Abstract:
BACKGROUND:Human cytomegalovirus (HCMV) is the most common cause of intrauterine infections worldwide. The toll-like receptors (TLRs) have been reported as important factors in immune response against HCMV. Particularly, TLR2, TLR4 and TLR9 have been shown to be involved in antiviral immunity. Evaluation of the role of single nucleotide polymorphisms (SNPs), located within TLR2, TLR4 and TLR9 genes, in the development of human cytomegalovirus (HCMV) infection in pregnant women and their fetuses and neonates, was performed. METHODS:The study was performed for 131 pregnant women, including 66 patients infected with HCMV during pregnancy, and 65 age-matched control pregnant individuals. The patients were selected to the study, based on serological status of anti-HCMV IgG and IgM antibodies and on the presence of viral DNA in their body fluids. Genotypes in TLR2 2258 A > G, TLR4 896 G > A and 1196 C > T and TLR9 2848 G > A SNPs were determined by self-designed nested PCR-RFLP assays. Randomly selected PCR products, representative for distinct genotypes in TLR SNPs, were confirmed by sequencing. A relationship between the genotypes, alleles, haplotypes and multiple variants in the studied polymorphisms, and the occurrence of HCMV infection in pregnant women and their offsprings, was determined, using a logistic regression model. RESULTS:Genotypes in all the analyzed polymorphisms preserved the Hardy-Weinberg equilibrium in pregnant women, both infected and uninfected with HCMV (P > 0.050). GG homozygotic and GA heterozygotic status in TLR9 2848 G > A SNP decreased significantly the occurrence of HCMV infection (OR 0.44 95% CI 0.21-0.94 in the dominant model, P ≤ 0.050). The G allele in TLR9 SNP was significantly more frequent among the uninfected pregnant women than among the infected ones (χ2 = 4.14, P ≤ 0.050). Considering other polymorphisms, similar frequencies of distinct genotypes, haplotypes and multiple-SNP variants were observed between the studied groups of patients. CONCLUSIONS:TLR9 2848 G > A SNP may be associated with HCMV infection in pregnant women.
journal_name
Virol Jjournal_title
Virology journalauthors
Wujcicka W,Paradowska E,Studzińska M,Wilczyński J,Nowakowska Ddoi
10.1186/s12985-017-0730-8subject
Has Abstractpub_date
2017-03-24 00:00:00pages
64issue
1issn
1743-422Xpii
10.1186/s12985-017-0730-8journal_volume
14pub_type
杂志文章相关文献
Virology Journal文献大全abstract:BACKGROUND:In Europe, the north-south downhill cline frequency of the chemokine receptor CCR5 allele with a 32-bp deletion (CCR5-Delta32) raises interesting questions for evolutionary biologists. We had suggested first that, in the past, the European colonizers, principally Romans, might have been instrumental of a pro...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-119
更新日期:2008-10-16 00:00:00
abstract:BACKGROUND:Cytomegalovirus (CMV) congenital infection is the major viral cause of well-documented birth defects in human. Because CMV is species-specific, the main obstacle to developing animal models for congenital infection is the difference in placental architecture, which preludes virus transmission across the plac...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-3-42
更新日期:2006-06-01 00:00:00
abstract:BACKGROUND:R849 is a neurovirulent γ₁34.5 gene-deficient form of herpes simplex virus type 1 (HSV-1) and has LacZ genes at the deleted sites of the γ₁34.5 gene. HF is a spontaneously occurring, fusogenic HSV-1 strain. The purpose of this work was to generate a virus that has the syncytial character of HF, while preserv...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-294
更新日期:2011-06-10 00:00:00
abstract:BACKGROUND:hyaluronic acid (HA), a non-sulphated glycosaminoglycan, is present in synovial fluid, vitreous humour serum and many connective tissues. Pharmaceutical preparations of HA are used in clinical practice for wound healing, joint pain, kerato-conjunctivitis, asthma, mouth care, oesophageal-reflux, and gastritis...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-141
更新日期:2011-03-25 00:00:00
abstract::Intracellular influenza virus nucleoprotein (NP) is characterized by a high efficiency of homo-polymers formation, however their antigenic structure is still incompletely known. Herein, we report that RNase-resistant intracellular NP homo-polymers have a highly ordered conformational antigenic epitope, which depends o...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-5-37
更新日期:2008-02-29 00:00:00
abstract:BACKGROUND:Influenza A virus evolution in humans is driven at least in part by mutations allowing the virus to escape antibody neutralization. Little is known about the evolution of influenza in birds, a major reservoir of influenza A. METHODS:Neutralizing polyclonal antiserum was raised in chicken against reassortant...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-180
更新日期:2011-04-18 00:00:00
abstract:BACKGROUND:RNA viruses are characterized by high rate of mutations mainly due to the lack of proofreading repair activities associated with its RNA-dependent RNA-polymerase (RdRp). In case of arboviruses, this phenomenon has lead to the existence of mixed population of genomic variants within the host called quasi-spec...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0543-1
更新日期:2016-06-02 00:00:00
abstract:AIM:To investigate the enhancement of humoral immunity when CpG ODN (cytidine phosphate guanosine oligodeoxynucleotides) and aluminium adjuvants are complexed with the HCV (Hepatitis C virus) recombinant immunogen in mice. METHODS:After immunizing Balb/c mice with the recombination HCV antigen adjuvanted with pUCpGs10...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-507
更新日期:2011-11-04 00:00:00
abstract:BACKGROUND:Human noroviruses (NoVs) are the main cause of gastroenteritis worldwide. The most commonly detected NoV strains belong to the genetically diverse GII.4 genotype, with new pandemic variants emerging periodically. Despite extensive efforts, NoV investigation has been hampered by the lack of an effective in vi...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0538-y
更新日期:2016-05-21 00:00:00
abstract:BACKGROUND:Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes acute viral encephalitis in humans. Pigs are important amplifier hosts of JEV. Emerging evidence indicates that host microRNAs (miRNAs) play key roles in modulating viral infection and pathogenesis. However, mechanistic studies deli...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0562-y
更新日期:2016-06-21 00:00:00
abstract:BACKGROUND:Chronic HCV is one of the major causes of morbidity and mortality in the present day world. The assessment of disease progression not only provides useful information for diagnosis and therapeutic supervision judgment but also for monitoring disease. Different invasive and non invasive methods are applied to...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/1743-422X-8-53
更新日期:2011-02-08 00:00:00
abstract:BACKGROUND:Double-stranded RNA (dsRNA) and its mimic, polyinosinic acid: polycytidylic acid [Poly (I:C)], are recognized by toll-like receptor 3 (TLR3) and induce interferon (IFN)-β in many cell types. Poly (I:C) is the most potent IFN inducer. In in vivo mouse studies, intraperitoneal injection of Poly (I:C) elicited ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-114
更新日期:2012-06-14 00:00:00
abstract:BACKGROUND:Hepatitis B virus (HBV) genotype F (HBV/F) is considered to be indigenous to the Americas, but its emergence and spread in the continent remain unknown. Previously, only two HBV/F complete genome sequences from Brazil were available, limiting the contribution of Brazilian isolates to the phylogenetic studies...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-10-236
更新日期:2013-07-16 00:00:00
abstract::In Pakistan, there are estimated 7-9 million carriers of hepatitis B virus (HBV) with a carrier rate of 3-5%. This article reviews the available literature about the prevalence, risk factors, awareness status and genotypes of the HBV in Pakistan by using key words; HBV prevalence, risk factors, awareness status and ge...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/1743-422X-8-102
更新日期:2011-03-06 00:00:00
abstract:BACKGROUND:Although prophylactic vaccines have been developed against HPV6, HPV11, HPV16 and HPV18 there is the clear unmet medical need in order to justify the development of drugs targeting human papillomavirus replication. The native host cells of HPVs are human primary keratinocytes which can be cultivated in raft ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-015-0292-6
更新日期:2015-04-14 00:00:00
abstract:BACKGROUND:Porcine epidemic diarrhea virus (PEDV) is the main causative agent of porcine epidemic diarrhea (PED). Since December 2010, a large-scale outbreak of diarrhea has been observed in swine farms in China. Accumulated evidence indicates that this large-scale outbreak of diarrhea were caused by highly virulent PE...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0529-z
更新日期:2016-04-22 00:00:00
abstract:BACKGROUND:In mammals, interleukin 2 (IL-2) has been shown to decrease replication or attenuate pathogenicity of numerous viral pathogens (herpes simplex virus, vaccinia virus, human respiratory syncytial virus, human immunodeficiency virus) by activating natural killer cells (NK), cytotoxic T lymphocytes and expanding...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-015-0353-x
更新日期:2015-08-08 00:00:00
abstract::Cyclooxygenases (COXs) play a significant role in many different viral infections with respect to replication and pathogenesis. Here we investigated the role of COXs in the mouse hepatitis coronavirus (MHV) infection cycle. Blocking COX activity by different inhibitors or by RNA interference affected MHV infection in ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-4-55
更新日期:2007-06-07 00:00:00
abstract:BACKGROUND:Host serine proteases are essential for the influenza virus life cycle because the viral haemagglutinin is synthesized as a precursor which requires proteolytic maturation. Therefore, we studied the activity and expression of serine proteases in lungs from mice infected with influenza and evaluated the effec...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-27
更新日期:2011-01-20 00:00:00
abstract:BACKGROUND:Foot-and-mouth disease (FMD) is a highly contagious disease of livestock which causes severe economic loss in cloven-hoofed animals. Vaccination is still a major strategy in developing countries to control FMD. Currently, inactivated vaccine of FMDV has been used in many countries with limited success and sa...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-77
更新日期:2011-02-23 00:00:00
abstract::Flaviviruses are small viruses with single-stranded RNA, which include the yellow fever virus, dengue virus, West Nile virus, Japanese encephalitis virus, tick-borne encephalitis virus, and Zika virus; and are causal agents of the most important emerging diseases that have no available treatment to date. In recent yea...
journal_title:Virology journal
pub_type: 杂志文章,评审
doi:10.1186/s12985-017-0761-1
更新日期:2017-05-15 00:00:00
abstract:BACKGROUND:HCV is prevalent throughout the world. It is a major cause of chronic liver disease. There is no effective vaccine and the most common therapy, based on Peginterferon, has a success rate of ~50%. The mechanisms underlying viral resistance have not been elucidated but it has been suggested that both host and ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-14
更新日期:2012-01-12 00:00:00
abstract::Papillomaviruses (PVs) are associated with the development of neoplasias and have been found in several different species, most of them in humans and other mammals. We identified, cloned and sequenced PV DNA from pigmented papilloma-like lesions of a diamond python (Morelia spilota spilota). This represents the first ...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-436
更新日期:2011-09-12 00:00:00
abstract:BACKGROUND:Little is known at the molecular level concerning the differences and/or similarities between alcohol and hepatitis C virus induced liver disease. Global transcriptional profiling using oligonucleotide microarrays was therefore performed on liver biopsies from patients with cirrhosis caused by either chronic...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-3-98
更新日期:2006-11-22 00:00:00
abstract:BACKGROUND:A novel reassortant H7N9 influenza A virus has crossed the species barrier from poultry to cause human infections in China in 2013 and 2014. Rapid detection of the novel H7N9 virus is important to detect this virus in poultry and reduce the risk of an epidemic in birds or humans. FINDINGS:In this study, a m...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-015-0300-x
更新日期:2015-04-30 00:00:00
abstract:BACKGROUND:Human cytomegalovirus infection is associated with a variety of pathological conditions including retinitis, pneumonia, hepatitis and encephalitis that may be transmitted congenitally, horizontally and parenterally and occurs both as a primary infection and as reactivation in immunocompromised individuals. C...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-6-45
更新日期:2009-05-01 00:00:00
abstract:BACKGROUND:Influenza A virus poses a continuous threat to global public health. Design of novel universal drugs and vaccine requires a careful analysis of different strains of Influenza A viral genome from diverse hosts and subtypes. We performed a systematic in silico analysis of Influenza A viral segments of all avai...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-44
更新日期:2011-01-27 00:00:00
abstract:BACKGROUND:West Nile virus (WNV) is an emerging zoonotic pathogen which is harmful to human and animal health. Effective vaccination in susceptible hosts should protect against WNV infection and significantly reduce viral transmission between animals and from animals to humans. A versatile vaccine suitable for differen...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/s12985-016-0568-5
更新日期:2016-06-24 00:00:00
abstract:BACKGROUND:Bovine viral diarrhea virus (BVDV) infections continue to cause significantly losses in the deer population. Better isolation and identification of BVDV from sika deer may contribute significantly to the development of prophylactic therapeutic, and diagnostic reagents as well as help in prevention and contro...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-8-83
更新日期:2011-02-25 00:00:00
abstract:BACKGROUND:The influenza virus is still one of the most important respiratory risks affecting humans which require effective treatments. In this case, traditional medications are of interest. HESA-A is an active natural biological compound from herbal-marine origin. Previous studies have reported that the therapeutic p...
journal_title:Virology journal
pub_type: 杂志文章
doi:10.1186/1743-422X-9-44
更新日期:2012-02-16 00:00:00