Abstract：
BACKGROUND:Curcumin, a natural compound derived from the turmeric rhizome Curcuma longa Linn, has anticancer and chemoresistance reduction biological activities. We evaluated the efficacy of curcumin in sensitizing chemotherapy drugs through regulation of Bcl-2-mediated apoptosis in breast cancer stem-like cells (BCSCs). METHODS:Cell survival was measured using MTT assay. Apoptosis-related proteins were observed using western blot analysis. Apoptosis was detected with flow cytometric analysis and by Hoechst 33258 staining. The mitochondrial membrane potential was observed with flow cytometric analysis. RESULTS:The ability of BCSCs to propagate decreased gradually along the passages and was completely lost at the fifth passage [0.1 μmol/L mitomycin C (MMC) with 5 μmol/L curcumin in MCF-7 and 0.5 μmol/L MMC with 5 μmol/L curcumin in MDA-MB-231 cells]. Curcumin combined with MMC treatment significantly decreased the levels of antiapoptotic Bcl-2 and Bcl-w expression, increased the levels of proapoptotic Bax, Bak, Bad, Bik, and Bim expression, and activated caspase-3 and caspase-9 in MCF-7 BCSCs. In the presence of Bcl-2 siRNA, the apoptosis rate increased by 15% in cells treated with curcumin and MMC. The mitochondrial membrane potential decreased by approximately 20% in MCF-7 BCSCs undergoing the combination treatment of curcumin and MMC. The combination-induced decrease in Bcl-2 was regulated by the presence of the Wnt-specific inhibitor PFK115-584 and PI3k inhibitor LY294002. CONCLUSIONS:Our study indicates that curcumin might represent a novel therapeutic agent for treating breast cancer chemoresistance induced by MMC.
journal_name
Cancer Cell Intjournal_title
Cancer cell internationalauthors
Zhou QM,Sun Y,Lu YY,Zhang H,Chen QL,Su SBdoi
10.1186/s12935-017-0453-3subject
Has Abstractpub_date
2017-09-26 00:00:00pages
84issn
1475-2867pii
453journal_volume
17pub_type
杂志文章abstract：Background:Hepatocellular carcinoma (HCC) is one of the most common malignancies with a high morbidity and mortality worldwide. MicroRNAs are key regulators of HCC genesis. However, the regulatory role and underlying mechanisms of microRNA in HCC is still limited. Methods:Cyclin B1 (CCNB1) mRNA levels were examined in...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-0729-x
更新日期：2019-01-14 00:00:00
abstract：:N6-methyl-adenosine(m6A) modification emerges as an abundant and dynamic regulation throughout the Eukaryotic transcriptome. Dysregulation of the m6A regulators has increasingly been found in many neoplasms. It is reasonable to believe that m6A changes the fate of cancer cells and subsequently affected all aspects of ...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-020-01445-y
更新日期：2020-07-29 00:00:00
abstract：Background:Esophageal squamous cell carcinoma (ESCC) is one of the most prevalent malignancies and a major cause of cancer related death worldwide, especially in China. Cell lines are widely used disease models for basic medical research, however, well characterized ESCC cell models from China were seldom reported. Mis...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01268-x
更新日期：2020-05-24 00:00:00
abstract：BACKGROUND:The expression of major histocompatibility complex class II (MHCII) antigens in both mouse and human tumors is rare, and these antigens are not easily inducible by IFN-gamma (IFNg). Since MHCII may play an important role in the development of host antitumor immune response, we explored the possibility of res...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-3-4
更新日期：2003-03-25 00:00:00
abstract：BACKGROUND:Avenanthramides (AVN) are a relatively unstudied family of phytochemicals that could be novel chemotherapeutics. These compounds, found in oats, are non-toxic to healthy cells and have been shown to reduce viability of human colon and liver cancers in vitro. However, these studies do not elucidate a molecula...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-017-0464-0
更新日期：2017-10-18 00:00:00
abstract：: Autoimmune phenomena were identified in many different cases of hematological diseases and solid tumors, which may be due to alterations in the expression and function of the NF-κB signaling pathway. Recently, a number of studies have shown that the deletion or mutation of A20, a negative regulator of NF-κB, is frequ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-12-44
更新日期：2012-11-07 00:00:00
abstract：UNLABELLED: BACKGROUND:Anticancer research resulted in the discovery of a promising antimitotic metabolite, 2-methoxyestradiol. 2-Methoxyestradiol-bis-sulphamate, a bis-sulphamoylated analogue exerts antiproliferative- and antimitotic activity. Investigating the anticancer potential of 2-methoxyestradiol-bis-sulphamat...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-12-37
更新日期：2012-08-20 00:00:00
abstract：BACKGROUND:Endometrial cancer is an invasive gynecological cancer prevalent in the world. The pathogenesis of endometrial cancer is related to multiple levels of regulation, referring to oestrogen, tumor-suppressor gene (e.g. PTEN) or microRNAs (e.g. miR-23a and miR-29b). Metapristone is a hormone-related drug, which i...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01682-1
更新日期：2021-01-07 00:00:00
abstract：Background:Inflammation is one of a main reason for colon cancer progression and poor prognosis. The high-mobility group box-1 (HMGB1) and glutathione peroxidase 4 (GPX4) are responsible for inflammation, but the relationship between HMGB1 and GPX4 remains unknown about inflammation in colon cancer. Methods:RT-qPCR wa...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01289-6
更新日期：2020-05-29 00:00:00
abstract：BACKGROUND:Non-small cell lung cancer comprises the majority of lung cancer cases and is insensitive to chemotherapy. Most patients develop drug resistance. Recently, tetrandrine (TET), a bis-benzylisoquinoline alkaloid, was identified as a novel anti-cancer agent. However, the effect of tetrandrine combined with cispl...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-017-0410-1
更新日期：2017-03-27 00:00:00
abstract：BACKGROUND:The digallic acid (DGA) purified from Pistacia lentiscus. L fruits was investigated for its antiproliferative and apoptotic activities on human lymphoblastoid TK6 cells. METHODS:We attempt to characterize the apoptotic pathway activated by DGA. Apoptosis was detected by DNA fragmentation, PARP cleavage and ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-12-26
更新日期：2012-06-11 00:00:00
abstract：Background:Immunotherapies that targeting programmed cell death 1 (PD-1) and programmed death-ligand 1 (PD-L1) have obtained prominent success in breast cancer (BC). However, not all the patients benefit from the antibody therapy. This study aimed to identify PD-1/PD-L1 correlated genes and pathways as well as investig...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-0955-2
更新日期：2019-09-09 00:00:00
abstract：Background:Recent studies have demonstrated that the kallikrein and kallikrein-related peptidases (KLKs) exhibit aberrant expression in patients with colorectal cancer (CRC) and might be considered as potential prognostic biomarkers of CRC. However, inconsistent findings have been reported, which promote us to summariz...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01350-4
更新日期：2020-06-22 00:00:00
abstract：BACKGROUND:Celecoxib is a selective cyclooxygenase (COX)-2 inhibitor that has been reported to reduce the risk of breast cancer. In our previous study, celecoxib induced apoptosis and caused cell cycle arrest at the G0/G1 phase in the breast cancer cell line MDA-MB-231, and its effects were mediated by downregulation o...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-13-14
更新日期：2013-02-12 00:00:00
abstract：:Survivin is the smallest member of the Inhibitor of apoptosis (IAP) family of proteins, involved in inhibition of apoptosis and regulation of cell cycle. These functional attributes make Survivin a unique protein exhibiting divergent functions i.e. regulating cell proliferation and cell death. Expression pattern of Su...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-016-0326-1
更新日期：2016-06-23 00:00:00
abstract：BACKGROUND:Despite the modern therapies available for treating glioblastoma multiforme (GBM), it is still a deadly disease. The development of new therapeutic strategies for the management of gliomas is therefore crucial. The present study is designed to analyze the therapeutic potentials of synthetic compound N-(2-hyd...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-014-0133-5
更新日期：2014-11-30 00:00:00
abstract：Background:Osteosarcoma (OS) is still a disease with high mortality from malignant tumors in children and adolescents. Due to its poor treatment, this study explored the involvement of lncRNA ZFAS1/microRNA-135a (miR-135a)/apurinic/apyrimidinic exonuclease 1 (APEX1) axis in the regulation of OS growth and metastasis. ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-1049-x
更新日期：2019-12-03 00:00:00
abstract：:Cancer stem-like cells exhibit the multi-functional roles to survive and persist for a long period in the minimal residual disease after the conventional anti-cancer treatments. Cancer stem-like cells of solid malignant tumors which highly express CD44v8-10, the variant isoform of CD44 generated by alternative splicin...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-017-0393-y
更新日期：2017-02-13 00:00:00
abstract：BACKGROUND:ErbB3-binding protein 1 (Ebp1) is a member of the PA2G4 family of proliferation-regulated proteins that is expressed in multiple malignant and non-malignant cells. ErbB3 and other members of the EGFR family have been implicated in cancer progression, it however remains unknown whether Ebp1 participate in pro...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-8-18
更新日期：2008-11-24 00:00:00
abstract：Background:Glioma is the most common central nervous system (CNS) tumour. p62, an important autophagy adaptor, plays a crucial role in cancer. However, the role of p62 in the progression of glioma is poorly characterized. Methods:We examined the expression of p62 in glioma tissues and cell lines. Then we investigated ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-1004-x
更新日期：2019-11-06 00:00:00
abstract：Background:At the present time, colorectal cancer (CRC) is still known as a disease with a high mortality rate. Theranostics are flawless scenarios that link diagnosis with therapy, including precision medicine as a critical platform that relies on the development of biomarkers particularly "liquid biopsy". Circulating...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-020-01389-3
更新日期：2020-07-06 00:00:00
abstract：BACKGROUND:Phenethyl isothiocyanate (PEITC), present naturally in cruciferous vegetables, is a chemopreventive agent. It blocks initiation and post-initiation progression of carcinogenesis. Mechanism study in human prostate cancer cells revealed that PEITC is a dual inhibitor of aberrant DNA hypermethylation and histon...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-14-30
更新日期：2014-03-31 00:00:00
abstract：BACKGROUND:The aim of this study is to explore the expression of alpha-synuclein (α-synuclein) in benign, atypical, and anaplastic meningiomas and determine its role in the malignant progression of meningiomas. METHODS:Expression of α-synuclein was measured in 44 meningioma samples by real-time PCR analysis. The effec...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-016-0361-y
更新日期：2016-11-15 00:00:00
abstract：BACKGROUND:Recent studies have reported the involvement of microRNA-29 (miR-29) family members in human cancers through their ability to regulate cellular functions. The present study investigated biological function of miR-29b in colorectal cancer (CRC). METHODS:CRC tissues and adjacent normal tissues were collected ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01700-2
更新日期：2021-01-06 00:00:00
abstract：BACKGROUND:The incidence and mortalities from cancers remain on the rise worldwide. Despite significant efforts to discover and develop novel anticancer agents, many cancers remain in the unmet need category. As such, efforts to discover and develop new and more effective and less toxic agents against cancer remain a t...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-017-0404-z
更新日期：2017-03-07 00:00:00
abstract：Background:Pancreatic cancer (PC) remains one of the most aggressive cancers worldwide. However, genetic factors underlying PC susceptibility remain largely unclear. Long noncoding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) acts as an oncogene and its genetic variation has been linked to many cancers. However, ...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-019-0761-x
更新日期：2019-02-28 00:00:00
abstract：Background:Acute lymphoblastic leukemia (ALL) is an aggressive hematopoietic malignancy that is most commonly observed in children. Alantolactone (ALT) has been reported to exhibit anti-tumor activity in different types of cancer. The aim of the present study was to investigate the anti-tumor activity and molecular mec...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-020-01537-9
更新日期：2020-09-09 00:00:00
abstract：BACKGROUND:Gastric cancer is one of the common cancers seen in south India. Unfortunately more than 90% are advanced by the time they report to a tertiary centre in the country. There is an urgent need to characterize these cancers and try to identify potential biomarkers and novel therapeutic targets. MATERIALS AND M...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/1475-2867-10-45
更新日期：2010-11-24 00:00:00
abstract：:Osteosarcoma (OS) is one of the most prevalent malignant cancers with lower survival and poor overall prognosis mainly in children and adolescents. Identifying the molecular mechanisms and OS stem cells (OSCs) as new concepts involved in disease pathogenesis and progression may potentially lead to new therapeutic targ...
journal_title：Cancer cell international
pub_type： 杂志文章,评审
doi：10.1186/s12935-018-0654-4
更新日期：2018-10-16 00:00:00
abstract：Background:T-acute lymphoblastic leukemia (T-ALL) was a hematological malignancy characterized by the accumulation of immature T cells in bone marrow and peripheral blood. In this study, we tried to explore the physiological role of CD59 in T-ALL. Methods:In this study, we collected the bone marrow samples from 17 T-A...
journal_title：Cancer cell international
pub_type： 杂志文章
doi：10.1186/s12935-018-0714-9
更新日期：2019-01-08 00:00:00
