Abstract:
:Filamentous bacteriophages have been used in numerous applications for the display of antibodies and random peptide libraries. Here we describe the introduction of a 13 amino acid sequence LASIFEAQKIEWR (designated BT, which is biotinylated in vivo by E. coli) into the N termini of four of the five structural proteins of the filamentous bacteriophage fd (Proteins 3, 7, 8 and 9). The in vivo and in vitro biotinylation of the various phages were compared. The production of multifunctional phages and their application as affinity reagents are demonstrated.
journal_name
Virol Jjournal_title
Virology journalauthors
Smelyanski L,Gershoni JMdoi
10.1186/1743-422X-8-495subject
Has Abstractpub_date
2011-11-01 00:00:00pages
495issn
1743-422Xpii
1743-422X-8-495journal_volume
8pub_type
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