Hepatitis B virus infection and replication in human bone marrow mesenchymal stem cells.

Abstract:

BACKGROUND:Hepatitis B virus (HBV) infection is a blood borne infectious disease that affects the liver. Human bone marrow mesenchymal stem cells (BMSCs) may serve as a cell source for adult stem cell transplantation in liver repair. However, the susceptibility of human BMSCs to HBV infection is poorly understood. The aim of this study was to investigate the infection and replication of HBV in cultures of human BMSCs. RESULTS:Human BMSCs were confirmed using flow cytometry. Intracellular HBV DNA was detected at d 2 after infection and maintained at relatively high levels from d 6 to d 12. The maximal level of intracellular HBV DNA was 9.37 × 105 copies/mL. The extracellular HBV DNA was observed from d 3 to d 15, and the levels ranged from 3.792 × 102 copies/mL to 4.067 × 105 copies/mL. HBsAg in the culture medium was detected from d 2 to d 16. HBeAg secretion was positive from d 5 to d 13. HBcAg constantly showed positive signals in approximately 7%-20% of BMSCs from 2 days after exposure. Intracellular HBV covalently closed circular DNA (cccDNA) could be detected as early as 2 days postinfection, and strong signals were obtained with increasing time. CONCLUSION:HBV can infect and replicate in human BMSCs. Human BMSCs may be a useful tool for investigating HBV life-cycle and the mechanism of initial virus-cell interactions.

journal_name

Virol J

journal_title

Virology journal

authors

Ma R,Xing Q,Shao L,Wang D,Hao Q,Li X,Sai L,Ma L

doi

10.1186/1743-422X-8-486

subject

Has Abstract

pub_date

2011-10-31 00:00:00

pages

486

issn

1743-422X

pii

1743-422X-8-486

journal_volume

8

pub_type

杂志文章
  • Potential effects of telbivudine and entecavir on renal function: a systematic review and meta-analysis.

    abstract:BACKGROUND:To assess the potential effects of telbivudine (LdT) and entecavir (ETV) on renal function in patients with chronic hepatitis B (CHB), we performed a meta-analysis of the relevant data available on these agents to evaluate their effects on the estimated glomerular filtration rate (eGFR) during treatment. ME...

    journal_title:Virology journal

    pub_type: 杂志文章,meta分析,评审

    doi:10.1186/s12985-016-0522-6

    authors: Wu X,Cai S,Li Z,Zheng C,Xue X,Zeng J,Peng J

    更新日期:2016-04-09 00:00:00

  • Transmission of H7N9 influenza virus in mice by different infective routes.

    abstract:BACKGROUND:On 19 February 2013, the first patient infected with a novel influenza A H7N9 virus from an avian source showed symptoms of sickness. More than 349 laboratory-confirmed cases and 109 deaths have been reported in mainland China since then. Laboratory-confirmed, human-to-human H7N9 virus transmission has not b...

    journal_title:Virology journal

    pub_type: 历史文章,杂志文章

    doi:10.1186/1743-422X-11-185

    authors: Bao L,Xu L,Zhu H,Deng W,Chen T,Lv Q,Li F,Yuan J,Xu Y,Huang L,Li Y,Liu J,Yao Y,Yu P,Chen H,Qin C

    更新日期:2014-11-03 00:00:00

  • The dual role of tetraspanin CD63 in HIV-1 replication.

    abstract:BACKGROUND:Previously, we showed that the tetraspanin membrane protein CD63 mediates both early and post-integration stages of the HIV-1 replication cycle. The temporal roles of CD63 were discerned using monoclonal antibodies and small interfering RNAs (siRNAs) to block CD63 function, and determining which of the seque...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-11-23

    authors: Li G,Endsley MA,Somasunderam A,Gbota SL,Mbaka MI,Murray JL,Ferguson MR

    更新日期:2014-02-08 00:00:00

  • Extended phylogenetic analysis of a new Israeli isolate of Brevicoryne brassicae virus (BrBV-IL) suggests taxonomic revision of the genus Iflavirus.

    abstract:BACKGROUND:Brevicoryne brassicae virus (BrBV) is a positive-strand genomic RNA virus which is unassigned tentative member of the genus Iflavirus. BrBv was first identified and characterized in the late 90's in the cabbage aphid in the United Kingdom (UK) (J Gen Virol 88:2590-2595, 2007) and was fully sequenced, using r...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-016-0500-z

    authors: Luria N,Reingold V,Lachman O,Sela N,Dombrovsky A

    更新日期:2016-03-22 00:00:00

  • Kinetics of pulmonary immune cells, antibody responses and their correlations with the viral clearance of influenza A fatal infection in mice.

    abstract::Fatal influenza A virus infection is a major threat to public health throughout the world. Lung macrophages and neutrophils have critical roles for both the pathogenesis and viral clearance of fatal viral infections. These are complicated by the interaction of innate immunity and adaptive immunity against viral infect...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-11-57

    authors: Lv J,Hua Y,Wang D,Liu A,An J,Li A,Wang Y,Wang X,Jia N,Jiang Q

    更新日期:2014-03-26 00:00:00

  • Comparative analysis of microarray data in Arabidopsis transcriptome during compatible interactions with plant viruses.

    abstract:BACKGROUND:At the moment, there are a number of publications describing gene expression profiling in virus-infected plants. Most of the data are limited to specific host-pathogen interactions involving a given virus and a model host plant - usually Arabidopsis thaliana. Even though several summarizing attempts have bee...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-101

    authors: Postnikova OA,Nemchinov LG

    更新日期:2012-05-29 00:00:00

  • A highly divergent South African geminivirus species illuminates the ancient evolutionary history of this family.

    abstract:BACKGROUND:We have characterised a new highly divergent geminivirus species, Eragrostis curvula streak virus (ECSV), found infecting a hardy perennial South African wild grass. ECSV represents a new genus-level geminivirus lineage, and has a mixture of features normally associated with other specific geminivirus genera...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-6-36

    authors: Varsani A,Shepherd DN,Dent K,Monjane AL,Rybicki EP,Martin DP

    更新日期:2009-03-25 00:00:00

  • Antibody-dependent infection of human macrophages by severe acute respiratory syndrome coronavirus.

    abstract:BACKGROUND:Public health risks associated to infection by human coronaviruses remain considerable and vaccination is a key option for preventing the resurgence of severe acute respiratory syndrome coronavirus (SARS-CoV). We have previously reported that antibodies elicited by a SARS-CoV vaccine candidate based on recom...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-11-82

    authors: Yip MS,Leung NH,Cheung CY,Li PH,Lee HH,Daëron M,Peiris JS,Bruzzone R,Jaume M

    更新日期:2014-05-06 00:00:00

  • Simultaneous detection of novel H7N9 and other influenza A viruses in poultry by multiplex real-time RT-PCR.

    abstract:BACKGROUND:A novel reassortant H7N9 influenza A virus has crossed the species barrier from poultry to cause human infections in China in 2013 and 2014. Rapid detection of the novel H7N9 virus is important to detect this virus in poultry and reduce the risk of an epidemic in birds or humans. FINDINGS:In this study, a m...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-015-0300-x

    authors: Xu X,Bao H,Ma Y,Sun J,Zhao Y,Wang Y,Shi J,Zeng X,Li Y,Wang X,Chen H

    更新日期:2015-04-30 00:00:00

  • Characterisation of the epidemic strain of H3N8 equine influenza virus responsible for outbreaks in South America in 2012.

    abstract:BACKGROUND:An extensive outbreak of equine influenza occurred across multiple countries in South America during 2012. The epidemic was first reported in Chile then spread to Brazil, Uruguay and Argentina, where both vaccinated and unvaccinated animals were affected. In Brazil, infections were widespread within 3months ...

    journal_title:Virology journal

    pub_type: 历史文章,杂志文章

    doi:10.1186/s12985-016-0503-9

    authors: Alves Beuttemmüller E,Woodward A,Rash A,Dos Santos Ferraz LE,Fernandes Alfieri A,Alfieri AA,Elton D

    更新日期:2016-03-19 00:00:00

  • Subcellular localization and interactions of Infectious Salmon Anemia Virus (ISAV) M1 and NEP as well as host Hsc70.

    abstract:BACKGROUND:Infectious salmon anemia virus (ISAV) is an important fish pathogen that causes high mortality in farmed Atlantic salmon. The ISAV genome consists of eight single-stranded, negative-sense RNA segments. The six largest segments contain one open reading frame (ORF) each, and encode three polymerase proteins, n...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-017-0702-z

    authors: Zhang W,Cai C,Lin L,Tao YJ,Jin M

    更新日期:2017-02-15 00:00:00

  • Association of LIN28B polymorphisms with chronic hepatitis B virus infection.

    abstract:BACKGROUND:LIN28B is involved in multiple cellular developmental processes, tissue inflammatory response and tumourigenesis. The association of LIN28B polymorphisms with hepatitis B virus (HBV) infection remains unknown. METHODS:This study investigated the association of LIN28B rs314277, rs314280, rs369065 and rs77599...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-020-01353-7

    authors: Han Q,Sang J,Fan X,Wang X,Zeng L,Zhang X,Zhang K,Li N,Lv Y,Liu Z

    更新日期:2020-06-22 00:00:00

  • Identification of mumps virus protein and lipid composition by mass spectrometry.

    abstract:BACKGROUND:Mumps virus is a negative-sense, single stranded RNA virus consisting of a ribonucleocapsid core enveloped by a lipid membrane derived from host cell, which causes mumps disease preventable by vaccination. Since virus lipid envelope and glycosylation pattern are not encoded by the virus but dependent on the ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-016-0463-0

    authors: Brgles M,Bonta M,Šantak M,Jagušić M,Forčić D,Halassy B,Allmaier G,Marchetti-Deschmann M

    更新日期:2016-01-14 00:00:00

  • Virology on the Internet: the time is right for a new journal.

    abstract::Virology Journal is an exclusively on-line, Open Access journal devoted to the presentation of high-quality original research concerning human, animal, plant, insect bacterial, and fungal viruses. Virology Journal will establish a strategic alternative to the traditional virology communication process. ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-1-1

    authors: Garry RF

    更新日期:2004-08-26 00:00:00

  • Interaction between Bluetongue virus outer capsid protein VP2 and vimentin is necessary for virus egress.

    abstract:BACKGROUND:The VP2 outer capsid protein Bluetongue Virus (BTV) is responsible for receptor binding, haemagglutination and eliciting host-specific immunity. However, the assembly of this outer capsid protein on the transcriptionally active viral core would block transcription of the virus. Thus assembly of the outer cap...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-4-7

    authors: Bhattacharya B,Noad RJ,Roy P

    更新日期:2007-01-15 00:00:00

  • Long-term nucleos(t)ide analogues therapy for adults with chronic hepatitis B reduces the risk of long-term complications: a meta-analysis.

    abstract:BACKGROUND:The effect of antiviral therapy in chronic hepatitis B (CHB) on reducing the risk of long-term complications (LTCs) remains unclear so far. To study whether long-term nucleos(t)ide analogues therapy can reduce the risk of long-term complications. METHODS:We searched MEDLINE, EMBASE, OVID, the Cochrane Centr...

    journal_title:Virology journal

    pub_type: 杂志文章,meta分析,评审

    doi:10.1186/1743-422X-8-72

    authors: Zhang QQ,An X,Liu YH,Li SY,Zhong Q,Wang J,Hu HD,Zhang DZ,Ren H,Hu P

    更新日期:2011-02-15 00:00:00

  • Kinetics of antibody-induced modulation of respiratory syncytial virus antigens in a human epithelial cell line.

    abstract:BACKGROUND:The binding of viral-specific antibodies to cell-surface antigens usually results in down modulation of the antigen through redistribution of antigens into patches that subsequently may be internalized by endocytosis or may form caps that can be expelled to the extracellular space. Here, by use of confocal-l...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-4-68

    authors: Sarmiento RE,Tirado RG,Valverde LE,Gómez-Garcia B

    更新日期:2007-07-03 00:00:00

  • Antenatal HIV-1 RNA load and timing of mother to child transmission; a nested case-control study in a resource poor setting.

    abstract:OBJECTIVE:To determine HIV-1 RNA load during the third trimester of pregnancy and evaluate its effect on in utero and intra-partum/postpartum transmissions in a breastfeeding population. DESIGN:A nested case-control study within a PMTCT cohort of antiretroviral therapy naive pregnant women and their infants. METHODS:...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-176

    authors: Duri K,Gumbo FZ,Kristiansen KI,Kurewa NE,Mapingure MP,Rusakaniko S,Chirenje MZ,Muller F,Stray-Pedersen B

    更新日期:2010-08-02 00:00:00

  • Post-transplant lymphoproliferative disorders and Epstein-Barr virus DNAemia in a cohort of lung transplant recipients.

    abstract:BACKGROUND:Post-transplant lymphoproliferative disorders (PTLD) are serious complications in lung transplant recipients. No consensus on EBV DNAemia levels predictive of PTLD has been reached. In addition, in many instances EBV DNAemia is determined in patients with suggestive symptoms only. METHODS:The characteristic...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-421

    authors: Baldanti F,Rognoni V,Cascina A,Oggionni T,Tinelli C,Meloni F

    更新日期:2011-09-05 00:00:00

  • Comparative analyses of host responses upon infection with moderately virulent classical swine fever virus in domestic pigs and wild boar.

    abstract:BACKGROUND:Classical swine fever (CSF) is one of the most important viral diseases of pigs. Clinical signs may vary from almost inapparent infection to a hemorrhagic fever like illness. Among the host factors leading to different disease courses are age, breed, and immune status. The aim of this study was to compare ho...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-11-134

    authors: Petrov A,Blohm U,Beer M,Pietschmann J,Blome S

    更新日期:2014-07-29 00:00:00

  • Capsid display of a conserved human papillomavirus L2 peptide in the adenovirus 5 hexon protein: a candidate prophylactic hpv vaccine approach.

    abstract:BACKGROUND:Infection by any one of 15 high risk human papillomavirus (hrHPV) types causes most invasive cervical cancers. Their oncogenic genome is encapsidated by L1 (major) and L2 (minor) coat proteins. Current HPV prophylactic vaccines are composed of L1 virus-like particles (VLP) that elicit type restricted immunit...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-015-0364-7

    authors: Wu WH,Alkutkar T,Karanam B,Roden RB,Ketner G,Ibeanu OA

    更新日期:2015-09-11 00:00:00

  • Identification of novel human adenovirus candidates using the coxsackievirus and adenovirus receptor for cell entry.

    abstract:BACKGROUND:There are over 100 known human adenovirus (HAdV) types, which are able to cause a broad variety of different self-limiting but also lethal diseases especially in immunocompromised patients. Only limited information about the pathogenesis and biology of the majority of these virus types is available. In the p...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-020-01318-w

    authors: Mese K,Bunz O,Schellhorn S,Volkwein W,Jung D,Gao J,Zhang W,Baiker A,Ehrhardt A

    更新日期:2020-04-09 00:00:00

  • The new temperature-sensitive mutation PA-F35S for developing recombinant avian live attenuated H5N1 influenza vaccine.

    abstract:BACKGROUND:H5N1 highly pathogenic avian influenza virus (HPAIV) is continuously circulating in many Asian countries and threatening poultry industry and human population. Vaccination is the best strategy to control H5N1 HPAIV infection in poultry and transmission to human population. The aim of this study is to identif...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-97

    authors: Zhang W,Tu J,Zhao Z,Chen H,Jin M

    更新日期:2012-05-23 00:00:00

  • Antibody dependent enhancement of frog virus 3 infection.

    abstract:BACKGROUND:Viruses included in the family Iridoviridae are large, icosahedral, dsDNA viruses that are subdivided into 5 genera. Frog virus 3 (FV3) is the type species of the genus Ranavirus and the best studied iridovirus at the molecular level. Typically, antibodies directed against a virus act to neutralize the virus...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-41

    authors: Eaton HE,Penny E,Brunetti CR

    更新日期:2010-02-18 00:00:00

  • Evidence of recombination within human alpha-papillomavirus.

    abstract:BACKGROUND:Human papillomavirus (HPV) has a causal role in cervical cancer with almost half a million new cases occurring each year. Presence of the carcinogenic HPV is necessary for the development of the invasive carcinoma of the genital tract. Therefore, persistent infection with carcinogenic HPV causes virtually al...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-4-33

    authors: Angulo M,Carvajal-Rodríguez A

    更新日期:2007-03-28 00:00:00

  • Prevalence of human pegivirus-1 and sequence variability of its E2 glycoprotein estimated from screening donors of fetal stem cell-containing material.

    abstract:BACKGROUND:Human pegivirus-1 (HPgV-1) is a member of the Flaviviridae family whose genomic organization and mode of cellular entry is similar to that of hepatitis C virus (HCV). The E2 glycoprotein of HPgV-1 is the principle mediator in the virus-cell interaction and as such harbors most of HPgV-1's antigenic determina...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-017-0837-y

    authors: Vitrenko Y,Kostenko I,Kulebyakina K,Sorochynska K

    更新日期:2017-08-31 00:00:00

  • Species and age related differences in the type and distribution of influenza virus receptors in different tissues of chickens, ducks and turkeys.

    abstract::We undertook one of the most detailed studies on the distribution of alpha2,3 sialic acid (SA)-galactose (gal) (avian type) and alpha2,6SA-gal (human type) receptors on different tissues of chickens, ducks and turkeys of varying age groups. On the tracheal epithelium, all 3 bird species expressed strong positive stain...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-5

    authors: Pillai SP,Lee CW

    更新日期:2010-01-12 00:00:00

  • Analysis of the 5'UTR of HCV genotype 3 grown in vitro in human B cells, T cells, and macrophages.

    abstract:BACKGROUND:Previously, we have reported the isolation and molecular characterization of human Hepatitis C virus genotype 1 (HCV-1) from infected patients. We are now reporting an analysis of HCV obtained from patients infected with HCV genotype 3 (HCV-3) as diagnosed by clinical laboratories. RESULTS:HCV was cultured ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-155

    authors: Revie D,Alberti MO,Prichard JG,Kelley AS,Salahuddin SZ

    更新日期:2010-07-13 00:00:00

  • The directionality of the nuclear transport of the influenza A genome is driven by selective exposure of nuclear localization sequences on nucleoprotein.

    abstract:BACKGROUND:Early in infection, the genome of the influenza A virus, consisting of eight complexes of RNA and proteins (termed viral ribonucleoproteins; vRNPs), enters the nucleus of infected cells for replication. Incoming vRNPs are imported into the nucleus of infected cells using at least two nuclear localization seq...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-6-68

    authors: Wu WW,Panté N

    更新日期:2009-06-02 00:00:00

  • Replication of porcine circoviruses.

    abstract::Porcine circoviruses are circular single-stranded DNA viruses that infect swine and wild boars. Two species of porcine circoviruses exist. Porcine circovirus type 1 is non pathogenic contrary to porcine circovirus type 2 which is associated with the disease known as Post-weaning Multisystemic Wasting Syndrome. Porcine...

    journal_title:Virology journal

    pub_type: 杂志文章,评审

    doi:10.1186/1743-422X-6-60

    authors: Faurez F,Dory D,Grasland B,Jestin A

    更新日期:2009-05-18 00:00:00