00:00
abstract：:In HIV-infected people, resting CD4+ T cells are the main reservoir of latent virus and the reason for the failure of drug therapy to cure HIV infection. Still, we do not have a complete understanding of the factors regulating HIV replication in these cells. A recent paper in Cell describes a new trick that the virus ...
journal_title：Retrovirology
pub_type： 杂志文章,评审
doi：10.1186/1742-4690-5-85
更新日期：2008-09-22 00:00:00
abstract：:As HIV has fueled a global resurgence of tuberculosis over the last several decades, there is a growing awareness that HIV-mediated impairments in both innate and adaptive immunity contribute to the heightened risk of tuberculosis in people with HIV. Since early immune responses to Mycobacterium tuberculosis (Mtb) set...
journal_title：Retrovirology
pub_type： 杂志文章,评审
doi：10.1186/s12977-020-00540-2
更新日期：2020-09-23 00:00:00
abstract：:Viral protein U (Vpu) is a type 1 membrane-associated accessory protein that is unique to human immunodeficiency virus type 1 (HIV-1) and a subset of related simian immunodeficiency virus (SIV). The Vpu protein encoded by HIV-1 is associated with two primary functions during the viral life cycle. First, it contributes...
journal_title：Retrovirology
pub_type： 杂志文章,评审
doi：10.1186/1742-4690-7-114
更新日期：2010-12-22 00:00:00
abstract：BACKGROUND:Recognition of microbial products through Toll-like receptors (TLRs) initiates inflammatory responses orchestrated by innate immune cells such as dendritic cells (DCs). As these cells are patrolling mucosal surfaces, a portal of entry for various pathogens including human immunodeficiency virus type-1 (HIV-1...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-6-42
更新日期：2009-05-06 00:00:00
abstract：BACKGROUND:We and others have recently demonstrated that the human retrovirus HTLV-I was producing a spliced antisense transcript, which led to the synthesis of the HBZ protein. The objective of the present study was to demonstrate the existence of antisense transcription in HIV-1 and to provide a better characterizati...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-4-71
更新日期：2007-10-02 00:00:00
abstract：:Sidaction's appeal regarding the sentencing of medical personnels in the Libyan-HIV infection cases. ...
journal_title：Retrovirology
pub_type： 社论
doi：10.1186/1742-4690-3-98
更新日期：2006-12-27 00:00:00
abstract：BACKGROUND:Xenotropic murine leukemia virus (MLV)-related virus (XMRV) was initially identified in prostate cancer (PCa) tissue, particularly in the prostatic stromal fibroblasts, of patients homozygous for the RNASEL R462Q mutation. A subsequent study reported XMRV antigens in malignant prostatic epithelium and associ...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-8-23
更新日期：2011-03-29 00:00:00
abstract：BACKGROUND:HIV-1 infects a wide range of CD4+ T cells with different phenotypic properties and differing expression levels of entry coreceptors. We sought to determine the viral tropism of subtype C (C-HIV) Envelope (Env) clones for different CD4+ T cell subsets and whether tropism changes during acute to chronic disea...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/s12977-020-00532-2
更新日期：2020-08-06 00:00:00
abstract：BACKGROUND:A critical step for retroviral replication is the stable integration of the provirus into the genome of its host. The viral integrase protein is key in this essential step of the retroviral life cycle. Although the basic mechanism of integration by mammalian retroviruses has been well characterized, the fact...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-5-48
更新日期：2008-06-13 00:00:00
abstract：BACKGROUND:Several factors determine the risk of HIV mother-to-child transmission (MTCT), such as coinfections in placentas from HIV-1 positive mothers with other pathogens. Chagas' disease is one of the most endemic zoonoses in Latin America, caused by the protozoan Trypanosoma cruzi. The purpose of the study was to d...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-5-53
更新日期：2008-07-01 00:00:00
abstract：BACKGROUND:Among human T cell leukemia virus type 1 (HTLV-1)-infected individuals, there is an association between HTLV-1 tax subgroups (subgroup-A or subgroup-B) and the risk of HAM/TSP in the Japanese population. To investigate the role of HTLV-1 subgroups in viral pathogenesis, we studied the functional difference i...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/s12977-018-0454-x
更新日期：2018-11-06 00:00:00
abstract：BACKGROUND:Mutations in the substrate of HIV-1 protease, especially changes in the NC/p1 cleavage site, can directly contribute to protease inhibitor (PI) resistance and also compensate for defects in viral replicative capacity (RC) due to a drug resistant protease. These NC/p1 changes are known to enhance processing o...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-9-29
更新日期：2012-04-01 00:00:00
abstract：:Susceptibility to HIV-1 and the clinical course after infection show a substantial heterogeneity between individuals. Part of this variability can be attributed to host genetic variation. Initial candidate gene studies have revealed interesting host factors that influence HIV infection, replication and pathogenesis. R...
journal_title：Retrovirology
pub_type： 杂志文章,评审
doi：10.1186/1742-4690-9-70
更新日期：2012-08-24 00:00:00
abstract：BACKGROUND:Interferon induced transmembrane proteins 1, 2 and 3 (IFITMs) belong to a family of highly related antiviral factors that have been shown to interfere with a large spectrum of viruses including Filoviruses, Coronaviruses, Influenza virus, Dengue virus and HIV-1. In all these cases, the reported mechanism of ...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/s12977-014-0103-y
更新日期：2014-11-25 00:00:00
abstract：BACKGROUND:Combination anti-viral therapies have reduced treatment failure rates by requiring multiple specific mutations to be selected on the same viral genome to impart high-level drug resistance. To determine if the common protease inhibitor resistance mutation L90M is only selected once or repeatedly on different ...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-5-7
更新日期：2008-01-25 00:00:00
abstract：BACKGROUND:Human T-cell lymphotropic virus type 1 (HTLV-1) is the etiological agent of HTLV associated myelopathy/ Tropical Spastic Paraparesis (HAM/TSP) and Adult T cell leukemia/lymphoma (ATLL), in around 2-5% of the infected individuals. Host genetic background might play a role in disease progression. Several previ...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/s12977-020-00536-y
更新日期：2020-09-03 00:00:00
abstract：:How best to evaluate scientists within a peer group is a difficult task. This editorial discusses the use of the H-index and total citations. It also raises the consideration of a mentoring-index and the value of understanding the frequency that a published paper is accessed by readers. ...
journal_title：Retrovirology
pub_type： 社论
doi：10.1186/1742-4690-5-106
更新日期：2008-11-25 00:00:00
abstract：BACKGROUND:The 18 residue tail abutting the SH3 fold that comprises the heart of the C-terminal domain is the only part of HIV-1 integrase yet to be visualized by structural biology. To ascertain the role of the tail region in integrase function and HIV-1 replication, a set of deletion mutants that successively lacked ...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-6-94
更新日期：2009-10-19 00:00:00
abstract：BACKGROUND:Early HIV-1 infection causes massive CD4+ T cell death in the gut and translocation of bacteria into the circulation. However, the programmed cell death (PCD) pathways used by HIV-1 to kill CD4+ T cells in the gut, and the impact of microbial exposure on T cell loss, remain unclear. Understanding mucosal HIV...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-11-14
更新日期：2014-02-04 00:00:00
abstract：:Members of the APOBEC family of cellular cytidine deaminases represent a recently identified group of proteins that provide immunity to infection by retroviruses and protect the cell from endogenous mobile retroelements. Yet, HIV-1 is largely immune to the intrinsic antiviral effects of APOBEC proteins because it enco...
journal_title：Retrovirology
pub_type： 杂志文章,评审
doi：10.1186/1742-4690-5-51
更新日期：2008-06-24 00:00:00
abstract：:Integration of the reverse transcribed viral genome into host chromatin is the hallmark of retroviral replication. Yet, during natural HIV infection, various unintegrated viral DNA forms exist in abundance. Though linear viral cDNA is the precursor to an integrated provirus, increasing evidence suggests that transcrip...
journal_title：Retrovirology
pub_type： 杂志文章,评审
doi：10.1186/1742-4690-8-52
更新日期：2011-07-01 00:00:00
abstract：BACKGROUND:While viruses have long been shown to capitalize on their limited genomic size by utilizing both strands of DNA or complementary DNA/RNA intermediates to code for viral proteins, it has been assumed that human retroviruses have all their major proteins translated only from the plus or sense strand of RNA, de...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-3-80
更新日期：2006-11-08 00:00:00
abstract：BACKGROUND:The positive transcription elongation factor b (P-TEFb) composed by CDK9/CyclinT1 subunits is a dedicated co-factor of HIV transcriptional transactivator Tat protein. Transcription driven by the long terminal repeat (LTR) of HIV involves formation of a quaternary complex between P-TEFb, Tat and the TAR eleme...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-2-42
更新日期：2005-07-02 00:00:00
abstract：BACKGROUND:MicroRNA (miRNA)-mediated RNA silencing is integral to virtually every cellular process including cell cycle progression and response to virus infection. The interplay between RNA silencing and HIV-1 is multifaceted, and accumulating evidence posits a strike-counterstrike interface that alters the cellular e...
journal_title：Retrovirology
pub_type： 杂志文章
doi：10.1186/1742-4690-8-36
更新日期：2011-05-13 00:00:00
