Isolation of mixed subtypes of influenza A virus from a bald eagle (Haliaeetus leucocephalus).

Abstract:

:From April 2007 to March 2008, cloacal swabs were obtained from 246 casualty raptors recovered by various wildlife rehabilitation centers in the United States. The swabs were placed in a virus transport medium and transported to the laboratory on ice packs. At the laboratory, the samples were pooled with each pool consisting of five samples. All pools (n = 50) were screened for the presence of avian influenza virus (AIV) using a real time reverse transcription-polymerase chain reaction (rRT-PCR); one of the pools was found positive. All five samples in this pool were tested individually by rRT-PCR; one sample from a bald eagle was found positive. This sample was inoculated in embryonated chicken eggs for virus isolation and a hemagglutinating virus was isolated. Complete genome sequencing of the isolate revealed a mixed infection with H1N1 and H2N1 subtypes. Further analysis revealed that the PB1-F2 gene sequence of H1N1 virus had the N66S virulence-associated substitution. Further studies on ecology and epidemiology of AIV in raptors are needed to help understand their role in the maintenance and evolution of AIV.

journal_name

Virol J

journal_title

Virology journal

authors

Goyal SM,Jindal N,Chander Y,Ramakrishnan MA,Redig PT,Sreevatsan S

doi

10.1186/1743-422X-7-174

subject

Has Abstract

pub_date

2010-07-28 00:00:00

pages

174

issn

1743-422X

pii

1743-422X-7-174

journal_volume

7

pub_type

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