当前位置： SCI文献检索 > JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION期刊下所有文献 > Solubilized TSH-receptor: its usefulness for the radioligand receptor assay for TSH and TSH-displacing antibody.

Solubilized TSH-receptor: its usefulness for the radioligand receptor assay for TSH and TSH-displacing antibody.

Abstract：

:The usefulness of solubilized TSH-receptor for measuring TSH and TSH-displacing antibodies (TDA) in a radioligand receptor assay (RRA) was investigated. Crude thyroid membranes were solubilized with Triton X-100. A soluble protein fraction was isolated by DEAE-Sephadex A-50 ion-exchange chromatography. The binding of 125I-TSH to the solubilized fraction was studied. It was maximal after 45 min at 37 C, whereas at 4 C a similar binding was observed after at least 17 h. The binding was inhibited by Ca2+ and Mg2+ with concentrations higher than 1 mM and by NaCl in the concentration range 17-150 mM. No inhibition of binding was found with normal serum up to 100 microliter. In contrast, 1.5 microliter of a Graves' disease serum showed significant inhibition. Using the system to measure TSH in serum, as low as 10 microunits TSH could be estimated. Compared to an RRA using thyroid membrane fractions, the sensitivity for TSH in serum as well as for TDA is increased at least tenfold in our system.

journal_name

J Endocrinol Invest

journal_title

Journal of endocrinological investigation

authors

Kotulla P,Schleusener H

doi

10.1007/BF03350444

subject

Has Abstract

pub_date

1981-04-01 00:00:00

pages

155-60

issue

2

eissn

0391-4097

issn

1720-8386

journal_volume

4

pub_type

杂志文章

相关文献

JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION文献大全
  • Ovarian steroids modulate neuroendocrine dysfunction in polycystic ovary syndrome.

    abstract：OBJECTIVE:Neuroendocrine dysfunction in polycystic ovary syndrome (PCOS) was addressed by studying the steroid hormone changes in women with PCOS with either high or normal LH levels leading to inferences regarding the primacy of elevated LH in the pathophysiology of PCOS. METHODS:A cross-sectional study was designed ...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03345319

    authors： Doi SA,Al-Zaid M,Towers PA,Scott CJ,Al-Shoumer KA

    更新日期：2005-11-01 00:00:00

  • Spontaneous regression of hypercalcemia in a patient with primary hyperparathyroidism and prolactinoma.

    abstract：:We describe a unique case of spontaneous resolution of hyperparathyroidism in a lady with combined parathyroid adenoma and prolactinoma, raising the possibility of underlying multiple endocrine neoplasia (MEN) 1 syndrome. We also discuss the mechanism and natural history of such spontaneous remission. ...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03345292

    authors： Baskar V,Kamalakannan D,Singh BM,Odum J

    更新日期：2004-05-01 00:00:00

  • Testosterone therapy in men: clinical and pharmacological perspectives.

    abstract：:There is increasing evidence that androgen therapy in men may be effectively applied in several conditions to improve well being and health. Classical indications for androgen therapy in males are represented by primary or secondary hypogonadism, delayed puberty, aplastic anemia and that secondary to chronic renal fai...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,评审

    doi：10.1007/BF03343707

    authors： Gambineri A,Pasquali R

    更新日期：2000-03-01 00:00:00

  • Endocannabinoids and energy metabolism.

    abstract：:Although adjustments to nutritional lifestyle and increased physical activity remain the milestones of weight loss therapy, it is evident from the exponential increase of the number of obese subjects in Western countries that these two approaches alone are no longer able to limit this progression. This alarming phenom...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,评审

    doi：

    authors： Pagotto U,Pasquali R

    更新日期：2006-01-01 00:00:00

  • Study of the family of a patient with male-limited precocious puberty (MPP) due to T1193C transition in exon 11 of LH receptor gene.

    abstract：:Molecular diagnostics of the LHR gene was conducted in a 5-year-old boy with clinical symptoms and hormonal profile typical of precocious puberty. His parents and 4 sisters were also diagnosed. Single-strand conformation polymorphism analysis under temperature gradient conditions (Multitemperature SSCP) of 3 overlappi...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03344001

    authors： Ignacak M,Starzyk J,Dziatkowiak H,Trzeciak WH

    更新日期：2002-03-01 00:00:00

  • Hyperthyroidism due to familial pituitary resistance to thyroid hormone: successful control with 3, 5, 3' triiodothyroacetic associated to propranolol.

    abstract：:We herein describe a family with thyroid hormone resistance. Thyroid hormones and basal TSH were elevated. Pituitary tumor or abnormality in thyroid hormone binding proteins were ruled out by appropriate tests. Mother and sister of the propositus presented similar abnormal hormonal features but no hyperthyroidism. Ini...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03347890

    authors： Aguilar Diosdado M,Escobar-Jimenez L,Fernandez Soto ML,Garcia Curiel A,Escobar-Jimenez F

    更新日期：1991-09-01 00:00:00

  • Growth hormone insensitivity: diagnostic and therapeutic approaches.

    abstract：INTRODUCTION:Growth hormone resistance defines several genetic (primary) and acquired (secondary) pathologies that result in completely or partially interrupted activity of growth hormone. An archetypal disease of this group is the Laron-type dwarfism caused by mutations in growth hormone receptors. The diagnosis is ba...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,评审

    doi：10.1007/s40618-015-0327-2

    authors： Kurtoğlu S,Hatipoglu N

    更新日期：2016-01-01 00:00:00

  • Comparison of circulating and local adipose tissue renin-angiotensin system in normotensive and hypertensive obese subjects.

    abstract：:The renin-angiotensin-aldosterone system (RAAS) plays a well-recognized role in the regulation of BP and in salt and water balance. Since hypertension affects a considerable proportion of obese patients, circulating RAAS has been studied in obese subjects with and without hypertension, albeit with conflicting results....

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03344010

    authors： Faloia E,Gatti C,Camilloni MA,Mariniello B,Sardu C,Garrapa GG,Mantero F,Giacchetti G

    更新日期：2002-04-01 00:00:00

  • Clinical and endocrine features and long-term outcome of Graves' disease in early childhood.

    abstract：:Hyperthyroidism is rare in early childhood and most commonly caused by Graves' disease. We report 14 children (4 boys, 10 girls) aged 3.4-7.5 yr. At diagnosis, all patients had weight loss, hyperkinetic activity, tachycardia, difficulty sleeping, and poor concentration and 11 presented with proptosis. Four patients de...

    journal_title：Journal of endocrinological investigation

    pub_type： 临床试验,杂志文章

    doi：10.1007/BF03346315

    authors： Bossowski AT,Reddy V,Perry LA,Johnston LB,Banerjee K,Blair JC,Savage MO

    更新日期：2007-05-01 00:00:00

  • Decreased IGF-I bioavailability after ethanol abuse in alcoholics: partial restitution after short-term abstinence.

    abstract：:IGF-I stimulates protein synthesis, lowers blood glucose, and affects cell differentiation. The main production site of IGF-I is the liver. One of its binding proteins, IGFBP-1, is also produced by the liver. It is well known that ethanol affects the function of the human liver. Long-term alcohol abuse may therefore n...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03343879

    authors： Röjdmark S,Brismar K

    更新日期：2001-07-01 00:00:00

  • Bone mineral density and body composition in male children with hypogonadism.

    abstract：:Estrogen deficiency in females and androgen deficiency in males may harm periosteal and endosteal apposition, reduce bone size and both cortical and trabecular thickness, modifying in this way the bone structure later in life. To date, few systematic studies on bone mineral density (BMD) and hypogonadism in adolescent...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03346513

    authors： Fintini D,Grossi A,Brufani C,Fiori R,Ubertini G,Pecorelli L,Cappa M

    更新日期：2009-07-01 00:00:00

  • The growth hormone response to hexarelin in patients with Prader-Willi syndrome.

    abstract：:Hexarelin (Hex) is a synthetic hexapeptide with potent GH-releasing activity in both animals and men. Aim of this study was to evaluate the GH response to a maximal dose of Hex and GH-releasing hormone (GHRH) in a group of patients with Prader-Willi syndrome (PWS). Seven patients (4 boys and 3 girls, age 2.4-14.2 yr) ...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03347335

    authors： Cappa M,Raguso G,Palmiotto T,Faedda A,Gurreri F,Neri G,Deghenghi R,Loche S

    更新日期：1998-09-01 00:00:00

  • Androgen producing adrenal adenoma. Report on a case associated with hyperparathyroidism.

    abstract：:In a 41-year-old hirsute woman, severe hypercalcemia led to the discovery of hyperparathyroidism related to the involvement (hyperplasia/or adenoma) of the 4 parathyroid glands. Plasma and urinary DHA, plasma DHA-sulfate and delta 5 steroid precursors were elevated. Steroid hormone hypersecretion was stimulated by hCG...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03347005

    authors： Guillausseau PJ,Boitard C,Le Charpentier Y,Cedard L,Nahoul K,Blacker C,Kaloustian E,Courtalhac-Kaloustian F,Dubost C,Lubetzki J

    更新日期：1987-12-01 00:00:00

  • Improved anti-apoptotic and anti-remodeling potency of bone marrow mesenchymal stem cells by anoxic pre-conditioning in diabetic cardiomyopathy.

    abstract：BACKGROUND:Bone marrow mesenchymal stem cells (MSC) mediate their protection by paracrine mechanism under ischemic conditions and anoxic pre-conditioning (AP) of MSC strongly enhances their survival and regenerative capacity. However, there is no report about the therapeutic potency of MSC transplantation on diabetic c...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03345575

    authors： Li JH,Zhang N,Wang JA

    更新日期：2008-02-01 00:00:00

  • The effects of calcitonin on idiopathic nephrolithiasis. Evidence of bone involvement in fasting hypercalciuria.

    abstract：:This study was performed to evaluate the antihypercalciuric effect of calcitonin (CT), a potent inhibitor of bone osteoclastic activity, on idiopathic hypercalciuria (IH). Forty-two stone formers were studied: 18 suffered from fasting hypercalciuria (FH), 12 from nonfasting hypercalciuria (NFH) and 12 were normocalciu...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03350171

    authors： Filipponi P,Mannarelli C,Gubbiotti G,Blass A,Moretti I,Tini S,Giuseppetti N,Ballanti S,Morucci P

    更新日期：1988-07-01 00:00:00

  • The role of placental iodine storage in the neonatal thyroid stimulating hormone surge: iodine as a driving force to adapt the terrestrial life.

    abstract：PURPOSE:Iodine plays a pivotal role in adaptation during the transition from intrauterine to extrauterine life. Although it is well known that the placenta plays a role in iodine storage, a relationship between the neonatal thyroid stimulating hormone (TSH) peak and placental iodine concentration has not been establish...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/s40618-020-01399-y

    authors： Karaoglan M,İşbilen E

    更新日期：2020-08-28 00:00:00

  • Lymphocytic hypophysitis: report of an unusual case of a rare disorder.

    abstract：:A 36-year-old non-pregnant woman presented with a four-month history of progressive visual deterioration and amenorrhea. The latest gestation was 6 years earlier. Hormonal study revealed central diabetes insipidus, hypopituitarism, and slightly increased prolactin level. Ophthalmologic examination showed bilateral hem...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03343842

    authors： Durán Martínez M,Santonja C,Pavón de Paz I,Monereo Megías S

    更新日期：2001-03-01 00:00:00

  • Pituitary imaging abnormalities in patients with and without hypopituitarism after traumatic brain injury.

    abstract：:Recent evidence suggests that patients with traumatic brain injury (TBI) are at substantial risk of hypopituitarism. The pathomechanisms, however, are not completely understood yet. Little is known about the association of morphological changes in the sella region with pituitary function in TBI. In this study, we asse...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03346291

    authors： Schneider HJ,Sämann PG,Schneider M,Croce CG,Corneli G,Sievers C,Ghigo E,Stalla GK,Aimaretti G

    更新日期：2007-04-01 00:00:00

  • Interrelationships between follicle stimulating hormone and the growth hormone--insulin-like growth factor--IGF-binding proteins axes in human granulosa cells in culture.

    abstract：:As it has been hypothesized that IGF-binding proteins (IGFBPs) may have a role as autocrine/paracrine factors in regulating the local actions of the insulin-like growth factors (IGFs) in the ovary, we studied the production of the IGFBPs by human granulosa cells (GC) in culture and the role of IGFBP-3 in the modulatio...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03347856

    authors： Barreca A,Artini PG,Cesarone A,Arvigo M,D'Ambrogio G,Genazzani AR,Giordano G,Minuto F

    更新日期：1996-01-01 00:00:00

  • Primary hypothyroidism associated with secondary adrenocortical insufficiency.

    abstract：:A 50 year old man is described in whom primary hypothyroidism and isolated ACTH deficiency leading to adrenocortical insufficiency occurred simultaneously. Low thyroid hormone levels, elevated serum TSH values and high titers of antithyroid antibodies (ATA) were consistent with primary hypothyroidism. The diagnosis of...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03348331

    authors： Roosens B,Maes E,Van Steirteghem A,Vanhaelst L

    更新日期：1982-07-01 00:00:00

  • New insights into vitamin D regulation: is there a role for alkaline phosphatase?

    abstract：PURPOSE:The diagnosis of vitamin D deficiency is based on the determination of total plasma 25-hydroxyvitamin D (25-OHD) concentrations, but the regulation of vitamin D 25-hydroxylation is not a major consideration and very little information is available on this activity. To check what factors could interfere with the...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/s40618-021-01503-w

    authors： Bellastella G,Scappaticcio L,Longo M,Carotenuto R,Carbone C,Caruso P,Maio A,Paglionico VA,Vietri MT,Maiorino MI,Esposito K

    更新日期：2021-01-25 00:00:00

  • Growth hormone and lactate responses induced by maximal isometric voluntary contractions and whole-body vibrations in healthy subjects.

    abstract：BACKGROUND:In contrast with maximal voluntary resistance exercise, which is allegedly considered a potent GH stimulus in young subjects, evaluation of GH response to whole-body vibrations (WBV) has yielded conflicting results. METHODS:The acute effects of WBV alone (test A), maximal voluntary isometric contractions (M...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03347069

    authors： Sartorio A,Agosti F,De Col A,Marazzi N,Rastelli F,Chiavaroli S,Lafortuna CL,Cella SG,Rigamonti AE

    更新日期：2011-03-01 00:00:00

  • From inflammation to sexual dysfunctions: a journey through diabetes, obesity, and metabolic syndrome.

    abstract：:Metabolic diseases are associated with chronic low-grade inflammation, which has been indicated as a potential mediator of endothelial dysfunction and cardiovascular disease. Visceral adiposity is thought to be the starting condition of the inflammatory state through the release of inflammatory cytokines, including TN...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,评审

    doi：10.1007/s40618-018-0872-6

    authors： Maiorino MI,Bellastella G,Giugliano D,Esposito K

    更新日期：2018-11-01 00:00:00

  • Role of vitamin K on biochemical markers, bone mineral density, and fracture risk.

    abstract：:Osteoporosis is a multifactorial chronic disease that may become even more prevalent and more of a public health problem in the decades to come. Recent research has indicated that a number of macro- and micronutrients are involved in the development of bone health. In the past decade it became evident that vitamin K p...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,评审

    doi：

    authors： Francucci CM,Rilli S,Fiscaletti P,Boscaro M

    更新日期：2007-01-01 00:00:00

  • Supplementation with L-arginine favorably influences plasminogen activator inhibitor type 1 concentration in obese patients. A randomized, double blind trial.

    abstract：BACKGROUND:Elevated plasminogen activator inhibitor type 1 (PAI 1) plays an important role in the pathogenesis of excess blood coagulability in obese patients. L-arginine supplementation has shown to be associated with enhanced cardiovascular and metabolic health. The aim of the study was to assess the effect of L-argi...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,随机对照试验

    doi：10.3275/8467

    authors： Bogdanski P,Szulinska M,Suliburska J,Pupek-Musialik D,Jablecka A,Witmanowski H

    更新日期：2013-04-01 00:00:00

  • Pro-gamma-MSH levels in various disorders of pituitary-adrenal axis.

    abstract：:N-Terminal (1-76) portion of proopiomelanocortin (hNT) was measured in normals, Addison's, Nelson's, Cushing's disease, and in dexamethasone suppressible hyperaldosteronism (DSH) by using a specific homologous RIA. Mean basal immunoreactive hNT level was 94.2 +/- 6 pg/ml (mean +/- SE) in normal subjects. In Cushing's ...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03348575

    authors： Boscaro M,Giacomazzi P,Sonino N,Fallo F,Girard F,Bertagna X,Mantero F

    更新日期：1990-01-01 00:00:00

  • Impact of overweight and obesity on obstetric outcomes.

    abstract：:Abnormal nutritional status is an increasingly common complication in developed countries and, as reproductive age women are a part of this trend, the effect of maternal obesity on the pregnancy and neonate must be investigated. Pregestational obesity or overweight condition or an excessive gestational weight gain can...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,评审

    doi：10.1007/s40618-014-0058-9

    authors： Triunfo S,Lanzone A

    更新日期：2014-04-01 00:00:00

  • The inhibitory effect of glucose on growth hormone secretion is lost in obesity but not in hypertension.

    abstract：:In obesity there is a clear reduction of both spontaneous and stimulated GH secretion. Furthermore, in obese patients the somatotrope responsiveness to provocative stimulation is selectively refractory to the inhibitory effect of glucose load. It has been hypothesized that hyperinsulinism of obese patients could play ...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03346919

    authors： Limone P,Oleandri SE,Ajmone Catt P,Grottoli S,Frangioni C,Avogadri E,Perrin M,Valetto MR,Maccario M

    更新日期：1997-11-01 00:00:00

  • Type 2 diabetes mellitus in children and adolescents.

    abstract：:Over the last decade, there has been an alarming increase of Type 2 diabetes mellitus (T2DM) in youths, concomitant with the rise of obesity in this age group. T2DM is a progressive disease with a gradual increase in insulin resistance associated later with a decline in insulin secretion with fasting hyperglycemia. Pr...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章,评审

    doi：10.1007/BF03347581

    authors： Marcovecchio M,Mohn A,Chiarelli F

    更新日期：2005-10-01 00:00:00

  • Thyrotropin receptor-adenylate cyclase system in plasma membranes from normal and diseased human thyroid glands.

    abstract：:Thyrotropin binding characteristics and adenylate cyclase (AC) activity of thyroid plasma membranes were studied in 52 tissues from normal and diseased human thyroids. Data from normal glands, Graves' goiters, non toxic multinodular goiters and nodular and perinodular tissue of toxic nodular goiters show the same basa...

    journal_title：Journal of endocrinological investigation

    pub_type： 杂志文章

    doi：10.1007/BF03350977

    authors： Carayon P,Guibout M,Lissitzky S

    更新日期：1978-10-01 00:00:00