Abstract:
:As it has been hypothesized that IGF-binding proteins (IGFBPs) may have a role as autocrine/paracrine factors in regulating the local actions of the insulin-like growth factors (IGFs) in the ovary, we studied the production of the IGFBPs by human granulosa cells (GC) in culture and the role of IGFBP-3 in the modulation of ovarian cell responsiveness to IGF-I and FSH. To this purpose, human luteinizing GC were cultured in serum-free conditions for 24 h and subsequently submitted to increasing concentrations (2-8 nmol/l) of recombinant non-glycosylated or partially glycosylated IGF-BP-3 for 48 h, in the presence or absence of IGF-I, des(1-3)IGF-I- a truncated analog of human IGF-I with markedly reduced binding ability to IGFBPs - and FSH (5-20 mIU/ml). The results demonstrate that human GC release IGFBP-1-2 and -3 into the medium, and that FSH is able to inhibit this release, while GH is clearly inhibitory on IGFBP-1 and stimulatory on IGFBP-3. Both IGF-I and des(1-3)IGF-I significantly (p < 0.001) stimulate E2 production by human GC in culture in a manner comparable to that of FSH in the dose range used. Preincubation for 2 h at 22 C with IGFBP-3, to allow the formation of the IGF-IGFBP complex, drastically reduced the stimulatory effect of IGF-I but not that of des(1-3)IGF-I. IGFBP-3 was also able to inhibit the stimulatory effect of FSH. These data show that: i) the IGF peptide is less active when bound to IGFBP-3; ii) as IGFBP-3 does not affect the potency of des(1-3)IGF-I, its inhibitory action is exerted upstream of the membrane receptor binding; iii) as the action of IGFBP-3 is exerted by binding the IGF peptide, its inhibitory effect on FSH points out the role of the locally produced IGF-II in potentiating the FSH action on human GC.
journal_name
J Endocrinol Investjournal_title
Journal of endocrinological investigationauthors
Barreca A,Artini PG,Cesarone A,Arvigo M,D'Ambrogio G,Genazzani AR,Giordano G,Minuto Fdoi
10.1007/BF03347856subject
Has Abstractpub_date
1996-01-01 00:00:00pages
35-42issue
1eissn
0391-4097issn
1720-8386journal_volume
19pub_type
杂志文章abstract::Central hypothyroidism (CH) is a rare cause of hypothyroidism characterized by a defect of thyroid hormone production due to an insufficient TSH stimulation. CH can be congenital in the case of genetic defects or acquired in the case of lesions affecting either pituitary or hypothalamus. Diagnosis is usually made on a...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:
更新日期:2008-09-01 00:00:00
abstract:BACKGROUND AND AIM:There is limited data on the effects of inactivity (prolonged bed-rest) on parameters of endocrine and metabolic function; we therefore aimed to examine changes in these systems during and after prolonged (56- day) bed-rest in male adults. SUBJECTS AND METHODS:Twenty healthy male subjects underwent ...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,随机对照试验
doi:10.3275/7606
更新日期:2012-01-01 00:00:00
abstract::Gonadotropins control male and female gonadal function by acting through specific receptors. The recent description of several mutations in LH and FSH receptors has significantly improved our understanding of the pathophysiology of several sexual disorder. Both gain- and loss-of-function germline mutations leading to ...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/BF03343777
更新日期:2000-10-01 00:00:00
abstract:PURPOSE:To determine the incidence and determinants of developing abnormalities on the 12-lead electrocardiogram (ECG) in persons with type 1 diabetes. METHODS:We evaluated the distribution of ECG abnormalities and risk factors for developing new abnormalities in 266 (mean age = 44 years ± 9.0; 50 % female) people wit...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-016-0564-z
更新日期:2017-03-01 00:00:00
abstract:PURPOSE:Achieving biochemical control (normalization of insulin-like growth factor-1 [IGF-1] and growth hormone [GH]) is a key goal in acromegaly management. However, IGF-1 and GH fluctuate over time. The true potential impact of time-varying biochemical control status on comorbidities is unclear and relies on multiple...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-019-01138-y
更新日期:2020-04-01 00:00:00
abstract::We report here on the isolation and repertoire characterization of a Graves' thyroid T cell population which, unlike most Graves' thyroid T cell isolates reported before, was rich in CD4-CD8- T cells (16%, all TcR gamma delta+) and CD8+ T cells (75%) [TcR alpha beta+ (59%) and TcR gamma delta+ (16%)]. Only 7% of the i...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03348958
更新日期:1993-12-01 00:00:00
abstract::The aim of this study was to investigate the effects of age, duration of diabetes, sex and ICA (Islet cell cytoplasmic antibody) on beta-cell reserves and to develop a model within the first 2 years of Type 1 diabetes. Beta-cell reserve is evaluated as fasting (FCp) and 1 mg i.v. glucagon stimulated C-peptide (SCp) le...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03344073
更新日期:2002-12-01 00:00:00
abstract:PURPOSE:Uncertainties exist about the predictors of the severity of the clinical picture of GH deficiency (GHD) syndrome. Aim of the study was to evaluate, in adult patients with GHD, the predictors of the development of hypercholesterolemia, hypertension, diabetes mellitus, and osteoporosis. METHODS:We retrospectivel...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-019-01100-y
更新日期:2020-02-01 00:00:00
abstract::Severe vitamin D deficiency has been found to be prevalent in institutionalized elderly persons in several countries. The aim of the present work was to assess the vitamin D status of institutionalized elderly and compare it to that of community-living independent elderly in southern Greece during summer. Serum 25-hyd...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03349258
更新日期:2008-09-01 00:00:00
abstract:BACKGROUND:Successful parathyroidectomy (PTX) often results in a dramatic drop in the parathyroid hormone (PTH) levels, relieves the patient from clinical symptoms, and reduces mortality. Although PTX is generally a successful treatment for progressive secondary hyperparathyroidism (SHPT) patients subjected to surgery,...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/s40618-015-0410-8
更新日期:2016-05-01 00:00:00
abstract::Plasma levels and 24-h urinary excretion of cyclic AMP and cyclic GMP were measured in 18 patients with hyperthyroidism, 7 patients with hypothyroidism and 25 normal subjects. Mean plasma and urinary levels of both cyclic AMP and cyclic GMP were significantly positive correlations between the serum thyroid hormone lev...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03350603
更新日期:1983-06-01 00:00:00
abstract::Dopamine in humans inhibits the secretion of luteinizing hormone (LH), follicular stimulating hormone (FSH), thyroid stimulating hormone (TSH) and prolactin (PRL), and is a stimulator of growth hormone (GH) secretion. Dopamine-D1 receptor stimulation with fenoldopam increases basal PRL levels, suppresses TSH, and incr...
journal_title:Journal of endocrinological investigation
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1007/BF03350331
更新日期:1998-05-01 00:00:00
abstract:BACKGROUND:Visfatin [pre-B cell colony- enhancing factor (PBEF)] is a cytokine highly expressed in visceral fat that exhibits insulin-mimetic properties. However, its role in insulin-resistant states, such as in metabolic syndrome (MetS), remains largely unknown. OBJECTIVE:To investigate the possible differences of pl...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03346300
更新日期:2007-04-01 00:00:00
abstract::E-cadherin and catenins play a major role in neoplastic cell behavior as a suppressor of invasion and/or metastasis. The aim of this study was to determine E-cadherin, alpha-catenin and beta-catenin expressions in papillary thyroid carcinoma (PTC) and to correlate the results of expression to initial clinicopathologic...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03345079
更新日期:2002-07-01 00:00:00
abstract::Estrogen deficiency in females and androgen deficiency in males may harm periosteal and endosteal apposition, reduce bone size and both cortical and trabecular thickness, modifying in this way the bone structure later in life. To date, few systematic studies on bone mineral density (BMD) and hypogonadism in adolescent...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03346513
更新日期:2009-07-01 00:00:00
abstract:BACKGROUND:Agents capable of increasing radioiodine concentration by stimulating the sodium/iodide symporter (NIS) expression have been extensively investigated for the treatment of certain well-differentiated breast cancers. AIM:In this study, we analyzed the regulation of the NIS and lactoperoxidase (LPO) gene expre...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03346542
更新日期:2010-01-01 00:00:00
abstract::An immunohistochemical study with two rabbit polyclonal antibodies I-AR76 and CA-08-351 against Endothelin-1 (ET-1) was performed in 133 human thyroid specimens: 5 normal thyroids, 30 multinodular goiters (15 toxic and 15 nontoxic), 20 Graves' diseases, 5 Hashimoto's thyroiditis, 26 adenomas (6 Hürthle cell, 16 toxic ...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03347834
更新日期:1995-05-01 00:00:00
abstract:CONTEXT:The antiproliferative mechanism of mycophenolate acid (MPA) suggests a beneficial effect in patients with Graves' orbitopathy (GO). OBJECTIVE:To systematically analyze for the first time adverse events (AEs) during MPA treatment in GO. DESIGN:Prospective longitudinal study. SETTING:Academic tertiary referral...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-016-0441-9
更新日期:2016-06-01 00:00:00
abstract::We report an original application of quantitative reverse transcription-polymerase chain reaction (Q.RT-PCR) for the evaluation of atrial natriuretic factor (ANF), brain natriuretic peptide (BNP) and beta-actin mRNA in small atrial samples (10-15 mg). A fixed amount of total RNA was simultaneously reverse transcribed ...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03343655
更新日期:1999-12-01 00:00:00
abstract::The 24-h profiles of plasma cortisol (F), 11-beta-hydroxyandrostenedione (11OHAD), androstenedione (AD), dehydroisoandrosterone (DHEA) and testosterone (T) were obtained simultaneously in 11 normal males sampled at 15-min intervals. The data were submitted to a detailed quantitative analysis including the estimation o...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03348129
更新日期:1987-06-01 00:00:00
abstract::A 50 year old man is described in whom primary hypothyroidism and isolated ACTH deficiency leading to adrenocortical insufficiency occurred simultaneously. Low thyroid hormone levels, elevated serum TSH values and high titers of antithyroid antibodies (ATA) were consistent with primary hypothyroidism. The diagnosis of...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03348331
更新日期:1982-07-01 00:00:00
abstract::Thyroid hormone acts on the oocytes, sperm and embryo during fertilization, implantation and placentation. Both hypothyroidism and hyperthyroidism may influence fertility. However, evidence of the association of hyperthyroidism with infertility is scarce and sometimes conflicting. Thyroid hormone influences human repr...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,评审
doi:10.1007/s40618-016-0452-6
更新日期:2016-08-01 00:00:00
abstract::To evaluate the influence of chronological age and pubertal development on the hypothalamus-pituitary-adrenal (HPA) axis response to stress, we studied the possible correlations between male pubertal characteristics and salivary cortisol (C), DHEAS and the DHEAS/C ratio before (pre-stress) and after acute exercise-str...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03347373
更新日期:2006-10-01 00:00:00
abstract:BACKGROUND:Adiponectin has emerged over the last decade as a key adipokine linking obesity, insulin resistance, and Type 2 diabetes. However, the molecular mechanisms controlling adiponectin expression in adipose tissue are not fully elucidated. Furthermore, increasing evidence indicates that peroxisome proliferator-ac...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03347056
更新日期:2011-02-01 00:00:00
abstract::Sleep-related breathing disorders are recognized as major health problems in obesity. They are involved in both hypertension and Type 2 diabetes, through mechanisms possibly related to increased sympathetic tone. We studied the association of habitual snoring with diabetes, hypertension, weight cycling and physical ac...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03346260
更新日期:2004-02-01 00:00:00
abstract:PURPOSE:Although vitamin D is reportedly associated with various cancers, the association between vitamin D and thyroid cancer is indefinite. We aimed to investigate whether this association applies to thyroid cancer (TC). METHODS:A total of 276 Chinese Han people were recruited in a current matched case-control study...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,meta分析
doi:10.1007/s40618-018-0853-9
更新日期:2018-10-01 00:00:00
abstract:PURPOSE:Leptin is associated with cardiovascular risk factors (e.g. hypertension, insulin resistance, kidney disease and excess body weight). Experimental studies showed that leptin might affect serum uric acid, by modulation of the uric acid excretion. However, there are few observational data on the relationship betw...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-019-01140-4
更新日期:2020-05-01 00:00:00
abstract:PURPOSE:Iodine plays a pivotal role in adaptation during the transition from intrauterine to extrauterine life. Although it is well known that the placenta plays a role in iodine storage, a relationship between the neonatal thyroid stimulating hormone (TSH) peak and placental iodine concentration has not been establish...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/s40618-020-01399-y
更新日期:2020-08-28 00:00:00
abstract:BACKGROUND:Hyperglycemia is a common, but not well-characterized side effect of glucocorticoid treatment. AIM:To study the effect of pulse dexamethasone treatment on carbohydrate metabolism among multiple myeloma patients. MATERIAL/SUBJECTS AND METHODS:A randomized crossover observational study in a teaching hospital...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章,随机对照试验
doi:10.1007/s40618-013-0027-8
更新日期:2014-04-01 00:00:00
abstract::The activities of total superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) enzymes were measured in cancerous and non-cancerous adjacent tissues from 15 patients with follicular thyroid cancer containing single nodule. SOD and GSH-Px activities were found lower but malondialdehyde levels hi...
journal_title:Journal of endocrinological investigation
pub_type: 杂志文章
doi:10.1007/BF03347868
更新日期:1996-05-01 00:00:00