Site directed biotinylation of filamentous phage structural proteins.

Abstract:

:Filamentous bacteriophages have been used in numerous applications for the display of antibodies and random peptide libraries. Here we describe the introduction of a 13 amino acid sequence LASIFEAQKIEWR (designated BT, which is biotinylated in vivo by E. coli) into the N termini of four of the five structural proteins of the filamentous bacteriophage fd (Proteins 3, 7, 8 and 9). The in vivo and in vitro biotinylation of the various phages were compared. The production of multifunctional phages and their application as affinity reagents are demonstrated.

journal_name

Virol J

journal_title

Virology journal

authors

Smelyanski L,Gershoni JM

doi

10.1186/1743-422X-8-495

subject

Has Abstract

pub_date

2011-11-01 00:00:00

pages

495

issn

1743-422X

pii

1743-422X-8-495

journal_volume

8

pub_type

杂志文章
  • Toll-like receptors genes polymorphisms and the occurrence of HCMV infection among pregnant women.

    abstract:BACKGROUND:Human cytomegalovirus (HCMV) is the most common cause of intrauterine infections worldwide. The toll-like receptors (TLRs) have been reported as important factors in immune response against HCMV. Particularly, TLR2, TLR4 and TLR9 have been shown to be involved in antiviral immunity. Evaluation of the role of...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-017-0730-8

    authors: Wujcicka W,Paradowska E,Studzińska M,Wilczyński J,Nowakowska D

    更新日期:2017-03-24 00:00:00

  • Vomiting and wasting disease associated with hemagglutinating encephalomyelitis viruses infection in piglets in Jilin, China.

    abstract::One coronavirus strain was isolated from brain tissues of ten piglets with evident clinical manifestations of vomiting, diarrhea and dyskinesia in Jilin province in China. Antigenic and genomic characterizations of the virus (isolate PHEV-JLsp09) were based on multiplex PCR and negative staining electron microscopy an...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-130

    authors: Gao W,Zhao K,Zhao C,Du C,Ren W,Song D,Lu H,Chen K,Li Z,Lan Y,Xie S,He W,Gao F

    更新日期:2011-03-21 00:00:00

  • Author Correction: In vitro virucidal activity of Echinaforce®, an Echinacea purpurea preparation, against coronaviruses, including common cold coronavirus 229E and SARS-CoV-2.

    abstract::The original version of the acknowledgement unfortunately contained a mistake. ...

    journal_title:Virology journal

    pub_type: 已发布勘误

    doi:10.1186/s12985-020-01439-2

    authors: Signer J,Jonsdottir HR,Albrich WC,Strasser M,Züst R,Ryter S,Ackermann-Gäumann R,Lenz N,Siegrist D,Suter A,Schoop R,Engler OB

    更新日期:2020-11-09 00:00:00

  • The transcription map of HPV11 in U2OS cells adequately reflects the initial and stable replication phases of the viral genome.

    abstract:BACKGROUND:Although prophylactic vaccines have been developed against HPV6, HPV11, HPV16 and HPV18 there is the clear unmet medical need in order to justify the development of drugs targeting human papillomavirus replication. The native host cells of HPVs are human primary keratinocytes which can be cultivated in raft ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-015-0292-6

    authors: Isok-Paas H,Männik A,Ustav E,Ustav M

    更新日期:2015-04-14 00:00:00

  • Molecular epidemiology of Newcastle disease virus isolates from vaccinated commercial poultry farms in non-epidemic areas of Japan.

    abstract:BACKGROUND:Newcastle Disease (ND) is a highly contagious and economically devastating disease of poultry. At present, limited molecular epidemiological data are available regarding the causes of ND outbreaks in vaccinated commercial poultry farms. Knowing the genomic characteristics of Newcastle disease virus (NDV) inf...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-10-330

    authors: Umali DV,Ito H,Suzuki T,Shirota K,Katoh H,Ito T

    更新日期:2013-11-09 00:00:00

  • The relationship between human leukocyte antigen-DP/DQ gene polymorphisms and the outcomes of HCV infection in a Chinese population.

    abstract:BACKGROUND:Recently, human leukocyte antigen (HLA) class-II gene polymorphisms have been reported to be related to Hepatitis C virus (HCV) infection and chronicity. The objective of this study was to explore the relationship of HLA-DP rs9277535 and HLA-DQ rs7453920 with the outcomes of HCV infection. METHODS:The rs927...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-017-0901-7

    authors: Huang P,Fan H,Tian T,Liao P,Li J,Yu R,Xia X,Feng Y,Wang J,Liu Y,Zhang Y,Yue M

    更新日期:2017-12-06 00:00:00

  • First construction of infectious clone for newly emerging mutation porcine circovirus type 2 (PCV2) followed by comparison with PCV2a and PCV2b genotypes in biological characteristics in vitro.

    abstract:BACKGROUND:Porcine circovirus type 2 (PCV2), the causative agent of postweaning multisystemic wasting syndrome (PMWS), is a serious economic problem in the swine industry. Different genotypes (PCV2a, PCV2b and PCV2d) of the virus are present in the clinical cases in China, and it is necessary to elucidate the pathogeni...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-291

    authors: Guo LJ,Lu YH,Huang LP,Wei YW,Wu HL,Liu CM

    更新日期:2011-06-10 00:00:00

  • Oncolytic activity of a coxsackievirus B3 strain in human endometrial cancer cell lines.

    abstract:BACKGROUND:Endometrial cancer (EC) is one of the most common gynecological malignancies globally. Although progress has been made in surgical and other adjuvant therapies, there is still a great need to develop new approaches to further reduce the incidence and mortality of EC. Oncolytic virotherapy offers a novel prom...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-018-0975-x

    authors: Lin Y,Wang W,Wan J,Yang Y,Fu W,Pan D,Cai L,Cheng T,Huang X,Wang Y

    更新日期:2018-04-10 00:00:00

  • Reduction of the survival time of pig xenotransplants by porcine cytomegalovirus.

    abstract:BACKGROUND:Xenotransplantation using pig cells, tissues and organs may help to overcome the shortage of human tissues and organs for the treatment of tissue and organ failure. Progress in the prevention of immunological rejection using genetically modified pigs and new, more effective, immunosuppression regimens will a...

    journal_title:Virology journal

    pub_type: 杂志文章,评审

    doi:10.1186/s12985-018-1088-2

    authors: Denner J

    更新日期:2018-11-08 00:00:00

  • Characterization of a non-pathogenic H5N1 influenza virus isolated from a migratory duck flying from Siberia in Hokkaido, Japan, in October 2009.

    abstract:BACKGROUND:Infection with H5N1 highly pathogenic avian influenza viruses (HPAIVs) of domestic poultry and wild birds has spread to more than 60 countries in Eurasia and Africa. It is concerned that HPAIVs may be perpetuated in the lakes in Siberia where migratory water birds nest in summer. To monitor whether HPAIVs ci...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-65

    authors: Yamamoto N,Sakoda Y,Motoshima M,Yoshino F,Soda K,Okamatsu M,Kida H

    更新日期:2011-02-11 00:00:00

  • Replication interference between human papillomavirus types 16 and 18 mediated by heterologous E1 helicases.

    abstract:BACKGROUND:Co-infection of multiple genotypes of human papillomavirus (HPV) is commonly observed among women with abnormal cervical cytology, but how different HPVs interact with each other in the same cell is not clearly understood. A previous study using cultured keratinocytes revealed that genome replication of one ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-11-11

    authors: Mori S,Kusumoto-Matsuo R,Ishii Y,Takeuchi T,Kukimoto I

    更新日期:2014-01-24 00:00:00

  • Antenatal HIV-1 RNA load and timing of mother to child transmission; a nested case-control study in a resource poor setting.

    abstract:OBJECTIVE:To determine HIV-1 RNA load during the third trimester of pregnancy and evaluate its effect on in utero and intra-partum/postpartum transmissions in a breastfeeding population. DESIGN:A nested case-control study within a PMTCT cohort of antiretroviral therapy naive pregnant women and their infants. METHODS:...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-176

    authors: Duri K,Gumbo FZ,Kristiansen KI,Kurewa NE,Mapingure MP,Rusakaniko S,Chirenje MZ,Muller F,Stray-Pedersen B

    更新日期:2010-08-02 00:00:00

  • HuR binding to AU-rich elements present in the 3' untranslated region of Classical swine fever virus.

    abstract:BACKGROUND:Classical swine fever virus (CSFV) is the member of the genus Pestivirus under the family Flaviviridae. The 5' untranslated region (UTR) of CSFV contains the IRES, which is a highly structured element that recruits the translation machinery. The 3' UTR is usually the recognition site of the viral replicase t...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-340

    authors: Nadar M,Chan MY,Huang SW,Huang CC,Tseng JT,Tsai CH

    更新日期:2011-07-06 00:00:00

  • Highly quantitative serological detection of anti-cytomegalovirus (CMV) antibodies.

    abstract:BACKGROUND:Human cytomegalovirus infection is associated with a variety of pathological conditions including retinitis, pneumonia, hepatitis and encephalitis that may be transmitted congenitally, horizontally and parenterally and occurs both as a primary infection and as reactivation in immunocompromised individuals. C...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-6-45

    authors: Burbelo PD,Issa AT,Ching KH,Exner M,Drew WL,Alter HJ,Iadarola MJ

    更新日期:2009-05-01 00:00:00

  • Expression of Ebolavirus glycoprotein on the target cells enhances viral entry.

    abstract:BACKGROUND:Entry of Ebolavirus to the target cells is mediated by the viral glycoprotein GP. The native GP exists as a homotrimer on the virions and contains two subunits, a surface subunit (GP1) that is involved in receptor binding and a transmembrane subunit (GP2) that mediates the virus-host membrane fusion. Previou...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-6-75

    authors: Manicassamy B,Rong L

    更新日期:2009-06-08 00:00:00

  • Identification of novel conserved functional motifs across most Influenza A viral strains.

    abstract:BACKGROUND:Influenza A virus poses a continuous threat to global public health. Design of novel universal drugs and vaccine requires a careful analysis of different strains of Influenza A viral genome from diverse hosts and subtypes. We performed a systematic in silico analysis of Influenza A viral segments of all avai...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-44

    authors: ElHefnawi M,Alaidi O,Mohamed N,Kamar M,El-Azab I,Zada S,Siam R

    更新日期:2011-01-27 00:00:00

  • Epidemics of viral meningitis caused by echovirus 6 and 30 in Korea in 2008.

    abstract:BACKGROUND:Enteroviruses (EVs) are the leading cause of aseptic meningitis, which is the most frequent central nervous system infection worldwide. We aimed to characterize the EVs involved in an aseptic meningitis outbreak in Korea in 2008. In Korea, Echovirus type 30 (E30) and E6 have been associated with outbreaks an...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-38

    authors: Kim HJ,Kang B,Hwang S,Hong J,Kim K,Cheon DS

    更新日期:2012-02-15 00:00:00

  • Epidemics of enterovirus infection in Chungnam Korea, 2008 and 2009.

    abstract:: Previously, we explored the epidemic pattern and molecular characterization of enteroviruses isolated in Chungnam, Korea from 2005 to 2006. The present study extended these observations to 2008 and 2009. In this study, enteroviruses showed similar seasonal prevalent pattern from summer to fall and age distribution to...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-297

    authors: Baek K,Yeo S,Lee B,Park K,Song J,Yu J,Rheem I,Kim J,Hwang S,Choi Y,Cheon D,Park J

    更新日期:2011-06-13 00:00:00

  • Chronic hepatitis c genotype-4 infection: role of insulin resistance in hepatocellular carcinoma.

    abstract:BACKGROUND:Hepatitis C virus (HCV) is a major cause of chronic hepatitis and hepatocellular carcinoma (HCC) and different HCV genotypes show characteristic variations in their pathological properties. Insulin resistance (IR) occurs early in HCV infection and may synergize with viral hepatitis in HCC development. Egypt ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-496

    authors: Mohamed AA,Loutfy SA,Craik JD,Hashem AG,Siam I

    更新日期:2011-11-01 00:00:00

  • Bioinformatic analysis suggests that the Orbivirus VP6 cistron encodes an overlapping gene.

    abstract:BACKGROUND:The genus Orbivirus includes several species that infect livestock - including Bluetongue virus (BTV) and African horse sickness virus (AHSV). These viruses have linear dsRNA genomes divided into ten segments, all of which have previously been assumed to be monocistronic. RESULTS:Bioinformatic evidence is p...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-5-48

    authors: Firth AE

    更新日期:2008-04-14 00:00:00

  • Molecular epidemiology of Hepatitis C virus genotypes in Khyber Pakhtoonkhaw of Pakistan.

    abstract::Six major Hepatitis C virus (HCV) genotypes and hundreds of subtypes have been identified globally. All these genotypes are generally studied for epidemiology, their vaccine development and clinical management. This article comments the frequency distribution of various HCV genotypes circulate in different areas/distr...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-203

    authors: Ali A,Ahmed H,Idrees M

    更新日期:2010-08-26 00:00:00

  • Posttranslational N-glycosylation of the hepatitis B virus large envelope protein.

    abstract:BACKGROUND:The addition of N-linked glycans to proteins is normally a cotranslational process that occurs during translocation of the nascent protein to the endoplasmic reticulum. Here, we report on an exception to this rule occurring on the hepatitis B virus (HBV) large L envelope protein that is a subject to co-plus ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-4-45

    authors: Lambert C,Prange R

    更新日期:2007-05-30 00:00:00

  • Viral dominance of reassortants between canine influenza H3N2 and pandemic (2009) H1N1 viruses from a naturally co-infected dog.

    abstract:BACKGROUND:Since avian-origin H3N2 canine influenza virus (CIV) was first identified in South Korea in 2008, the novel influenza virus has been reported in several countries in Asia. Reverse zoonotic transmission of pandemic H1N1 (2009) influenza virus (pH1N1) has been observed in a broad range of animal species. Viral...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-015-0343-z

    authors: Na W,Lyoo KS,Song EJ,Hong M,Yeom M,Moon H,Kang BK,Kim DJ,Kim JK,Song D

    更新日期:2015-09-04 00:00:00

  • Expression of chicken interleukin-2 by a highly virulent strain of Newcastle disease virus leads to decreased systemic viral load but does not significantly affect mortality in chickens.

    abstract:BACKGROUND:In mammals, interleukin 2 (IL-2) has been shown to decrease replication or attenuate pathogenicity of numerous viral pathogens (herpes simplex virus, vaccinia virus, human respiratory syncytial virus, human immunodeficiency virus) by activating natural killer cells (NK), cytotoxic T lymphocytes and expanding...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-015-0353-x

    authors: Susta L,Diel DG,Courtney S,Cardenas-Garcia S,Sundick RS,Miller PJ,Brown CC,Afonso CL

    更新日期:2015-08-08 00:00:00

  • Genomic and phylogenetic characterization of ChPV2, a novel goat PV closely related to the Xi-PV1 species infecting bovines.

    abstract:BACKGROUND:Papillomaviruses (PVs) infecting artiodactyls are very diverse, and only second in number to PVs infecting primates. PVs associated to lesions in economically important ruminant species have been isolated from cattle and sheep. METHODS:Potential PV DNA from teat lesions of a Damascus goat was isolated, clon...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-020-01440-9

    authors: Willemsen A,van den Boom A,Dietz J,Bilge Dagalp S,Dogan F,Bravo IG,Ehrhardt A,Ehrke-Schulz E

    更新日期:2020-10-30 00:00:00

  • Molecular characterization of partial fusion gene and C-terminus extension length of haemagglutinin-neuraminidase gene of recently isolated Newcastle disease virus isolates in Malaysia.

    abstract:BACKGROUND:Newcastle disease (ND), caused by Newcastle disease virus (NDV), is a highly contagious disease of birds and has been one of the major causes of economic losses in the poultry industry. Despite routine vaccination programs, sporadic cases have occasionally occurred in the country and remain a constant threat...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-183

    authors: Berhanu A,Ideris A,Omar AR,Bejo MH

    更新日期:2010-08-08 00:00:00

  • No observed effect of homologous recombination on influenza C virus evolution.

    abstract::The occurrence of homologous recombination in influenza viruses has been under some debate recently. To determine the extent of homologous recombination in influenza C virus, recombination analyses of all available gene sequences of influenza C virus were carried out. No recombination signal was found. With the previo...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-227

    authors: Han GZ,Boni MF,Li SS

    更新日期:2010-09-14 00:00:00

  • Characterization of hepatitis C Virus genotype 3a hypervariable region 1 in patients achieved rapid virological response to alpha interferon and ribavirin combination therapy.

    abstract:BACKGROUND:Hepatitis C virus roots a chronic liver disease. Currently approved treatment strategy includes administration of alpha interferon and ribavirin combined therapy for 24-48 weeks. One of the predictor of sustained virological response is an early virological response to treatment characterized as rapid respon...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-253

    authors: Akram M,Idrees M,Hussain A,Afzal S,Ilyas M,Zafar S,Aftab M,Badar S,Khubaib B

    更新日期:2011-05-23 00:00:00

  • Properties of virion transactivator proteins encoded by primate cytomegaloviruses.

    abstract:BACKGROUND:Human cytomegalovirus (HCMV) is a betaherpesvirus that causes severe disease in situations where the immune system is immature or compromised. HCMV immediate early (IE) gene expression is stimulated by the virion phosphoprotein pp71, encoded by open reading frame (ORF) UL82, and this transactivation activity...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-6-65

    authors: Nicholson IP,Sutherland JS,Chaudry TN,Blewett EL,Barry PA,Nicholl MJ,Preston CM

    更新日期:2009-05-27 00:00:00

  • Recombination analysis of Soybean mosaic virus sequences reveals evidence of RNA recombination between distinct pathotypes.

    abstract::RNA recombination is one of the two major factors that create RNA genome variability. Assessing its incidence in plant RNA viruses helps understand the formation of new isolates and evaluate the effectiveness of crop protection strategies. To search for recombination in Soybean mosaic virus (SMV), the causal agent of ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-5-143

    authors: Gagarinova AG,Babu M,Strömvik MV,Wang A

    更新日期:2008-11-26 00:00:00