Selection of recombinant MVA by rescue of the essential D4R gene.

Abstract:

:Modified vaccinia virus Ankara (MVA) has become a promising vaccine vector due to its immunogenicity and its proven safety in humans. As a general approach for stringent and rapid selection of recombinant MVA, we assessed marker rescue of the essential viral D4R gene in an engineered deletion mutant that is fully replication defective in wild-type cells. Recombinant, replicating virus was obtained by re-introduction of the deleted viral gene as a dominant selection marker into the deletion mutant.

journal_name

Virol J

journal_title

Virology journal

authors

Ricci PS,Schäfer B,Kreil TR,Falkner FG,Holzer GW

doi

10.1186/1743-422X-8-529

subject

Has Abstract

pub_date

2011-12-12 00:00:00

pages

529

issn

1743-422X

pii

1743-422X-8-529

journal_volume

8

pub_type

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