Abstract:
Background:Bridging integrator 1 (BIN1) has showed outstanding tumor-suppressive potential via inhibiting c-MYC-mediated tumorigenesis. However, a frequent phosphorylation of c-MYC at Ser-62 site could block the BIN1/c-MYC interaction and limits the tumor-suppressive effect of BIN1. Cyclin-dependent kinase 5 (CDK5), a generally dysregulated protein in various carcinomas, can mediate c-MYC phosphorylation at Ser-62 site. However, whether the existence of CDK5 could block the BIN1/c-MYC interaction remains unclear. Materials and methods:The expression of CDK5 and BIN1 in non-small cell lung cancer (NSCLC) cell lines were measured. CDK5 was knocked down and overexpressed in H460 and PC9 cells, respectively. CCK-8, wound healing and transwell were used to detect the proliferation, migration and invasion ability of NSCLC cells. Tumor-bearing nude mouse model was built with H460 cells. Dinaciclib was added to realize the effect of CDK5 inhibition in vivo. NSCLC and matched para-carcinoma specimens were collected from 153 patients who underwent radical operation. IHC was performed to determine the expression of CDK5 in the specimens. Kaplan-Meier analysis was used to analyze the correlation between the postoperative survival and CDK5 expression. Results:CDK5 was highly expressed in H460 cells, and knockdown of CDK5 could restore the BIN1/c-MYC interaction. Meanwhile, low expression of CDK5 was observed in PC9 cells, and overexpression of CDK5 blocked the BIN1/c-MYC interaction. Consequently, the growth, migration, invasion and epithelial mesenchymal transition (EMT) ability of H460 and PC9 cells could be facilitated by CDK5. The addition of CDK5 inhibitor Dinaciclib significantly suppressed the tumorigenesis ability of NSCLC cells in tumor-bearing mouse model. Furthermore, high expression of CDK5, along with low expression of BIN1, could predict poor postoperative prognosis of NSCLC patients. The patients with high expression of CDK5 and low expression of BIN1 showed similar prognosis, indicating that CDK5 could neutralize the tumor suppressing effect of BIN1 in clinical situation. Conclusions:CDK5 blocked the interaction of BIN1 and c-MYC via promoting phosphorylation of c-MYC at ser-62 site, ultimately facilitated the progression of NSCLC.
journal_name
Cancer Cell Intjournal_title
Cancer cell internationalauthors
Zhang X,Wang J,Jia Y,Liu T,Wang M,Lv W,Zhang R,Shi J,Liu Ldoi
10.1186/s12935-019-0952-5subject
Has Abstractpub_date
2019-09-02 00:00:00pages
226issn
1475-2867pii
952journal_volume
19pub_type
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journal_title:Cancer cell international
pub_type: 杂志文章
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journal_title:Cancer cell international
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journal_title:Cancer cell international
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journal_title:Cancer cell international
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journal_title:Cancer cell international
pub_type: 杂志文章
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journal_title:Cancer cell international
pub_type: 杂志文章
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journal_title:Cancer cell international
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更新日期:2015-09-17 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-13-30
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-016-0331-4
更新日期:2016-08-25 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-12-2
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journal_title:Cancer cell international
pub_type: 杂志文章
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journal_title:Cancer cell international
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-018-0628-6
更新日期:2018-09-03 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/1475-2867-2-1
更新日期:2002-03-07 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-017-0453-3
更新日期:2017-09-26 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-020-1119-0
更新日期:2020-02-19 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-017-0452-4
更新日期:2017-10-24 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章,评审
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更新日期:2020-11-04 00:00:00
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journal_title:Cancer cell international
pub_type: 杂志文章
doi:10.1186/s12935-017-0457-z
更新日期:2017-10-04 00:00:00