Abstract:
:Modified vaccinia virus Ankara (MVA) has become a promising vaccine vector due to its immunogenicity and its proven safety in humans. As a general approach for stringent and rapid selection of recombinant MVA, we assessed marker rescue of the essential viral D4R gene in an engineered deletion mutant that is fully replication defective in wild-type cells. Recombinant, replicating virus was obtained by re-introduction of the deleted viral gene as a dominant selection marker into the deletion mutant.
journal_name
Virol Jjournal_title
Virology journalauthors
Ricci PS,Schäfer B,Kreil TR,Falkner FG,Holzer GWdoi
10.1186/1743-422X-8-529subject
Has Abstractpub_date
2011-12-12 00:00:00pages
529issn
1743-422Xpii
1743-422X-8-529journal_volume
8pub_type
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