Immunological and molecular epidemiological characteristics of acute and fulminant viral hepatitis A.

Abstract:

BACKGROUND:Hepatitis A virus is an infection of liver; it is hyperendemic in vast areas of the world including India. In most cases it causes an acute self limited illness but rarely fulminant. There is growing concern about change in pattern from asymptomatic childhood infection to an increased incidence of symptomatic disease in the adult population. OBJECTIVE:In-depth analysis of immunological, viral quantification and genotype of acute and fulminant hepatitis A virus. METHODS:Serum samples obtained from 1009 cases of suspected acute viral hepatitis was employed for different biochemical and serological examination. RNA was extracted from blood serum, reverse transcribed into cDNA and amplified using nested PCR for viral quantification, sequencing and genotyping. Immunological cell count from freshly collected whole blood was carried out by fluorescence activated cell sorter. RESULTS:Fulminant hepatitis A was mostly detected with other hepatic viruses. CD8+ T cells count increases in fulminant hepatitis to a significantly high level (P = 0.005) compared to normal healthy control. The immunological helper/suppressor (CD4+/CD8+) ratio of fulminant hepatitis was significantly lower compared to acute cases. The serologically positive patients were confirmed by RT-PCR and total of 72 (69.2%) were quantified and sequenced. The average quantitative viral load of fulminant cases was significantly higher (P < 0.05). There was similar genotypic distribution in both acute and fulminant category, with predominance of genotype IIIA (70%) compared to IA (30%). CONCLUSIONS:Immunological factors in combination with viral load defines the severity of the fulminant hepatitis A. Phylogenetic analysis of acute and fulminant hepatitis A confirmed genotypes IIIA as predominant against IA with no preference of disease severity.

journal_name

Virol J

journal_title

Virology journal

authors

Hussain Z,Husain SA,Almajhdi FN,Kar P

doi

10.1186/1743-422X-8-254

subject

Has Abstract

pub_date

2011-05-23 00:00:00

pages

254

issn

1743-422X

pii

1743-422X-8-254

journal_volume

8

pub_type

杂志文章
  • Differential control of CXCR4 and CD4 downregulation by HIV-1 Gag.

    abstract:BACKGROUND:The ESCRT (endosomal sorting complex required for transport) machinery functions to sort cellular receptors into the lumen of the multivesicular body (MVB) prior to lysosomal degradation. ESCRT components can also be recruited by enveloped viruses to sites of viral assembly where they have been proposed to m...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-5-23

    authors: Valiathan RR,Resh MD

    更新日期:2008-02-11 00:00:00

  • Site directed biotinylation of filamentous phage structural proteins.

    abstract::Filamentous bacteriophages have been used in numerous applications for the display of antibodies and random peptide libraries. Here we describe the introduction of a 13 amino acid sequence LASIFEAQKIEWR (designated BT, which is biotinylated in vivo by E. coli) into the N termini of four of the five structural proteins...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-495

    authors: Smelyanski L,Gershoni JM

    更新日期:2011-11-01 00:00:00

  • Genotyping of 30 kinds of cutaneous human papillomaviruses by a multiplex microfluidic loop-mediated isothermal amplification and visual detection method.

    abstract:BACKGROUND:Human papillomaviruses (HPVs), a group of non-enveloped small viruses with double-stranded circular DNA which lead to multiple skin diseases such as benign warts, are commonly seen in clinics. The current HPV detection systems aim mainly at mucosal HPVs, however, an efficient clinical approach for cutaneous ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-020-01373-3

    authors: Wang Y,Ge G,Mao R,Wang Z,Sun YZ,Du YG,Gao XH,Qi RQ,Chen HD

    更新日期:2020-07-09 00:00:00

  • No observed effect of homologous recombination on influenza C virus evolution.

    abstract::The occurrence of homologous recombination in influenza viruses has been under some debate recently. To determine the extent of homologous recombination in influenza C virus, recombination analyses of all available gene sequences of influenza C virus were carried out. No recombination signal was found. With the previo...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-227

    authors: Han GZ,Boni MF,Li SS

    更新日期:2010-09-14 00:00:00

  • Recombination in West Nile Virus: minimal contribution to genomic diversity.

    abstract::Recombination is known to play a role in the ability of various viruses to acquire sequence diversity. We consequently examined all available West Nile virus (WNV) whole genome sequences both phylogenetically and with a variety of computational recombination detection algorithms. We found that the number of distinct l...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-6-165

    authors: Pickett BE,Lefkowitz EJ

    更新日期:2009-10-12 00:00:00

  • A small molecule inhibitor of dengue virus type 2 protease inhibits the replication of all four dengue virus serotypes in cell culture.

    abstract:BACKGROUND:Dengue has emerged as the most significant of arboviral diseases in the 21st century. It is endemic to >100 tropical and sub-tropical countries around the world placing an estimated 3.6 billion people at risk. It is caused by four genetically similar but antigenically distinct, serotypes of dengue viruses. T...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-015-0248-x

    authors: Raut R,Beesetti H,Tyagi P,Khanna I,Jain SK,Jeankumar VU,Yogeeswari P,Sriram D,Swaminathan S

    更新日期:2015-02-08 00:00:00

  • New Vibrio cholerae O1 biotype ElTor bacteriophages.

    abstract::We report the presence of three new O1 ElTor vibriophages named AS1, AS2 and AS3, isolated from the sewage and pond waters of the outskirts of Kolkata. A few phages, named AS4, with hexagonal heads and abnormally long tails with typical curly projections were also found in the water samples. ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-2-28

    authors: Sen A,Ghosh AN

    更新日期:2005-04-11 00:00:00

  • Immunohistochemical insights into Saffold virus infection of the brain of juvenile AG129 mice.

    abstract:BACKGROUND:Saffold Virus (SAFV) is a human cardiovirus that is suspected of causing infection of the central nervous system (CNS) in children. While recent animal studies have started to elucidate the pathogenesis of SAFV, very little is known about the mechanisms behind it. METHOD:In this study, we attempted to eluci...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-016-0654-8

    authors: Tan SZ,Prabakaran M

    更新日期:2016-11-25 00:00:00

  • Vaccination of ferrets with a recombinant G glycoprotein subunit vaccine provides protection against Nipah virus disease for over 12 months.

    abstract:BACKGROUND:Nipah virus (NiV) is a zoonotic virus belonging to the henipavirus genus in the family Paramyxoviridae. Since NiV was first identified in 1999, outbreaks have continued to occur in humans in Bangladesh and India on an almost annual basis with case fatality rates reported between 40% and 100%. METHODS:Ferret...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-10-237

    authors: Pallister JA,Klein R,Arkinstall R,Haining J,Long F,White JR,Payne J,Feng YR,Wang LF,Broder CC,Middleton D

    更新日期:2013-07-16 00:00:00

  • A simplified in vitro ligation approach to clone an E1B55k-deleted double-targeted conditionally-replicative adenovirus.

    abstract:BACKGROUND:Construction of conditionally-replicative Adenovirus (CRAd) is complex and time-consuming. While homologous recombination (HR) using a two-plasmid system in bacteria is commonly used to generate CRAds, alternative methods may be required when HR fails. Previously, in vitro ligation has been suggested to faci...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-6-18

    authors: Haviv YS

    更新日期:2009-02-07 00:00:00

  • Donor variability in HIV binding to peripheral blood mononuclear cells.

    abstract:BACKGROUND:HIV infection of cells varies greatly between individuals, with multiple steps in the replication cycle potentially contributing to the variability. Although entry and post-entry variability of HIV infection levels in cells has been demonstrated, variability in HIV binding has not been examined. In this stud...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-5-95

    authors: Anzinger JJ,Olinger GG,Spear GT

    更新日期:2008-08-15 00:00:00

  • Identification and characterization of unrecognized viruses in stool samples of non-polio acute flaccid paralysis children by simplified VIDISCA.

    abstract:BACKGROUND:The use of sequence independent methods combined with next generation sequencing for identification purposes in clinical samples appears promising and exciting results have been achieved to understand unexplained infections. One sequence independent method, Virus Discovery based on cDNA Amplified Fragment Le...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-11-146

    authors: Shaukat S,Angez M,Alam MM,Jebbink MF,Deijs M,Canuti M,Sharif S,de Vries M,Khurshid A,Mahmood T,van der Hoek L,Zaidi SS

    更新日期:2014-08-12 00:00:00

  • Morphological evidence for phages in Xylella fastidiosa.

    abstract::Presumptive phage particles associated with Xylella fastidiosa strain Temecula-1 grown in PW broth were observed by transmission electron microscopy (TEM) in ultrathin sections of bacterial cell-containing low speed centrifugation pellets and in partially purified preparations from CsCl equilibrium centrifugation dens...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-5-75

    authors: Chen J,Civerolo EL

    更新日期:2008-06-06 00:00:00

  • Prevalence of various human papillomavirus (HPV) genotypes among women who subjected to routine Pap smear test in Bushehr city (south west of Iran) 2008-2009.

    abstract:BACKGROUND:Some genotypes of human papillomaviruses can infect the genital tract and they are important infectious agents which their oncogenicity is regardable. Thus the aim of this study was to determine the prevalence of various genital human papillomaviruses (HPV) among women being subjected to routine pap smear te...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-65

    authors: Zandi K,Eghbali SS,Hamkar R,Ahmadi S,Ramedani E,Deilami I,Nejad HA,Farshadpour F,Rastian Z

    更新日期:2010-03-22 00:00:00

  • Mouse-adapted scrapie strains 139A and ME7 overcome species barrier to induce experimental scrapie in hamsters and changed their pathogenic features.

    abstract:BACKGROUND:Transmissible spongiform encephalopathy (TSE) diseases are known to be zoonotic diseases that can infect different kinds of animals. The transmissibility of TSE, like that of other infectious diseases, shows marked species barrier, either being unable to infect heterologous species or difficult to form trans...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-63

    authors: Shi Q,Zhang BY,Gao C,Zhang J,Jiang HY,Chen C,Han J,Dong XP

    更新日期:2012-03-09 00:00:00

  • An antisense transcript in the human cytomegalovirus UL87 gene region.

    abstract:BACKGROUND:Rapid advances in research on antisense transcripts are gradually changing our comprehension of genomic and gene expression aspects of the Herpesviridae. One such herpesvirus is the human cytomegalovirus (HCMV). Although transcription of the HCMV UL87 gene has not been specifically investigated, cDNA clones ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-515

    authors: Ma Y,Wang N,Li M,Gao S,Wang L,Ji Y,Qi Y,He R,Sun Z,Ruan Q

    更新日期:2011-11-11 00:00:00

  • The influence of hepatitis B virus on antiviral treatment with interferon and ribavirin in Asian patients with hepatitis C virus/hepatitis B virus coinfection: a meta-analysis.

    abstract:BACKGROUND:Clinical and laboratory studies have indicated that coinfection with hepatitis B virus (HBV) and hepatitis C virus (HCV) can suppress one another, eliciting a dominant disease phenotype. To assess whether HBV can influence the antiviral effect of treatment on HCV, we performed a meta-analysis to comparativel...

    journal_title:Virology journal

    pub_type: 杂志文章,meta分析,评审

    doi:10.1186/1743-422X-9-186

    authors: Liu JY,Sheng YJ,Hu HD,Zhong Q,Wang J,Tong SW,Zhou Z,Zhang DZ,Hu P,Ren H

    更新日期:2012-09-06 00:00:00

  • Comparative genomics of Bacillus thuringiensis phage 0305phi8-36: defining patterns of descent in a novel ancient phage lineage.

    abstract:BACKGROUND:The recently sequenced 218 kb genome of morphologically atypical Bacillus thuringiensis phage 0305phi8-36 exhibited only limited detectable homology to known bacteriophages. The only known relative of this phage is a string of phage-like genes called BtI1 in the chromosome of B. thuringiensis israelensis. Th...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-4-97

    authors: Hardies SC,Thomas JA,Serwer P

    更新日期:2007-10-05 00:00:00

  • Global phylogenetic analysis of contemporary aleutian mink disease viruses (AMDVs).

    abstract:BACKGROUND:Aleutian mink disease has major economic consequences on the mink farming industry worldwide, as it causes a disease that affects both the fur quality and the health and welfare of the mink. The virus causing this disease is a single-stranded DNA virus of the genus Amdoparvovirus belonging to the family of P...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-017-0898-y

    authors: Ryt-Hansen P,Hagberg EE,Chriél M,Struve T,Pedersen AG,Larsen LE,Hjulsager CK

    更新日期:2017-11-22 00:00:00

  • In vitro evaluation of antiviral and virucidal activity of a high molecular weight hyaluronic acid.

    abstract:BACKGROUND:hyaluronic acid (HA), a non-sulphated glycosaminoglycan, is present in synovial fluid, vitreous humour serum and many connective tissues. Pharmaceutical preparations of HA are used in clinical practice for wound healing, joint pain, kerato-conjunctivitis, asthma, mouth care, oesophageal-reflux, and gastritis...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-141

    authors: Cermelli C,Cuoghi A,Scuri M,Bettua C,Neglia RG,Ardizzoni A,Blasi E,Iannitti T,Palmieri B

    更新日期:2011-03-25 00:00:00

  • Identification of a conserved linear B-cell epitope in the M protein of porcine epidemic diarrhea virus.

    abstract:BACKGROUND:The major structural protein of coronaviruses, the membrane (M) protein, can elicit the formation of protective antibodies, but little information is available about the M protein of porcine epidemic diarrhea virus (PEDV). Identification of epitopes on the PEDV M protein will be helpful in the elucidation of...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-225

    authors: Zhang Z,Chen J,Shi H,Chen X,Shi D,Feng L,Yang B

    更新日期:2012-10-01 00:00:00

  • Molecular characterization of HTLV-1 gp46 glycoprotein from health carriers and HAM/TSP infected individuals.

    abstract:BACKGROUND:Human T-cell Leukemia Virus type 1 (HTLV-1) is the etiological agent of tropical spastic paraparesis/HTLV-associated myelopathy (HAM/TSP) that can be identified in around 0.25%-3.8% of the infected population. Disease progression can be monitored by the proviral load and may depend on genetic factors, howeve...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-10-75

    authors: Mota-Miranda AC,Barreto FK,Amarante MF,Batista E,Monteiro-Cunha JP,Farre L,Galvão-Castro B,Alcantara LC

    更新日期:2013-03-06 00:00:00

  • Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells.

    abstract:BACKGROUND:The influenza virus is still one of the most important respiratory risks affecting humans which require effective treatments. In this case, traditional medications are of interest. HESA-A is an active natural biological compound from herbal-marine origin. Previous studies have reported that the therapeutic p...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-44

    authors: Mehrbod P,Ideris A,Omar AR,Hair-Bejo M,Tan SW,Kheiri MT,Tabatabaian M

    更新日期:2012-02-16 00:00:00

  • A mobile genetic element with unknown function found in distantly related viruses.

    abstract:BACKGROUND:The genetic element s2m seems to represent one of very few examples of mobile genetic elements in viruses. The function remains obscure and a scattered taxonomical distribution has been reported by numerous groups. METHODS:We have searched GenBank in order to identify all viral accessions that have s2m(-lik...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-10-132

    authors: Tengs T,Kristoffersen AB,Bachvaroff TR,Jonassen CM

    更新日期:2013-04-25 00:00:00

  • Antibodies against Lewis antigens inhibit the binding of human norovirus GII.4 virus-like particles to saliva but not to intestinal Caco-2 cells.

    abstract:BACKGROUND:Human noroviruses (NoVs) are the main cause of gastroenteritis worldwide. The most commonly detected NoV strains belong to the genetically diverse GII.4 genotype, with new pandemic variants emerging periodically. Despite extensive efforts, NoV investigation has been hampered by the lack of an effective in vi...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-016-0538-y

    authors: Carmona-Vicente N,Allen DJ,Rodríguez-Díaz J,Iturriza-Gómara M,Buesa J

    更新日期:2016-05-21 00:00:00

  • Epidemiologic parameters and evaluation of control measure for 2009 novel influenza a (H1N1) in Xiamen, Fujian Province, China.

    abstract:BACKGROUND:Containment of influenza A H1N1 virus spread was implemented successfully in Xiamen, with large-scale inoculation to reduce morbidity. To identify beneficial elements and to guide decision-making in epidemic containment, we analyzed the epidemiologic parameters and evaluated the control measures. METHOD:We ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-20

    authors: Shen J,Niu J

    更新日期:2012-01-17 00:00:00

  • Highly quantitative serological detection of anti-cytomegalovirus (CMV) antibodies.

    abstract:BACKGROUND:Human cytomegalovirus infection is associated with a variety of pathological conditions including retinitis, pneumonia, hepatitis and encephalitis that may be transmitted congenitally, horizontally and parenterally and occurs both as a primary infection and as reactivation in immunocompromised individuals. C...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-6-45

    authors: Burbelo PD,Issa AT,Ching KH,Exner M,Drew WL,Alter HJ,Iadarola MJ

    更新日期:2009-05-01 00:00:00

  • Vaccination with dengue virus-like particles induces humoral and cellular immune responses in mice.

    abstract:BACKGROUND:The incidence of dengue, an infectious disease caused by dengue virus (DENV), has dramatically increased around the world in recent decades and is becoming a severe public health threat. However, there is currently no specific treatment for dengue fever, and licensed vaccine against dengue is not available. ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-333

    authors: Zhang S,Liang M,Gu W,Li C,Miao F,Wang X,Jin C,Zhang L,Zhang F,Zhang Q,Jiang L,Li M,Li D

    更新日期:2011-06-30 00:00:00

  • Torquetenovirus detection in exosomes enriched vesicles circulating in human plasma samples.

    abstract:BACKGROUND:Torquetenovirus (TTV) belongs to Anelloviridae family, infects nearly all people indefinitely without causing overt disease establishing a fine and successful interaction with the host. Increasing evidence have shown some human viruses exploit extracellular vesicles thereby helping viral persistence in the h...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-018-1055-y

    authors: Martelli F,Macera L,Spezia PG,Medici C,Pistello M,Guasti D,Romagnoli P,Maggi F,Giannecchini S

    更新日期:2018-09-20 00:00:00

  • Capsid display of a conserved human papillomavirus L2 peptide in the adenovirus 5 hexon protein: a candidate prophylactic hpv vaccine approach.

    abstract:BACKGROUND:Infection by any one of 15 high risk human papillomavirus (hrHPV) types causes most invasive cervical cancers. Their oncogenic genome is encapsidated by L1 (major) and L2 (minor) coat proteins. Current HPV prophylactic vaccines are composed of L1 virus-like particles (VLP) that elicit type restricted immunit...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-015-0364-7

    authors: Wu WH,Alkutkar T,Karanam B,Roden RB,Ketner G,Ibeanu OA

    更新日期:2015-09-11 00:00:00