Abstract:
BACKGROUND:Human cytomegalovirus (HCMV) is a betaherpesvirus that causes severe disease in situations where the immune system is immature or compromised. HCMV immediate early (IE) gene expression is stimulated by the virion phosphoprotein pp71, encoded by open reading frame (ORF) UL82, and this transactivation activity is important for the efficient initiation of viral replication. It is currently recognized that pp71 acts to overcome cellular intrinsic defences that otherwise block viral IE gene expression, and that interactions of pp71 with the cell proteins Daxx and ATRX are important for this function. A further property of pp71 is the ability to enable prolonged gene expression from quiescent herpes simplex virus type 1 (HSV-1) genomes. Non-human primate cytomegaloviruses encode homologs of pp71, but there is currently no published information that addresses their effects on gene expression and modes of action. RESULTS:The UL82 homolog encoded by simian cytomegalovirus (SCMV), strain Colburn, was identified and cloned. This ORF, named S82, was cloned into an HSV-1 vector, as were those from baboon, rhesus monkey and chimpanzee cytomegaloviruses. The use of an HSV-1 vector enabled expression of the UL82 homologs in a range of cell types, and permitted investigation of their abilities to direct prolonged gene expression from quiescent genomes. The results show that all UL82 homologs activate gene expression, and that neither host cell type nor promoter target sequence has major effects on these activities. Surprisingly, the UL82 proteins specified by non-human primate cytomegaloviruses, unlike pp71, did not direct long term expression from quiescent HSV-1 genomes. In addition, significant differences were observed in the intranuclear localization of the UL82 homologs, and in their effects on Daxx. Strikingly, S82 mediated the release of Daxx from nuclear domain 10 substructures much more rapidly than pp71 or the other proteins tested. All UL82 homologs stimulated the early release of ATRX from nuclear domain 10. CONCLUSION:All of the UL82 homolog proteins analysed activated gene expression, but surprising differences in other aspects of their properties were revealed. The results provide new information on early events in infection with cytomegaloviruses.
journal_name
Virol Jjournal_title
Virology journalauthors
Nicholson IP,Sutherland JS,Chaudry TN,Blewett EL,Barry PA,Nicholl MJ,Preston CMdoi
10.1186/1743-422X-6-65subject
Has Abstractpub_date
2009-05-27 00:00:00pages
65issn
1743-422Xpii
1743-422X-6-65journal_volume
6pub_type
杂志文章相关文献
Virology Journal文献大全abstract:BACKGROUND:The viral genome of hepatitis C virus constitutes a 9.6-kb single-stranded positive-sense RNA which encodes altogether 11 viral proteins. In order to study the humoral immune responses against different HCV proteins in patients suffering from chronic HCV infection, we produced three structural (core, E1 and ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-84
更新日期:2009-06-23 00:00:00
abstract::Hepatitis C virus (HCV) is endemic worldwide, and it causes cirrhosis and other complications that often lead to death; nevertheless, our knowledge of the disease and its mechanisms is limited. HCV is most common in underdeveloped nations, including many in Africa and Asia. The virus is usually transmitted by parenter...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/1743-422X-9-57
更新日期:2012-03-02 00:00:00
abstract:BACKGROUND:The epidemic pattern of respiratory syncytial virus (RSV) is quite different in regions of Europe (biennial epidemics in alternating cycles of approximately 9 and 15 months) than in the Western Hemisphere (annual epidemics). In order to determine if these differences are accounted for by the circulation of d...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-133
更新日期:2009-09-07 00:00:00
abstract:BACKGROUND:Little is known at the molecular level concerning the differences and/or similarities between alcohol and hepatitis C virus induced liver disease. Global transcriptional profiling using oligonucleotide microarrays was therefore performed on liver biopsies from patients with cirrhosis caused by either chronic...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-3-98
更新日期:2006-11-22 00:00:00
abstract:BACKGROUND:Torque teno virus is a small chronically persisting circular negative ssDNA virus reaching near 100% prevalence. It is reported to be a marker for immune function in immunocompromised patients. The possibility of vertical maternal-fetal transmission remains controversial but incidence rate of TTV DNA in chil...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-018-1007-6
更新日期:2018-05-30 00:00:00
abstract:BACKGROUND:Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes acute viral encephalitis in humans. Pigs are important amplifier hosts of JEV. Emerging evidence indicates that host microRNAs (miRNAs) play key roles in modulating viral infection and pathogenesis. However, mechanistic studies deli...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0562-y
更新日期:2016-06-21 00:00:00
abstract::Cyclooxygenases (COXs) play a significant role in many different viral infections with respect to replication and pathogenesis. Here we investigated the role of COXs in the mouse hepatitis coronavirus (MHV) infection cycle. Blocking COX activity by different inhibitors or by RNA interference affected MHV infection in ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-4-55
更新日期:2007-06-07 00:00:00
abstract:BACKGROUND:Co-circulation of multiple dengue virus serotypes has been reported from many parts of the world including India, however concurrent infection with more than one serotype of dengue viruses in the same individual is rarely documented. An outbreak of dengue hemorrhagic fever/dengue shock syndrome (DHF/DSS) occ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-1
更新日期:2008-01-09 00:00:00
abstract:BACKGROUND:The recently sequenced 218 kb genome of morphologically atypical Bacillus thuringiensis phage 0305phi8-36 exhibited only limited detectable homology to known bacteriophages. The only known relative of this phage is a string of phage-like genes called BtI1 in the chromosome of B. thuringiensis israelensis. Th...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-4-97
更新日期:2007-10-05 00:00:00
abstract:BACKGROUND:Chikungunya virus (CHIKV) is a mosquito-borne, arthrogenic Alphavirus that causes large epidemics in Africa, South-East Asia and India. Recently, CHIKV has been transmitted to humans in Southern Europe by invading and now established Asian tiger mosquitoes. To study the processing of envelope proteins E1 and...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-353
更新日期:2011-07-15 00:00:00
abstract::Marek's disease is a highly contagious disease of poultry characterized by rapid-on set of T-cell lymphomas, which is caused by Marek's disease virus (MDV), but its pathogenic mechanism is still not very clear. Recently, some new progress were achieved in molecular character of MDV. Along with the genomic sequencing o...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-371
更新日期:2010-12-23 00:00:00
abstract::Virology Journal is an exclusively on-line, Open Access journal devoted to the presentation of high-quality original research concerning human, animal, plant, insect bacterial, and fungal viruses. Virology Journal will establish a strategic alternative to the traditional virology communication process. ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-1-1
更新日期:2004-08-26 00:00:00
abstract:BACKGROUND:The Capripoxvirus, Lumpy skin disease virus (LSDV) has a restricted host-range and is being investigated as a novel HIV-1 vaccine vector. LSDV does not complete its replication cycle in non-ruminant hosts. METHODS:The safety of LSDV was tested at doses of 104 and 106 plaque forming units in two strains of i...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-265
更新日期:2011-05-30 00:00:00
abstract:BACKGROUND/AIM:Hepatitis C virus (HCV) has been the subject of intense research and clinical investigation as its major role in human disease has emerged. HCV circulates in vivo as a complex population of different but closely related viral variants, commonly referred to as a quasispecies. The extent to which recombina...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-3-87
更新日期:2006-10-24 00:00:00
abstract:BACKGROUND:HIV-1 is highly variable genetically and at protein level, a property it uses to subvert antiviral immunity and treatment. The aim of this study was to assess if HIV subtype differences were associated with variations in glycosylation patterns and co-receptor tropism among HAART patients experiencing differe...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-017-0703-y
更新日期:2017-02-14 00:00:00
abstract:BACKGROUND:Inteins are "protein introns" that remove themselves from their host proteins through an autocatalytic protein-splicing. After their discovery, inteins have been quickly identified in all domains of life, but only once to date in the genome of a eukaryote-infecting virus. RESULTS:Here we report the identifi...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-2-8
更新日期:2005-02-11 00:00:00
abstract:BACKGROUND:Recombinant Salmonella vaccine vectors may potentially be used to induce specific CD4+ T cell responses against foreign viral antigens. Such immune responses are required features of vaccines against pathogens such as human immunodeficiency virus type 1 (HIV-1). The aim of this study was to investigate the i...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-87
更新日期:2009-06-25 00:00:00
abstract::From April 2007 to March 2008, cloacal swabs were obtained from 246 casualty raptors recovered by various wildlife rehabilitation centers in the United States. The swabs were placed in a virus transport medium and transported to the laboratory on ice packs. At the laboratory, the samples were pooled with each pool con...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-174
更新日期:2010-07-28 00:00:00
abstract:BACKGROUND:Bovine leukemia virus (BLV), which is closely related to human T-cell leukemia virus, is the etiological agent of enzootic bovine leukosis, a disease characterized by a highly prolonged course involving persistent lymphocytosis and B-cell lymphoma. The bovine major histocompatibility complex class II region ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-019-1259-9
更新日期:2019-12-16 00:00:00
abstract:BACKGROUND:The Epstein-Barr virus (EBV) is associated with lymphoid malignancies, including Burkitt's lymphoma (BL), and can transform human B cells in vitro. EBV-harboring cell lines are widely used to investigate lymphocyte transformation and oncogenesis. Qualitative EBV gene expression has been extensively described...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-3-43
更新日期:2006-06-06 00:00:00
abstract:BACKGROUND:Aleutian mink disease virus (AMDV) is the cause of a chronic immune complex disease, Aleutian disease (AD), which is common in mink-producing countries. In 2005, implementation of an AMDV eradication programme in Finland created a need for an automated high-throughput assay. The aim of this study was to vali...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-141
更新日期:2014-08-08 00:00:00
abstract:BACKGROUND:The aquatic birnavirus infectious pancreatic necrosis virus (IPNV) causes infectious pancreatic necrosis (IPN), a severe disease in farmed salmonid fish. IPNV has a very broad host range and infects many different species of fish as well as molluscs and crustaceans. Investigation of the host reservoir of a v...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-228
更新日期:2012-10-05 00:00:00
abstract:BACKGROUND:Human caliciviruses (HuCV) are emerging enteric pathogens that are a common cause of diarrhea in humans worldwide. Due to the paucity of information on the molecular characterization of HuCV circulating in Mexico, the aim of this work was to investigate the diversity and molecular epidemiology of the HuCV in...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-54
更新日期:2012-02-23 00:00:00
abstract:BACKGROUND:At the moment, there are a number of publications describing gene expression profiling in virus-infected plants. Most of the data are limited to specific host-pathogen interactions involving a given virus and a model host plant - usually Arabidopsis thaliana. Even though several summarizing attempts have bee...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-101
更新日期:2012-05-29 00:00:00
abstract::The occurrence of homologous recombination in influenza viruses has been under some debate recently. To determine the extent of homologous recombination in influenza C virus, recombination analyses of all available gene sequences of influenza C virus were carried out. No recombination signal was found. With the previo...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-7-227
更新日期:2010-09-14 00:00:00
abstract:BACKGROUND:Human papillomavirus (HPV) has a causal role in cervical cancer with almost half a million new cases occurring each year. Presence of the carcinogenic HPV is necessary for the development of the invasive carcinoma of the genital tract. Therefore, persistent infection with carcinogenic HPV causes virtually al...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-4-33
更新日期:2007-03-28 00:00:00
abstract:BACKGROUND:The deciphering of cellular networks to determine susceptibility to infection by HIV or the related simian immunodeficiency virus (SIV) is a major challenge in infection biology. RESULTS:Here, we have compared gene expression profiles of a human CD4+ T cell line at 24 h after infection with a cell line of t...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-11-152
更新日期:2014-08-27 00:00:00
abstract:BACKGROUND:Both regulatory T cells (Tregs) and PD-1/PD-L1 pathway were critically involved in HCV viral persistence. However, the association between them was not well investigated. Herein, we aimed to investigate the distributional profiles of Tregs subsets and association between PD-1 expression on these subsets and ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-279
更新日期:2011-06-07 00:00:00
abstract:BACKGROUND:West Nile virus (WNV) is an emerging zoonotic pathogen which is harmful to human and animal health. Effective vaccination in susceptible hosts should protect against WNV infection and significantly reduce viral transmission between animals and from animals to humans. A versatile vaccine suitable for differen...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0568-5
更新日期:2016-06-24 00:00:00
abstract:BACKGROUND:The context and purpose of the study included 1) bacterial expression of viral protein 6 (VP6) of porcine rotavirus (PRV) and generation of rabbit polyclonal antiserum to the VP6 protein; 3) establishment of a discrimination ELISA to distinguish PRV from a panel of other porcine viruses. RESULTS:The VP6 gen...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-91
更新日期:2013-03-22 00:00:00