Abstract:
:We describe an improvement of an earlier reported real-time RT-PCR assay for the detection of enterovirus RNA, based on the 5' exonuclease digestion of a dual-labeled fluorogenic probe by Taq DNA polymerase. A different extraction method, real-time RT-PCR instrument and primer set were evaluated. Our data show that the optimized assay yields a higher sensitivity and reproducibility and resulted in a significant reduced hands-on time per sample.
journal_name
Virol Jjournal_title
Virology journalauthors
Piqueur MA,Verstrepen WA,Bruynseels P,Mertens AHdoi
10.1186/1743-422X-6-95subject
Has Abstractpub_date
2009-07-07 00:00:00pages
95issn
1743-422Xpii
1743-422X-6-95journal_volume
6pub_type
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