Real-time RT-PCR analysis of mRNA decay: half-life of Beta-actin mRNA in human leukemia CCRF-CEM and Nalm-6 cell lines.


:BACKGROUND: We describe an alternative method to determine mRNA half-life (t1/2) based on the Real-Time RT-PCR procedure. This approach was evaluated by using the beta-actin gene as a reference molecule for measuring of mRNA stability. RESULTS: Human leukemia Nalm-6 and CCRF-CEM cells were treated with various concentrations of Actinomycin D to block transcription and aliquots were removed periodically. Total RNA was isolated and quantified using the RiboGree&ncircledR; fluorescent dye with the VersaFluor Fluorometer System. One &mgr;g of total RNA was reverse transcribed and used as template for the amplification of a region of the beta-actin gene (231 bp). To generate the standard curve, serial ten-fold dilutions of the pBactin-231 vector containing the cDNA amplified fragment were employed, beta-actin mRNAs were quantified by Real-Time RT-PCR using the SYB&RcircledR; Green I fluorogenic dye and data analyzed using the iCycle iQ system software. Using this method, the beta-actin mRNA exhibited a half-life of 6.6 h and 13.5 h in Nalm-6 and CCRF-CEM cells, respectively. The t1/2 value obtained for Nalm-6 is comparable to those estimated from Northern blot studies, using normal human leukocytes (5.5 h). CONCLUSIONS: We have developed a rapid, sensitive, and reliable method based on Real-Time RT-PCR for measuring mRNA half-life. Our results confirm that beta-actin mRNA half-life can be affected by the cellular growth rate.


Cancer Cell Int


Leclerc GJ,Leclerc GM,Barredo JC





Has Abstract


2002-03-07 00:00:00










  • microRNA-365 inhibits YAP through TLR4-mediated IRF3 phosphorylation and thereby alleviates gastric precancerous lesions.

    abstract:BACKGROUND:Gastric carcinoma (GC) is currently one of the most common malignant tumors of the digestive system, and gastric precancerous lesions play a vital role in studying the mechanism of GC. Multiple microRNAs (miRNAs) have been documented to be potential biomarkers to indicate progression of gastric precancerous ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zhang T,Zhang K,Ji K,Zhang C,Jiang Y,Zhang Q,Tian Z,Wang X,Zhang M,Li X

    更新日期:2020-11-13 00:00:00

  • TMPO-AS1 promotes cell proliferation of thyroid cancer via sponging miR-498 to modulate TMPO.

    abstract:Background:Thyroid cancer (TC) is the most frequent endocrine malignancy. Long noncoding RNAs (lncRNAs) have been confirmed to act as significant roles in tumor development. The role of lncRNA TMPO-AS1 in TC is still unclear, so it remains to be explored. The aim of the research is to investigate the role and regulator...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Li Z,Feng Y,Zhang Z,Cao X,Lu X

    更新日期:2020-07-08 00:00:00

  • The mRNA expression of SATB1 and SATB2 in human breast cancer.

    abstract:BACKGROUND:SATB1 is a nuclear protein that has been recently reported to be a 'genome organizer' which delineates specific epigenetic modifications at target gene loci, directly up-regulating metastasis-associated genes while down-regulating tumor-suppressor genes. In this study, the level of mRNA expression of SATB1 a...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Patani N,Jiang W,Mansel R,Newbold R,Mokbel K

    更新日期:2009-07-30 00:00:00

  • Epigallocatechin-3-gallate inhibits the growth and increases the apoptosis of human thyroid carcinoma cells through suppression of EGFR/RAS/RAF/MEK/ERK signaling pathway.

    abstract:Background:Thyroid cancer is the most common type of endocrine malignancy and the incidence rate is rapidly increasing worldwide. Epigallocatechin-3-gallate (EGCG) could suppress cancer growth and induce apoptosis in many types of cancer cells. However, the mechanism of action of EGCG on the growth of human thyroid car...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Wu D,Liu Z,Li J,Zhang Q,Zhong P,Teng T,Chen M,Xie Z,Ji A,Li Y

    更新日期:2019-02-28 00:00:00

  • CircNEIL3 promotes cervical cancer cell proliferation by adsorbing miR-137 and upregulating KLF12.

    abstract:BACKGROUND:CircRNAs play crucial roles in multiple tumours. However, the functions of most circRNAs in cervical cancer remain unclear. METHODS:This study collected GSE113696 data from the GEO database to search for differentially expressed circRNAs in cervical cancer. Quantitative reverse transcription PCR was used to...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Chen Y,Geng Y,Huang J,Xi D,Xu G,Gu W,Shao Y

    更新日期:2021-01-07 00:00:00

  • Apigenin promotes apoptosis, inhibits invasion and induces cell cycle arrest of T24 human bladder cancer cells.

    abstract:BACKGROUND:Apigenin (4',5,7-trihydroxyflavone) was recently shown effective in inhibiting several cancers. The aim of this study was to investigate the effect and mechanism of apigenin in the human bladder cancer cell line T24 for the first time. METHODS:T24 cells were treated with varying concentrations and time of a...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zhu Y,Mao Y,Chen H,Lin Y,Hu Z,Wu J,Xu X,Xu X,Qin J,Xie L

    更新日期:2013-06-01 00:00:00

  • Investigation of the apoptotic way induced by digallic acid in human lymphoblastoid TK6 cells.

    abstract:BACKGROUND:The digallic acid (DGA) purified from Pistacia lentiscus. L fruits was investigated for its antiproliferative and apoptotic activities on human lymphoblastoid TK6 cells. METHODS:We attempt to characterize the apoptotic pathway activated by DGA. Apoptosis was detected by DNA fragmentation, PARP cleavage and ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Bhouri W,Boubaker J,Skandrani I,Ghedira K,Chekir Ghedira L

    更新日期:2012-06-11 00:00:00

  • miR-133a function in the pathogenesis of dedifferentiated liposarcoma.

    abstract:Background:Sarcomas are malignant heterogeneous tumors of mesenchymal derivation. Dedifferentiated liposarcoma (DDLPS) is aggressive with recurrence in 80% and metastasis in 20% of patients. We previously found that miR-133a was significantly underexpressed in liposarcoma tissues. As this miRNA has recently been shown ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Yu PY,Lopez G,Braggio D,Koller D,Bill KLJ,Prudner BC,Zewdu A,Chen JL,Iwenofu OH,Lev D,Strohecker AM,Fenger JM,Pollock RE,Guttridge DC

    更新日期:2018-06-26 00:00:00

  • Phorbol myristate acetate and Bryostatin 1 rescue IFN-gamma inducibility of MHC class II molecules in LS1034 colorectal carcinoma cell line.

    abstract:BACKGROUND:The expression of major histocompatibility complex class II (MHCII) antigens in both mouse and human tumors is rare, and these antigens are not easily inducible by IFN-gamma (IFNg). Since MHCII may play an important role in the development of host antitumor immune response, we explored the possibility of res...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Kudinov Y,Wiseman CL,Kharazi AI

    更新日期:2003-03-25 00:00:00

  • Repression of GPRC5A is associated with activated STAT3, which contributes to tumor progression of head and neck squamous cell carcinoma.

    abstract:BACKGROUND:G protein-coupled receptor family C group 5 member A (GPRC5A), a retinoic acid-inducible gene, is a lung tumor suppressor. Previously, we showed that repression of GPRC5A expression was associated with pathologic differentiation grade of oral squamous cell carcinomas (OSCC) and overexpression of GPRC5A gene ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Liu S,Ye D,Wang T,Guo W,Song H,Liao Y,Xu D,Zhu H,Zhang Z,Deng J

    更新日期:2017-03-02 00:00:00

  • The heterogeneous clinical and pathological landscapes of metastatic Braf-mutated colorectal cancer.

    abstract::Colorectal cancer (CRC) is a complex and molecularly heterogeneous disease representing one of the most frequent causes of cancer-related death worldwide. About 8-15% of CRCs harbor a mutation in BRAF gene, a proto-oncogene involved in cell proliferation, differentiation and survival through the MAPK signaling cascade...

    journal_title:Cancer cell international

    pub_type: 杂志文章,评审


    authors: Fanelli GN,Dal Pozzo CA,Depetris I,Schirripa M,Brignola S,Biason P,Balistreri M,Dal Santo L,Lonardi S,Munari G,Loupakis F,Fassan M

    更新日期:2020-01-29 00:00:00

  • Differential effects of HIF-α isoforms on apoptosis in renal carcinoma cell lines.

    abstract:BACKGROUND:Germline mutations in the von Hippel-Lindau (VHL) tumor suppressor gene predispose individuals to clear cell renal carcinomas, hemangioblastomas, and pheochromocytomas. The VHL gene product forms an ubiquitin E3 ligase complex, with regulation of hypoxia-inducible factor alpha (HIF-α) as its best known funct...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Doonachar A,Gallo MD,Doukas D,Pasricha R,Lantsberg I,Schoenfeld AR

    更新日期:2015-02-22 00:00:00

  • Antibiotics inhibit sphere-forming ability in suspension culture.

    abstract:BACKGROUND:This last decade, a lot of emphasis has been placed on developing new cancer cell culture models, closer to in vivo condition, in order to test new drugs and therapies. In the case of colorectal cancer, the use of patient biopsies to seed 3D primary cultures and mimic tumor initiation necessitates the use of...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Relier S,Yazdani L,Ayad O,Choquet A,Bourgaux JF,Prudhomme M,Pannequin J,Macari F,David A

    更新日期:2016-02-12 00:00:00

  • YY1-induced lncRNA ZFPM2-AS1 facilitates cell proliferation and invasion in small cell lung cancer via upregulating of TRAF4.

    abstract:Background:Newly identified lncRNA zinc finger protein, FOG family member 2 antisense RNA 1 (ZFPM2-AS1) is identified as an oncogenic gene. However, the role of ZFPM2-AS1 in small cell lung cancer (SCLC) is poorly comprehended. Methods:The expression of genes in SCLC tissues and cells was measured by qRT-PCR. Colony f...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Yan Z,Yang Q,Xue M,Wang S,Hong W,Gao X

    更新日期:2020-04-03 00:00:00

  • Increased expression of Six1 correlates with progression and prognosis of prostate cancer.

    abstract::Sineoculis homeobox homolog 1 (Six1), normally a developmentally restricted transcriptional regulator, is frequently dysregulated in mutiple cancers. Increasing evidences show that overexpression of Six1 plays a key role in tumorigenesis. However, the Six1 expression status and its relationship with the clinicopatholo...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zeng J,Shi R,Cai CX,Liu XR,Song YB,Wei M,Ma WL

    更新日期:2015-06-20 00:00:00

  • Potential Role of circPVT1 as a proliferative factor and treatment target in esophageal carcinoma.

    abstract:Background:Many circRNAs have been reported to play important roles in cancer development and have the potential to serve as a novel class of biomarkers for clinical diagnosis. However, the role of circRNAs in esophageal carcinoma (EC) remains unclear. In the current study, we investigated the potential role of circPVT...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zhong R,Chen Z,Mo T,Li Z,Zhang P

    更新日期:2019-10-15 00:00:00

  • Reshaping the role of m6A modification in cancer transcriptome: a review.

    abstract::N6-methyl-adenosine(m6A) modification emerges as an abundant and dynamic regulation throughout the Eukaryotic transcriptome. Dysregulation of the m6A regulators has increasingly been found in many neoplasms. It is reasonable to believe that m6A changes the fate of cancer cells and subsequently affected all aspects of ...

    journal_title:Cancer cell international

    pub_type: 杂志文章,评审


    authors: Yang G,Sun Z,Zhang N

    更新日期:2020-07-29 00:00:00

  • Melatonin and non-small cell lung cancer: new insights into signaling pathways.

    abstract::Non-small-cell lung cancer (NSCLC) is a type of malignancy with progressive metastasis having poor prognosis and lowered survival resulting from late diagnosis. The therapeutic approaches for the treatment of this incurable cancer are chemo- and radiotherapy. Since current treatments are insufficient and because of dr...

    journal_title:Cancer cell international

    pub_type: 杂志文章,评审


    authors: Pourhanifeh MH,Sharifi M,Reiter RJ,Davoodabadi A,Asemi Z

    更新日期:2019-05-16 00:00:00

  • TP53 mutant cell lines selected for resistance to MDM2 inhibitors retain growth inhibition by MAPK pathway inhibitors but a reduced apoptotic response.

    abstract:Background:Emergence of resistance to molecular targeted therapy constitutes a limitation to clinical benefits in cancer treatment. Cross-resistance commonly happens with chemotherapeutic agents but might not with targeted agents. Methods:In the current study, TP53 wild-type cell lines with druggable MAPK pathway muta...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Wu CE,Koay TS,Ho YH,Lovat P,Lunec J

    更新日期:2019-03-07 00:00:00

  • Caspase-2 is involved in cell death induction by taxanes in breast cancer cells.

    abstract:BACKGROUND:We studied the role of caspase-2 in apoptosis induction by taxanes (paclitaxel, novel taxane SB-T-1216) in breast cancer cells using SK-BR-3 (nonfunctional p53, functional caspase-3) and MCF-7 (functional p53, nonfunctional caspase-3) cell lines. RESULTS:Both taxanes induced apoptosis in SK-BR-3 as well as ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Jelínek M,Balušíková K,Kopperová D,Nĕmcová-Fürstová V,Šrámek J,Fidlerová J,Zanardi I,Ojima I,Kovář J

    更新日期:2013-05-15 00:00:00

  • MicroRNA-222 influences migration and invasion through MIA3 in colorectal cancer.

    abstract:BACKGROUND:miR-222 has been reported to be overexpressed in colorectal cancer and it influences cancer cell proliferation, drug resistance and metastasis. However, the underlying molecular mechanism of miR-222 in colorectal cancer cell invasion and migration has not been thoroughly elucidated to date. METHODS:The cell...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Gao H,Cong X,Zhou J,Guan M

    更新日期:2017-08-29 00:00:00

  • Transgelin interacts with PARP1 in human colon cancer cells.

    abstract:Background:Transgelin, an actin-binding protein, is associated with cytoskeleton remodeling. Findings from our previous studies demonstrated that transgelin was up-regulated in node-positive colorectal cancer (CRC) versus node-negative disease. Over-expression of TAGLN affected the expression of 256 downstream transcri...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Lew ZX,Zhou HM,Fang YY,Ye Z,Zhong W,Yang XY,Yu Z,Chen DY,Luo SM,Chen LF,Lin Y

    更新日期:2020-08-03 00:00:00

  • Comprehensive analysis of prognostic alternative splicing signature in cervical cancer.

    abstract:Background:Alternative splicing (AS) is a key factor in protein-coding gene diversity, and is associated with the development and progression of malignant tumours. However, the role of AS in cervical cancer is unclear. Methods:The AS data for cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC) were...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Ouyang D,Yang P,Cai J,Sun S,Wang Z

    更新日期:2020-06-08 00:00:00

  • Relationship between TRAF6 and deterioration of HCC: an immunohistochemical and in vitro study.

    abstract:OBJECTIVE:To explore the relationship between tumor necrosis factor receptor-associated factor 6 (TRAF6) and the clinicopathological features in HCC as well as its biological function. METHODS:Totally, 412 liver tissues were collected, including 171 hepatocellular carcinoma (HCC) and their corresponding non-tumor tiss...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Li JJ,Luo J,Lu JN,Liang XN,Luo YH,Liu YR,Yang J,Ding H,Qin GH,Yang LH,Dang YW,Yang H,Chen G

    更新日期:2016-09-29 00:00:00

  • Survivin: a unique target for tumor therapy.

    abstract::Survivin is the smallest member of the Inhibitor of apoptosis (IAP) family of proteins, involved in inhibition of apoptosis and regulation of cell cycle. These functional attributes make Survivin a unique protein exhibiting divergent functions i.e. regulating cell proliferation and cell death. Expression pattern of Su...

    journal_title:Cancer cell international

    pub_type: 杂志文章,评审


    authors: Garg H,Suri P,Gupta JC,Talwar GP,Dubey S

    更新日期:2016-06-23 00:00:00

  • A signature of hypoxia-related factors reveals functional dysregulation and robustly predicts clinical outcomes in stage I/II colorectal cancer patients.

    abstract:Background:The hypoxic tumor microenvironment accelerates the invasion and migration of colorectal cancer (CRC) cells. The aim of this study was to develop and validate a hypoxia gene signature for predicting the outcome in stage I/II CRC patients that have limited therapeutic options. Methods:The hypoxic gene signatu...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Zou YF,Rong YM,Tan YX,Xiao J,Yu ZL,Chen YF,Ke J,Li CH,Chen X,Wu XJ,Lan P,Lin XT,Gao F

    更新日期:2019-09-23 00:00:00

  • Polymorphisms of TGFBR1, TLR4 are associated with prognosis of gastric cancer in a Chinese population.

    abstract:Background:Helicobacter pylori (H. pylori)-induced gastric cancer is an intricate progression of immune response against H. pylori infection. IL-16, TGF-β1 and TLR4 pathways were the mediators involved in the immune response. We hypothesized that genetic variations in genes of these pathways have potential susceptibili...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: He B,Xu T,Pan B,Pan Y,Wang X,Dong J,Sun H,Xu X,Liu X,Wang S

    更新日期:2018-11-20 00:00:00

  • Casticin inhibits nasopharyngeal carcinoma growth by targeting phosphoinositide 3-kinase.

    abstract:Background:Casticin, an isoflavone compound extracted from the herb Fructus Viticis, has demonstrated anti-inflammatory and anticancer activities and properties. The aim of this study was to investigate the effects and mechanisms of casticin in nasopharyngeal carcinoma (NPC) cells and to determine its potential for tar...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Liu J,Yang J,Hou Y,Zhu Z,He J,Zhao H,Ye X,Li D,Wu Z,Huang Z,Hao B,Yao K

    更新日期:2019-12-21 00:00:00

  • Observations on the expression of human papillomavirus major capsid protein in HeLa cells.

    abstract:BACKGROUND:The goal of this study was to identify the nature of the inclusion bodies that have been found in HeLa cells (cervical cancer immortal cell line) by electron microscope and to determine whether the major capsid protein (L1) of human papillomavirus (HPV) can be expressed in HPV-positive uterine cervix cancer ...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Xiao CY,Fu BB,Li ZY,Mushtaq G,Kamal MA,Li JH,Tang GC,Xiao SS

    更新日期:2015-05-23 00:00:00

  • MiR-544 promotes immune escape through downregulation of NCR1/NKp46 via targeting RUNX3 in liver cancer.

    abstract:Objective:To study the potential role of miR-544 in the immune escape mechanism of hepatoma cells. Methods:Natural killer (NK) cells were collected from healthy volunteers and patients with liver cancer. Interleukin (IL)-2 activated-NK-92 cells were transfected with miR-544 inhibitor/mimic or NC/pre-NC in HepG2 co-cul...

    journal_title:Cancer cell international

    pub_type: 杂志文章


    authors: Pan C,Xiang L,Pan Z,Wang X,Li J,Zhuge L,Fang P,Xie Q,Hu X

    更新日期:2018-04-03 00:00:00