Attenuation of influenza virus infectivity with herbal-marine compound (HESA-A): an in vitro study in MDCK cells.

Abstract:

BACKGROUND:The influenza virus is still one of the most important respiratory risks affecting humans which require effective treatments. In this case, traditional medications are of interest. HESA-A is an active natural biological compound from herbal-marine origin. Previous studies have reported that the therapeutic properties of HESA-A are able to treat psoriasis vulgaris and cancers. However, no antiviral properties have been reported. METHODS:This study was designed to investigate the potential antiviral properties of HESA-A and its effects in modulating TNF-α and IL-6 cytokine levels. HESA-A was prepared in normal saline as a stock solution (0.8 mg/ml, pH = 7.4). Percentages of cell survival when exposed to different concentrations of HESA-A at different time intervals was determined by MTT assay. To study the potential antiviral activity of HESA-A, Madin-Darby Canine Kidney (MDCK) cells were treated with the effective concentration (EC50) of HESA-A (0.025 mg/ml) and 100 TCID50/0.1 ml of virus sample under different types of exposure. RESULTS:Based on the MTT method and hemagglutination assay (HA), HESA-A is capable of improving cell viability to 31% and decreasing HA titre to almost 99% in co-penetration exposures. In addition, based on quantitative real-time PCR (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA), it was found that HESA-A causes decrements in TNF-α and IL-6 cytokine expressions, which was significant for TNF-α (p ≤ 0.05) but not for IL-6. CONCLUSION:In conclusion, HESA-A was effective against influenza infection through suppressing cytokine expression.

journal_name

Virol J

journal_title

Virology journal

authors

Mehrbod P,Ideris A,Omar AR,Hair-Bejo M,Tan SW,Kheiri MT,Tabatabaian M

doi

10.1186/1743-422X-9-44

subject

Has Abstract

pub_date

2012-02-16 00:00:00

pages

44

issn

1743-422X

pii

1743-422X-9-44

journal_volume

9

pub_type

杂志文章
  • Highly quantitative serological detection of anti-cytomegalovirus (CMV) antibodies.

    abstract:BACKGROUND:Human cytomegalovirus infection is associated with a variety of pathological conditions including retinitis, pneumonia, hepatitis and encephalitis that may be transmitted congenitally, horizontally and parenterally and occurs both as a primary infection and as reactivation in immunocompromised individuals. C...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-6-45

    authors: Burbelo PD,Issa AT,Ching KH,Exner M,Drew WL,Alter HJ,Iadarola MJ

    更新日期:2009-05-01 00:00:00

  • The duck hepatitis virus 5'-UTR possesses HCV-like IRES activity that is independent of eIF4F complex and modulated by downstream coding sequences.

    abstract::Duck hepatitis virus (DHV-1) is a worldwide distributed picornavirus that causes acute and fatal disease in young ducklings. Recently, the complete genome of DHV-1 has been determined and comparative sequence analysis has shown that possesses the typical picornavirus organization but exhibits several unique features. ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-147

    authors: Liu G,Yángüez E,Chen Z,Li C

    更新日期:2011-03-31 00:00:00

  • Development and validation of a prokaryotically expressed foot-and-mouth disease virus non-structural protein 2C'3AB-based immunochromatographic strip to differentiate between infected and vaccinated animals.

    abstract:BACKGROUND:Foot-and-mouth disease (FMD) is an extremely contagious viral disease of cattle, pigs, sheep, goats, and many cloven-hoofed wild animals. FMDV serotypes O and Asia 1 have circulated separately in China during the last fifty years, and eliminating infected animals and vaccination are the main policies to prev...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-186

    authors: Wu L,Jiang T,Lu ZJ,Yang YM,Sun P,Liang Z,Li D,Fu YF,Cao YM,Liu XT,Liu ZX

    更新日期:2011-04-23 00:00:00

  • Difference in cytokine production and cell cycle progression induced by Epstein-Barr virus Lmp1 deletion variants in Kmh2, a Hodgkin lymphoma cell line.

    abstract:BACKGROUND:Epstein-Barr virus (EBV) is associated with 20-40% of Hodgkin's Lymphoma (HL) cases. EBV-encoded latent membrane protein 1 (LMP1) is a well-known oncogenic protein and two C-terminal deletion variants, del30-LMP1 and del69-LMP1, have been described in animal models to be more tumorigenic than the wild-type f...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-11-94

    authors: Sueur C,Lupo J,Mas P,Morand P,Boyer V

    更新日期:2014-05-19 00:00:00

  • Evidence of recombination in quasispecies populations of a Hepatitis C Virus patient undergoing anti-viral therapy.

    abstract:BACKGROUND/AIM:Hepatitis C virus (HCV) has been the subject of intense research and clinical investigation as its major role in human disease has emerged. HCV circulates in vivo as a complex population of different but closely related viral variants, commonly referred to as a quasispecies. The extent to which recombina...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-3-87

    authors: Moreno MP,Casane D,López L,Cristina J

    更新日期:2006-10-24 00:00:00

  • A small molecule inhibitor of dengue virus type 2 protease inhibits the replication of all four dengue virus serotypes in cell culture.

    abstract:BACKGROUND:Dengue has emerged as the most significant of arboviral diseases in the 21st century. It is endemic to >100 tropical and sub-tropical countries around the world placing an estimated 3.6 billion people at risk. It is caused by four genetically similar but antigenically distinct, serotypes of dengue viruses. T...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-015-0248-x

    authors: Raut R,Beesetti H,Tyagi P,Khanna I,Jain SK,Jeankumar VU,Yogeeswari P,Sriram D,Swaminathan S

    更新日期:2015-02-08 00:00:00

  • Reduction of the survival time of pig xenotransplants by porcine cytomegalovirus.

    abstract:BACKGROUND:Xenotransplantation using pig cells, tissues and organs may help to overcome the shortage of human tissues and organs for the treatment of tissue and organ failure. Progress in the prevention of immunological rejection using genetically modified pigs and new, more effective, immunosuppression regimens will a...

    journal_title:Virology journal

    pub_type: 杂志文章,评审

    doi:10.1186/s12985-018-1088-2

    authors: Denner J

    更新日期:2018-11-08 00:00:00

  • Role of human Pegivirus infections in whole Plasmodium falciparum sporozoite vaccination and controlled human malaria infection in African volunteers.

    abstract:BACKGROUND:Diverse vaccination outcomes and protection levels among different populations pose a serious challenge to the development of an effective malaria vaccine. Co-infections are among many factors associated with immune dysfunction and sub-optimal vaccination outcomes. Chronic, asymptomatic viral infections can ...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-021-01500-8

    authors: Tumbo AM,Schindler T,Dangy JP,Orlova-Fink N,Bieri JR,Mpina M,Milando FA,Juma O,Hamad A,Nyakarungu E,Chemba M,Mtoro A,Ramadhan K,Olotu A,Makweba D,Mgaya S,Stuart K,Perreau M,Stapleton JT,Jongo S,Hoffman SL,Tanner

    更新日期:2021-01-26 00:00:00

  • Duck enteritis virus UL54 is an IE protein primarily located in the nucleus.

    abstract:BACKGROUND:The UL54 protein of Duck Enteritis Virus (DEV) is a homolog of herpes simplex virus-1 (HSV-1) immediate-early infectious cell protein 27 (ICP27), a multifunctional protein essential for viral infection. Nonetheless, there is little information on the UL54 protein of DEV. METHODS:The UL54 gene was cloned int...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-015-0424-z

    authors: Liu C,Cheng A,Wang M,Chen S,Jia R,Zhu D,Liu M,Sun K,Yang Q,Chen X

    更新日期:2015-11-25 00:00:00

  • Identification of a conserved linear B-cell epitope in the M protein of porcine epidemic diarrhea virus.

    abstract:BACKGROUND:The major structural protein of coronaviruses, the membrane (M) protein, can elicit the formation of protective antibodies, but little information is available about the M protein of porcine epidemic diarrhea virus (PEDV). Identification of epitopes on the PEDV M protein will be helpful in the elucidation of...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-225

    authors: Zhang Z,Chen J,Shi H,Chen X,Shi D,Feng L,Yang B

    更新日期:2012-10-01 00:00:00

  • Inhibition of HIV early replication by the p53 and its downstream gene p21.

    abstract:BACKGROUND:The tumor suppressor gene p53 has been found to suppress HIV infection by various mechanisms, but the inhibition of HIV at an early stage of replication by host cell p53 and its downstream gene p21 has not been well studied. METHOD:VSV-G pseudotyped HIV-1 or HIV-2 viruses with GFP or luciferase reporter gen...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-018-0959-x

    authors: Shi B,Sharifi HJ,DiGrigoli S,Kinnetz M,Mellon K,Hu W,de Noronha CMC

    更新日期:2018-03-27 00:00:00

  • Identification of a novel canine parvovirus type 2c in Taiwan.

    abstract:BACKGROUND:Taiwan has been considered free from canine parvovirus type 2c (CPV-2c) based on the last report of canine parvovirus type 2 (CPV-2) surveillance. However, since January 2015, the first report of CPV-2c in a puppy has occurred in Taiwan. There is currently limited information about the CPV-2c variant in Taiw...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-016-0620-5

    authors: Chiang SY,Wu HY,Chiou MT,Chang MC,Lin CN

    更新日期:2016-09-23 00:00:00

  • Multiple organ involvement in severe fever with thrombocytopenia syndrome: an immunohistochemical finding in a fatal case.

    abstract:BACKGROUND:Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by SFTS bunyavirus (SFTSV), a tick borne bunyavirus. However, Immunohistochemistry of SFTS patients are not well studied. METHODS:We obtained multiple of tissues from a fatal case with SFTS, including blood, lungs, k...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-018-1006-7

    authors: Li S,Li Y,Wang Q,Yu X,Liu M,Xie H,Qian L,Ye L,Yang Z,Zhang J,Zhu H,Zhang W

    更新日期:2018-05-30 00:00:00

  • Complete genome sequence of human astrovirus genotype 6.

    abstract:BACKGROUND:Human astroviruses (HAstVs) are one of the important causes of acute gastroenteritis in children. Currently, eight HAstV genotypes have been identified and all but two (HAstV-6 and HAstV-7) have been fully sequenced. We here sequenced and analyzed the complete genome of a HAstV-6 strain (192-BJ07), which was...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-29

    authors: Guo L,Gonzalez R,Wang W,Li Y,Paranhos-Baccalà G,Vernet G,Wang J

    更新日期:2010-02-08 00:00:00

  • Neutralization sensitivity of HIV-1 subtype B' clinical isolates from former plasma donors in China.

    abstract:BACKGROUND:HIV-1 subtype B' isolates have been predominantly circulating in China. Their intra- and inter-subtype neutralization sensitivity to autologous and heterologous plasmas has not been well studied. RESULTS:Twelve HIV-1 B' clinical isolates obtained from patients were tested for their intra- and inter-subtype ...

    journal_title:Virology journal

    pub_type: 杂志文章,多中心研究

    doi:10.1186/1743-422X-10-10

    authors: OuYang Y,Sun J,Huang Y,Lu L,Xu W,Hu X,Hong K,Jiang S,Shao Y,Ma L

    更新日期:2013-01-05 00:00:00

  • Co-circulation of multiple reassortant H6 subtype avian influenza viruses in wild birds in eastern China, 2016-2017.

    abstract:BACKGROUND:H6 subtype influenza viruses were prevalent in domestic poultry and wild birds, which also could pose potential threat to humans. However, little is known about the prevalence of H6 subtype viruses in wild birds in eastern China, a crucial stopover or wintering site for migratory wild birds along the East As...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-020-01331-z

    authors: Hu C,Li X,Zhu C,Zhou F,Tang W,Wu D,Li Z,Zhou L,Liu J,Wei X,Cui J,Wang T,He G

    更新日期:2020-04-29 00:00:00

  • Vaccination of ferrets with a recombinant G glycoprotein subunit vaccine provides protection against Nipah virus disease for over 12 months.

    abstract:BACKGROUND:Nipah virus (NiV) is a zoonotic virus belonging to the henipavirus genus in the family Paramyxoviridae. Since NiV was first identified in 1999, outbreaks have continued to occur in humans in Bangladesh and India on an almost annual basis with case fatality rates reported between 40% and 100%. METHODS:Ferret...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-10-237

    authors: Pallister JA,Klein R,Arkinstall R,Haining J,Long F,White JR,Payne J,Feng YR,Wang LF,Broder CC,Middleton D

    更新日期:2013-07-16 00:00:00

  • Seroprevalence of human respiratory syncytial virus and human metapneumovirus in healthy population analyzed by recombinant fusion protein-based enzyme linked immunosorbent assay.

    abstract:BACKGROUND:Human respiratory syncytial virus (hRSV) and human metapneumovirus (hMPV) are two of the most frequent respiratory pathogens that circulate worldwide. Infection with either virus can lead to hospitalization of young children, immunocompromised people and the elderly.A better understanding of the epidemiologi...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-130

    authors: Sastre P,Ruiz T,Schildgen O,Schildgen V,Vela C,Rueda P

    更新日期:2012-07-02 00:00:00

  • Early detection of Varicella-Zoster virus (VZV)-specific T-cells before seroconversion in primary varicella infection: case report.

    abstract::Here we report the case of a 54-year old, immunocompetent German patient with primary varicella whose Varicella-Zoster Virus (VZV)-specific T-cell responses could be detected early in infection and before the onset of seroconversion. This case demonstrates that the detection of VZV-specific T-cells may under certain c...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-7-54

    authors: Baiker A,Haase R,Eberle J,Vizoso Pinto MG,Pfrepper KI,Petrich A,Deml L,Campe H,Nitschko H,Jaeger G

    更新日期:2010-03-06 00:00:00

  • Inhibition effects of patchouli alcohol against influenza a virus through targeting cellular PI3K/Akt and ERK/MAPK signaling pathways.

    abstract:BACKGROUND:Patchouli alcohol (PA) is a tricyclic sesquiterpene extracted from Pogostemonis Herba, which is a traditional Chinese medicine used for therapy of inflammatory diseases. Recent studies have shown that PA has various pharmacological activities, including anti-bacterial and anti-viral effects. METHODS:In this...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-019-1266-x

    authors: Yu Y,Zhang Y,Wang S,Liu W,Hao C,Wang W

    更新日期:2019-12-23 00:00:00

  • The altered gut virome community in rhesus monkeys is correlated with the gut bacterial microbiome and associated metabolites.

    abstract:BACKGROUND:The gut microbiome is closely associated with the health of the host; although the interaction between the bacterial microbiome and the whole virome has rarely been studied, it is likely of medical importance. Examination of the interactions between the gut bacterial microbiome and virome of rhesus monkey wo...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-019-1211-z

    authors: Li H,Li H,Wang J,Guo L,Fan H,Zheng H,Yang Z,Huang X,Chu M,Yang F,He Z,Li N,Yang J,Wu Q,Shi H,Liu L

    更新日期:2019-08-19 00:00:00

  • Hepatitis B virus in Pakistan: a systematic review of prevalence, risk factors, awareness status and genotypes.

    abstract::In Pakistan, there are estimated 7-9 million carriers of hepatitis B virus (HBV) with a carrier rate of 3-5%. This article reviews the available literature about the prevalence, risk factors, awareness status and genotypes of the HBV in Pakistan by using key words; HBV prevalence, risk factors, awareness status and ge...

    journal_title:Virology journal

    pub_type: 杂志文章,评审

    doi:10.1186/1743-422X-8-102

    authors: Ali M,Idrees M,Ali L,Hussain A,Ur Rehman I,Saleem S,Afzal S,Butt S

    更新日期:2011-03-06 00:00:00

  • Phylogenetic exploration of hantaviruses in Paraguay reveals reassortment and host switching in South America.

    abstract:BACKGROUND:Longitudinal mark-recapture studies of rodents in two sites in the Mbaracayú Biosphere Reserve in the Interior Atlantic Forest of eastern Paraguay have revealed a complex and intriguing pattern of hantaviruses harbored by rodents in this area. Full-length sequencing and phylogenetic analyses were conducted f...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-399

    authors: Chu YK,Owen RD,Jonsson CB

    更新日期:2011-08-12 00:00:00

  • Epidemiology and clinical features of rotavirus and norovirus infection among children in Ji'nan, China.

    abstract:BACKGROUND:Acute gastroenteritis caused by bacteria, virus and parasite is an important cause of childhood morbidity and mortality in developing countries. Rotavirus and norovirus have been recognized as the most common pathogens causing acute gastroenteritis among children. However, there is still no valuable data abo...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-10-302

    authors: Sai L,Sun J,Shao L,Chen S,Liu H,Ma L

    更新日期:2013-10-08 00:00:00

  • A novel ViewRNA in situ hybridization method for the detection of the dynamic distribution of Classical Swine Fever Virus RNA in PK15 cells.

    abstract:BACKGROUND:Classical swine fever (CSF) is a highly contagious fatal infectious disease caused by classical swine fever virus (CSFV). A better understanding of CSFV replication is important for the study of pathogenic mechanism of CSF. With the development of novel RNA in situ Hybridization method, quantitatively locali...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-017-0734-4

    authors: Zhang Q,Xu L,Zhang Y,Wang T,Zou X,Zhu Y,Zhao Y,Li C,Chen K,Sun Y,Sun J,Zhao Q,Wang Q

    更新日期:2017-04-18 00:00:00

  • Phylogenetic analysis of Torque Teno Virus genome from Pakistani isolate and incidence of co-infection among HBV/HCV infected patients.

    abstract:BACKGROUND:Torque Teno Virus (TTV) was the first single stranded circular DNA virus to be discovered that infects humans. Although there have been numerous reports regarding the prevalence of TTV from other countries of South Asia, there is severe lack of information regarding its prevalence in Pakistan. Thus the prese...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-9-320

    authors: Hussain T,Manzoor S,Waheed Y,Tariq H,Hanif K

    更新日期:2012-12-28 00:00:00

  • Vomiting and wasting disease associated with hemagglutinating encephalomyelitis viruses infection in piglets in Jilin, China.

    abstract::One coronavirus strain was isolated from brain tissues of ten piglets with evident clinical manifestations of vomiting, diarrhea and dyskinesia in Jilin province in China. Antigenic and genomic characterizations of the virus (isolate PHEV-JLsp09) were based on multiplex PCR and negative staining electron microscopy an...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-8-130

    authors: Gao W,Zhao K,Zhao C,Du C,Ren W,Song D,Lu H,Chen K,Li Z,Lan Y,Xie S,He W,Gao F

    更新日期:2011-03-21 00:00:00

  • Favipiravir versus other antiviral or standard of care for COVID-19 treatment: a rapid systematic review and meta-analysis.

    abstract:BACKGROUND:The COVID-19 causing coronavirus is an enveloped RNA virus that utilizes an enzyme RNA dependent RNA polymerase for its replication. Favipiravir (FVP) triphosphate, a purine nucleoside analog, inhibits that enzyme. We have conducted this systematic review and meta-analysis on efficacy and safety of the drug ...

    journal_title:Virology journal

    pub_type: 杂志文章,meta分析

    doi:10.1186/s12985-020-01412-z

    authors: Shrestha DB,Budhathoki P,Khadka S,Shah PB,Pokharel N,Rashmi P

    更新日期:2020-09-24 00:00:00

  • Development of one-step quantitative reverse transcription PCR for the rapid detection of flaviviruses.

    abstract:BACKGROUND:The genus Flavivirus includes several pathogenic agents that cause severe illness in humans. Re-emergence of West Nile virus in Europe and continuous spread of certain flaviviruses such as dengue, yellow fever and Japanese encephalitis viruses represent a global danger to public health. Therefore, a rapid an...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/1743-422X-10-58

    authors: Patel P,Landt O,Kaiser M,Faye O,Koppe T,Lass U,Sall AA,Niedrig M

    更新日期:2013-02-14 00:00:00

  • Specific and quantitative detection of Human polyomaviruses BKPyV and JCPyV in the healthy Pakistani population.

    abstract:BACKGROUND:The BK Polyomavirus (BKPyV) and JC polyomavirus (JCPyV) infections are widespread in human population and have been associated with severe kidney and brain disorders, respectively. The viruses remain latent primarily in reno-urinary tract, reactivating only in case of a compromised immune system. The seroepi...

    journal_title:Virology journal

    pub_type: 杂志文章

    doi:10.1186/s12985-017-0752-2

    authors: Hussain I,Tasneem F,Umer M,Pervaiz A,Raza M,Arshad MI,Shahzad N

    更新日期:2017-04-24 00:00:00